Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,356,035 C→G 10.7% intergenic (+302/‑90) PP_1180 → / → PP_1181 hypothetical protein/two‑component system response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,356,0350CG10.7% 123.2 / 5.5 46intergenic (+302/‑90)PP_1180/PP_1181hypothetical protein/two‑component system response regulator
Reads supporting (aligned to +/- strand):  ref base C (22/19);  new base G (3/2);  total (25/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.64e-01

TTTGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACC‑GCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAGACGACCAGCGCCTCGCCGAAC  >  NC_002947/1355899‑1356182
                                                                                                                                         |                                                                                                                                                   
tatGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACC‑GCCCGCGGGGCGCTc                                                                                                                                         >  1:232864/3‑149 (MQ=255)
    cAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACC‑GCCCGCGGGGCGCTCGGtc                                                                                                                                     >  6:348758/1‑149 (MQ=255)
         tttttCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACC‑GCCCGCGGGGCGCTCGGTCTCg                                                                                                                                  >  5:20915/1‑147 (MQ=255)
          ttttCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACC‑GCCCGCGGGGCGCTCGGTCTCGCAg                                                                                                                               >  6:299390/1‑149 (MQ=255)
                gCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACC‑GCCCGCGGGGCGCTCGGTCTCGCAGGGcccc                                                                                                                         >  6:142901/1‑149 (MQ=255)
                  aCCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACC‑GCCCGCGGGGCGCTCGGTCTCGCAGGGccccc                                                                                                                        >  6:149366/1‑148 (MQ=255)
                        aaGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACC‑GCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTa                                                                                                                  <  5:207791/148‑1 (MQ=255)
                          gcacTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACC‑GCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACa                                                                                                                >  6:141375/4‑148 (MQ=255)
                          gCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACC‑GCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACAt                                                                                                               >  4:42191/1‑149 (MQ=255)
                           cccTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACC‑GCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACAt                                                                                                               >  6:77308/1‑148 (MQ=255)
                            ccTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACC‑GCCCGCGGGGCGCTCGGTCTCGc                                                                                                                                 <  4:246119/129‑1 (MQ=255)
                            ccTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACC‑GCCCGCGGGGCGCTCGGTCTCGc                                                                                                                                 >  3:246119/1‑129 (MQ=255)
                             cTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACC‑GCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCa                                                                                                            <  3:42191/149‑1 (MQ=255)
                             cTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACC‑GCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCa                                                                                                            <  5:141375/149‑1 (MQ=255)
                                gggCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACC‑GCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATa                                                                                                          <  2:232864/148‑1 (MQ=255)
                                      ttttttttGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACC‑GCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAg                                                                                                   >  1:280650/1‑149 (MQ=255)
                                       tttttttGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACC‑GCCagcg                                                                                                                                                 >  1:172672/1‑102 (MQ=255)
                                       tttttttGGGCGCGAAGCCTGTAACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACC‑GCCcgcg                                                                                                                                                 <  2:172672/102‑1 (MQ=255)
                                            ttGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACC‑GCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTGcc                                                                                              <  5:142901/148‑1 (MQ=255)
                                                     aaGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACC‑GCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTGCCCTGACACGAt                                                                                    <  5:149366/149‑1 (MQ=255)
                                                              acaAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACC‑GCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTGCCCTGACACGATCCCAAGaa                                                                            <  5:299390/148‑1 (MQ=255)
                                                               caAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACC‑GCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTGCCCTGACACGATCCCAAGaaca                                                                          >  4:13041/1‑149 (MQ=255)
                                                                  aaaaCGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACC‑GCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTGCCCTGACACGATCCCAAGaacaac                                                                        >  8:42076/1‑148 (MQ=255)
                                                                             gTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACC‑GCCCGCGCGGCGCTCGGTCTCGCAGGGCCCCCCATCTAAATTCATACTCTAAGCAGCACTGACACGAACCCAAGAACAACAAGGCCAAAGc                                                             >  2:165225/1‑148 (MQ=39)
                                                                                 aGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACC‑GCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATgg                                                        <  2:175575/149‑1 (MQ=255)
                                                                                        ggTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACC‑GCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCAccc                                                 >  7:48418/1‑149 (MQ=255)
                                                                                              gCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACC‑GCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATAcc                                           >  5:100531/1‑149 (MQ=255)
                                                                                               cGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACC‑GCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATAccc                                          <  7:42076/149‑1 (MQ=255)
                                                                                                gtgtCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACC‑GCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATAcccc                                         <  2:280650/149‑1 (MQ=255)
                                                                                                  gtCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACC‑GCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCg                                        >  8:61094/1‑148 (MQ=255)
                                                                                                  gtCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACC‑GCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCg                                        >  5:333545/1‑148 (MQ=255)
                                                                                                   tCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACC‑GCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGc                                       <  6:20915/148‑1 (MQ=255)
                                                                                                      gAAGATCTGGGGTGCCTGGGAGATCGAGCACC‑GCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATcc                                   >  7:246612/1‑149 (MQ=255)
                                                                                                          aTCTGGGGTGCCTGGGAGATCGAGCACCGGCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCa                                >  1:20938/1‑149 (MQ=255)
                                                                                                               gggTGCCTGGGAGATCGAGCACC‑GCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCAtcgtc                           <  5:77308/148‑1 (MQ=255)
                                                                                                               gggTGCCTGGGAGATCGAGCACC‑GCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCAtcgtc                           >  6:23235/1‑148 (MQ=255)
                                                                                                               gggTGCCTGGGAGATCGAGCACC‑GCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCAtcg                             <  6:100531/146‑1 (MQ=255)
                                                                                                               gggTGCCTGGGAGATCGAGCACC‑GCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCAtcg                             <  2:20938/146‑1 (MQ=255)
                                                                                                                gggcccTGCGAGACCGAGCGCC‑CCGCGGGCGGTGCTCGATCTCCCA‑GGCACCCCATCTACATTCATACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGaa                        >  2:148466/5‑149 (MQ=21)
                                                                                                                        ggAGATCGAGCACC‑GCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAGACGAcc                 <  6:333545/149‑1 (MQ=255)
                                                                                                                            accGAGCGCC‑CCGCGGGCGGTGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGaa                        >  3:157458/3‑138 (MQ=255)
                                                                                                                            accGAGCGCC‑CCGCGGGCGGTGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGaa                        <  6:56409/136‑1 (MQ=255)
                                                                                                                            accGAGCGCC‑CCGCGGGCGGTGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGaa                        <  4:157458/136‑1 (MQ=255)
                                                                                                                            accGAGCGCC‑CCGCGGGCGGTGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGaa                        >  5:56409/3‑138 (MQ=255)
                                                                                                                                 gCACC‑GCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAGACGACCAGCGCCTCg        <  3:13041/149‑1 (MQ=255)
                                                                                                                                   aCC‑GCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAGACGACCAGCGCCTCGcc      <  7:61094/149‑1 (MQ=255)
                                                                                                                                        cccgcGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCCTCGAAGACGACCAGCGCCTCGCCGaa   >  4:134319/1‑148 (MQ=255)
                                                                                                                                         ccgcGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAGACGACCAGCGCCTCGCCGAAc  >  2:166920/1‑148 (MQ=255)
                                                                                                                                         |                                                                                                                                                   
TTTGCAGTATTTTTCTGCACCGTGAAGCCCTTGGGCAGTTTTTTTTGGGCGCGAAGCCTGTCACAAAAAACGGTTCAGTCGAGGGGGCGGTAGGGCGTGTCTGAAGATCTGGGGTGCCTGGGAGATCGAGCACC‑GCCCGCGGGGCGCTCGGTCTCGCAGGGCCCCCCATCTACATTCATACTCTCAGCTGCCCTGACACGATCCCAAGAACAACAAGGCCAAAGCCATGGACCACCCCATACCCCGCATCCTCATCGTCGAAGACGACCAGCGCCTCGCCGAAC  >  NC_002947/1355899‑1356182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑