| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 166,581 | A→T | 7.7% | I109N (ATT→AAT) | pntAA ← | pyridine nucleotide transhydrogenase subunit alpha |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 166,581 | 0 | A | T | 7.7% | 228.2 / 7.3 | 78 | I109N (ATT→AAT) | pntAA | pyridine nucleotide transhydrogenase subunit alpha |
| Reads supporting (aligned to +/- strand): ref base A (35/37); new base T (2/4); total (37/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.78e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.35e-01 | |||||||||||
GGTCATCAGCATTGGCATGAAGCGTGGGTAGTGATGGGCTGCCAGCAACACGGCCTTGTAGCCGGCGATGTTGGCCTGCGACGACAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCACCGTTGAAGGGGTTGAGCATGCCCACCAGAAGGCTGCCGCTGTTGATCAGGGCCAGTTCCTGGTCGTTGGGGGCGACCACTTTGAGTACCAATTGGGCGCCGTAGGCATCGGCGGC > NC_002947/166436‑166729 | ggTCATCAGCATTGGCATGAAGCGTGCGTAGTGATGGGCTGCCAGCAACACGGCCATGTAGCCGGCTATGTTGGCCAGCGACGACAGCACGTCCACGCTCTGCGCCCGCGAGGTTAGCGGCGCGCCTTCCAGGGAGAAGGCGGTAATGc > 6:197597/1‑149 (MQ=255) gTCATCAGCATTGGCATGAAGCGTGGGTAGTGATGGGCTGCCAGCAACACGGCCTTGTAGCCGGCGATGTTGGCCTGCGACGACAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGcc > 2:76830/1‑149 (MQ=255) atcaGCATTGGCATGAAGCGTGGGTAGTGATGGGCTGCCAGCAACACGGCCTTGTAGCCGGCGATGTTGGCCTGCGACGACAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTTCAGGGCGAAGGCGGTAATGCcgcg > 1:326677/1‑149 (MQ=255) atcaGCATTGGCATGAAGCGTGGGTAGTGATGGGCTGCCAGCAACACGGCCTTGTAGCCGGCGATGTTGGCCTGCGACGACAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCcgcg > 4:151946/1‑149 (MQ=255) tcaGCATTGGCATGAAGCGTGGGTAGTGATGGGCTGCCAGCAACACGGCCTTGTAGCCGGCGATGTTGGCCTGCGACGACAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGt > 3:16519/1‑149 (MQ=255) aGCATTGGCATGAAGCGTGGGTAGTGATGGGCTGCCAGCAACACGGCCTTGTAGCCGGCGATGTTGGCCTGCGACGACAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTc < 3:183439/149‑1 (MQ=255) gCATTGGCATGAAGCGTGGGTAGTGATGGGCTGCCAGCAACACGGCCTTGTAGCCGGCGATGTTGGCCTGCGACGACAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCg < 1:316271/149‑1 (MQ=255) cATTGGCATGAAGCGTGGGTAGTGATGGGCTGCCAGCAACACGGCCTTGTAGCCGGCGATGTTGGCCTGCGACGACAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCgg < 8:269920/149‑1 (MQ=255) cATTGGCATGAAGCGTGGGTAGTGATGGGCTGCCAGCAACACGGCCTTGTAGCCGGCGATGTTGGCCTGCGACGACAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAACGCGGCATTACCGCCTTCgc < 5:168234/149‑2 (MQ=255) aTTGGCATGAAGCGTGGGTAGTGATGGGCTGCCAGCAACACGGCCTTGTAGCCGGCGATGTTGGCCTGCGACGACAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑c < 7:184730/149‑1 (MQ=255) tGAAGCGTGGGTAGTGATGGGCTGCCAGCAACACGGCCTTGTAGCCGGCGATGTTGGCCTGCGACGACAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGCCGAACGCGGCATTACCGCCTTCGc‑cctggaag < 3:277665/149‑7 (MQ=38) aaGCGTGGGTAGTGATGGGCTGCCAGCAACACGGCCTTGTAGCCGGCGATGTTGGCCTGCGACGACAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGcc < 3:35059/149‑1 (MQ=255) tGGGTAGTGATGGGCTGCCAGCAACACGGCCTTGTAGCCGGCGATGTTGGCCTGCGACGACAGCACGTCCAGCAACTCCCCCCCCGAGTTTCCCGCCCCGCATTCCAGGGCAAAGGCGTTAATGCCCCTTACGG‑CCAAATTGCCTATCa > 4:225536/1‑149 (MQ=255) ggTAGTGATGGGCTGCCAGCAACACGGCCTTGTAGCCGGCGATGTTGGCCTGCGACGACAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAg < 6:182500/148‑1 (MQ=255) gTGATGGGCTGCCAGCAACACGGCCTTGTAGCCGGCGATGTTGGCCTGCGACGACAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCa < 6:284395/148‑1 (MQ=255) gggCTGCCAGCAACACGGCCTTGTAGCCGGCGATGTTGGCCTGCGACGACAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCACCGtt > 5:109230/1‑148 (MQ=255) ggCTGCCAGCAACACGGCCTTGTAGCCGGCGATGTTGGCCTGCGACGACAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAAGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGtt < 2:317688/140‑1 (MQ=255) ggCTGCCAGCAACACGGCCTTGTAGCCGGCGATGTTGGCCTGCGACGACAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAAGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGtt > 1:317688/1‑140 (MQ=255) cTGCCAGCAACACGGCCTTGTAGCCGGCGATGTTGGCCTGCGACGACAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCACCGTTGAAg > 8:247017/1‑149 (MQ=255) cTGCCAGCAACACGGCCTTGTAGCCGGCGATGTTGGCCTGCGACGACAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCACCGTTGAAg < 7:247017/149‑1 (MQ=255) tGCCAGCAACACGGCCTTGTAGCCGGCGATGTTGGCCTGCGACGACAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCACCGTTGAAgg > 8:211655/1‑149 (MQ=255) ccAGCAACACGGCCTTGTAGCCGGCGATGTTGGCCTGCGACGTCAGCACGTGCTGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGGCGCGTTCGT‑CCATCTTGCCGATCAGTTGACCGTTGAAgg < 5:197597/147‑1 (MQ=255) ccAGCAACACGGCCTTGTAGCCGGCGATGTTGGCCTGCGACGACAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCACCGTTGAAggg < 6:141170/148‑1 (MQ=255) cAGCAACACGGCCTTGTAGCCGGCGATGTTGGCCTGCGACGACAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCACCGTTGAAGGGGt > 4:186180/1‑149 (MQ=255) aGCAACACGGCCTTGTAGCCGGCGATGTTGGCCTGCGACGACAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCACCGTTGAAGGGGtt > 3:244774/1‑149 (MQ=255) aGCAACACGGCCTTGTAGCCGGCGATGTTGGCCTGCGACGACAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCACCGTTGAAGGGGtt < 5:327965/149‑1 (MQ=255) gCAACACGGCCTTGTAGCCGGCGATGTTGGCCTGCGACGACAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCACCGTTGAAGGGGtt > 5:208189/1‑148 (MQ=255) gCAACACGGCCTTGTAGCCGGCGATGTTGGCCTGCGACGACAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCACCGTTGAAGGGGtt > 3:61386/1‑148 (MQ=255) cAACACGGCCTTGTAGCCGGCGATGTTGGCCTGCGACGACAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAACGCGGCATTACCGCCTTCgcc < 2:248618/114‑3 (MQ=255) cAACACGGCCTTGTAGCCGGCGATGTTGGCCTGCGACGACAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAACGCGGCATTACCGCCTTCgcc > 1:248618/1‑112 (MQ=255) aaCACGGCCTTGTAGCCGGCGATGTTGGCCTGCGACGACAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCACCGTTGAAg < 3:241831/141‑1 (MQ=255) aaCACGGCCTTGTAGCCGGCGATGTTGGCCTGCGACGACAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCACCGTTGAAg > 4:241831/1‑141 (MQ=255) aaCACGGCCTTGTAGCCGGCGATGTTGGCCTGCGACGACAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCACCGTTGAAGGGGTTGAg < 3:186180/149‑1 (MQ=255) acacGGCCTTGTAGCCGGCGATGTTGGCCTGCGACGACAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCACCGTTGAAGGGGTTGa > 6:249391/1‑147 (MQ=255) acacGGCCTTGTAGCCGGCGATGTTGGCCTGCGACGACAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCACCGTTGAAGGGGTTGa < 5:249391/147‑1 (MQ=255) cGGCCTTGTAGCCGGCGATGTTGGCCTGCGACGACAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCtt < 5:33178/115‑1 (MQ=255) cGGCCTTGTAGCCGGCGATGTTGGCCTGCGACGACAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCtt > 6:33178/1‑115 (MQ=255) ccTTGTAGCCGGCGATGTTGGCCTGCGACGACAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCACCGTTGAAGGGGTTGAGCATGccc < 4:16519/149‑1 (MQ=255) ccTTGTAGCCGGCGATGTTGGCCTGCGACGACAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCACCGTTGAAGGGGTTGAGCATGcc > 2:6999/1‑148 (MQ=255) ccTTGTAGCCGGCGATGTTGGCCTGCGACGACAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCACCGTTGAAGGGGTTGAGCATGcc > 2:12553/1‑148 (MQ=255) ttGTAGCCGGCGATGTTGGCCTGCGACGACAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCACCGTTGAAGGGGTTGAGCATGCccac > 4:199108/1‑149 (MQ=255) gTAGCCGGCGATGTTGGCCTGCGACGACAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGGCCCATCTTGCCGATCAGTTCACCGTTGAAGGGGTTGAGCATGCccac < 1:76830/148‑1 (MQ=255) gTAGCCGGCGATGTTGGCCTGCGACGACAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCACCGTTGAAGGGGTTGa > 6:282384/1‑137 (MQ=255) gTAGCCGGCGATGTTGGCCTGCGACGACAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCACCGTTGAAGGGGTTGa < 5:282384/137‑1 (MQ=255) gTAGCCGGCGATGTTGGCCTGCGACGACAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCACCGTTGAAGGGGTTGAGCATGCccac < 1:12553/147‑1 (MQ=255) gTAGCCGGCGATGTTGGCCTGCGACGACAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCACCGTTGAAGGGGTTGAGCATGCccac < 1:6999/147‑1 (MQ=255) aGCCGGCGATGTTGGCCTGCGACGACAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCACCGTTGAAGGGGTTGAGCATGCCCACCAg > 1:55614/1‑148 (MQ=255) gCCGGCGATGTTGGCCTGCGACGACAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCACCGTTGAAGGGGTTGAGCATGCCCACCAGa < 4:61386/148‑1 (MQ=255) gATGTTGGCCTGCGACGACAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCACCGTTGAAGGGGTTGAGCATGCCCACCAGAAGGCTGc > 7:229778/1‑149 (MQ=255) aTGTTGGCCTGCGACGACAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCACCGTTGAAGGGGTTGAGCATGCCCACCAGAAGGCTGcc < 3:127953/149‑1 (MQ=255) ggCCTGCGACGACAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAACGCGGCATTACCGCCTTCGc‑cc > 2:187477/1‑88 (MQ=37) ggCCTGCGACGACAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAACGCGGCATTACCGCCTTCGc‑cc < 1:187477/88‑1 (MQ=37) ggCCTGCGACGACAGCACGGCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTCCCGATCAGTTCACCGTTGAAGGGGTTGAGCATGCCCACCAGAAGGCTGCCGCTg < 6:258487/148‑1 (MQ=255) gCCTGCGACGACAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCACCGTTGAAGGGGTTGAGCATGCCCACCAGAAGGCTGCCGCTGt < 3:151946/148‑1 (MQ=255) acgacAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCACCGTTGAAGGGGTTGAGCATGCCCACCAGAAGGCTGCCGCTGtt > 8:178349/1‑142 (MQ=255) acgacAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCACCGTTGAAGGGGTTGAGCATGCCCACCAGAAGGCTGCCGCTGtt < 7:178349/142‑1 (MQ=255) acgacAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCACCGTTGAAGGGGTTGAGCATGCCCACCAGAAGGCTGCCGCTGGTGATAAgg > 6:287056/1‑149 (MQ=255) acAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCACCGTTGAAGGGGTTGAGCATGCCCACCAGAAGGCTGCCGCTGTTGATCAGGGc > 4:94410/1‑148 (MQ=255) gCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCTCCGTTGAAGGGGTTGAGCATGCCCACCAGAAGGCTGCCGCTGTTGATCAGGGCCAGt > 5:106973/1‑149 (MQ=255) gCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCACCGTTGAAGGGGTTGAGCATGCCCACCAGAAGGCTGCCGCTGTTGATCAGGGCCAGt > 7:35795/1‑149 (MQ=255) cACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCACCGTTGAAgggg < 4:42131/103‑1 (MQ=255) aCGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCACCGTTGAAGGGGTTGAGCATGCCCACCAGAAGGCTGCCGCTGTTGATCAGGGCCAGTTc > 6:238786/1‑149 (MQ=255) aCGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCACCGTTGAAGGGGTTGAGCATGCCCACCAGAAGGCTGCCGCTGTTGATCAGGGCCAGTTc > 5:139410/1‑149 (MQ=255) cAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCACCGTTGAAGGGGTTGAGCATGCCCACCAGaa > 8:136816/1‑116 (MQ=255) cAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCACCGTTGAAGGGGTTGAGCATGCCCACCAGAAGGCTGCCGCTGTTGATCAGGGCCAGTTCCTgg < 7:211655/148‑1 (MQ=255) ctGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCACCGTTGAAGGGGTTGAGCATGCCCACCAGAAGGCTGCCGCTGTTGATCAGGGCCAGTTCCTGGTCGTTgg > 3:313036/1‑149 (MQ=255) tGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCACCGTTGAAGGGGTTGAGCATGCCCACCAGAAGGCTGc > 4:77470/1‑115 (MQ=255) tGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCACCGTTGAAGGGGTTGAGCATGCCCACCAGAAGGCTGc < 3:77470/115‑1 (MQ=255) cgcCCGCGAGGTTCGCGGCGCGGCTTTCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCACCGTTGCAGGGGTTGAGCATGCCCACCAGAAGGCTGCCGCTGTTGATCAGGGCCAGTTCCTGGTCGTTggg < 2:326677/147‑1 (MQ=255) cgcCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCACCGTTGAAGGGGTTGAGCATGCCCACCAGAAGGCTGCCGCTGTTGATCAGGGCCAGTTCCTGGTCGTTggggg > 6:240671/1‑149 (MQ=255) cgcCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCACCGTTGAAGGGGTTGAGCATGCCCACCAGAAGGCTGCCGCTGTTGATCAGGGCCAGTTCCTGGTCGTTggg < 8:35795/147‑1 (MQ=255) gcCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCACCGTTGAAGGGGTTGAGCATGCCCACCAGAACGCTGCCGCTGTTGATCAGGGCCAGTTCCTGGTCGTTGGGGGc < 6:208189/149‑1 (MQ=255) ggcgcggcTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCACCGTTGAAGGGGTTGAGCATGCCCACCAGAAGGCTGCCGCTGTTGATCAGGGCCAGTTCCTGGTCGTTGGGGGCGACCACTTTGAGTAc < 5:240671/149‑1 (MQ=255) ggcgcggcTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCACCGTTGAAGGGGTTGAGCATGCCCACCAGAAGGCTGCCGCTGTTGATCAGGGCCAGTTCCTGGTCGTTGGGGGCGACCACTTTGAGTAc < 4:244774/149‑1 (MQ=255) cTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCACCGTTGAAGGGGTTGAGCATGCCCACCAGAAGGCTGCCGCTGTTGATCAGGGCCAGTTCCTGGTCGTTGGGGGCGACCACTTTGAGTACCAATTgg < 2:55614/149‑1 (MQ=255) ccAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCACCGTTGAAGGGGTTGAGCATGCCCACCAGAAGGCTGCCGCTGTTGATCAGGGCCAGTTCCTGGTCGTTGGGGGCGACCACTTTGAGTACCAATTGGgc < 5:238786/148‑1 (MQ=255) cGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCACCGTTGAAGGGGTTGAGCATGCCCACCAGAAGGCTGCCGCTGTTGATCAGGGCCAGTTCCTGGTCGTTGGGGGCGACCACTTTGAGt < 7:282581/131‑1 (MQ=255) cGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCACCGTTGAAGGGGTTGAGCATGCCCACCAGAAGGCTGCCGCTGTTGATCAGGGCCAGTTCCTGGTCGTTGGGGGCGACCACTTTGAGt > 8:282581/1‑131 (MQ=255) aTGCCGCGTTCGG‑CCATCTTGCCCATCAGTTCCCCGTTGCAGGGGTTGAGAATGCCCACCAGAAGGCTGCCGCTGTTGATCCGGGCCAGTTACTGGTCGTTGGGGGCGACCACTTTGATTACCAATTGGGCGCCGTAGGCATCggcggc < 5:287056/149‑1 (MQ=255) | GGTCATCAGCATTGGCATGAAGCGTGGGTAGTGATGGGCTGCCAGCAACACGGCCTTGTAGCCGGCGATGTTGGCCTGCGACGACAGCACGTCCAGGCTCTGCGCCCGCGAGGTTCGCGGCGCGGCTTCCAGGGCGAAGGCGGTAATGCCGCGTTCGG‑CCATCTTGCCGATCAGTTCACCGTTGAAGGGGTTGAGCATGCCCACCAGAAGGCTGCCGCTGTTGATCAGGGCCAGTTCCTGGTCGTTGGGGGCGACCACTTTGAGTACCAATTGGGCGCCGTAGGCATCGGCGGC > NC_002947/166436‑166729 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |