Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,382,537 T→A 8.3% D255V (GAC→GTC)  PP_1204 ← lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,382,5370TA 8.3% 137.2 / 3.1 48D255V (GAC→GTC) PP_1204lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (22/22);  new base A (2/2);  total (24/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.80e-01

AGGAACTCCTGCTGCAGCTCCGGATCGTTGCGGGTCAGCTCGATCAGGCTCTGTTCCATTTCACTGGCTTCCTCTTCAAGGCCCAGTTCGGACAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTGGCATCGCCTCGTACCGCATTGTCCTCGTCAAGTACTTGCAGGTGCAGGGTGCCGACGTGGTTGAGGTTCTGCTCGGAGAACGG  >  NC_002947/1382396‑1382685
                                                                                                                                             |                                                                                                                                                    
aGGAACTCCTGCTGCAGCTCCGGATCGTTGCGGGTCAGCTCGATCAGGCTCTGTTCCATTTCACTGGCTTCCTCTTCAAGGCCCAGTTCGGACAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCg                                                                                                                                                 >  7:71634/1‑147 (MQ=255)
aGGAACTCCTGCTGCAGCTCCGGATCGTTGCGGGTCAGCTCGATCAGGCTCTGTTCCATTTCACTGGCTTCCTCTTCAAGGCCCAGTTCGGACAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCg                                                                                                                                                 >  4:308766/1‑147 (MQ=255)
  gAACTCCTGCTGCAGCTCCGGATCGTTGCGGGTCAGCTCGATCAGGCTCTGTTCCATTTCACTGGCTTCCTCTTCAAGGCCCAGTTCGGACAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGa                                                                                                                                             <  5:211010/149‑1 (MQ=255)
    aCTCCTGCTGCAGCTCCGGATCGTTGCGGGTCAGCTCGATCAGGCTCTGTTCCATTTCACTGGCTTCCTCTTCAAGGCCCAGTTCGGACAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATc                                                                                                                                           <  6:76689/149‑1 (MQ=255)
     cTCCTGCTGCAGCTCCGGATCGTTGCGGGTCAGCTCGATCAGGCTCTGTTCCATTTCACTGGCTTCCTCTTCAAGGCCCAGTTCGGACAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGATGACCTCGAAGAtga                                                                                                                                          <  1:191963/149‑3 (MQ=255)
        ctgctgCAGCTCCGGATCGTTGCGGGTCAGCTCGATCAGGCTCTGTTCCATTTCACTGGCTTCCTCTTCAAGGCCCAGTTCGGACAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGc                                                                                                                                        >  5:111287/1‑148 (MQ=255)
           ctgcAGCTCCGGATCGTTGCGGGTCAGCTCGATCAGGCTCTGTTCCATTTCACTGGCTTCCTCTTCAAGGCCCAGTTCGGACAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTc                                                                                                                                      <  8:71634/147‑1 (MQ=255)
               aGCTCCGGATCGTTGCGGGTCAGCTCGATCAGGCTCTGTTCCATTTCACTGGCTTCCTCTTCAAGGCCCAGTTCGGACAGGCGCTTGACCCGGTGAACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGc                                                                                                                                >  1:161452/1‑149 (MQ=255)
                                      cTCGATCAGGCTCTGTTCCATTTCACTGGCTTCCTCTTCAAGGCCCAGTTCGGACAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTc                                                                                                                             <  4:77124/129‑1 (MQ=255)
                                         gATCAGGCTCTGTTCCATTTCACTGGCTTCCTCTTCAAGGCCCAGTTCGGACAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTgg                                                                                                      >  7:93081/1‑149 (MQ=255)
                                            cAGGCTCTGTTCCATTTCACTGGCTTCCTCTTCAAGGCCCAGTTCGGACAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGgcgc                                                                                                   >  2:238242/1‑149 (MQ=255)
                                               gCTCTGTTCCATTTCACTGGCTTCCTCTTCAAGGCCCAGTTCGGACAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGa                                                                                                >  5:284540/1‑149 (MQ=255)
                                                ctcgGTTCCATTTCACTGGCTTCCTCTTCACCGCCGAGTTCGGACAGGCGCTTGACCCGGTGCACCCACTGGCCGTCATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGAc                                                                                               <  7:81699/149‑1 (MQ=255)
                                                       ccATTTCACTGGCTTCCTCTTCAAGGCCCAGTTCGGACAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGa                                                                                                >  4:45128/1‑141 (MQ=255)
                                                       ccATTTCACTGGCTTCCTCTTCAAGGCCCAGTTCGGACAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGa                                                                                                <  3:45128/141‑1 (MQ=255)
                                                        cATTTCACTGGCTTCCTCTTCAAGGCCCAGTTCGGACAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTc                                                                                                                                      >  4:51686/1‑102 (MQ=255)
                                                        cATTTCACTGGCTTCCTCTTCAAGGCCCAGTTCGGACAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGc                                                                                       <  6:111287/149‑1 (MQ=255)
                                                          tttCACTGGCTTCCTCTTCAAGGCCCAGTTCGGACAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTg                                                                                     <  1:238242/149‑1 (MQ=255)
                                                                     tCCTCTTCAAGGCCCAGTTCGGACAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTGGCATCGCCTCg                                                                          >  4:273431/1‑149 (MQ=255)
                                                                      ccTCTTCAAGGCCCAGTTCGGACAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTGGCATCGCCTc                                                                           <  8:93081/147‑1 (MQ=255)
                                                                       ctctTCAAGGCCCAGTTCGGACAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTGGCATCGCCTCGTa                                                                        <  5:171219/149‑1 (MQ=255)
                                                                         ctTCAAGGCCCAGTTCGGACAGGCGCTTGACACGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGTTGGAGCTCACCAACAGGCTGGCATCGCCTCGTaac                                                                      >  4:100295/1‑147 (MQ=255)
                                                                          ttCAAGGCCCAGTTCGGACAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGAt                                                                                                                                            <  4:40001/78‑1 (MQ=255)
                                                                            cAAGGCCCAGTTCGGACAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTGGCATCGCCTCGTAcc                                                                      <  6:284540/146‑1 (MQ=255)
                                                                             aaGGCCCAGTTCGGACAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTGGCATCGCCTCGTACCGCa                                                                   <  3:308766/148‑1 (MQ=255)
                                                                                      ttCGGACAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTGGCATCGCCTCGTACCGCATTGTCCTCGt                                                         <  3:100295/149‑1 (MQ=255)
                                                                                        cGGACAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTGGCATCGCCTCGTACCGCATTGTCCTCGTc                                                        <  8:4316/148‑1 (MQ=255)
                                                                                          gACAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTGGCATCGCCTCGTACCGCATTGTCCTCGTCAAg                                                     >  1:322578/1‑149 (MQ=255)
                                                                                               gcgcTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAAc                                                                                           >  2:86750/1‑106 (MQ=255)
                                                                                               gcgcTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAAc                                                                                           <  1:86750/106‑1 (MQ=255)
                                                                                                      aCCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTGGCATCGCCTCGTACCGCATTGTCCTCGTCAAGTACTtgcaggtg                                         >  2:11421/1‑149 (MQ=255)
                                                                                                       cccGGTGCACCCACTGGCCGACATCATCA‑‑‑TCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTGGCATCGCCTCGTACCGCATTGTCCTCGTCAAGTACTtgcaggtgcagg                                     >  3:241317/1‑149 (MQ=255)
                                                                                                       cccGGTGCACCCACTGGCCGACATCATCA‑‑‑TCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTGGCATCGCCTCGTACCGCATTGTCCTCGTCAAGTACTtgcaggtgcagg                                     <  4:241317/149‑1 (MQ=255)
                                                                                                               aCCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTGGCATCGCCTCGTACCGCATTGTCCTCGTCAAGTACTtgcaggtgcagg                                     >  6:38689/1‑144 (MQ=255)
                                                                                                               aCCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTGGCATCGCCTCGTACCGCATTGTCCTCGTCAAGTACTtgcaggtgcagg                                     <  5:38689/144‑1 (MQ=255)
                                                                                                                 ccACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCTAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTGGCATCGCCTCGTACCGCATTGTCCTCGTCAAGTACTTGCAGGTGCAGGGTACCGa                              >  5:77524/1‑149 (MQ=255)
                                                                                                                    cTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTGGCATCGCCTCGTACCGCATTGTCCTCGTCAAGTTCTTGCAGGTGCAGGGTGCCGACGt                           <  5:146422/149‑1 (MQ=255)
                                                                                                                    cTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTGGCATCGCCTCGTACCGCATTGTCCTCGTCAAGTACTTGCAGGTGCAGGGTGCCGACGt                           <  2:322578/149‑1 (MQ=255)
                                                                                                                      ggCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTGGCATCGCCTCGTACCGCATTGTCCTCGTCAAGTACTTGCAGGTGCAGGGTGCCGACGTgg                         >  1:265677/1‑149 (MQ=255)
                                                                                                                        ccGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTg                                                                                     >  4:204840/1‑87 (MQ=255)
                                                                                                                          gACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTGGCATCGCCTCGTACCGCATTGTCCTCGTCAAGTACTTGCAGGTGCAGGGTGCCGACGTGGTTGa                     <  1:11421/149‑1 (MQ=255)
                                                                                                                                 tgatcTTCGATGACCTCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTGGCATCGCCTCGTACCGCATTGTCCTCGTCAAGTACTtgc                                              >  6:233869/3‑117 (MQ=255)
                                                                                                                                 tgatcTTCGATGACCTCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTGGCATCGCCTCGTACCGCATTGTCCTCGTCAAGTACTtgc                                              <  5:233869/115‑1 (MQ=255)
                                                                                                                                 tgatcTTCGATGACCTCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACAAACAGGCTGGCATAGCCTCGTACCGCATTGTCCTCGTAAAGTACTTGCAGGTGCAGGGTGCCGACGTGGTTGAGGTTCTg              >  5:340028/3‑149 (MQ=255)
                                                                                                                                    tcTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTGGCATCGCCTCGTACCGCATTGTCCTCGTCAAGTACTTGCAGGTGCAGGGTGCCGACGTgg                         <  1:281938/135‑1 (MQ=255)
                                                                                                                                    tcTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTGGCATCGCCTCGTACCGCATTGTCCTCGTCAAGTACTTGCAGGTGCAGGGTGCCGACGTgg                         >  2:281938/1‑135 (MQ=255)
                                                                                                                                    tcTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTGGCATCGCCTCGTACCGCATTGTCCTCGTCAAGTACTTGCAGGTGCAGGGTGCCGACGTGGTTGAGGTTCTGGTc           >  3:195541/1‑149 (MQ=255)
                                                                                                                                           ggTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTGGCATCGCCTCGTACCGCATTGTCCTCGTCAAGTACTTGCAGGTGCAGGGTGCCGACGTGGTTGAGGTTCTGCTCGGAGAAc    >  4:131881/1‑149 (MQ=255)
                                                                                                                                             tCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTGGCATCGCCTCGTACCGCATTGTCCTCGTCAAGTACTTGCAGGTGCAGGGTGCCGACGTGGTTGAGGTTCTGCTCGGAGAACgg  <  3:273431/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                    
AGGAACTCCTGCTGCAGCTCCGGATCGTTGCGGGTCAGCTCGATCAGGCTCTGTTCCATTTCACTGGCTTCCTCTTCAAGGCCCAGTTCGGACAGGCGCTTGACCCGGTGCACCCACTGGCCGACATCATCATCTTCGAGGTCATCGAAGATCAGCTCATGGGCTTCGAGCAGCTTATTGCGCAGGGTGGCGCTGACCAACAGGCTGGCATCGCCTCGTACCGCATTGTCCTCGTCAAGTACTTGCAGGTGCAGGGTGCCGACGTGGTTGAGGTTCTGCTCGGAGAACGG  >  NC_002947/1382396‑1382685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑