Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,383,626	G→T	7.3%	L469M (CTG→ATG) ‡	proS ←	proline‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,383,626	0	G	T	7.3%	201.5 / 2.8	64	L469M (CTG→ATG) ‡	proS	proline‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (30/29);  new base T (2/3);  total (32/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCTGGCACTTCAGCGCCTCG  >  NC_002947/1383478‑1383771                                                                                                                                                     |                                                                                                                                                  ttGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAg                                                                                                                                                   >  6:336335/1‑149 (MQ=255)   gATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAggg                                                                                                                                                 >  3:167046/1‑149 (MQ=255)    aTGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGc                                                                                                                                                >  1:120852/1‑149 (MQ=255)    aTGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGc                                                                                                                                                >  5:10939/1‑149 (MQ=255)         tGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGcgtc                                                                                                                                             >  8:301192/1‑147 (MQ=255)         tGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGcgtc                                                                                                                                             <  7:301192/147‑1 (MQ=255)            gCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTcc                                                                                                                                        <  4:332631/149‑1 (MQ=255)                  tttCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAgatga                                                                                                                                  >  4:276898/1‑149 (MQ=255)                      ttGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGcc                                                                                                                              >  8:87283/1‑149 (MQ=255)                        gTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGGCGGTTGCGTAGCGCAGTGGGACCAGAGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGccc                                                                                                                             <  5:239849/148‑1 (MQ=255)                         tCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCtt                                                                                                                           <  3:59483/149‑1 (MQ=255)                           gcgGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTgt                                                                                                                         >  4:89542/1‑149 (MQ=255)                            cgGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTgtc                                                                                                                        <  5:264249/149‑1 (MQ=255)                               tcgtcCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTgtcgtc                                                                                                                     <  3:109189/149‑1 (MQ=255)                                  tcCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCa                                                                                                                    >  6:148857/1‑147 (MQ=255)                                   ccAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAg                                                                                                                 <  3:276898/149‑1 (MQ=255)                                    cagcagCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAg                                                                                                                 <  2:120852/148‑1 (MQ=255)                                    cagcagCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGACGCCCTGGCTCCGTTCCAga                                                                                                                                     <  3:21276/128‑1 (MQ=39)                                    cagcagCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGACGCCCTGGCTCCGTTCCAga                                                                                                                                     >  4:21276/1‑128 (MQ=39)                                           aCTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAg                                                                                                                                      <  3:298657/120‑1 (MQ=255)                                           aCTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAg                                                                                                                                      >  4:298657/1‑120 (MQ=255)                                               cATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCACATGATGACCTTGTCGGCATAGCTCTGCTCGATg                                                                                                     >  6:110395/1‑149 (MQ=255)                                                aTAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGAt                                                                                                      <  4:103661/147‑1 (MQ=255)                                                aTAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGAt                                                                                                      >  3:103661/1‑147 (MQ=255)                                                   gCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAg                                                                                                 <  7:87283/149‑1 (MQ=255)                                                    ccGGCCGCAGTCAGTTCTGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGc                                                                                                <  8:294437/149‑1 (MQ=255)                                                      ggcggcAGTCAGTTCGGCATACTGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTg                                                                                              <  6:10939/149‑1 (MQ=255)                                                          gcAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCGTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAAc                                                                                          <  6:333964/149‑1 (MQ=255)                                                           cagtcagtTCGGCATACAGCTTGTCGGTTGCTTCGAGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCaa                                                                                           <  4:167046/147‑1 (MQ=255)                                                             gtcagtTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACca                                                                                        >  6:91267/1‑148 (MQ=255)                                                                 gtTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACgg                                                                                   >  4:275327/1‑149 (MQ=255)                                                                 gtTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACgg                                                                                   >  8:276442/1‑149 (MQ=255)                                                                  ttCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAg                                                                                                 <  8:298188/134‑1 (MQ=255)                                                                  ttCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAg                                                                                                 >  7:298188/1‑134 (MQ=255)                                                                        aTACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGc                                                                                                   <  7:226340/126‑1 (MQ=255)                                                                        aTACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGc                                                                                                   >  8:226340/1‑126 (MQ=255)                                                                        aTACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGc                                                                            >  7:87583/1‑149 (MQ=255)                                                                        aTACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGc                                                                            >  3:75717/1‑149 (MQ=255)                                                                           cAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCaa                                                                         <  5:336335/149‑1 (MQ=255)                                                                           cAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCaa                                                                         <  5:91267/149‑1 (MQ=255)                                                                              cTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGc                                                                      <  3:89542/149‑1 (MQ=255)                                                                                 gTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCg                                                                    <  7:276442/148‑1 (MQ=255)                                                                                  tCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCATGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTa                                                                  <  5:148857/149‑1 (MQ=255)                                                                                    ggTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAg                                                                 <  2:161018/148‑1 (MQ=255)                                                                                     gTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCa                                                               >  7:293251/1‑149 (MQ=255)                                                                                     gTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGACGTAGc                                                                >  7:321209/1‑148 (MQ=255)                                                                                         cTTCGCGGACCACGTCGGTTTCGTAGCCCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTGCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATCGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAAca                                                            >  3:22144/1‑147 (MQ=255)                                                                                               ggACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGa                                                      >  3:320191/1‑148 (MQ=255)                                                                                                       tCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGc                                                                                                   >  3:224979/1‑95 (MQ=255)                                                                                                       tCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGc                                                                                                   <  4:224979/95‑1 (MQ=255)                                                                                                                tAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGcc                                    <  4:320191/149‑1 (MQ=255)                                                                                                                       gTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCGATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCg                             >  5:156369/1‑149 (MQ=255)                                                                                                                           gACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATGGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTa                                                                  >  3:247410/1‑108 (MQ=255)                                                                                                                           gACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATGGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTa                                                                  <  4:247410/108‑1 (MQ=255)                                                                                                                                gggCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTa                                                                  >  7:1145/1‑103 (MQ=255)                                                                                                                                gggCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTa                                                                  <  8:1145/103‑1 (MQ=255)                                                                                                                                 ggCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCt                   <  8:321209/149‑1 (MQ=255)                                                                                                                                  gCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCTg                  >  3:308867/1‑149 (MQ=255)                                                                                                                                   cAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCTgg                 <  8:87583/149‑1 (MQ=255)                                                                                                                                      tCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCTGGCAc              >  5:74335/1‑149 (MQ=255)                                                                                                                                        tGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCTGGCACtt            >  5:270754/1‑149 (MQ=255)                                                                                                                                        tGGAACGACGCCCTGGCTCCGTTCCAGAT‑‑TGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGtt                                 <  4:231812/126‑1 (MQ=37)                                                                                                                                        tGGAACGACGCCCTGGCTCCGTTCCAGAT‑‑TGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGtt                                 >  3:231812/1‑126 (MQ=37)                                                                                                                                             cGGAGCCAGGGCGTCGTTCAAGATGATGCCCTTGTCGTCATAGCACTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCTGGCACTTCAgcg       >  8:276736/1‑149 (MQ=255)                                                                                                                                                  ccAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGTACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCTGGCACTTCAGCGCCTCg  >  8:316016/1‑149 (MQ=255)                                                                                                                                                  ccAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCTGGCACTTCAGCGCCTCg  <  8:293251/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                  TTGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGGGCAATCTGGAACGGAGCCAGGGCGTCGTTCCAGATGATGCCCTTGTCGTCATAGCTCTGCTCGATGGCAGCTGCAACCACACGGGACACGCCAATGCCGTAGCAACCCATCGACAGTACGACCGGCTTGCCGTTCTCGCCCAGCACCTGGCACTTCAGCGCCTCG  >  NC_002947/1383478‑1383771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑