Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,390,996	G→C	7.4%	G268A (GGC→GCC)	aspS →	aspartate‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,390,996	0	G	C	7.4%	193.3 / 4.3	64	G268A (GGC→GCC)	aspS	aspartate‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (31/28);  new base C (3/2);  total (34/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

CCGTGACGAAGACCTGCGTGCCGACCGTCAGCCGGAATTCACCCAGATCGACATCGAGACCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGACTTCAAGGTGTTCGCCGGCCCGGCCAACGA  >  NC_002947/1390850‑1391140                                                                                                                                                   |                                                                                                                                                 ccGTGACGAAGACCTGCGTGCCGACCGTCAGCCGGAATTCACCCAGATCGACATCGAGACCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCgg                                                                                                                                                  <  1:70336/147‑1 (MQ=255) ccGTGACGAAGACCTGCGTGCCGACCGTCAGCCGGAATTCACCCAGATCGACATCGAGACCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGc                                                                                                                                                 >  8:135657/1‑148 (MQ=255) ccGTGACGAAGACCTGCGTGCCGACCGTCAGCCGGAATTCACCCAGATCGACATCGAGACCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGc                                                                                                                                                 <  8:110043/148‑1 (MQ=255) ccGTGACGAAGACCTGCGTGCCGACCGTCAGCCGGAATTCACCCAGATCGACATCGAGACCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGc                                                                                                                                                 <  3:275159/148‑1 (MQ=255)  cGTGACGAAGACCTGCGTGCCGACCGTCAGCCGGAATTCACCCAGATCGACATCGAGACCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCg                                                                                                                                                >  4:94873/1‑148 (MQ=255)             ccTGCGTGCCGACCGTCAGCCGGAATTCACCCAGATCGACATCGAGACCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGcaca                                                                                                                                    <  5:122960/149‑1 (MQ=255)                     ccGACCGTCAGCCGGAATTCACCCAGATCGACATCGAGACCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTc                                                                                                                            >  3:153527/1‑149 (MQ=255)                      cGACCGTCAGCCGGAATTCACCCAGATCGACATCGAGACCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGCCGAATTccaga                                                                                                                                       >  6:61047/1‑134 (MQ=255)                      cGACCGTCAGCCGGAATTCACCCAGATCGACATCGAGACCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGCCGAATTccaga                                                                                                                                       <  5:61047/137‑4 (MQ=255)                          cGTCAGCCGGAATTCACCCAGATCGACATCGAGACCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAaga                                                                                                                       <  7:135657/149‑1 (MQ=255)                           gTCAGCCGGAATTCACCCAGATCGACATCGAGACCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAagag                                                                                                                      >  1:73719/1‑149 (MQ=255)                           gTCAGCCGGAATTCACCCAGATCGACATCGAGACCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAagag                                                                                                                      <  3:94873/149‑1 (MQ=255)                            tCAGCCGGAATTCACCCAGATCGACATCGAGACCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAgg                                                                                                                     <  5:317080/149‑1 (MQ=255)                              aGCCGGAATTCACCCAGATCGACATCGAGACCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCGGGAATTCGCCGAATTccagat                                                                                                                                      >  4:36806/1‑126 (MQ=255)                              aGCCGGAATTCACCCAGATCGACATCGAGACCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCGGGAATTCGCCGAATTccagat                                                                                                                                      <  3:36806/130‑5 (MQ=255)                                ccGGAATTCACCCAGATCGACATCGAGACCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCAt                                                                                                                 <  1:159007/149‑1 (MQ=255)                                 cGGAATTCACCCAGATCGACATCGAGACCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATg                                                                                                                <  1:11309/149‑1 (MQ=255)                                        cACCCAGATCGACATCGAGACCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGcgccgc                                                                                                          >  6:174601/1‑148 (MQ=255)                                         agccAGATCCACATCGAGACCAGTTTCCTCGATGAAATCGAGAGCATGGCCCTGACCCAAAGAATGAACCGCCAGATGGTCAAGCAAGTGCTCGATCTGGAATTCGGCGAATCCCCGCACATGACCTTCGAAGAGGCCATCCGCCGCTa                                                                                                        >  8:300936/3‑149 (MQ=255)                                           ccAGATCGACATCGAGACCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACg                                                                                                      <  5:117069/149‑1 (MQ=255)                                                  gACATCGAGACCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGa                                                                                               >  8:67319/1‑149 (MQ=255)                                                  gACATCGAGACCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCAGGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGa                                                                                               >  8:62587/1‑149 (MQ=255)                                                   aCATCGAGACCAGTTTCCTCGATGAAAGGGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGa                                                                                               >  1:194909/1‑148 (MQ=255)                                                     aTCGAGACCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGAc                                                                                              >  5:120749/1‑147 (MQ=255)                                                         agaCCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGc                                                                                          >  6:335363/1‑147 (MQ=255)                                                             cAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGAcc                                                                                    >  3:330675/1‑149 (MQ=255)                                                               gTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTg                                                                                  >  2:165356/1‑149 (MQ=255)                                                                tttCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCtcc                                                                                 >  2:298346/1‑147 (MQ=255)                                                                tttCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGc                                                                                 <  6:120749/149‑1 (MQ=255)                                                                tttCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGc                                                                                 <  4:330675/149‑1 (MQ=255)                                                                   ccTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGt                                                                               <  1:298346/148‑1 (MQ=255)                                                                     tCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGCCACGCCAGGCCGGCggggt                                                                            >  2:323826/1‑145 (MQ=255)                                                                     tCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGc                                                                                 <  1:323826/144‑1 (MQ=255)                                                                       gATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTcc                                                                          >  3:331097/1‑149 (MQ=255)                                                                           aaaGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCTGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCt                                                                       <  5:261852/148‑1 (MQ=255)                                                                            aaGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCa                                                                                                                  >  4:41227/1‑104 (MQ=255)                                                                             aGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGa                                                                                      >  7:11786/1‑131 (MQ=255)                                                                             aGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGa                                                                                      <  8:11786/131‑1 (MQ=255)                                                                                  gaTCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCa                                                                                        <  3:147461/124‑1 (MQ=255)                                                                                  gaTCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCa                                                                                        >  4:147461/1‑124 (MQ=255)                                                                                   aTCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACaa                                                                                            <  6:100249/119‑1 (MQ=255)                                                                                   aTCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACaa                                                                                            >  5:100249/1‑119 (MQ=255)                                                                                     cATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCAGCACATGACCTTCGAAGAGGCAATGCGCAGCTACGGCTc                                                                                                  >  5:178539/1‑111 (MQ=255)                                                                                       tGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGAc                                                          <  5:117824/149‑1 (MQ=255)                                                                                                 cGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGAc                                                 >  6:304582/1‑148 (MQ=255)                                                                                                   aaaGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAg                                              <  4:153527/149‑1 (MQ=255)                                                                                                       cATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTg                                           >  3:298878/1‑148 (MQ=255)                                                                                                       cATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTg                                           >  7:90536/1‑148 (MQ=255)                                                                                                              cGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGt                                   <  1:165356/149‑1 (MQ=255)                                                                                                               gCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTc                                  >  3:235283/1‑149 (MQ=255)                                                                                                                 aaGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGa                                <  4:331097/149‑1 (MQ=255)                                                                                                                  aGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGAc                               >  3:87082/1‑149 (MQ=255)                                                                                                                     tGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGACtt                             >  4:155293/1‑148 (MQ=255)                                                                                                                       ttCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGACTTc                            >  3:21589/1‑147 (MQ=255)                                                                                                                         cAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGACTTCAAgg                        >  6:198301/1‑149 (MQ=255)                                                                                                                         cAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGACTTCAAg                         <  2:73719/148‑1 (MQ=255)                                                                                                                          aaGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGACTTCAAgg                        <  4:21589/148‑1 (MQ=255)                                                                                                                            ggAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGACTTCAAGgtgt                     <  4:87082/149‑1 (MQ=255)                                                                                                                              aaGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGACTTCAAGGTGTTc                   <  5:335363/149‑1 (MQ=255)                                                                                                                                 tGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGACTTCAAGGTGTTCGcc                >  3:146053/1‑149 (MQ=255)                                                                                                                                      gATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGACTTCAAg                         >  6:247455/1‑135 (MQ=255)                                                                                                                                      gATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGACTTCAAg                         <  5:247455/135‑1 (MQ=255)                                                                                                                                         cgggAATTCGCCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGACTTCAAGGTGTTCGccggcccggc        >  3:263061/3‑149 (MQ=255)                                                                                                                                            gAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGACTTCAAGGTGTTCGCCGGCCCGGCCaa     <  2:194909/149‑1 (MQ=255)                                                                                                                                               ttCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGACTTCAAGGTGTTGGCCGGGCCGGCGAAcac  <  5:304582/149‑3 (MQ=255)                                                                                                                                                   |                                                                                                                                                 CCGTGACGAAGACCTGCGTGCCGACCGTCAGCCGGAATTCACCCAGATCGACATCGAGACCAGTTTCCTCGATGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGACTTCAAGGTGTTCGCCGGCCCGGCCAACGA  >  NC_002947/1390850‑1391140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑