Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,392,039 G→A 6.3% intergenic (+70/‑45) aspS → / → PP_1214 aspartate‑‑tRNA ligase/transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,392,0390GA 6.3% 231.8 / 3.7 80intergenic (+70/‑45)aspS/PP_1214aspartate‑‑tRNA ligase/transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (34/40);  new base A (2/3);  total (37/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.93e-01

CCAGGCCCCTGGCATGGTCGACGCCAAGGCCCTGCGCGAACTGCACATCCGACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAAGAGAGGCAAGGTCTTCACCAAGTGGATCCGTGAACTGACGGTCGC  >  NC_002947/1391891‑1392187
                                                                                                                                                       |                                                                                                                                                    
ccAGGCCCCTGGCATGGTCGACGCCAAGGCCCTGCGCGAACTGCACATCCGACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCg                                                                                                                                                      >  2:15939/1‑149 (MQ=255)
  aGGCCCCTGGCATGGTCGACGCCAAGGCCCTGCGCGAACTGCACATCCGACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGt                                                                                                                                                     >  6:341661/1‑148 (MQ=255)
     ccccTGGCATGGTCGACGCCAAGGCCCTGCGCGAACTGCACATCCGACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATg                                                                                                                                                 >  5:250512/1‑149 (MQ=255)
         tGGCATGGTCGACGCCAAGGCCCTGCGCGAACTGCACATCCGACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATggggc                                                                                                                                             >  2:309022/1‑149 (MQ=255)
           gCATGGTCGACGCCAAGGCCCTGCGCGAACTGCACATCCGACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATggggcgg                                                                                                                                           <  4:48335/149‑1 (MQ=255)
           gCATGGTCGACGCCAAGGCCCTGCGCGAACTGCACATCCGACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATggggc                                                                                                                                             <  2:45796/147‑1 (MQ=255)
           gCATGGTCGACGCCAAGGCCCTGCGCGAACTGCACATCCGACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCATGACggggcgg                                                                                                                                           >  8:58406/1‑149 (MQ=255)
             aTGGTCGACGCCAAGGCCCTGCGCGAACTGCACATCCGACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATggggcgggg                                                                                                                                         <  7:308229/149‑1 (MQ=255)
                gTCGACGCCAAGGCCCTGCGCGAACTGCACATCCGACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATggggcgggacg                                                                                                                                       <  5:341661/148‑4 (MQ=255)
                gTCGACGCCAAGGCCCTGCGCGAACTGCACATCCGACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCg                                                                                                                                      >  8:82507/1‑149 (MQ=255)
                gTCGACGCCAAGGCCCTGCGCGAACTGCACATCCGACTGCGCGAACAGACAAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCATGACggggcgggacg                                                                                                                                       <  2:313872/148‑4 (MQ=255)
                   gACGCCAAGGCCCTGCGCGAACTGCACATCCGACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCTTCCCGCCCCGTCATGGGGCGGGGCCtttt                                                                                                                                   >  8:267076/1‑149 (MQ=255)
                   gACGCCAAGGCCCTGCGCGAACTGCACATCCGACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGttt                                                                                                                                   <  3:59334/149‑1 (MQ=255)
                   gACGCCAAGGCCCTGCGCGAACTGCACATCCGACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGCCCCGCCCCATGACggggcgggacggga                                                                                                                                    <  7:15763/148‑7 (MQ=255)
                          aGGCCCTGCGCGAACTGCACATCCGACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTc                                                                                                                            <  7:225968/149‑1 (MQ=255)
                             gccTGCGCGAACTGCACATCCGACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCaaa                                                                                                                         <  7:87228/148‑1 (MQ=255)
                             cccTGCGCGAACTGCACATCCGACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCaaa                                                                                                                         <  1:15939/149‑1 (MQ=255)
                              ccTGCGCGAACTGCACATCCGACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAg                                                                                                                        >  3:236456/1‑149 (MQ=255)
                               cTGCGCGAACTGCACATCCGACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATggggcggg                                                                                                                                          <  6:101974/130‑1 (MQ=255)
                               cTGCGCGAACTGCACATCCGACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATggggcggg                                                                                                                                          >  5:101974/1‑130 (MQ=255)
                               cTGCGCGAACTGCACATCCGACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAg                                                                                                                        >  8:34559/1‑148 (MQ=255)
                                  cgcgAACTGCACATCCGACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACATGAGGAGTGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGACATGGGGCGGGGCCGTTTGTGATTCAAAg                                                                                                                        >  1:107424/1‑148 (MQ=255)
                                   gcgAACTGCACATCCGACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTc                                                                                                                   <  4:88178/149‑1 (MQ=255)
                                    cgAACTGCTCATCCGACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCg                                                                                                                  <  5:200300/149‑1 (MQ=255)
                                           cacaTCCGACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCg                                                                                                            <  3:178230/148‑1 (MQ=255)
                                           cacaTCCGACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCg                                                                                                            <  8:163491/148‑1 (MQ=255)
                                           cacaTCCGACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGt                                                                                                           >  8:248679/1‑149 (MQ=255)
                                           cacaTCCGACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGt                                                                                                           >  8:186220/1‑149 (MQ=255)
                                           cacaTCCGACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGt                                                                                                           >  5:109201/1‑149 (MQ=255)
                                            acaTCCGACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGtt                                                                                                          <  7:248679/149‑1 (MQ=255)
                                                  gACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCt                                                                                                    <  4:204730/149‑1 (MQ=255)
                                                    cTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTgg                                                                                                  <  7:82507/149‑1 (MQ=255)
                                                       cgcgAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCa                                                                                               >  2:276290/1‑149 (MQ=255)
                                                        gcgAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCAt                                                                                              >  8:107938/1‑149 (MQ=255)
                                                         cgAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCAtt                                                                                             <  4:236456/149‑1 (MQ=255)
                                                             cAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTcc                                                                                           >  7:167431/1‑147 (MQ=255)
                                                             cAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCaa                                                                                         <  7:107938/149‑1 (MQ=255)
                                                              aGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTa                                                                                                         <  4:72307/132‑1 (MQ=255)
                                                              aGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTa                                                                                                         >  3:72307/1‑132 (MQ=255)
                                                               gACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGt                                                                                       <  2:107424/149‑1 (MQ=255)
                                                                aCCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGCCCCGCCCCATGACggggcgggacgg                                                                                                                                      <  4:317630/101‑5 (MQ=38)
                                                                aCCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGCCCCGCCCCATGACggggcgggacgg                                                                                                                                      >  3:317630/1‑97 (MQ=38)
                                                                 ccAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTgg                                                                                     <  7:186220/149‑1 (MQ=255)
                                                                  cAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTggg                                                                                    <  1:154159/149‑1 (MQ=255)
                                                                  cAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTggg                                                                                    <  1:309022/149‑1 (MQ=255)
                                                                       tCGAGTAAGCAGTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAAc                                                                               <  2:82317/149‑1 (MQ=255)
                                                                        cGAGTAAGCAGTCCCCGGGCGCATCAACAA‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCAGACCCGCCCACTAATGGGGAGGGGCCGTTTGTGAATCAAATGATTCGTAGTACTTATGGATGGAAATTACAAGTAGGCGAAc                                                                               >  2:309757/1‑148 (MQ=255)
                                                                          aGTAAGCAGTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATc                                                                            <  7:34559/149‑1 (MQ=255)
                                                                              aGCAGTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAg                                                                         >  7:227343/1‑148 (MQ=255)
                                                                               gCAGTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCa                                                                       >  1:260409/1‑149 (MQ=255)
                                                                               gCAGTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCa                                                                       >  1:323417/1‑149 (MQ=255)
                                                                               gCAGTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCa                                                                       <  2:323417/149‑1 (MQ=255)
                                                                                cAGTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCAc                                                                      <  6:250512/149‑1 (MQ=255)
                                                                                  gTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGGTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCg                                                                    >  3:22626/1‑149 (MQ=255)
                                                                                  acgcaaaggCGCATCCATGT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCAcc                                                                     <  5:188701/141‑1 (MQ=255)
                                                                                  acgcaaaggCGCATCCATGT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCAcc                                                                     >  6:188701/8‑148 (MQ=255)
                                                                                      ccGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCa                                                                  >  5:275743/1‑147 (MQ=255)
                                                                                                    aT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCaa                                                                          <  7:135561/125‑1 (MQ=255)
                                                                                                    aT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCaa                                                                          >  8:135561/1‑125 (MQ=255)
                                                                                                          a‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGcc                                                 >  6:315011/1‑146 (MQ=255)
                                                                                                          a‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGcc                                                 >  6:309838/1‑146 (MQ=255)
                                                                                                          a‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAag                                              <  1:276290/149‑1 (MQ=255)
                                                                                                            tGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCaa                                               >  7:122083/1‑147 (MQ=255)
                                                                                                            tGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAaga                                             <  8:122083/149‑1 (MQ=255)
                                                                                                                 cTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAAGAGAGGc                                        <  6:109201/149‑1 (MQ=255)
                                                                                                                    tGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAAGAGAGGCaa                                      <  6:275743/148‑1 (MQ=255)
                                                                                                                      cGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAAGAGAGGCAAGGt                                   >  7:88987/1‑149 (MQ=255)
                                                                                                                                cAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAAGAGAGGCAAGGTCTTCACCAAg                         <  8:88987/149‑1 (MQ=255)
                                                                                                                                 aGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAAGAGAGGCAAGGTCTTCACCAAGt                        <  2:260409/149‑1 (MQ=255)
                                                                                                                                 aGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAAGAGAGGCAAGGTCTTCACCAAGt                        >  3:294514/1‑149 (MQ=255)
                                                                                                                                  ggTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAAGAGAGGCAAGGTCTTCACCAAGTg                       <  3:303803/149‑1 (MQ=255)
                                                                                                                                    ttttttCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAAGAGAGGCAAGGTCTTCACCAAGTGGa                     >  1:54591/1‑149 (MQ=255)
                                                                                                                                       tttCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAAGAGAGGCAAGGTCTTCACCAAGTGGATc                   <  8:167431/148‑1 (MQ=255)
                                                                                                                                          cccgtcccgCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCTAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAAGAGAGGCAAGGTCTTCACCAAGTGGATCCGt                <  7:267076/148‑1 (MQ=255)
                                                                                                                                           ccgtcccgCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCAtt                                                                                             <  2:292197/70‑1 (MQ=255)
                                                                                                                                           ccgtcccgCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCAtt                                                                                             >  1:292197/1‑70 (MQ=255)
                                                                                                                                                  gCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGcc                                                 >  2:282654/1‑107 (MQ=255)
                                                                                                                                                  gCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAAGAGAGGCAAGGTCTTCACCAAGTGGATCCGTGAACTGACg       >  2:324657/1‑149 (MQ=255)
                                                                                                                                                  gCCCCGTCATGCGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGcc                                                 <  1:282654/107‑1 (MQ=255)
                                                                                                                                                     ccGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAAGAGAGGCAAGGTCTTCACCAAGTGGATCCGTGAACTGACGGTc    >  7:4275/1‑149 (MQ=255)
                                                                                                                                                      cGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAAGAGAGGCAAGGTCTTCACCAAGTGGATCCGTGAACTGACGGTCg   >  7:76677/1‑149 (MQ=255)
                                                                                                                                                       gTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAAGAGAGGCAAGGTCTTCACCAAGTGGATCCGTGAACTGACGGTCGc  <  4:294514/149‑1 (MQ=255)
                                                                                                                                                       gTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAAGAGAGGCAAGGTCTTCACCAAGTGGATCCGTGAACTGACGGTCGc  <  8:47101/149‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                                    
CCAGGCCCCTGGCATGGTCGACGCCAAGGCCCTGCGCGAACTGCACATCCGACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACAT‑‑GGA‑TGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAAGAGAGGCAAGGTCTTCACCAAGTGGATCCGTGAACTGACGGTCGC  >  NC_002947/1391891‑1392187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑