Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,424,253 G→T 8.2% A322S (GCT→TCT)  PP_5467 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,424,2530GT 8.2% 150.4 / 3.2 49A322S (GCT→TCT) PP_5467hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (22/23);  new base T (2/2);  total (24/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

AGTGCCCTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGC  >  NC_002947/1424109‑1424388
                                                                                                                                                |                                                                                                                                       
agTGCCCTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCttt                                                                                                                                     <  7:21657/149‑1 (MQ=255)
    cccTGAGCGCCAACCCGATAGGCGCGGACAGCCCGTACACCGGCCAGGCGGTGTTGGCAGAAGCGGTGAGCGGCTGCCCCCGCAAGGCCAAGGTCGAGACCTACTCGGTGACCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGc                                                                                                                                 <  8:325041/149‑1 (MQ=255)
     ccTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGc                                                                                                                                 >  4:327892/1‑148 (MQ=255)
         aGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGaa                                                                                                                              <  8:127974/147‑1 (MQ=255)
             cgATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACcag                                                                                                                        <  6:223903/149‑1 (MQ=255)
                  cTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCagcagc                                                                                                                    <  4:166385/148‑1 (MQ=255)
                   tGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGCCCAAGGTCGCGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCagcagc                                                                                                                    >  2:336175/1‑147 (MQ=255)
                            cgTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAg                                                                                                         <  8:109377/149‑1 (MQ=255)
                                 aGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCaaa                                                                                                    <  4:300194/149‑1 (MQ=255)
                                    ccGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGtt                                                                                                 >  8:28289/1‑149 (MQ=255)
                                     cGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTc                                                                                                <  1:171964/149‑1 (MQ=255)
                                           ccGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGCCTCTTTCAGCGat                                                                                                                              <  1:87907/113‑2 (MQ=255)
                                           ccGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGCCTCTTTCAGCGat                                                                                                                              >  2:87907/1‑112 (MQ=255)
                                                  ggCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGcc                                                                                   <  5:221482/149‑1 (MQ=255)
                                                   gCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCg                                                                                               <  4:220939/136‑1 (MQ=255)
                                                   gCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCg                                                                                               >  3:220939/1‑136 (MQ=255)
                                                           ggCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTg                                                                                     >  7:105163/1‑138 (MQ=255)
                                                           ggCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTg                                                                                     <  8:105163/138‑1 (MQ=255)
                                                           ggCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTg                                                                          >  1:171306/1‑149 (MQ=255)
                                                            gCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTg                                                                          <  5:137756/148‑1 (MQ=255)
                                                              tGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGc                                                                       >  4:250180/1‑149 (MQ=255)
                                                                      tGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCt                                                                                      >  3:5036/1‑126 (MQ=255)
                                                                      tGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCt                                                                                      <  4:5036/126‑1 (MQ=255)
                                                                                tGCCAGCAAGGACAAGGTCGATAACTACTCGGTGAGCTACAGCGGCTTCAAATGGCTGAAAGAGGCTTTCAGCGAATACCTGCAGCAAGTCCCCAAGGCATATTTGGCAGCCTTCTGAAAGGAGGTTGGGCTGTCGTCGTGGAGCAGt                                                      <  7:128223/148‑1 (MQ=255)
                                                                                   ccGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACt                                                  >  6:103729/1‑149 (MQ=255)
                                                                                    cGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCagga                                                                               >  1:288488/1‑119 (MQ=255)
                                                                                    cGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCagga                                                                               <  2:288488/119‑1 (MQ=255)
                                                                                    cGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACtt                                                 >  8:265971/1‑149 (MQ=255)
                                                                                            cAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGgc                                             >  7:298015/1‑145 (MQ=255)
                                                                                            cAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGgc                                             <  8:298015/145‑1 (MQ=255)
                                                                                                        ctactCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCgcc                                          <  8:201176/136‑1 (MQ=255)
                                                                                                        ctactCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCgcc                                          >  7:201176/1‑136 (MQ=255)
                                                                                                           ctCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCACGAGGTAGCGCTGTCGTCGTGGAGAAGTGACTTTGGCGCCAAGGCCAAGAACca                            >  8:171206/1‑147 (MQ=255)
                                                                                                                   gCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCg                  <  3:250180/149‑1 (MQ=255)
                                                                                                                    cTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCCCAGCGCCTCg                  >  2:146114/1‑148 (MQ=255)
                                                                                                                    cTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCg                  >  2:206171/1‑148 (MQ=255)
                                                                                                                    cTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCg                  >  7:54428/1‑148 (MQ=255)
                                                                                                                     tACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCgg                 >  3:101723/1‑148 (MQ=255)
                                                                                                                         gCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTgg                                                            <  6:343370/101‑1 (MQ=255)
                                                                                                                         gCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTgg                                                            >  5:343370/1‑101 (MQ=255)
                                                                                                                         gCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATgc            <  7:265971/149‑1 (MQ=255)
                                                                                                                            gCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGGCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGcc         <  1:146114/149‑1 (MQ=255)
                                                                                                                               tCAAATCGCTGAAAGAGGCTGTCAGCGAATCCCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATc      >  5:10682/1‑149 (MQ=255)
                                                                                                                                cAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCg     >  5:297618/1‑149 (MQ=255)
                                                                                                                                   aTCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGc  <  3:327892/149‑1 (MQ=255)
                                                                                                                                   aTCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGc  <  6:297618/149‑1 (MQ=255)
                                                                                                                                    tCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCggg   >  1:101895/1‑147 (MQ=255)
                                                                                                                                      gCTGAAAGCCTCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCgccaa                                        >  6:37398/1‑108 (MQ=255)
                                                                                                                                      gCTGAAAGCCTCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCgccaa                                        <  5:37398/108‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                       
AGTGCCCTGAGCGCGATGCTGATTGGCGCGTACAGCCCGTACACCGGCCAGGCGGTGTTGGCTGAAGCGGTGAGCGGCTTTGCCCGCAAGGCCAAGGTCGAGAACTACTCGGTGAGCTACAGCGGCTTCAAATCGCTGAAAGAGGCTTTCAGCGAATACCAGCAGCAAGTCCCCAAGGCAAAGTTCGCAGCCTTCTGCCAGGAGGTTGGGCTGTCGTCGTGGAGCAGTGACTTTGGCGCCAAGGCCAAGAACCACAGCGACTCGGAATGCGCCATCGGGC  >  NC_002947/1424109‑1424388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑