Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,429,512 G→A 9.8% intergenic (+29/‑451) mqo‑II → / → PP_1252 malate:quinone oxidoreductase/group II intron‑encoding maturase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,429,5120GA 9.8% 165.8 / 7.3 61intergenic (+29/‑451)mqo‑II/PP_1252malate:quinone oxidoreductase/group II intron‑encoding maturase
Reads supporting (aligned to +/- strand):  ref base G (27/28);  new base A (2/4);  total (29/32)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01

ACCGCTGAAGTGCTGCAACTGGGTAAACCGCCGGTGATCGACGCCAGCGTTGAATTCGGTGGTGCGGCAAGCAAGCCGGTCGAGAGCAAGCCTGAGAACGACATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGC‑TTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAGGCAAGG  >  NC_002947/1429370‑1429658
                                                                                                                                              |                                                                                                                                                   
acCGCTGAAGTGCTGCAACTGGGTAAACCGCCGGTGATCGACGCCAGCGTTGAATTCGGTGGTGCGGCAAGCAAGCCGGTCGAGAGCAAGCCTGAGAACGACATGGCGCTGTAATCGGCCATTGTTGTGAAAAAAAGCCACgaggtctc                                                                                                                                               >  4:267277/1‑145 (MQ=255)
  cGCTGAAGTGCTGCAACTGGGTAAACCGCCGGTGATCGACGCCAGCGTTGAATTCGGTGGTGCGGCAAGCAAGCCGGTCGAGAGCAAGCCTGAGAACGACATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGAcc                                                                                                                                             >  7:59266/1‑149 (MQ=255)
       aaGTGCTGCAACTGGGTAAACCGCCGGTGATCGACGCCAGCGTTGAATTCGGTGGTGCGGCAAGCAAGCCGGTCGAGAGCAAGCCTGAGAACGACATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGt                                                                                                                                         <  6:75049/148‑1 (MQ=255)
       aaGTGCTGCAACTGGGTAAACCGCCGGTGATCGACGCCAGCGTTGAATTCGGTGGTGCGGCAAGCAAGCCGGTCGAGAGCAAGCCTGAGAACGACATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTg                                                                                                                                        <  1:255679/149‑1 (MQ=255)
            ctgcAACTGGGTAAACCGCCGGTGATCGACGCCAGCGTTGAATTCGGTGGTGCGGCAAGCAAGCCGGTCGAGAGCAAGCCTGAGAACGACATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGC‑ttt                                                                                                                                  >  7:213291/1‑149 (MQ=255)
            ctgcAACTGGGTAAACCGCCGGTGATCGACGCCAGCGTTGAATTCGGTGGTGCGGCAAGCAAGCCGGTCGAGAGCAAGCCTGAGAACGACATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGAGGTCTCCCCCGTGGC‑tt                                                                                                                                   <  7:653/148‑1 (MQ=255)
             tgcAACTGGGTAAACCGCCGGTGATCGACGCCAGCGTTGAATTCGGTGGTGCGGCAAGCAAGCCGGTCGAGAGCAAGCCTGAGAACGACATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGC‑tttt                                                                                                                                 >  3:66055/1‑149 (MQ=255)
               cAACTGGGTAAACCGCCGGTGATCGACGCCAGCGTTGAATTCGGTGGTGCGGCAAGCAAGCCGGTCGAGAGCAAGCCTGAGAACGACATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGC‑tttttt                                                                                                                               >  6:324482/1‑149 (MQ=255)
               cAACTGGGTAAACCGCCGGTGATCGACGCCAGCGTTGAATTCGGTGGTGCGGCAAGCAAGCCGGTCGAGAGCAAGCCTGAGAACGACATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGC‑tttttt                                                                                                                               <  8:59266/149‑1 (MQ=255)
               cAACGGGGTAAACCGCCGGTGATCGACGCCAGCGTTGAATTCGGTGGTGCGGCAAGCAAGCCGGTCGAGAGCAAGCCTGAGAACGACATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGAGGTCTCCCCCGTGGC‑tttctt                                                                                                                               >  8:163353/1‑149 (MQ=255)
                 accgggTAAACCGCCGGTGATCGACGCCAGCGTTGAATTCGGTGGTGCGGCAAGCAAGCCGGTCGAGAGCAAGCCTGAGAACGACATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGAGGTCTCCCCCGTGGC‑tttctttc                                                                                                                             <  7:163353/146‑3 (MQ=255)
                 aCTGGGTAAACCGCCGGTGATCGACGCCAGCGTTGAATTCGGTGGTGCGGCAAGCAAGCCGGTCGAGAGCAAGCCTGAGAACGACATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGAGGTCTCCCCCGTGGC‑tttctttc                                                                                                                             <  2:17741/149‑3 (MQ=255)
                   tGGGTAAACCGCCGGTGATCGACGCCTGCGTTGAATTCGGTGGTGCGGCAAGCAAGCCGGTCGAGAGCATGCCTGAGAACGACATGGCGCTGTAATCTGCCATTGTTGTGAAAGAAATCCACGGGGGAGACCTCGTGGC‑TTTTTtgtgt                                                                                                                           <  3:73928/149‑1 (MQ=255)
                     ggTAAACCGCCGGTGATCGACGCCAGCGTTGAATTCGGTGGTGCGGCAAGCAAGCCGGTCGAGAGCAAGCCTGAGAACGACATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGC‑TTTTTtgt                                                                                                                             <  6:118859/145‑1 (MQ=255)
                            cgccgGTGATCGACGCCAGCGTTGAATTCGGTGGTGCGGCAAGCAAGCCGGTCGAGAGCAAGCCTGAGAACGACATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGC‑TTTTTTGTGTGCGCCAgg                                                                                                                   >  1:128363/1‑148 (MQ=255)
                            cgccgGTGATCGACGCCAGCGTTGAATTCGGTGGTGCGGCAAGCAAGCCGGTCGAGAGCAAGCCTGAGAACGACATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGC‑TTTTTTGTGTGCGCCAgg                                                                                                                   >  8:96871/1‑148 (MQ=255)
                                 gTGATCGACGCCAGCGTTGAATTCGGTGGTGCGGCAAGCAAGCCGGTCGAGAGCAAGCCTGAGAACGACATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGC‑TTTTTTGTGTGCGCCAGGCATGgc                                                                                                             <  1:216889/149‑1 (MQ=255)
                                 gTGATCGACGCCAGCGTTGAATTCGGTGGTGCGGCAAGCAAGCCGGTCGAGAGCAAGCCTGAGAACGACATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGC‑TTTTTTGTGTGCGCCAGGCATGgc                                                                                                             <  8:284827/149‑1 (MQ=255)
                                        aCGCCAGCGTTGAATTCGGTGGTGCGGCAAGCAAGCCGGTCGAGAGCAAGCCTGAGAACGACATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGC‑TTTTTTGTGTGCGCCAGGCATGGCGCGTTgc                                                                                                      >  4:120579/1‑149 (MQ=255)
                                          gCCAGCGTTGAATTCGGTGGTGCGGCAAGCAAGCCGGTCGAGAGCAAGCCTGAGAACGACATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGC‑TTTTTTGTGTGCGCCAGGCATGGCGCGTTgcgc                                                                                                    <  3:120579/149‑1 (MQ=255)
                                            cAGCGTTGAATTCGGTGGTGCGGCAAGCAAGCCGGTCGAGAGCAAGCCTGAGAACGACATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGC‑TTTTTTGTGTGCGCCAGGCATGGCGCGTTGcgcg                                                                                                   >  8:178342/1‑148 (MQ=255)
                                             aGCGTTGAATTCGGTGGTGCGGCAAGCAAGCCGGTCGAGAGCAAGCCTGAGAACGACATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGC‑TTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTa                                                                                                 <  5:324482/149‑1 (MQ=255)
                                               cGTTGAATTCGGTGGTGCGGCAAGCAAGCCGGTCGAGAGCAAGCCTGAGAACGACATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGC‑tttttt                                                                                                                               <  4:174444/117‑1 (MQ=255)
                                               cGTTGAATTCGGTGGTGCGGCAAGCAAGCCGGTCGAGAGCAAGCCTGAGAACGACATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGC‑tttttt                                                                                                                               >  3:174444/1‑117 (MQ=255)
                                               cGTTGAATTCGGTGGTGCGGCAAGCAAGCCGGTCGAGAGCAAGCCTGAGAACGACATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGAGGTCTCCCCCGTGGC‑tttctttc                                                                                                                             >  3:18096/1‑117 (MQ=38)
                                               cGTTGAATTCGGTGGTGCGGCAAGCAAGCCGGTCGAGAGCAAGCCTGAGAACGACATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGAGGTCTCCCCCGTGGC‑tttctttc                                                                                                                             <  4:18096/119‑3 (MQ=38)
                                                gTTGAATTCGGTGGTGCGGCAAGCAAGCCGGTCGAGAGCAAGCCTGAGAACGACATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGC‑TTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATgc                                                                                              <  2:177568/149‑1 (MQ=255)
                                                    aaTTCGGTGGTGCGGCAAGCAAGCCGGTCGAGAGCAAGCCTGAGAACGACATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGC‑TTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCaa                                                                                          >  6:120246/1‑149 (MQ=255)
                                                      ttCGGTGGTGCGGCAAGCAAGCCGGTCGAGAGCAAGCCTGAGAACGACATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGC‑TTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAAcc                                                                                        >  6:338499/1‑149 (MQ=255)
                                                       tCGGTGGTGCGGCAAGCAAGCCGGTCGAGAGCAAGCCTGAGAACGACATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGC‑TTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAAccc                                                                                       >  2:213289/1‑149 (MQ=255)
                                                                  gcaagcaaGGCGGTAGAGAGCATGCCTGAGAACGACATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGC‑TTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTtgtg                                                                            <  4:66055/149‑1 (MQ=255)
                                                                  gcaagcaaGCCGGTCGAGAGCAAGCCTGAGAACGACATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGC‑TTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTtgtg                                                                            <  4:288750/149‑1 (MQ=255)
                                                                    aagcaagcCGGTCGAGAGCAAGCCTGAGAACGACATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGC‑TTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTgtggt                                                                          >  6:340237/1‑149 (MQ=255)
                                                                     agcaagcCGGTCGAGAGCAAGCCTGAGAACGACATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGTGGAGACCTCGTGGC‑TTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGtt                                                                               <  8:42198/143‑1 (MQ=255)
                                                                     agcaagcCGGTCGAGAGCAAGCCTGAGAACGACATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGC‑TTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGtt                                                                               >  7:42198/1‑143 (MQ=255)
                                                                     agcaagcCGGTCGAGAGCAAGCCTGAGAACGACATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGC‑TTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTgtggtg                                                                         <  2:128363/149‑1 (MQ=255)
                                                                     agcaagcCGGTCGAGAGCAAGCCTGAGAACGACATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGC‑TTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTgtggtg                                                                         >  1:43702/1‑149 (MQ=255)
                                                                      gcaagcCGGTCGAGAGCAAGCCTGAGAACGACATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGC‑TATTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCCCTTGGTTGtggtgg                                                                        >  7:236608/1‑149 (MQ=255)
                                                                              gTCGAGAGCAAGCCTGAGAACGACATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGC‑TTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTgg                                                                <  7:96871/149‑1 (MQ=255)
                                                                               gcGAGAGCAAGCCTGAGATCGTCATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGC‑TTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTgg                                                                <  8:236608/147‑1 (MQ=255)
                                                                                       aaGCCTGAGAACGACATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGC‑TTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTgg                                                       <  1:213289/149‑1 (MQ=255)
                                                                                       aaGCCTGAGAACGACATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGC‑TTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTg                                                        >  3:29065/1‑148 (MQ=255)
                                                                                         gCCTGAGAACGACATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGC‑TTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAg                                                     <  7:178342/149‑1 (MQ=255)
                                                                                           cTGAGAAGGAGATGGCGGTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGC‑TTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAgg                                                    <  5:338499/148‑1 (MQ=255)
                                                                                            tGAGAACGACATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGC‑TTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTgg                                                                                 >  6:57774/1‑118 (MQ=255)
                                                                                            tGAGAACGACATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGC‑TTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTgg                                                                                 <  5:57774/118‑1 (MQ=255)
                                                                                             gagaACGACATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGC‑TTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTg                                                  >  6:217539/1‑148 (MQ=255)
                                                                                              agaACGACATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGCTTTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGt                                                   <  3:328321/147‑1 (MQ=255)
                                                                                               gaACGACATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGC‑TTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGaaa                                               <  5:217539/149‑1 (MQ=255)
                                                                                                 aCGACATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGC‑TTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGCCTTGGAGGTGAAAGt                                             >  8:65978/1‑149 (MQ=255)
                                                                                                     cATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGC‑TTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCctc                                         <  5:340237/149‑1 (MQ=255)
                                                                                                      aTGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGC‑TTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCctc                                         >  1:42373/1‑148 (MQ=255)
                                                                                                                      ccATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGC‑TTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAggg                        >  6:95076/1‑149 (MQ=255)
                                                                                                                              gtgAAAGAAAGCCACGGGGGAGACCTCGTGGC‑TTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCgg                              <  4:90678/135‑1 (MQ=255)
                                                                                                                              gtgAAAGAAAGCCACGGGGGAGACCTCGTGGC‑TTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCgg                              >  3:90678/1‑135 (MQ=255)
                                                                                                                               tgAAAGAAAGCCACGGGGGAGACCTCGTGGC‑TTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTa               <  5:95076/149‑1 (MQ=255)
                                                                                                                                gaaagaaaGCCACGGGGGAGACCTCGTGGC‑TTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAg              >  7:219443/1‑149 (MQ=255)
                                                                                                                                   agaaagCCACGGGGGAGACCTCGTGGC‑TTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCg           >  5:35800/1‑149 (MQ=255)
                                                                                                                                     aaagCCACGGGGGAGACCTCGTGGC‑TTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCg           >  5:268573/1‑147 (MQ=255)
                                                                                                                                     aaagCCACGGGGGAGACCTCGTGGC‑TTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGa         <  6:35800/149‑1 (MQ=255)
                                                                                                                                          cACGGGGGAGACCTCGTGGC‑TTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAGGc      >  6:240987/1‑147 (MQ=255)
                                                                                                                                            cGGGTGAGACCTCGTGGC‑TTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAGGCAAgg  <  8:213291/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                   
ACCGCTGAAGTGCTGCAACTGGGTAAACCGCCGGTGATCGACGCCAGCGTTGAATTCGGTGGTGCGGCAAGCAAGCCGGTCGAGAGCAAGCCTGAGAACGACATGGCGCTGTAATCGGCCATTGTTGTGAAAGAAAGCCACGGGGGAGACCTCGTGGC‑TTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAGGCAAGG  >  NC_002947/1429370‑1429658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑