Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,447,937 C→T 8.1% Q679* (CAG→TAG)  PP_1264 → fusaric acid resistance protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,447,9370CT 8.1% 139.4 / 2.3 49Q679* (CAG→TAG) PP_1264fusaric acid resistance protein
Reads supporting (aligned to +/- strand):  ref base C (25/20);  new base T (2/2);  total (27/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.42e-01

ACCCGGCCTATGCCGAGTCGCAGCCGTGGCGCCAGGCAATCCGTGTCATGGGCCGTGCGCTGACCCGGCTGTTCCTGCAGCCCAGCGCCAGCAACCACGAGCGCGCACTGGTGGCCGTGGACCACGCCATCGCCCGCGTGCAGGCCACCGACGAGCCATTCGCCCGGCACTTCGACACCTCGGTGCTGCGCCGCGTGCAAAGCTACCTGCACTTCATCCGTACTTCCCTGCTGGACCCACAGTCGCCACTGGCACCGGCGAAAGGATTGCACCATGCCCCGT  >  NC_002947/1447797‑1448078
                                                                                                                                            |                                                                                                                                             
aCCCGGCCTATGCCGAGTCGCAGCCGTGGCGCCAGGCAATCCGTGTCATGGGCCGTGCGCTGACCCGGCTGTTCCTGCAGCCCAGCGCCAGCAACCACGAGCGCGCACTGGTGGCCGTGGACCACGCCATCGCCCGCGTGCAGGCCAcc                                                                                                                                       >  4:328703/1‑149 (MQ=255)
  ccGGCCTATGCCGAGTCGCAGCCGTGGCGCCAGGCAATCCGTGTCATGGGCCGTGCGCTGACCCGGCTGTTCCTGCAGCCCAGCGCCAGCAACCACGAGCGCGCACTGGTGGCCGTGGACCACGCCATCGCCCGCGTGCAGGCCACcga                                                                                                                                     >  8:153910/1‑149 (MQ=255)
                  cGCAGCCGTGGCGCCAGGCAATCCGTGTCATGGGCCGTGCGCTGACCCGGCTGTTCCTGCAGCCCAGCGCCAGCAACCACGAGCGCGCACTGGTGGCCGTGGACCACGCCATCGCCCGCGTGCAGGCCACCGACGAGCCATTCGCCCgg                                                                                                                     >  8:36796/1‑149 (MQ=255)
                    cAGCCGTGGCGCCAGGCAATCCGTGTCATGGGCCGTGCGCTGACCCGGCTGTTCCTGCAGCCCAGCGCCAGCAACCACGAGCGCGCACTGGTGGCCGTGGACCACGCCATCGCCCGCGTGCAGGCCACCGACGAGCCATTCGcc                                                                                                                        >  1:201412/1‑144 (MQ=255)
                    cAGCCGTGGCGCCAGGCAATCCGTGTCATGGGCCGTGCGCTGACCCGGCTGTTCCTGCAGCCCAGCGCCAGCAACCACGAGCGCGCACTGGTGGCCGTGGACCACGCCATCGCCCGCGTGCAGGCCACCGACGAGCCATTCGcc                                                                                                                        <  2:201412/144‑1 (MQ=255)
                    cAGCCGTGGCGCCAGGCAATCCGTGTCATGGGCCGTGCGCTGACCCGGCTGTTCCTGCAGCCCAGCGCCAGCAACCACGAGCGCGCACTGGTGGCCGTGGACCACGCCATCGCCCGCGTGCAGGCCACCGACGAGCCATTCGCCCGGCa                                                                                                                   >  5:230796/1‑149 (MQ=255)
                     aGCCGTGGCGCCAGGCAATCCGTGTCATGGGCCGTGCGCTGACCCGGCTGTTCCTGCAGCCCAGCGCCAGCAACCACGAGCGCGCACTGGTGGCCGTGGACCACGCCATCGCCCGCGTGCAGGCCACCGACGAGCCATTCGCCCGGCAc                                                                                                                  >  7:188625/1‑149 (MQ=255)
                          tGGCGCCAGGCAATCCGTGTCATGGGCCGTGCGCTGACCCGGCTGTTCCTGCAGCCCAGCGCCAGCAACCACGAGCGCGCACTGGTGGCCGTGGACCACGCCATCGCCCGCGTGCAGGCCACCGACGAGTCATTCGCCCGGCACTTCGa                                                                                                             <  7:153910/149‑1 (MQ=255)
                          tGGCGCCAGGCAATCCGTGTCATGGGCCGTGCGCTGACCCGGCTGTTCCTGCAGCCCAGCGCCAGCAACCACGAGCGCGCACTGGTGGCCGTGGACCACGCCATCGCCCGCGTGCAGGCCACCGACGAGCCATTCGCCCGGCACTTCGa                                                                                                             <  6:230796/149‑1 (MQ=255)
                              gcCAGGCAATCCGTGTCATGGGCCGTGCGCTGACCCGGCTGTTCCTGCAGCCCAGCGCCAGCAACCACGAGCGCGCACTGGTGGCCGTGGACCACGCCATCGCCCGCGTGTAGGCCACCGACGAGCCATTCGCCCGGCACTTCGacac                                                                                                          >  1:311804/1‑148 (MQ=255)
                              gcCAGGCAATCCGTGTCATGGGCCGTGCGCTGACCCGGCTGTTCCTGCAGCCCAGCGCCAGCAACCACGAGCGCGCACTGGTGGCCGTGGACCACGCCATCGCCCGCGTGTAGGCCACCGACGAGCCATTCGCCCGGCACTTCGACAcc                                                                                                         >  1:316937/1‑149 (MQ=255)
                                 aGGCAATCCGTGTCATGGGCCGTGCGCTGACCCGGCTGTTCCTGCAGCCCAGCGCCAGCAACCACGAGCGCGCACTGGTGGCCGTGGACCACGCCATCGCCCGCGTGCAGGCCACCGACGAGCCATTCGCCCGGCACTTCGACACCTCg                                                                                                      <  8:297762/149‑1 (MQ=255)
                                       tCCGTGTCATGGGCCGTGCGCTGACCCGGCTGTTCCTGCAGCCCAGCGCCAGCAACCACGAGCGCGCACTGGTGGCCGTGGACCACGCCATCGCCCGCGTGCAGGCCACCGACGAGCCATTCGCCCGGCACTTCGACACCTCGGtgctg                                                                                                <  8:270392/149‑1 (MQ=255)
                                             tCATGGGCCGTGCGCTGACCCGGCTGTTCCTGCAGCCCAGCGCCAGCAACCACGAGCGCGCACTGGTGGCCGTGGACCACGCCATCGCCCGCGTGCAGGCCACCGACGAGCCATTCGCCCGGCACTTCGACACCTCGGTGCTGcgccgc                                                                                          <  8:188625/149‑1 (MQ=255)
                                              cATGGGCCGTGCGCTGACCCGGCTGTTCCTGCAGCCCAGCGCCAGCAACCACGAGCGCGCACTGGTGGCCGTGGACCACGCCATCGCCCGCGTGCAGGCCACCGACGAGCCATTCGCCCGGCACTTCGACACCTCGGTGCTGCGCcgcg                                                                                         >  6:225740/1‑149 (MQ=255)
                                                 gggCCGTGCGCTGACCCGGCTGTTCCTGCAGCCCAGCGCCAGCAACCACGAGCGCGCACTGGTGGCCGTGGACCACGCCATCGCCCGCGTGCAGGCCACCGACGAGCCATTCGCCCGGCACTTCGACACCTCGGTGCTGCGCCGCGTg                                                                                       >  3:280710/1‑148 (MQ=255)
                                                    ccGTGCGCTGACCCGGCTGTTCCTGCAGCCCAGCGCCAGCAACCACGAGCGCGCACTGGTGGCCGTGGACCACGCCATCGCCCGCGTGCAGGCCACCGACGAGCCATTCGCCCGGCACTTCGACACCTCGGTGCTGCGCCGCGTGCaaa                                                                                   <  8:66250/149‑1 (MQ=255)
                                                     cGTGCGCTGACCCGGCTGTTCCTGCAGCCCAGCGCCAGCAACCACGAGCGCGCACTGGTGGCCGTGGACCACGCCATCGCCCGCGTGCAGGCCACCGACGAGCCATTCGCCCGGCACTTCGACACCTCGGTGCTGCGCCGCGTGCAAAg                                                                                  >  3:96392/1‑149 (MQ=255)
                                                                ccGGCTGTTCCTGCAGCCCAGCGCCAGCAACCACGAGCGCGCACTGGTGGCCGTGGACCACGCCATCGCCCGCGTGCAg                                                                                                                                             <  1:339529/79‑1 (MQ=255)
                                                                ccGGCTGTTCCTGCAGCCCAGCGCCAGCAACCACGAGCGCGCACTGGTGGCCGTGGACCACGCCATCGCCCGCGTGCAg                                                                                                                                             >  2:339529/1‑79 (MQ=255)
                                                                ccGGCTGTTCCTGCAGCCCAGCGCCAGCAACCACGAGCGCGCACTGGTGGCCGTGGACCACGCCATCGCCCGCGTGCAGGCCACCGACGAGCCATTCGCCCGGCACTTCGACACCTCGGTGCTGCGCCGCGTGCaaa                                                                                   >  6:345948/1‑137 (MQ=255)
                                                                ccGGCTGTTCCTGCAGCCCAGCGCCAGCAACCACGAGCGCGCACTGGTGGCCGTGGACCACGCCATCGCCCGCGTGCAGGCCACCGACGAGCCATTCGCCCGGCACTTCGACACCTCGGTGCTGCGCCGCGTGCaaa                                                                                   <  5:345948/137‑1 (MQ=255)
                                                                ccGGCTGTTCCTGCAGCCCAGCGCCAGCAACCACGAGCGCGCACTGGTGGCCGTGGACCACGCCATCGCCCGCGTGCAGGCCACCGACGAGCCATTCGCCCGGCACTTCGACACCTCGGTGCTGCGCCGCGTGCAAAGCTACCTGCa                                                                         <  1:136012/147‑1 (MQ=255)
                                                                       ttCCTGCAGCCCAGCGCCAGCAACCACGAGCGCGCACTGGTGGCCGTGGACCACGCCATCGCCCGCGTGCAGGCCACCGACGAGCCATTCGCCCGGCACTTCGACACCTCGGTGCTGCGCCGCGTGCAAAGCTACCTGCACTTCATc                                                                  <  4:96392/147‑1 (MQ=255)
                                                                        tCCTGCCGCCCAGCGCCACCACCCACGAGCCCGCACTGGTGGCCGTGGACCACGCCATCGCCCGCGTGCAGGCCACCGACGAGCCAGTCGCCCGGCACTTCGACCCCGCGGCGCTGCCCCGCGTGCAAAGCTAc                                                                              <  6:173386/134‑1 (MQ=255)
                                                                        tCCTGCAGCCCAGCGCCAGCAACCACGAGCGCGCACTGGTGGCCGTGGACCACGCCATCGCCCGCGTGCAGGCCACCGACGAGCCATTCGCCCGGCACTTCGACACCTCGGTGCTGCGCCGCGTGCAAAGCTAc                                                                              >  5:173386/1‑134 (MQ=255)
                                                                             cAGCCCAGCGCCAGCAACCACGAGCGCGCACTGGTGGCCGTGGACCACGCCATCGCCCGCGTGTAGGCCACCGACGAGCCATTCGCCCGGCACTTCGACACCTCGGTGCTGCGCCGCGTGCAAAGCTACCTGCACTTCATCCGTACtt                                                           <  2:311804/148‑1 (MQ=255)
                                                                             cAGCCCAGCGCCAGCAACCACGAGCGCGCACTGGTGGCCGTGGACCACGCCATCGCCCGCGTGTAGGCCACCGACGAGCCATTCGCCCGGCACTTCGACACCTCGGTGCTGCGCCGCGTGCAAAGCTACCTGCACTTCATCCGTACtt                                                           <  2:316937/148‑1 (MQ=255)
                                                                                cccAGCGCCAGCAACCACGAGCGCGCACTGGTGGCCGTGGACCACGCCATCGCCCGCGTGCAGGCCACCGACGAGCCATTCGCCCGGCACTTCGACACCTCGGTGCTGCGCCGCGTGCAAAGCTACCTGCACTTCATCCGTACTTCCct                                                       >  4:69468/1‑149 (MQ=255)
                                                                                 ccAGCGCCAGCAACCACGAGCGCGCACTGGTGGCCGTGGACCACGCCATCGCCCGCGTGCAGGCCACCGACGAGCCATTCGCCCGGCACTTCGACACCTCGGTGCTGCGCCGCGTGCAAAGCTACCTGCACTTCATCCGTACTTcc                                                         >  8:289493/1‑146 (MQ=255)
                                                                                 ccAGCGCCAGCAACCACGAGCGCGCACTGGTGGCCGTGGACCACGCCATCGCCCGCGTGCAGGCCACCGACGAGCCATTCGCCCGGCACTTCGACACCTCGGTGCTGCGCCGCGTGCAAAGCTACCTGCACTTCATCCGTACTTcc                                                         <  7:289493/146‑1 (MQ=255)
                                                                                 ccAGCGCCAGCAACCACGAGCGCGCACTGGTGGCCGTGGACCACGCCATCGCCCGCGTGCAGGCCACCGACGAGCCATTCGCCCGGCACTTCGACACCTCGGTGCTGCGCCGCGTGCAAAGCTACCTGCACTTCATCCGTACTTCCctg                                                      >  1:302942/1‑149 (MQ=255)
                                                                                        cAGCAACCACGAGCGCGCACTGGTGGCCGTGGACCACGCCATCGCCCGCGTGCAGGCCACCGACGAGCCATTCGCCCGGCACTTCGACACCTCGGTGCTGCGCCGCGTGCAAAGCTACCTGCACTTCATCCGTACTTCCCTGCTGGAcc                                               >  5:114898/1‑149 (MQ=255)
                                                                                        cAGCAACCACGAGCGCGCACTGGTGGCCGTGGACCACGCCATCGCCCGCGTGCAGGCCACCGACGAGCCATTCGCCCGGCACTTCGACACCTCGGTGCTGCGCCGCGTGCAAAGCTACCTGCACTTCATCCGTACTTCCCTGCTGGAcc                                               >  8:260285/1‑149 (MQ=255)
                                                                                          gCAACCACGAGCGCGCACTGGTGGCCGTGGACCACGCCATCGCCCGCGTGCAGGCCACCGACGAGCCATTCGCCCGGCACTTCGACACCTCGGTGCTGCGCCGCGTGCAAAGCTACCTGCACTTCATCCGTACTTCCCTGCTGGACCca                                             >  7:142734/1‑149 (MQ=255)
                                                                                             aCCACGAGCGCGCACTGGTGGCCGTGGACCACGCCATCGCCCGCGTGCAGGCCACCGACGAGCCATTCGCCCGGCACTTCGACACCTCGGTGCTGCGCCGCGTGCAAAGCTACCTGCACTTCATCCGTACTTCCCTGCTGGACCCACAg                                          <  5:225740/149‑1 (MQ=255)
                                                                                              ccACGAGCGCGCACTGGTGGCCGTGGACCACGCCATCGCCCGCGTGCAGGCCACCGACGAGCCATTCGCCCGGCACTTCGACACCTCGGTGCTGCGCCGCGTGCAAAGCTACCTGCACTTCATCCGTACTTCCCTGCTGGACCCACAGt                                         >  1:59679/1‑149 (MQ=255)
                                                                                              ccACGAGCGCGCACTGGTGGCCGTGGACCACGCCATCGCCCGCGTGCAGGCCACCGACGAGCCATTCGCACGGCACTTCGACACCTCGGTGCTGCGCCGCGTGCAAAGCTACCTGCACTTCATCCGTACTTCCCTGCTGGACCCACAGt                                         >  1:279333/1‑149 (MQ=255)
                                                                                                 cGAGCGCGCACTGGTGGCCGTGGACCACGCCATCGCCCGCGTGCAGGCCACCGACGAGCCATTCGCCCGGCACTTCGACACCTCGGTGCTGCGCCGCGTGCAAAGCTACCTGCACTTCATCCGTACTTCCCTGCTGGACCCACAGTCGc                                      <  3:328703/149‑1 (MQ=255)
                                                                                                     cgcgcACTGGTGGCCGTGGACCACGCCATCGCCCGCGTGCAGGCCACCGACGAGCCATTCGCCCGGCACTTCGACACCTCGGTGCTGCGCCGCGTGCAAAGCTACCTGCACTTCATCCGTACTTCCCTGCTGGACCCACAGTCGCCACt                                  <  3:69468/149‑1 (MQ=255)
                                                                                                                  ccGTGGACCACGCCATCGCCCGCGTGCAGGCCACCGACGAGCCATTCGCCCGGCACTTCGACACCTCGGTGCTGCGCCGCGTGCAAAGCTACCTGCACTTCATCCGTACTTCCCTGCTGGACCCACAGTCGCCACTGGCACCGGCGaaa                     <  8:142734/149‑1 (MQ=255)
                                                                                                                   cGTGGACCACGCCATCGCCCGCGTGCAGGCCACCGACGAGCCATTCGCCCGGCACTTCGACACCTCGGTGCTGCGCCGCGTGCAAAGCTACCTGCACTTCATCCGTACTTCCCTGCTGGACCCACAGTCGCCACTGGCACCGGCGAAAg                    >  2:243372/1‑149 (MQ=255)
                                                                                                                   cGTGGACCACGCCATCGCCCGCGTGCAGGCCACCGACGAGCCATTCGCCCGGCACTTCGACACCTCGGTGCTGCGCCGCGTGCAAAGCTACCTGCACTTCATCCGTACTTCCCTGCTGGACCCACAGTCGCCACTGGCACCGGCGAAAg                    >  5:192717/1‑149 (MQ=255)
                                                                                                                          cACGCCATCGCCCGCGTGCAGGCCACCGACGAGCCATTCGCCCGGCACTTCGACACCTCGGTGCTGCGCCGCGTGCAAAGCTACCTGCACTTCATCCGTACTTCCCTGCTGGACCCACAGTCGCCACTGGCACCGGCGAAAGGATTGCa             <  7:36796/149‑1 (MQ=255)
                                                                                                                          cACGCCATCGCCCGCGTGCAGGCCACCGACGAGCCATTCGCCCGGCACTTCGACACCTCGGTGCTGCGCCGCGTGCAAAGCTACCTGCACTTCATCCGTACTTCCCTGCTGGACCCACAGTCGCCACTGGCACCGGCGAAAGGATTGCa             <  2:59679/149‑1 (MQ=255)
                                                                                                                          cACGCCATCGCCCGCGTGCAGGCCACCGACGAGCCATTCGCCCGGCACTTCGACACCTCGGTGCTGCGCCGCGTGCAAAGCTACCTGCACTTCATCCGTACTTCCCTGCTGGACCCACAGTCGCCACTGGCACCGGCGAAAGGATTGCa             >  2:86174/1‑149 (MQ=255)
                                                                                                                                     ccGCGTGCAGGCCACCGACGAGCCATTCGCCCGGCACTTCGCCACCTCGGTGCTGCGCCGCGTGCAAAGCTACCTGCACTTCATCCGTACTTCCCTGCTGGACCCACAGTCGCCACTGGCACCGGCGAAAGGATTGCACCATGCCCCGt  >  5:335427/1‑149 (MQ=255)
                                                                                                                                     ccGCGTGCAGGCCACCGACGAGCCATTCGCCCGGCACTTCGACACCTCGGTGCTGCGCCGCGTGCAAAGCTACCTGCACTTCATCCGTACTTCCCTGCTGGACCCACAGTCGCCACTGGCTCCGGCGaaa                     <  2:214840/130‑1 (MQ=255)
                                                                                                                                     ccGCGTGCAGGCCACCGACGAGCCATTCGCCCGGCACTTCGACACCTCGGTGCTGCGCCGCGTGCAAAGCTACCTGCACTTCATCCGTACTTCCCTGCTGGACCCACAGTCGCCACTGGCTCCGGCGaaa                     >  1:214840/1‑130 (MQ=255)
                                                                                                                                            |                                                                                                                                             
ACCCGGCCTATGCCGAGTCGCAGCCGTGGCGCCAGGCAATCCGTGTCATGGGCCGTGCGCTGACCCGGCTGTTCCTGCAGCCCAGCGCCAGCAACCACGAGCGCGCACTGGTGGCCGTGGACCACGCCATCGCCCGCGTGCAGGCCACCGACGAGCCATTCGCCCGGCACTTCGACACCTCGGTGCTGCGCCGCGTGCAAAGCTACCTGCACTTCATCCGTACTTCCCTGCTGGACCCACAGTCGCCACTGGCACCGGCGAAAGGATTGCACCATGCCCCGT  >  NC_002947/1447797‑1448078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑