Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,449,140	G→A	7.1%	*287* (TGA→TAA)	PP_1266 →	fusaric acid resistance protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,449,140	0	G	A	7.1%	162.4 / 2.5	56	*287* (TGA→TAA)	PP_1266	fusaric acid resistance protein
Reads supporting (aligned to +/- strand):  ref base G (26/26);  new base A (2/2);  total (28/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.74e-01

GAGCTGATCGCCGACGTGAACCCGACCTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTG‑GCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCAT‑CTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTGGGTTGCCGCTATGCAACATCTTTTG  >  NC_002947/1448992‑1449285                                                                                                                                                      |                                                                                                                                                   gAGCTGATCGCCGACGTGAACCCGACCTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTG‑GCGAAGGTTg                                                                                                                                                    <  2:87621/149‑1 (MQ=255) gAGCTGATCGCCGACGTGAACCCGACCTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTG‑GCGAAGGTTg                                                                                                                                                    <  2:129102/149‑1 (MQ=255)   gCTGATCGCCGACGTGAACCCGACCTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTG‑GCGAAGGTTGAc                                                                                                                                                  <  3:133461/149‑1 (MQ=255)   gCTGATCGCCGACGTGAACCCGACCTTCAACTGGCTGCGGCTGGCACAGCGGGGGACGTTGCGCATTCACATCGAAGAAGTGCCGGAAGGGTGTTGGCTGGCGGCGGGCACGACATGTACGGTGGAATTCAAGCCTG‑GCGAAGGTAGa                                                                                                                                                   >  8:128745/1‑148 (MQ=255)        tCGCCGACGTGAACCCGACCTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTG‑GCGAAGGTTGACCTTcg                                                                                                                                             >  3:178491/1‑149 (MQ=255)           ccGACGTGAACCCGACCTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTG‑GCGAAGGTTGACCTTCGCGa                                                                                                                                          >  1:300688/1‑149 (MQ=255)              aCGTGAACCCGACCTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTG‑GCGAAGGTTGACCTTCGCGACCa                                                                                                                                       >  2:111351/1‑149 (MQ=255)                    agccGACCTTCAACTGGGTGCGGCTGGCTGAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGGGGCGGGGGCGACGTGTACGGTGGTAGTCAAGCCTG‑GCGAAGGTTGACCTTCGCGACCATCCCgg                                                                                                                                 <  8:161537/147‑1 (MQ=255)                    aCCCGACCTTCAACTGTGTGCGGCTGGCACAGCGTGTGCCGTTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTG‑GCGAAGGTTGACCTTCGCGACCATCCCgg                                                                                                                                 <  6:188682/149‑1 (MQ=255)                       cGACCTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTG‑GCGAAGGTTGACCTTCGCGACCATCCCGGCCt                                                                                                                              >  4:252388/1‑149 (MQ=255)                        gACCTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTG‑GCGAAGGTTGACCTTCGCGACCATCCCGGCCTc                                                                                                                             >  5:262803/1‑149 (MQ=255)                         aCCTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTG‑GCGAAGGTTGACCTTCGCGACCATCCCGGCCTcc                                                                                                                            <  3:143456/149‑1 (MQ=255)                            ttCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTG‑GCGAAGGTTGACCTTcgc                                                                                                                                            >  6:280691/1‑130 (MQ=255)                            ttCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTG‑GCGAAGGTTGACCTTcgc                                                                                                                                            <  5:280691/130‑1 (MQ=255)                                aCTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTG‑GCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGcatc                                                                                                                      <  5:11129/148‑1 (MQ=255)                                aCTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTG‑GCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGcatc                                                                                                                      >  6:11129/1‑148 (MQ=255)                                    ggTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTG‑GCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCAT‑Ct                                                                                                                 <  5:203837/148‑1 (MQ=255)                                     gTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTG‑GCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGcatc                                                                                                                      <  4:15188/143‑1 (MQ=255)                                     gTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTG‑GCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGcatc                                                                                                                      >  3:15188/1‑143 (MQ=255)                                         ggCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTG‑GCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCAT‑CTGAGGGa                                                                                                           <  2:26355/149‑1 (MQ=255)                                                acaGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTG‑GCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCAT‑CTGTGGGAGCCctgc                                                                                                    >  7:229912/1‑149 (MQ=255)                                                acaGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTG‑GCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCAT‑CTGTGGGAGCCctgc                                                                                                    >  2:70501/1‑149 (MQ=255)                                                 caGCGGGTGCCGGTGCGCTTTCTCATCGATGAAGTGCCGGAAGGGTTTTTGTTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTG‑GGGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCTTCAT‑CTGTGGGAGCCctgct                                                                                                   <  3:151177/149‑1 (MQ=255)                                                 caGCGGGTGCCGGTGCGCATTCACTTCGATGAAGTGACGGAAGGGTTTTTGCTGTCGGCGGGGTCTTCGTGTACGTTGGTAGTCAAGCCTG‑TCGAAGGTTGACCTTCGCGACCATCCCGGCGTCCCGCATCAT‑CTGTGGGAGCCctgct                                                                                                   <  2:100913/149‑1 (MQ=255)                                                    cGGGTGCCGTTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGGAGTCAAGCCTT‑GCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCAT‑CTGTGGGAGCCCTGCTGat                                                                                                <  7:128745/149‑1 (MQ=255)                                                     gggTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTG‑GCGAAGGTTGACCTTCGCGACCATCCTGGCCTCCCGCATCAT‑CTGTGGGAGCCCTGCTGatg                                                                                               <  5:8865/149‑1 (MQ=255)                                                        tGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTG‑GCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCAT‑CTGTGGGAGCCCTGCTGatgcat                                                                                            <  2:300688/149‑1 (MQ=255)                                                          ccGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTG‑GCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCAT‑CTGTGGGAGCCCTGCTGatgcatgc                                                                                          <  4:178491/149‑1 (MQ=255)                                                                       acaTCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTG‑GCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCAT‑CTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTc                                                                             >  2:27409/1‑149 (MQ=255)                                                                            gATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTG‑GCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCAT‑CTGTGGGAGCCCTGCTGGTGCATGCGTATTAGCCAGTCGGCt                                                                         <  5:237028/148‑1 (MQ=255)                                                                             aTGAAGTGCCGGAAGGGTTTTTGCAGGCGGCGGGGACGACGTGTACGGTCGAAGTCAAGCATG‑GCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCAT‑CAGTGGGAGCCCTCCTGCTGCAGGCGTATTAGCCCGTCGGCTc                                                                        >  4:112274/1‑148 (MQ=255)                                                                                      cGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTG‑GCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCAT‑Ctgtg                                                                                                              >  8:120366/1‑101 (MQ=255)                                                                                      cGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTG‑GCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCAT‑Ctgtg                                                                                                              <  7:120366/101‑1 (MQ=255)                                                                                        gAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTG‑GCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCAT‑CTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAg                                                            >  4:331935/1‑149 (MQ=255)                                                                                                           cggGGACGACGTGTACGGTGGTAGTCAAGCCTG‑GCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCAT‑CTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTc                                         <  8:229912/149‑1 (MQ=255)                                                                                                            ggggACGACGTGTACGGTGGTAGTCAAGCCTG‑GCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCAT‑CTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTcc                                        <  6:262803/149‑1 (MQ=255)                                                                                                              ggACGACGTGTACGGTGGTAGTCAAGCCTG‑GCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCAT‑CTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCa                                      <  1:70501/149‑1 (MQ=255)                                                                                                               gacgacGTGTACGGTGGTAGTCAAGCCTG‑GCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCAT‑CTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCaa                                     >  5:194037/1‑149 (MQ=255)                                                                                                                  gacgTGTACGGTGGTAGTCAAGCCTGAGCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCAT‑CTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATc                                   >  1:178379/1‑149 (MQ=255)                                                                                                                     gtgtACGGTGGTAGTCAAGCCTG‑GCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCATACTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTc                                >  1:276051/1‑149 (MQ=255)                                                                                                                      tgtACGGTGGTAGTCAAGCCTG‑GCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCAT‑CTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCg                              <  5:323848/149‑1 (MQ=255)                                                                                                                        tACGGTGGTAGTCAAGCCTG‑GCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCAT‑CTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTTCCAATCGTCCGGt                            <  3:252388/149‑1 (MQ=255)                                                                                                                               gtAGTCAAGCCTG‑GCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCAT‑CTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCGATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTGGGTTGc                     >  2:249539/1‑149 (MQ=255)                                                                                                                                  gTCAAGCCTG‑GCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCAT‑CTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTGGGTTGCCg                   <  1:27409/148‑1 (MQ=255)                                                                                                                                   tCAAGCCTG‑GCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCAT‑CTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAACCGTCCGGTGGGTTGCCGc                  >  1:277319/1‑148 (MQ=255)                                                                                                                                     aaGCCTG‑GCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCAT‑CTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTGGGTTGCCGCTAt               >  3:285351/1‑149 (MQ=255)                                                                                                                                       gCCTG‑GCGAAGGTTGACCTTCGCGACCCTCCCGGCCTCCCGCATCAT‑CTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCACCTGGTCGCAATCGTCCGGTGGGTTGACGCTATg              >  6:249666/1‑148 (MQ=255)                                                                                                                                       gCCTG‑GCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCAT‑CTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTGGGTTGCCGCTATg              >  5:269643/1‑148 (MQ=255)                                                                                                                                       gCCTG‑GCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCAT‑CTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTGGGTTGCCGCTATGc             <  4:285351/149‑1 (MQ=255)                                                                                                                                         ggtcgCGAAGGTCAACCTTCGCGACCATCCCGGCCTCCCGCATCAT‑CTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTGGGTTg                      >  4:159699/5‑139 (MQ=255)                                                                                                                                         ggtcgCGAAGGTCAACCTTCGCGACCATCCCGGCCTCCCGCATCAT‑CTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTGGGTTg                      <  3:159699/135‑1 (MQ=255)                                                                                                                                            cgCGAAGGTCAACCTTCGCGACCATCCCGGCCTCCCGCATCAT‑CTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTGGGTTGCCg                   >  2:267034/2‑139 (MQ=255)                                                                                                                                            cgCGAAGGTCAACCTTCGCGACCATCCCGGCCTCCCGCATCAT‑CTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGATCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTGGGTTGCCg                   <  1:267034/138‑1 (MQ=255)                                                                                                                                             gCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCAT‑CTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTGGGTTGCCGCTATGCAACAt        >  4:320258/1‑149 (MQ=255)                                                                                                                                             gCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCAT‑CTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTGGGTTGCCGCTATGCAACAt        <  2:276051/149‑1 (MQ=255)                                                                                                                                               gAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCAT‑CTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTACGGTGGGTTGCCGCTATGCAACATCt      >  5:345495/1‑149 (MQ=255)                                                                                                                                                  ggTTGACCTTCGCGACCATCCCGGCCTCCCGCATCAT‑CTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTGGGTTGCCGCTATGCAACATCtttt   >  1:59572/1‑149 (MQ=255)                                                                                                                                                   gTTGACCTTCGCGACCATCCCGGCCTCCCGCATCAT‑CTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTGGGTTGCCGCTATGCAACATCTTTTg  <  6:345495/149‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                   GAGCTGATCGCCGACGTGAACCCGACCTTCAACTGGGTGCGGCTGGCACAGCGGGTGCCGGTGCGCATTCACATCGATGAAGTGCCGGAAGGGTTTTTGCTGGCGGCGGGGACGACGTGTACGGTGGTAGTCAAGCCTG‑GCGAAGGTTGACCTTCGCGACCATCCCGGCCTCCCGCATCAT‑CTGTGGGAGCCCTGCTGATGCATGCGTATTAGCCAGTCGGCTCAATCTGCGGCAGCCTCTTGCAGCAACTGGTCCCAATCGTCCGGTGGGTTGCCGCTATGCAACATCTTTTG  >  NC_002947/1448992‑1449285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑