Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,458,119 A→T 13.6% intergenic (+19/+21) PP_1276 → / ← algA hypothetical protein/mannose‑6‑phosphate isomerase/mannose‑1‑phosphate guanylyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,458,1190AT13.6% 119.6 / 9.4 50intergenic (+19/+21)PP_1276/algAhypothetical protein/mannose‑6‑phosphate isomerase/mannose‑1‑phosphate guanylyltransferase
Reads supporting (aligned to +/- strand):  ref base A (24/19);  new base T (4/3);  total (28/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

TCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGAAGCGCTCGATGTCATCCTCGCCCAGGTAGCTCCCGGACTGCACCTCGATGATCTC  >  NC_002947/1457973‑1458265
                                                                                                                                                  |                                                                                                                                                  
tCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGc                                                                                                                                                  >  2:263183/1‑149 (MQ=255)
tCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGc                                                                                                                                                  >  3:26262/1‑149 (MQ=255)
  gAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGcc                                                                                                                                                 >  7:289573/1‑148 (MQ=255)
      tGACTGCCACAACCATCAGGGCCGAAGCCTTCGCATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGGGGGTGGCCCATTCGACATCTACGTCATAGCCCCGCGCGTTGAATAAGCAGGCTCGCCCCTGGGCTGACCAggg                                                                                                                                            <  4:309931/149‑1 (MQ=255)
             cacaAGCATCAGGGCTGAATCCTTAGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTTGTGGGTGGCCAATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGACTCGCCCCTTGGCTGACCAGGGGCGAgcc                                                                                                                                     <  4:145172/149‑3 (MQ=255)
              acaAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGa                                                                                                                                        >  8:214591/1‑145 (MQ=255)
              acaAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCCCCCCTGGTCAGCCCAGGGGCGa                                                                                                                                        <  7:214591/145‑1 (MQ=255)
                     tCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGACTCGCCCCTGGGCTGACCAGGGGCGAgcct                                                                                                                                    >  4:192657/1‑139 (MQ=255)
                     tCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGACTCGCCCCTGGGCTGACCAGGGGCGAgcct                                                                                                                                    <  3:192657/142‑4 (MQ=255)
                       aGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATCTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACt                                                                                                                           <  5:327151/149‑1 (MQ=255)
                                  cTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGt                                                                                                                 <  1:126171/148‑1 (MQ=255)
                                  cTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTc                                                                                                                <  8:95364/149‑1 (MQ=255)
                                   ttCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCt                                                                                                               >  4:339240/1‑149 (MQ=255)
                                   ttCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGGCTGACCAgggg                                                                                                                                           >  4:148249/1‑121 (MQ=255)
                                   gtCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGACATAGCCCCGAGAGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCAGTTACTGCGACATGGTCt                                                                                                               >  6:23217/2‑149 (MQ=255)
                                             aaGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGaaa                                                                                                      <  4:26262/148‑1 (MQ=255)
                                                gggAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAAcgcc                                                                                                  >  1:48642/1‑149 (MQ=255)
                                                              cAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATgggg                                                                                     <  8:289573/148‑1 (MQ=255)
                                                               aGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGt                                                                                   >  5:118063/1‑149 (MQ=255)
                                                                        tCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTgc                                                                           <  6:118063/148‑1 (MQ=255)
                                                                           gggTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGccc                                                                       <  2:48642/149‑1 (MQ=255)
                                                                            ggtggtGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCa                                                                      >  2:80572/1‑149 (MQ=255)
                                                                                          cgaccctcATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGaa                                                        <  2:54171/142‑1 (MQ=255)
                                                                                          cATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCg                                                          >  7:253526/1‑147 (MQ=255)
                                                                                                aCATCTACGTCTTAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAAGGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGAAGCGCTc                                                  <  5:23217/149‑1 (MQ=255)
                                                                                                       cGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGAAGCGCTCGATGTCa                                           <  5:304997/149‑1 (MQ=255)
                                                                                                         tCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGAAGCGCTCGAt                                               <  1:12504/143‑1 (MQ=255)
                                                                                                         tCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGAAGCGCTCGAt                                               >  2:12504/1‑143 (MQ=255)
                                                                                                          cATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTAAGCCCAGGGGCGAGTCAGCATTTACAGCGAGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGAAGCGCTCGAt                                               >  1:236295/1‑142 (MQ=255)
                                                                                                           aTAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGAAGCGCTCGAt                                               >  4:97162/1‑141 (MQ=255)
                                                                                                           aTAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGAAGCGCTCGAt                                               <  3:97162/141‑1 (MQ=255)
                                                                                                               ccccGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGAAGCGCTCGATGTCATCCTCGcc                                   >  2:183589/1‑149 (MQ=255)
                                                                                                                  cGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGAAGCGCTCGATGTCATCCTCGCCCAg                                <  8:253526/149‑1 (MQ=255)
                                                                                                                   gAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGAGCGATGGTCTTCACCCAAACGCCGCGCTCGAAGGGGGAGGACGTGCGCCCATACACAACCTCGAACCGCTCGATGTCATCCTCGCCCacg                               >  8:191000/1‑147 (MQ=255)
                                                                                                                    aGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGAAGCGCTCGATGTCATCCTCGCCCAgg                               >  2:188962/1‑148 (MQ=255)
                                                                                                                     gCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATgggg                                                                                     >  6:180710/1‑93 (MQ=255)
                                                                                                                     gCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGAAGCGCTCGATGTCATCCTCGCCCAGGTa                             <  3:339240/149‑1 (MQ=255)
                                                                                                                       gTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCATCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGAAGCGCTCGATGTCATCCTCGCCCAGGTAGc                           >  2:214975/1‑149 (MQ=255)
                                                                                                                           aaTAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGAAGCGCTCGATGTCATCCTCGCCCAGGTAGCTccc                       >  6:320685/1‑149 (MQ=255)
                                                                                                                             tAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCAACGAAACGCCGCGCTCGATGGGGGTGGACGAGCGCCCATACACATCCTCGAAGAGATCGAAGTCATCCTCGACCAGCTAGCTCCCg                      >  4:176400/1‑148 (MQ=255)
                                                                                                                                  tgacTCGCCCCTGGGCTGACCAGGGGCGAGCCTGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGAAGCGCTCGATGTCATCCTCGCCCAGGTAGCTCCCGGACTg                 >  3:431/4‑148 (MQ=39)
                                                                                                                                      tCGCCCCTGGGCTGACCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGAAGCGCTCGATGTCATCCTCGCCCAGGTAGCTCCCGGACTGCACCt            >  5:341799/1‑149 (MQ=255)
                                                                                                                                       cGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGAAGCGCTCGATGTCATCCTCGCCCAGGTAGCTCCCGGACTGCACCTc           >  6:225206/1‑149 (MQ=255)
                                                                                                                                         ccccTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGGGCTCGATGGGGGTGGACGTGCGCGCATACACATCCTCGAAGCGCTCGATGTCATCCTCGCCCAGGTAGCTCCCGGACTGCACCTCga         <  7:191000/149‑1 (MQ=255)
                                                                                                                                          cccTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGaa                                                        <  4:26708/101‑1 (MQ=255)
                                                                                                                                          cccTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGaa                                                        >  3:26708/1‑101 (MQ=255)
                                                                                                                                          cccTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGAAGCGCTCGATGTCATCCTCGCCCAGGTAGCTCCCGGACTGCACCTCgat        >  1:327935/1‑149 (MQ=255)
                                                                                                                                          cccTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGAAGCGCTCGATGTCATCCTCGCCCAGGTAGCTCCCGGACTGCACCTCgat        <  5:320685/149‑1 (MQ=255)
                                                                                                                                               gTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGAAGCGCTCGATGTCATCCTCGCCCAGGTAGCTCCCGGACTGCACCTCGATGAtc    <  5:225206/148‑1 (MQ=255)
                                                                                                                                                tCAGCCCAGCGCCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGAAGCGCTCGATGTCATCCTCGCCCAGGTAGCTCCCGGACTGCACATCGATGAtctc  >  2:272253/1‑149 (MQ=255)
                                                                                                                                                  aGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGAAGCGCTCGATGTCATCCTCGCCCAGGTAGCTCCCGGACTGCACCTCGATGAtctc  >  8:74263/1‑147 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTCGACATCTACGTCATAGCCCCGAGCGTTGAATAAGCAGGCTCGCCCCTGGTCAGCCCAGGGGCGAGTCAGCATTTACTGCGCGATGGTCTTCACCGAAACGCCGCGCTCGATGGGGGTGGACGTGCGCCCATACACATCCTCGAAGCGCTCGATGTCATCCTCGCCCAGGTAGCTCCCGGACTGCACCTCGATGATCTC  >  NC_002947/1457973‑1458265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑