Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,469,808 A→G 8.0% V112A (GTG→GCG)  algK ← alginate biosynthesis protein AlgK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,469,8080AG 8.0% 137.5 / 3.1 51V112A (GTG→GCG) algKalginate biosynthesis protein AlgK
Reads supporting (aligned to +/- strand):  ref base A (25/21);  new base G (2/2);  total (28/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01

CTGCGCGTTGACCTTGGGGAAACTCTGCGGGTAGCTGAGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGACTTGCGCTTCGCTGTAGCCCAGGT  >  NC_002947/1469660‑1469955
                                                                                                                                                    |                                                                                                                                                   
ctgcGCGTTGACCTTGGGGAAACTCTCCGGGTAGCTGAGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGcca                                                                                                                                                     >  2:231398/1‑149 (MQ=255)
           ccTTGGGGAAACTCTCCGGGTAGCTGAGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAggcgg                                                                                                                                          <  1:231398/149‑1 (MQ=255)
            ctgggggAAACTCTGCGGGTAGCTGAGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAggcggc                                                                                                                                         <  7:104465/146‑1 (MQ=255)
            cTTGGGGAAACTCTGCGGGTAGCTGAGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAggcggc                                                                                                                                         <  6:42340/149‑1 (MQ=255)
            cTTGGGGAAACTCTGCGGGTAGCTGAGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAggc                                                                                                                                            >  6:264066/1‑146 (MQ=255)
            cTTGGGGAAACTCTGCGGGTAGCTGAGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAggc                                                                                                                                            <  5:264066/146‑1 (MQ=255)
                      ctctGCGGGTAGCTGAGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGCCTTGGCCGCCTGCTGGTGGCCAAGccggac                                                                                                                               <  1:327104/149‑4 (MQ=255)
                           cGGGTAGCTGAGGTAGAGCATTGCCAGCGGAATCAGTGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGGGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTg                                                                                                                            <  7:285920/147‑1 (MQ=255)
                            gggTAGCTGAGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGgc                                                                                                                         >  3:92213/1‑149 (MQ=255)
                                 gCTGAGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGt                                                                                                                     <  8:76370/148‑1 (MQ=255)
                                    gAGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCg                                                                                                                 >  8:115106/1‑149 (MQ=255)
                                          gagCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTg                                                                                                            <  3:270032/148‑1 (MQ=255)
                                           agCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTggc                                                                                                          >  7:300528/1‑149 (MQ=255)
                                              aTTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTggcggc                                                                                                       <  8:300528/149‑1 (MQ=255)
                                                     gCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAgcg                                                                                                <  7:115106/149‑1 (MQ=255)
                                                               ggTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTgg                                                                                      >  2:153671/1‑149 (MQ=255)
                                                                      cTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGc                                                                               >  6:298731/1‑149 (MQ=255)
                                                                      cTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGc                                                                               >  6:160623/1‑149 (MQ=255)
                                                                       tttGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCt                                                                              >  5:13812/1‑149 (MQ=255)
                                                                           gCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTcc                                                                           <  6:154735/148‑1 (MQ=255)
                                                                           gCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTcc                                                                           <  1:153671/148‑1 (MQ=255)
                                                                            cccTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAggcgg                                                                                                                                          <  4:224990/84‑1 (MQ=255)
                                                                            cccTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAggcgg                                                                                                                                          >  3:224990/1‑84 (MQ=255)
                                                                             ccTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCtt                                                                         >  5:300538/1‑148 (MQ=255)
                                                                              cTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGa                                                                       <  5:160623/149‑1 (MQ=255)
                                                                                gTTTCGCGGCCTGCTTGAGCGGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGAtt                                                                     >  8:155517/1‑149 (MQ=255)
                                                                                     gcgGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCCCCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCATCGGCTTCATTGATTTGc                                                                  >  2:148750/1‑147 (MQ=255)
                                                                                     aaacccTGCTCAAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGc                                                                  >  7:302974/5‑147 (MQ=255)
                                                                                      cgGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAg                                                               <  4:92213/149‑1 (MQ=255)
                                                                                                 aGCAGGGTTTCGGCTTCCTCCCGGTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGTCTTCCTTGATTTGCGAGGGGTCGCggg                                                     <  1:148750/148‑1 (MQ=255)
                                                                                                   cAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCgg                                                      <  4:179071/145‑1 (MQ=255)
                                                                                                   cAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCgg                                                      >  3:179071/1‑145 (MQ=255)
                                                                                                       gTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCAc                                              >  6:136900/1‑149 (MQ=255)
                                                                                                        tttCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCAcc                                             >  1:308661/1‑149 (MQ=255)
                                                                                                           cGGCTTCCTCACGTTCGGCCTTGGTGCTGTCCTGCTTGGCCACCATCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCtt                                           <  6:300538/148‑1 (MQ=255)
                                                                                                               ttCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGt                                      >  4:66662/1‑149 (MQ=255)
                                                                                                                   tCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGc                                  >  6:53468/1‑149 (MQ=255)
                                                                                                                    cACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTc                                                                                  >  8:179459/1‑100 (MQ=255)
                                                                                                                    cACGTTCGGCCTGCGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTc                                                                                  <  7:179459/100‑1 (MQ=255)
                                                                                                                        ttCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGAt                                        >  1:142841/1‑138 (MQ=255)
                                                                                                                        ttCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGAt                                        <  2:142841/138‑1 (MQ=255)
                                                                                                                            gCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCt                                                                          <  7:31307/100‑1 (MQ=255)
                                                                                                                            gCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCt                                                                          >  8:31307/1‑100 (MQ=255)
                                                                                                                            gCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGAc                         >  8:235988/1‑149 (MQ=255)
                                                                                                                             ccTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGACt                        >  1:87594/1‑149 (MQ=255)
                                                                                                                                    gCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGACTTGCGCt                  >  2:109567/1‑148 (MQ=255)
                                                                                                                                           ggCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGT‑GCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGACTTGCGCTTCGCTGTAg         >  6:95781/1‑149 (MQ=255)
                                                                                                                                             cTTGGCCGCCTGCTGGTGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGt                                                    >  6:341923/1‑105 (MQ=255)
                                                                                                                                             cTTGGCCGCCTGCTGGTGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGt                                                    <  5:341923/105‑1 (MQ=255)
                                                                                                                                             cTTGGCCGCCTGCTGGTGGCCAAGGCCCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATACGTGGCCTCGGCTTCCTTGATTTTCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGACTTGCGCTTCGCTGTAGc        >  2:286263/1‑149 (MQ=255)
                                                                                                                                              ttGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGACTTGCGCTTCGCTGTAGcc       >  2:319314/1‑149 (MQ=255)
                                                                                                                                                ggCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGACTTGCGCTTCGCTGTAGcc       <  5:298731/147‑1 (MQ=255)
                                                                                                                                                   caccaGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGACTTGCGCTTCGCTGTAGCCCAGGt  >  3:26859/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CTGCGCGTTGACCTTGGGGAAACTCTGCGGGTAGCTGAGGTAGAGCATTGCCAGCGGAATCAGGGTATTGCTTTGGCCCTGTTTCGCGGCCTGCTTGAGCAGGGTTTCGGCTTCCTCACGTTCGGCCTGGGTGCTGTCCGGCTTGGCCACCAGCAGGCGGCCAAGGCGCGCTTGGGCGCGTGGCGACGTGGCGGCCGCAGCGCGATAGGTGGCCTCGGCTTCCTTGATTTGCGAGGGGTCGCGGGTGGCCACCTTGATGTCCGCCAGGCCGACTTGCGCTTCGCTGTAGCCCAGGT  >  NC_002947/1469660‑1469955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑