Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,521,248 C→A 7.9% Q169K (CAG→AAG)  murD → UDP‑N‑acetylmuramoylalanine‑‑D‑glutamate ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,521,2480CA 7.9% 192.7 / 6.5 63Q169K (CAG→AAG) murDUDP‑N‑acetylmuramoylalanine‑‑D‑glutamate ligase
Reads supporting (aligned to +/- strand):  ref base C (29/29);  new base A (2/3);  total (31/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.35e-01

ACGCCAAGAGCACCGTCACGACGCTGGTCGGTGAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCGGTGCCCGCCAGGTGGTGG  >  NC_002947/1521102‑1521389
                                                                                                                                                  |                                                                                                                                             
aCGCCAAGAGCACCGTCACGACGCTGGTCGGTGAAATGGCCGCCAAGGCCGGCAAACGCCTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGAACTCCTGCCCGATGACATCGAGCTGTACGTGCTGCAGCTttccagcttccag                                                                                                                                             >  5:175847/1‑149 (MQ=255)
  gCCAAGAGCACCGTCACGACGCTGGTCGGTGAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTtccagcttccagct                                                                                                                                           <  6:278864/149‑1 (MQ=255)
    cAAGAGCACCGTCACGACGCTGTTCGGTGAAATGTCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGTCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGTAGCTTtccagcttccagct                                                                                                                                           <  8:30280/147‑1 (MQ=255)
       tagCACCGTCACGACGCTGGTCGGTGAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTGGAAGCTGGaaa                                                                                                                                      <  5:237745/148‑1 (MQ=255)
       gagCACCGTCACGACGCTGGTCGGTGAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTGGAAGCTGGaaa                                                                                                                                      <  1:297512/149‑1 (MQ=255)
       gagCACCGTCACGACGCTGGTCGGTGAAATGGCCGCCAAGGCCGGCAAACGCGAGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGaaa                                                                                                                                      <  1:282081/149‑1 (MQ=255)
        agCACCGTCACGACGCTGGTCGGTGAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAAc                                                                                                                                     >  8:295145/1‑149 (MQ=255)
            ccGTCACGACGCTGGTCGGTGAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACt                                                                                                                                 <  7:167160/149‑1 (MQ=255)
             cGTCACGACGCGGTTCGGTGAAATGGCCGCCAAGGCAGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGGCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTg                                                                                                                                <  6:175847/149‑1 (MQ=255)
               tCACGACGCTGGTCGGTGAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGAc                                                                                                                              >  1:316482/1‑149 (MQ=255)
               tCACGACGCTGGTCGGTGAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGAc                                                                                                                              >  6:266337/1‑149 (MQ=255)
               tCACGACGCTGGTCGGTGAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGAc                                                                                                                              <  2:316482/149‑1 (MQ=255)
                 acgacgCTGGTCGGTGAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGAc                                                                                                                              >  3:133271/1‑147 (MQ=255)
                  cgacgCTGGTCGGTGAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAg                                                                                                                           <  1:15246/149‑1 (MQ=255)
                             ggTGAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCg                                                                                                                 <  7:295145/148‑1 (MQ=255)
                             ggTGAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGa                                                                                                                <  6:153696/149‑1 (MQ=255)
                              gTGAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCg                                                                                                                 >  5:255421/1‑147 (MQ=255)
                              gTGAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGaa                                                                                                               <  5:148324/149‑1 (MQ=255)
                              gTGAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGaa                                                                                                               <  7:325124/149‑1 (MQ=255)
                                   aTGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGc                                                                                                          >  1:219578/1‑149 (MQ=255)
                                   aTGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGc                                                                                                          <  5:266337/149‑1 (MQ=255)
                                    tGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTGGAAGCTGGaaagctc                                                                                                                                  >  5:74600/1‑120 (MQ=255)
                                    tGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTGGAAGCTGGaaagctc                                                                                                                                  <  6:74600/124‑5 (MQ=255)
                                         gccAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGt                                                                                                    >  6:79300/1‑149 (MQ=255)
                                            aaGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCt                                                                                                 <  6:255421/149‑1 (MQ=255)
                                               gCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGaa                                                                                              >  4:82079/1‑149 (MQ=255)
                                                 cGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGAc                                                                                                                              >  4:297841/1‑115 (MQ=255)
                                                 cGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGAc                                                                                                                              <  3:297841/115‑1 (MQ=255)
                                                         gcgTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGaa                                                                                    <  3:172426/149‑1 (MQ=255)
                                                               ccGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACca                                                                               >  7:164005/1‑148 (MQ=255)
                                                               ccGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACca                                                                               >  6:282788/1‑148 (MQ=255)
                                                                cGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACcaca                                                                             <  5:256330/149‑1 (MQ=255)
                                                                       ggTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGAcc                                                                       >  2:273375/1‑148 (MQ=255)
                                                                       ggTAACCTCGGTACACCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCTAAGTGTCGACCGTTCTGAACATCAGAGAAGACAACATGGAcc                                                                       >  2:269198/1‑148 (MQ=255)
                                                                         tAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCt                                                                    <  4:96938/149‑1 (MQ=255)
                                                                             cTCGGTAGCCCGGCATTCTACCTGCTGGCCGATGACATCGAGATGTAGGTGGTGGAGGTTTCCAGCTTCCAGATGTAAACCACTTTCCAGCTCAAGGCGGAAGTGTCGAGCGTTCTGAACATCAGCGAAGACCACTTGTAcc                                                                       <  8:229873/142‑1 (MQ=255)
                                                                             cTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGAcc                                                                       >  7:229873/1‑142 (MQ=255)
                                                                             cTCGGAACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTCACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAg                                                                >  4:102864/1‑149 (MQ=255)
                                                                                     cccGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTc                                                        >  6:31571/1‑149 (MQ=255)
                                                                                       cGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCg                                                      <  2:325497/149‑1 (MQ=255)
                                                                                        ggCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCgg                                                     <  4:224335/149‑1 (MQ=255)
                                                                                         gCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGc                                                    <  5:79300/149‑1 (MQ=255)
                                                                                          cACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACcac                                                                              >  2:50688/1‑122 (MQ=255)
                                                                                          cACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACcac                                                                              <  1:50688/122‑1 (MQ=255)
                                                                                           aCTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGATCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGCCCGCTATAGCGGCCTTCCGGcc                                                   >  1:320118/1‑148 (MQ=255)
                                                                                             tCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTac                                                >  1:190647/1‑149 (MQ=255)
                                                                                               gACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTac                                                <  3:82079/147‑1 (MQ=255)
                                                                                                aCCTGCTGGCCAATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGCCGACCGTTCTGAACATCAGAGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTaccac                                             >  4:78444/1‑149 (MQ=255)
                                                                                                    gctgGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGcc                                                   >  8:131470/1‑139 (MQ=255)
                                                                                                    gctgGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGcc                                                   <  7:131470/139‑1 (MQ=255)
                                                                                                     ctgGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTg                                          <  5:31571/147‑1 (MQ=255)
                                                                                                         ccGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCaaa                                    >  5:136783/1‑149 (MQ=255)
                                                                                                          cGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCaaa                                    >  5:69610/1‑148 (MQ=255)
                                                                                                                  tCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGAtt                           >  3:147524/1‑149 (MQ=255)
                                                                                                                     aGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTc                        <  2:190647/149‑1 (MQ=255)
                                                                                                                       cTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCcg                      <  6:69610/149‑1 (MQ=255)
                                                                                                                       cTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCcg                      <  3:78444/149‑1 (MQ=255)
                                                                                                                             gTGCTTGAGCTTTCCAGCTTCCAGCTGTAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTTAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCGGTGc                <  1:269198/149‑1 (MQ=255)
                                                                                                                             gTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCGGTGc                <  1:273375/149‑1 (MQ=255)
                                                                                                                             gTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCGGTGc                <  2:219578/149‑1 (MQ=255)
                                                                                                                                 tGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCGGTGCCCGc            >  3:325041/1‑149 (MQ=255)
                                                                                                                                 tGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCGGTGCCCGc            >  6:149076/1‑149 (MQ=255)
                                                                                                                                  ggAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCGGTGCCCGcc           >  7:296261/1‑149 (MQ=255)
                                                                                                                                  ggAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCGGTGCCCGc            >  3:197304/1‑148 (MQ=255)
                                                                                                                                     gCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCGGTGCCCGCCAgg        >  6:122758/1‑149 (MQ=255)
                                                                                                                                       tttCCAGCTGGAAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCGGTGCCCGCCAggtg      >  7:238385/1‑149 (MQ=255)
                                                                                                                                           cagcttccagctGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGc                                        <  6:40958/111‑1 (MQ=255)
                                                                                                                                           cagcttccagctGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGc                                        >  5:40958/1‑111 (MQ=255)
                                                                                                                                           cagcttccagctGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCGGTGCCCGCCAggtggtgg  >  5:41071/1‑149 (MQ=255)
                                                                                                                                           cagcttccagctGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCGGTGCCCGCCAggtggtgg  <  6:140177/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                             
ACGCCAAGAGCACCGTCACGACGCTGGTCGGTGAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCGGTGCCCGCCAGGTGGTGG  >  NC_002947/1521102‑1521389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑