Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,524,047 G→C 7.6% A251P (GCT→CCT)  murG → UDP‑N‑acetylglucosamine‑‑N‑acetylmuramyl‑ (pentapeptide) pyrophosphoryl‑undecaprenol N‑acetylglucosamine transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,524,0470GC 7.6% 146.9 / 2.1 50A251P (GCT→CCT) murGUDP‑N‑acetylglucosamine‑‑N‑acetylmuramyl‑ (pentapeptide) pyrophosphoryl‑undecaprenol N‑acetylglucosamine transferase
Reads supporting (aligned to +/- strand):  ref base G (25/20);  new base C (2/2);  total (27/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

GCCCTGTCCGAGGTGCCCGCGGCGCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGGCCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTCACAACGCCCAATATCTGGCCCGCGA  >  NC_002947/1523906‑1524195
                                                                                                                                             |                                                                                                                                                    
gCCCTGTCCGAGGTGCCCGCGGCGCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATgg                                                                                                                                               >  5:185556/1‑149 (MQ=255)
      tCCGAGGTGCCCGCGGCGCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGCCATAAGCCTggg                                                                                                                                          <  2:179570/148‑1 (MQ=255)
      tCCGAGGTGCCCGCGGCGCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGCCATAAGCCTggg                                                                                                                                          <  5:349895/148‑1 (MQ=255)
            ctggccGCAGCGCCGCGGGCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGGCCGACCt                                                                                                                                   >  4:215810/5‑149 (MQ=255)
                       gcTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGGCCGACCTGGTGGTGTgcc                                                                                                                        <  5:103707/149‑1 (MQ=255)
                             ggcAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGGCCGACCTGGTGGTGTgccgggccg                                                                                                                  <  8:157526/147‑1 (MQ=255)
                                      gtTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGCCTTATGGCTGGTCCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGa                                                                                                         >  5:224160/1‑149 (MQ=255)
                                         ccaccaGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGGCCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTg                                                                                                      >  2:13929/1‑149 (MQ=255)
                                            ccaGGCCGGCAAGCAGCACGCACCGATCACCGCCGCTCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGGCCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCa                                                                                                   <  8:280931/149‑1 (MQ=255)
                                            ccaGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGGCCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGt                                                                                                     >  2:183494/1‑147 (MQ=255)
                                            ccaGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGGCCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCa                                                                                                   <  1:183494/149‑1 (MQ=255)
                                             caGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGGCCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAg                                                                                                  >  3:239777/1‑149 (MQ=255)
                                             caGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGGCCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAg                                                                                                  >  8:306638/1‑149 (MQ=255)
                                                  cGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGGCCGACCTGGTGGTGTGCCGGGCCGGTGCGTTGACTGTCAGCGAAc                                                                                             >  3:62250/1‑149 (MQ=255)
                                                  cGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGGCCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAAc                                                                                             >  7:159399/1‑149 (MQ=255)
                                                  cGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGGCCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAAc                                                                                             >  4:245693/1‑149 (MQ=255)
                                                   ggCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGGCCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACt                                                                                            <  3:215810/149‑1 (MQ=255)
                                                     cAATCAGCACGCACCGATCACCGCCGAGCCTTATGACGAGGCGGGCGTCGCGGCTCAGGTAGATCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGGCCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTgg                                                                                          <  1:260620/149‑1 (MQ=255)
                                                      aaGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGGCCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTgg                                                                                          >  6:182575/1‑148 (MQ=255)
                                                      aaGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGGCCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTgg                                                                                          >  4:195417/1‑148 (MQ=255)
                                                      aaGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGGCCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGgc                                                                                         <  5:182575/149‑1 (MQ=255)
                                                         cagcaCGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGGCCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGgcagc                                                                                      <  8:51814/149‑1 (MQ=255)
                                                           gcacgcacCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGGCCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGgcagcag                                                                                    <  8:159399/149‑1 (MQ=255)
                                                             acgcacCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGGCCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGgcagcag                                                                                    >  3:55320/1‑147 (MQ=255)
                                                              cgcacCGATCACCGCCGAGCGTTCTCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGGCCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGcagcagcag                                                                                 <  3:87257/149‑1 (MQ=255)
                                                                 acCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGGCCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGgcc                                                                              >  8:117322/1‑149 (MQ=255)
                                                                    gATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGGCCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGgcctgc                                                                           <  7:306638/149‑1 (MQ=255)
                                                                        aCCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGGCCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCtt                                                                        <  4:55320/148‑1 (MQ=255)
                                                                               aGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGGCCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTg                                                                <  7:117322/149‑1 (MQ=255)
                                                                               aGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCGTTCATCAAAGACATGGCCCAGGCTTAGGGCTGGGCCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTg                                                                <  1:233453/149‑1 (MQ=255)
                                                                                 cGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGGCCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTg                                                                <  1:285233/147‑1 (MQ=255)
                                                                                 cGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGGCCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTg                                                                >  2:285233/1‑147 (MQ=255)
                                                                                      tCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGGCCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCtt                                                         >  3:295789/1‑149 (MQ=255)
                                                                                          gAGGCGGGCGTCGCGGCTCAGGTAGAGCGTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGGCCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCtt                                                         >  1:194444/1‑145 (MQ=255)
                                                                                          gAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGGCCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCtt                                                         <  2:194444/145‑1 (MQ=255)
                                                                                           aGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGGCCGACCTGGTGGTGTGTCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGccc                                                    >  6:339361/1‑149 (MQ=255)
                                                                                               gggcgTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGGCCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCAcg                                                <  4:239777/149‑1 (MQ=255)
                                                                                                     cgcgGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGGCCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCCGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATcg                                          >  3:60132/1‑149 (MQ=255)
                                                                                                           tCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGGCCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGAt                                     >  4:156803/1‑148 (MQ=255)
                                                                                                              ggTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGGCCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCAcc                                 >  4:318076/1‑149 (MQ=255)
                                                                                                                 agagCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGGCCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGa                              >  2:59346/1‑149 (MQ=255)
                                                                                                                     ccTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGGCCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTca                          >  7:303492/1‑149 (MQ=255)
                                                                                                                     ccTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGGCCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTca                          <  3:318076/149‑1 (MQ=255)
                                                                                                                      cTTTTATCAAAGATTTGGCCCAGTCTTTTTGCTGGGCCGAGCTGGGGGTGTGCGGGGCGGGTGGCTGGACTGTCAGCGAACTGGCAGGAGCTGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGTCGATCACCTGAGTct                          <  4:60132/148‑2 (MQ=255)
                                                                                                                          catcaAAGACATGGCCCAGGCTTATGGCTGGGCCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTCACAAc                      <  3:245693/148‑1 (MQ=255)
                                                                                                                           atcaAAGACATGGCCCAGGCTTATGGCTGGGCCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGt                                                              <  4:207655/107‑1 (MQ=255)
                                                                                                                           atcaAAGACATGGCCCAGGCTTATGGCTGGGCCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGt                                                              >  3:207655/1‑107 (MQ=255)
                                                                                                                            tcaAAGACATGGCCCAGGCTTATGGCTGGGCCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTCACAACGcc                   >  8:263002/1‑149 (MQ=255)
                                                                                                                                        cccAGCCATAAGCCTGGGCCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTCACAACGCCCAATATCTGGcc       >  7:205535/1‑149 (MQ=255)
                                                                                                                                           aGGCTTATGGCTGGGCCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTCACAACGCCCAATATCTGGCCcgc    >  2:337650/1‑149 (MQ=255)
                                                                                                                                             gCTTATGGCTGGGCCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTCACAACGCCCAATATCTGGCCCGCGa  <  4:295789/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                    
GCCCTGTCCGAGGTGCCCGCGGCGCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGGCCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTCACAACGCCCAATATCTGGCCCGCGA  >  NC_002947/1523906‑1524195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑