| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,556,299 | C→T | 6.8% | A68V (GCG→GTG) | purU‑II → | formyltetrahydrofolate hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,556,299 | 0 | C | T | 6.8% | 169.8 / 2.4 | 60 | A68V (GCG→GTG) | purU‑II | formyltetrahydrofolate hydrolase |
| Reads supporting (aligned to +/- strand): ref base C (24/31); new base T (2/2); total (27/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.17e-01 | |||||||||||
AAAGTCAGTAATTTCCTGGCCTTGTACAATGGCTGGATCAACGAAGCCAGCCACCACTCCGATGAGCAGAGCGGTTGGTTCTTCATGCGTCATGAAATCCGCGCTGAATCGCTGCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTGCGCCGATCGCCGAAGAGTTCTCCATGACCTGGCGCATTACCGACTCGGCTCAGAAAAAACGCGTGGTATTGATGGCCAGCCGTGAGTCGCACTGCCTGGCAGACTTGCTGCACCGCTGGCACACCGATGAGCTGGACT > NC_002947/1556151‑1556436 | aaaGTCATTAATTTCCTGGCCTTGTACAAGGGCTGGATCTACGAAGCCAGCCACCACTCCGATGAGCAGAGCGGTTGGTTCTTCATGCGGCATGAAATCCGCGCTGAATCGCTGCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTgc < 6:280631/149‑1 (MQ=255)aaaGTCAGTAATTTCCTGGCCTTGTACAATGGCTGGATCAACGAAGCCAGCCACCACTCCGATGAGCAGAGCGGTTGGTTCTTCATGCGTCATGAAATCCGCGCTGAATCGCTGCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTgc < 5:145164/149‑1 (MQ=255) gTCAGTAATTTCCTGGCCTTGTACAATGGCTGGATCAACGAAGCCAGCCACCACTCCGATGAGCAGAGCGGTTGGTTCTTCATGCGTCATGAAATCCGCGCTGAATCGCTGCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTGCGcc > 6:325554/1‑149 (MQ=255) gTCAGTAATTTCCTGGCCTTGTACAATGGCTGGATCAACGAAGCCAGCCACCACTCCGATGAGCAGAGCGGTTGGTTCTTCATGCGTCATGAAATCCGCGCTGAATCGCTGCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTGCGcc < 2:139274/149‑1 (MQ=255) gTCAGTAATTTCCTGGCCTTGTACAATGGCTGGATCAACGAAGCCAGCCACCACTCCGATGAGCAGAGCGGTTGGTTCTTCATGCGTCATGAAATCCGCGCTGAATCGCTGCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTGCGcc < 5:325554/149‑1 (MQ=255) aGTATTTTCCTGGGCTTTTACATTGGCTGGATCTACGAAGCGAGCCACGACTCCGATGAGCAGAGCGGTTGGTTGTTCATGCGTGATGTATTCCTCGCTGAATCGCTGCCTTTCGGTATCGAAGCCTTCCGTGAGGCGTTTGCGCCGa < 8:155126/148‑1 (MQ=255) aGTAATTTCCTGGCCTTGTACAATGGCTGGATCAACGAAGCCAGCCACCACTCCGATGAGCAGAGCGGTTGGTTCTTCATGCGTCATGAAATCCGCGCTGAATCGCTGCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTGCGCCGa < 6:303293/148‑1 (MQ=255) gTAATTTCCTGGCCTTGTACAATGGCTGGATCAACGAAGCCAGCCACCACTCCGATGAGCAGAGCGGTTGGTTCTTCATGCGTCATGAAATCCGCGCTGAATCGCTGCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTGCGCCGATc < 2:300799/149‑1 (MQ=255) tAATTTCCTGGCCTTGTACAATGGCTGGATCAACGAAGCCAGCCACCACTCCGATGAGCAGAGCGGTTGGTTCTTCATGCGTCATGAAATCCGCGCTGAATCGCTGCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTGTGCCGATCg > 4:78231/1‑149 (MQ=255) ccTGGCCTTGTACAATGGCTGGATCAACGAAGCCAGCCACCACTCCGATGAGCAGAGCGGTTGGTTCTTCATGCGTCATGAAATCCGCGCTGAATCGCTGCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTGCGCCGATCGCCGaa < 6:297147/148‑1 (MQ=255) gCCTTGTACAATGGCTGGATCAACGAAGCCAGCCACCACTCCGATGAGCAGAGCGGTTGGTTCTTCATGCGTCATGAAATCCGCGCTGAATCGCTGCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTGCGCCGATCGCCGAAGAGt < 4:10524/148‑1 (MQ=255) ttGTACAATGGCTGGATCAACGAAGCCAGCCACCACTCCGATGAGCAGAGCGGGTGGTTCTTCATGCGTCATGAAATCCGCGCTGAATCGCTGCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTGTGCCGATCGCCGAAGAGTtctc > 4:214900/1‑149 (MQ=255) gTACAATGGCTGGATCAACGAAGCCAGCCACCACTCCGATGAGCAGAGCGGTTGGTTCTTCATGCGTCATGAAATCCGCGCTGAATCGCTGCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTGCGCCGATCGCCGAAGAGTTCTcc < 7:153339/148‑1 (MQ=255) cAATGGCTGGATCAACGAAGCCAGCCACCACTCCGATGAGCAGAGCGGTTGGTTCTTCATGCGTCATGAAATCCGCGCTGAATCGCTGCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTGCGCCGATCGCCGAAGAGTTCTCCATGa < 8:286336/149‑1 (MQ=255) aaTGGCTGGATCAACGAAGCCAGCCACCACTCCGATGAGCAGAGCGGTTGGTTCTTCATGCGTCATGAAATCCGCGCTGAATCGCTGCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTGCGCCGATCGCCGAAGAGTTCTCCATGAc > 1:331437/1‑149 (MQ=255) tGGCTGGATCAACGAAGCCAGCCACCACTCCGATGAGCAGAGCGGTTGGTTCTTCATGCGTCATGAAATCCGCGCTGAATCGCTGCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTGCGCCGATCGCCGAAGAGTTCTCCATg > 7:214350/1‑145 (MQ=255) tGGCTGGATCAACGAAGCCAGCCACCACTCCGATGAGCAGAGCGGTTGGTTCTTCATGCGTCATGAAATCCGCGCTGAATCGCTGCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTGCGCCGATCGCCGAAGAGTTCTCCATg < 8:214350/145‑1 (MQ=255) gCTGGATCAACGAAGCCAGCCACCACTCCGATGAGCAGAGCGGTTGGTTCTTCATGCGTCATGAAATCCGCGCTGAATCGCTGCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTGCGCCGATCGCCGAAGAGTTCTCCATGACCTgg > 5:107007/1‑149 (MQ=255) cTGGATCAACGAAGCCAGCCACCACTCCGATGAGCAGAGCGGGTGGTTCTTCATGCGTCATGAAATCCGCGCTGAATCGCTGCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTGCGCCGATCGCCGAAGAGTTCTCCATGACCTGgc < 7:306359/149‑1 (MQ=255) gATCAACGAAGCCAGCCACCACTCCGATGAGCAGAGCGGTTGGTTCTTCATGCGTCATGAAATCCGCGCTGAATCGCTGCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTGCGCCGATCGCCGAAGAGTTCTCCATGACCTGGCGCa > 2:40994/1‑149 (MQ=255) aaCGAAGCCAGCCACCACTCCGATGAGCAGAGCGGTTGGTTCTTCATGCGTCATGAAATCCGCGCTGAATCGCTGCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTGCGCCGATCGCCGAAGAGTTCTCCATGACCTGGCGCATTa < 6:107007/148‑1 (MQ=255) aCGAAGCCAGCCACCACTCCGATGAGCAGAGCGGTTGGTTCTTCATGCGTCATGAAATCCGCGCTGAATCGCTGCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTGCGCCGATCGCCGAAGAGTTCTCCATGACCTGGCGCATTAcc < 1:40994/149‑1 (MQ=255) gAAGCCAGCCACCACTCCGATGAGCAGAGCGGTTGGTTCTTCATGCGTCATGAAATCCGCGCTGAATCGCTGCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTGTGCCGATCGCCGAAGAGTTCTCCATGACCTGGCGCATTACCGa < 3:214900/149‑1 (MQ=255) gccaCCACTCCGATGAGCAGAGCGGTTGGTTCTTCATGCGTCATGAAATCCGCGCTGAATCGCTGCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTGCGCCGATCGCCGAAGAGTTCTCCATGACCTGGCGCATTACCGACTCGGCt < 2:331437/149‑1 (MQ=255) gccaCCACTCCGATGAGCAGAGCGGTTGGTTCTTCATGCGTCATGAAATCCGCGCTGAATCGATGCCGTTCGGTATAGAAGCCTTCCGTGAGGCGTTTGCGACGATCGCCGAAGAGTTCTCCATGACCTGGCGCATTACCTACTAGGc > 2:257083/1‑148 (MQ=255) aaacacTCAGATGAGCAGAGCGGTTGGTTCTTCATGCGTCATGAAATCCGCGCTGAATCCCTTCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTGCCCCGATCGCCGAAGAGTTCTCCATGACCTGGCGCATTACCGACTCGGCTCa < 1:257083/146‑1 (MQ=255) acTCCGATGAGCAGAGCGGTTGGTTCTTCATGCGTCATGAAATCCGCGCTGAATCGCTGCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTGCGCCGATCGCCGAAGAGTTCTCCATGACCTGGCGCATTACCGACTCGGCTCAGaa > 2:224291/1‑148 (MQ=255) gATGAGCAGAGCGGTTGGTTCTTCATGCGTCATGAAATCCGCGCTGAATCGCTGCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTGCGCCGATCGCCGAAGAGTTCTCCATGACCTGGCGCATTACCGACTCGGCTCAGAAAAAAcg > 6:268864/1‑149 (MQ=255) aTGAGCAGAGCGGTTGGTTCTTCATGCGTCATGAAATCCGCGCTGAATCGCTGCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTGCGCCGATCGCCGAAGAGTTATCCATGACCTGGCGCATTACCGACTCGGCTCAGAAAAAAcg > 7:67651/1‑148 (MQ=255) gagCGGTTGGTTCTTCATGCGTCATGAAATCCGCGCTGAATCGCTGCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTGCGCCGATCGCCGAAGAGTTCTCCATGACCTGGCGCATTACCGACTCGGCTCAGAAAAAACGCGTGGTAt < 5:268864/149‑1 (MQ=255) gagCGGTTGGTTCTTCATGCGTCATGAAATCCGCGCTGAATCGCTGCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTGCGCCGATCGCCGAAGAGTTCTCCATGACCTGGCGCATTACCGACTCGGCTCAGAAAAAACGCGTGGTAt < 2:85734/149‑1 (MQ=255) aTGCGTCATGAAATCCGCGCTGAATCGCTGCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTGCGCCGATCGCCGAAGAGTTCTCCATGACCTGGCGCATTACCGACTCGGCTCAGAAAAAAcgc < 7:82265/126‑1 (MQ=255) aTGCGTCATGAAATCCGCGCTGAATCGCTGCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTGCGCCGATCGCCGAAGAGTTCTCCATGACCTGGCGCATTACCGACTCGGCTCAGAAAAAAcgc > 8:82265/1‑126 (MQ=255) gCGTCATGAAATCCGCGCTGAATCGCTGCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTGCGCCGATCGCCGaa > 7:268234/1‑76 (MQ=255) gCGTCATGAAATCCGCGCTGAATCGCTGCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTGCGCCGATCGCCGaa < 8:268234/76‑1 (MQ=255) gTCATGAAATCCGCGCTGAATCGCTGCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTGCGCCGATCGCCGAAGAGTTCTCCATGACCTGGCGCATTACCGACTCGGCTCAGAAAAAACGCGt < 4:108795/124‑1 (MQ=255) gTCATGAAATCCGCGCTGAATCGCTGCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTGCGCCGATCGCCGAAGAGTTCTCCATGACCTGGCGCATTACCGACTCGGCTCAGAAAAAACGCGt > 3:108795/1‑124 (MQ=255) gTCATGAAATCCGCGCTGAATCGCTGCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTGCGCCGATCGCCGAAGAGTTCTCCATGACCTGGCGCATTACCGACTCGGCTCAGAAAAAACGCGTGGTATTGATGGCCAGCCGTGAGTCg > 8:194320/1‑149 (MQ=255) aTGAAATCCGCGCTGAATCGCTGCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTGCGCCGATCGCCGAAGAGTTCTCCATGACCTGGCGCATTACCGACTCGGCTCAGAAAAAACGCGTGGTATTGAt < 4:121092/130‑1 (MQ=255) aTGAAATCCGCGCTGAATCGCTGCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTGCGCCGATCGCCGAAGAGTTCTCCATGACCTGGCGCATTACCGACTCGGCTCAGAAAAAACGCGTGGTATTGAt > 3:121092/1‑130 (MQ=255) aTGAAATCCGCGCTGAATCGCTGCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTGCGCCGATCGCCGAAGAGTTCTCCATGACCTGGCGCATTACCGACTCGGCTCAGAAAAAACGCGTGGTATTGATGGCCAGCCGTGAGTCGCa < 1:224291/148‑1 (MQ=255) agAAATCCGCGCTGAATCGCTGCCGTTAGGTATCGAAGCCTTACGTGAGGAGTTAGAGCCGATCGCCCAAGAATACACCATGACCTGCCGCATTACCGACTCGGCTCAGAAAAAACGCGTGGTATTGATGGCCAGCAGTGAGTCGCaat > 2:263648/2‑147 (MQ=255) gAAATCCGCGCTGAATCGCTGCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTGCGCCGATCGCCGAAGAGTTCTCCATGACCTGGCGCATTAcc > 1:100332/1‑96 (MQ=255) gAAATCCGCGCTGAATCGCTGCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTGCGCCGATCGCCGAAGAGTTCTCCATGACCTGGCGCATTAcc < 2:100332/96‑1 (MQ=255) gAAATCCGCGCTGAATCGCTGCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTGCGCCGATCGCCGAAGAGTTCTCCATGACCTGGCGCATTACCGACTCGGCTCAGAAAAAACGCGTGGTATTGATGGCCAGCCGTGAGTCGCACTg > 7:149904/1‑149 (MQ=255) tCCGCGCTGAATCGCTGCCGTTCGGTATCGAAGCCTTACGTGAGGCGTTTGCGCCGATCGCCGAAGAGTTCTCCATGACCTGGCGCATTACCGACTCGGCTCAGAAAAACCGCGTGGTCTTGATGGCCAGCCGTGAGTCGCACTGCCTg > 4:234041/1‑149 (MQ=255) aTCGCTGCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTGCGCCGATCGCCGAAGAGTTCTCCATGACCTGGCGCATTACCGACTCGGCTCAGAAAAAACGCGTGGTATTGATGGCCAGCCGTGAGTCGCACTGCCTGGCAGACTtgc < 5:119357/149‑1 (MQ=255) aTCGCTGCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTGCGCCGATCGCCGAAGAGTTCTCCATGACCTGGCGCATTACCGACTCGGCTCAGAAAAAACGCGTGGTATTGATGGCCAGCCGTGAGTCGCACTGCCTGGCAGACTtgc < 8:279041/149‑1 (MQ=255) tCGCTGCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTGCGCCGATCGCCGAAGAGTTCTCCATGACCTGGCGCATTACCGACTCGGCTCAGAAAAAACGCGTGGTATTGATGGCCAGCCGTGAGTCGCACTGCCTGGCAGACTtgct > 3:135919/1‑149 (MQ=255) cTGCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTGCGCCGATCGCCGAAGAGTTCTCCATGACCTGgcg < 6:60107/71‑1 (MQ=255) cTGCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTGCGCCGATCGCCGAAGAGTTCTCCATGACCTGgcg > 5:60107/1‑71 (MQ=255) cTGCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTGCGCCGATCGCCGAAGAGTTCTCCATGACCTGGCGCATTACCGACTCGGCTCAGAAAAAACGCGTGGTATTGATGGCCAGCCGTGAGTCGCACTGCCTGGCAGACTTGCTGCa < 2:19887/149‑1 (MQ=255) gCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTGCGCCGATCGCCGAAGAGTTCTCCATGACCTGGCGCATTACCGACTCGGCTCAGAAAAAACGCGTGGTATTGATGGCCAGCCGTGAGTCGCACt < 8:49269/128‑1 (MQ=255) gCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTGCGCCGATCGCCGAAGAGTTCTCCATGACCTGGCGCATTACCGACTCGGCTCAGAAAAAACGCGTGGTATTGATGGCCAGCCGTGAGTCGCACt > 7:49269/1‑128 (MQ=255) gCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTGCGCCGATCGCCGAAGAGTTCTCCATGACCTGGCGCATTACCGACTCGGCTCAGAAAAAACGCGTGGTATTGATGGCCAGCCGTGAGTCGCACTGCCTGGCa < 3:84536/136‑1 (MQ=255) gCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTGCGCCGATCGCCGAAGAGTTCTCCATGACCTGGCGCATTACCGACTCGGCTCAGAAAAAACGCGTGGTATTGATGGCCAGCCGTGAGTCGCACTGCCTGGCa > 4:84536/1‑136 (MQ=255) gTATCGAAGCCTTCCGTGAGGCGTTTGCGCCGATCGCCGAAGAGTTCTCCATGACCTGGCGCATTACCGACTCGGCTCAGAAAAAACGCGTGGTATTGATGGCCAGCCGTGAGTCGCACTGCCTGGCAGACTTGCTGCACCGCTGGcac > 8:311624/1‑149 (MQ=255) aTCGAAGCCTTCCGTGAGGCGTTTGCGCCGATCGCCGAAGAGTTCTCCATGACCTGGCGCATTACCGACTCGGCTCAGAAAAAACGCGTGGTATTGATGGCCAGCCGTGAGTCGCACTGCCTGGCAGACTTGCTGCACCGCTGGCacac > 1:316000/1‑149 (MQ=255) gAAGCCTTACGTGAGGCGTTTGCGCCGATCGCCGAAGAGTTCTCCATGa > 4:53990/1‑49 (MQ=255) aGCCTTCCGTGAGGCGTTTGTGCCGATCGCCGAAGAGTTCTCCATGACCTGGCGCATTACCGACTCGGCTCAGAAAAAACGCGTGGTATTGATGGCCAGCCGTGAGTCGCACTGCCTGGCAGACTTGCTGCACCGCTGGCACACCGATg < 3:78231/149‑1 (MQ=255) gCCTTCCGTGAGGCGTTTGCGCCGATCGCCGAAGAGTTCTCCATGACCTGGCGCATTACCGACTCGGCTCAGAAAAAACGCGTGGTATTGATGGCCAGCCGTGAGTCGCACTGCCTGGCAGACTTGCTGCACCGCTGGCACACCGATGa < 5:155921/149‑1 (MQ=255) tGAGGCGTTTGCGCCGATCGCCGAAGAGTTCTCCATGACCTGGCGCATTACCGACTCGGCTCAGAAAAAACGCGTGGTATTGATGGCCAGCCGTGAGTCGCACTGCCTGGCAGACTTGCTGCACCGCTGGCACACCGATGAGCTGGACt < 7:194320/149‑1 (MQ=255) | AAAGTCAGTAATTTCCTGGCCTTGTACAATGGCTGGATCAACGAAGCCAGCCACCACTCCGATGAGCAGAGCGGTTGGTTCTTCATGCGTCATGAAATCCGCGCTGAATCGCTGCCGTTCGGTATCGAAGCCTTCCGTGAGGCGTTTGCGCCGATCGCCGAAGAGTTCTCCATGACCTGGCGCATTACCGACTCGGCTCAGAAAAAACGCGTGGTATTGATGGCCAGCCGTGAGTCGCACTGCCTGGCAGACTTGCTGCACCGCTGGCACACCGATGAGCTGGACT > NC_002947/1556151‑1556436 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |