Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,562,479	C→T	5.9%	A74V (GCG→GTG)	PP_1372 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,562,479	0	C	T	5.9%	196.8 / 2.1	67	A74V (GCG→GTG)	PP_1372	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (32/31);  new base T (2/2);  total (34/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01

GCCAACCGCCACGGTCTGGTGGCTGGCGCAACCGGTACTGGCAAGACCGTCACCCTGCAGCGCCTGGCGGAGGTGTTCAGCGACGCAGGGGTGGCGGTGTTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCGTGGCTGGGGCATACGCCCAGGGCTTACCCGGTGAGCTTGTGGGATATCGCCGGGCAGTCTGGCCACCCGCTGCGCACCACCCTCAGTGAAATGGGGCC  >  NC_002947/1562331‑1562617                                                                                                                                                      |                                                                                                                                           gCCAACCGCCACGGTCTGGTGGCTGGCGCAACCGGTACTGGCAAGACCGTCACCCTGCAGCGCCTGGCGGAGGTGTTCAGCGACGCAGGGGTGGCGGTGTTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTgc                                                                                                                                            <  6:40220/149‑1 (MQ=255) gCCAACCGCCACGGTCTGGTGGCTGGCGCAACCGGTACTGGCAAGACCGTCACCCTGCAGCGCCTGGCGGAGGTGTTCAGCGACGCAGGGGTGGCGGTGTTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTgc                                                                                                                                            >  3:275677/1‑149 (MQ=255)   cAACCGCCACGGTCTGGTGGCTGGCGCAACCGGTACTGGCAAGACCGTCACCCTGCAGCGCCTGGCGGAGGTGTTCAGCGACGCAGGGGTGGCGGTGTTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTgcgc                                                                                                                                          >  8:262507/1‑149 (MQ=255)   cAACCGCCACGGTCTGGTGGCTGGCGCAACCGGTACTGGCAAGACCGTCACCCTGCAGCGCCTGGCGGAGGTGTTCAGCGACGCAGGGGTGGCGGTGTTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTgcgc                                                                                                                                          >  5:116669/1‑149 (MQ=255)    aaCCGCCACGGTCTGGTGGCTGGCGCAACCGGTACTGGCAAGACCGTCACCCTGCAGCGCCTGGCGGAGGTGTTCAGCGACGCAGGGGTGGCGGTGTTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTgcgc                                                                                                                                          >  1:132221/1‑148 (MQ=255)                          gcgcAACCGGTACTGGCAAGACCGTCACCCTGCAGCGCCTGGCGGAGGTGTTCAGCGACGCAGGGGTGGCGGTGTTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTGCGCCACAGGGCAAGGTGGCTGAgc                                                                                                                     <  6:139446/147‑1 (MQ=255)                            gcAACCGGTACTGGCAAGACCGTCACCCTGCAGCGCCTGGCGGAGGTGTTCAGCGACGCAGGGGTGGCGGTGTTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTGCGCCACAGGGCAAGGTGGCTGAGCGCAt                                                                                                                 >  6:274708/1‑149 (MQ=255)                             cAACCGGTACTGGCAAGACCGTCACCCTGCAGCGCCTGGCGGAGGTGTTCAGCGACGCAGGGGTGGCGGTGTTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTGCGCCACAGGGCAAGGTGGCTGAGCGCATc                                                                                                                >  7:263095/1‑149 (MQ=255)                                   gTAATGGCAAGAAAGTCAACCTGCACCTCCTGGCGGAGGTGTACAGCGACGCAGGGGTTGCGGTCTTCGACGCGGATGTC‑AAAGGCGACCTATATGGACTCGGTGCTCCAGGATCGCAACAGGGAAAGAAGTCTGAGAGCATCgacat                                                                                                           >  8:307306/1‑144 (MQ=255)                                      cTGGCAAGACCGTCACCCTGCAGCGCCTGGCGGAGGTGTTCAGCGACGCAGGGGTGGCGGTGTTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCAt                                                                                                        <  4:275677/148‑1 (MQ=255)                                       tGGCAAGACCGTCACCCTGCAGCGCCTGGCGGAGGTGTTCAGCGACGCAGGGGTGGCGGTGTTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGc                                                                                                      >  1:229587/1‑149 (MQ=255)                                         gCAAGACCGTCACCCTGCAGCGCCTGGCGGAGGTGTTCAGCGACGCAGGGGTGGCGGTGTTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCg                                                                                                    >  6:14642/1‑149 (MQ=255)                                         gCAAGACCGTCACCCTGCAGCGCCTGGCGGAGGTGTTCAGCGACGCAGGGGTGGCGGTGTTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCg                                                                                                    >  4:239764/1‑149 (MQ=255)                                          cAAGACCGTCACCCTGCAGCGCCTGGCGGAGGTGTTCAGCGACGCAGGGGTGGCGGTGTTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGc                                                                                                      >  8:187738/1‑146 (MQ=255)                                          cAAGACCGTCACCCTGCAGCGCCTGGCGGAGGTGTTCAGCGACGCAGGGGTGGCGGTGTTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGc                                                                                                      <  7:187738/146‑1 (MQ=255)                                               ccGTCACCCTGCAGCGCCTGGCGGAGGTGTTCAGCGACGCAGGGGTGGCGGTGTTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTGTGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCGTGGCTg                                                                                              >  8:77280/1‑149 (MQ=255)                                                cGTCACCCTGCAGCGCCTGGCGGAGGTGTTCAGCGACGCAGGGGTGGCGGTGTTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTGCGCCACAggg                                                                                                                                   <  3:125208/111‑1 (MQ=255)                                                cGTCACCCTGCAGCGCCTGGCGGAGGTGTTCAGCGACGCAGGGGTGGCGGTGTTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTGCGCCACAggg                                                                                                                                   >  4:125208/1‑111 (MQ=255)                                                  tCACCCTGCAGCGCCTGGCGGAGGTGTTCAGCGACGCAGGGGTGGCGGTGTTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCGTGGCTgggg                                                                                           >  2:77814/1‑149 (MQ=255)                                                     cccTGCAGCGCCTGGCGGAGGTGTTCAGCGACGCAGGGGTGGCGGTGTTCGCCGCGGATGTCTAAAGGCGACCTTTGTGGCCTGGGTGATGCCGGTGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCGTGGCTGGGGCa                                                                                         >  1:275497/1‑149 (MQ=255)                                                      catGCAGCGCCTGGCGGAGGTGTTCAGCGACGCAGGGGTGGCGGTGTTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCGTGGCTGGGGCATa                                                                                       >  7:295500/3‑149 (MQ=255)                                                            gcgcCTGGCGGAGGTGTTCAGCGACGCAGGGGTGGCGGTGTTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCGTGGCTGGGGCATACGcc                                                                                   >  6:75184/1‑147 (MQ=255)                                                             cgcCTGGCGGAGGTGTTCAGCGACGCAGGGGTGGCGGTGTTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCGTgg                                                                                                 <  6:9726/132‑1 (MQ=255)                                                             cgcCTGGCGGAGGTGTTCAGCGACGCAGGGGTGGCGGTGTTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCGTgg                                                                                                 >  5:9726/1‑132 (MQ=255)                                                             cgcCTGGCGGAGGTGTTCAGCGACGCAGGGGTGGCGGTGTTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCGTGGCTGGGGCATACGCCCAg                                                                                >  2:218221/1‑149 (MQ=255)                                                              gcCTGGCGGAGGTGTTCAGCGACGCAGGGGTGGCGGTGTTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCGTGTCTGGGGCATACGCCCAgg                                                                               <  2:229587/149‑1 (MQ=255)                                                              gcCTGGCGGAGGTGTTCAGCGACGCAGGGGTGGCGGTGTTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCGTGGCTGGGGCATACGCCCAgg                                                                               <  7:262507/149‑1 (MQ=255)                                                              gcCTGGCGGAGGTGTTCAGCGACGCAGGGGTGGCGGTGTTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCGTGGCTGGGGCATACGCCCAgg                                                                               <  1:77814/149‑1 (MQ=255)                                                              gcCTGGAGGAGGTGTTCAGCGACGCAGGGGTGGCGGTGTTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGAGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCGTGGCTGGGGCATACGCCCAgg                                                                               <  7:307306/149‑1 (MQ=255)                                                              accTGGCGGAGGTGTTCAGCGACGCAGGGGTGGCGGTGTTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCGTGGCTGGGGCATACGCCCAgg                                                                               >  8:102062/2‑149 (MQ=255)                                                               ccTGGCGGAGGTGTTCAGCGACGCAGGGGTGGCGGTGTTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCGTGGCTGGGGCATACGCCCAggg                                                                              <  2:132221/149‑1 (MQ=255)                                                                cTGGCGGAGGTGTTCAGCGACGCAGGGGTGGCGGTGTTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCGTGGCTGGGGCATACGCCCAGGGc                                                                             >  6:334074/1‑149 (MQ=255)                                                                   gCGGAGGTGTTCAGCGACGCAGGGGTGGCGGTGTTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCGTGGCTGGGGCATACGCCCAGGGCTTa                                                                          <  5:274708/149‑1 (MQ=255)                                                                    cGGAGGTGTTCAGCGACGCAGGGGTGGCGGTGTTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCGTGGCTGGGGCATACGCCCAGGGCTTAc                                                                         <  6:116669/149‑1 (MQ=255)                                                                     ggAGGTGTTCAGCGACGCAGGGGTGGCGGTGTTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCGTGGCTGGGGCATACGCCCAGGGCTTAcc                                                                        >  7:275021/1‑149 (MQ=255)                                                                      gAGGTGTTCAGCGACGCAGGGGTGGCGGTGTTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCGTGGCTGGGGCATACGCCCAGGGCTTAccc                                                                       <  2:180412/149‑1 (MQ=255)                                                                        ggTGTTCAGCGACGCAGGGGTGGCGGTGTTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCGTGGCTGGGGCATACGCCCAGGGCTTACCCgg                                                                     <  7:102062/149‑1 (MQ=255)                                                                         gtgtTCAGCGACGCTGGGGTGGCGGTGTTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCGGGGTGCTGCCGGTGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCGTGGCTGGGGCATACGCCCAGGGCTTACCCGGt                                                                    <  5:14642/149‑1 (MQ=255)                                                                         gtgtTCAGCGACGCAGGGGTGGCGGTGTTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCGTGGCTGGGGCATACGCCCAGGGCTTACCCGGt                                                                    <  5:75184/149‑1 (MQ=255)                                                                          tgtTCAGCGACGCAGGGGTGGCGGTGTTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCGTGGCTGGGGCATACGCCCAGGGCTTACCCGGTg                                                                   <  1:218221/149‑1 (MQ=255)                                                                           gtTCAGCGACGCAGGGGTGGCGGTGTTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCGTGGCTGGGGCATACGCCCAGGGCTTACCCGGt                                                                    >  2:254282/1‑147 (MQ=255)                                                                            ttCAGCGACGCAGGGGTGGCGGTGTTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCGTGGCTGGGGCATACGCCCAGGGCTTACCCGGTGa                                                                  <  2:275497/148‑1 (MQ=255)                                                                              cAGCGACGCAGGGGTGGCGGTGTTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTGTGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCGTGGCTGGGGCATACGCCCAGGGCTTACCCGGTGAGCt                                                               >  4:191593/1‑149 (MQ=255)                                                                                 cGACGCAGGGGTGGCGGTGTTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTGCGCcac                                                                                                                                       >  4:68568/1‑74 (MQ=255)                                                                                 cGACGCAGGGGTGGCGGTGTTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTGCGCcac                                                                                                                                       <  3:68568/74‑1 (MQ=255)                                                                                 cGACGCAGGGGTGGCGGTGTTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCGTGGCTGGGGCATACGCCCAGGGCTTACCCGGTGAGGTtgt                                                            >  8:298703/1‑149 (MQ=255)                                                                                 cGACGCAGGGGTGGCGGTGTTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCGTGGCTGGGGCATACGCCCAGGGCTTACCCGGTGAGCTtgt                                                            <  3:239764/149‑1 (MQ=255)                                                                                   aCGCAGGGGTGGCGGTGTTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCGTGGCTGGGGCATACGCCCAGGGCTTACCCGGTGAGCTTGTgg                                                          >  5:106454/1‑149 (MQ=255)                                                                                    cGCAGGGGTGGCGGTGTTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCGTGGCTGGGGCATACGCCCAGGGCTTACCCGGTGAGCTTGTggg                                                         <  8:164609/149‑1 (MQ=255)                                                                                         gggTGGCGGTGTTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCGTGGCTGGGGCATACGCCCAGGGCTTACCCGGTGAGCTTGTGGGATATc                                                    >  3:165328/1‑149 (MQ=255)                                                                                             ggCGGTGTTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCGTGGCTGGGGCATACGCCCAGGGCTTACCCGGTGAGCTTGTGGGATATCGCCg                                                <  8:263095/149‑1 (MQ=255)                                                                                               cGGTGTTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCGTGGCTGGGGCATACGCCCAGGGCTTACCCGGTGAGCTTGTGGGATATCGCCggg                                              >  8:182934/1‑149 (MQ=255)                                                                                               cGGTGTTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCGTGGCTGGGGCATACGCCCAGGGCTTACCCGGTGAGCTTGTGGGATATCGCCggg                                              <  4:252237/149‑1 (MQ=255)                                                                                                 gtgtTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTGCGCCAGAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCGTGGGTGGGGCATACGCCCAGGGCTTACCCGGTGAGCTTGTGGGATATCGCCGGGCa                                            <  8:295500/149‑1 (MQ=255)                                                                                                 gtgtTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCGTGGCTGGGGCATACGCCCAGGGCTTACCCGGTGAGCTTGTGGGATATCGCCGGGCa                                            <  2:90963/149‑1 (MQ=255)                                                                                                        ccgcGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCGTGGCTGGGGCATACGCCCAGGGCTTACCCGGTGAGCTTGTgg                                                          <  3:232699/128‑1 (MQ=255)                                                                                                        ccgcGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCGTGGCTGGGGCATACGCCCAGGGCTTACCCGGTGAGCTTGTgg                                                          >  4:232699/1‑128 (MQ=255)                                                                                                                 tC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCGTGGCTGGGGCATACGCCCAGGGCTTACCCGGTGAGCTTGTGGGATATCGCCGGGCAGTCTGGCCACCCGCTg                            <  7:182934/149‑1 (MQ=255)                                                                                                                  c‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCGTGGCTGGGGCATACGCCCAGGGCTTACCCGGTGAGCTTGTGGGATATCGCCGGGCAGTCTGGCCACCCGCTgc                           >  4:331842/1‑149 (MQ=255)                                                                                                                     aaGGCGACCTTTGTGGCCTGGGTGCTGCCGGTGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCGTGGCTGGGGCATACGCCCAGGGCTTACCCGGTGAGCTTGTGGGATATCGCCGGGCAGTCTGGCCACCCGCTgcgc                         >  8:108315/1‑149 (MQ=255)                                                                                                                             cTTTGTGGCTTGGGTGCTGCCGGTGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCGTGGCTGGGGCATACGCCCAGGGCTTACCCGGTGAGCTTGTGGGATATCGCCGGGCAGTCTGGCCACCCGCTGCGCACCACCCt                 >  4:72287/1‑149 (MQ=255)                                                                                                                             cTTTGTGGCCTGGGTGCTGCCGGTGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCGTGGCTGGGGCATACGCCCAGGGCTTACCCGGTGAGCTTGTGGGATATCGCCGGGCAGTCTGGCCACCCGCTGCGCaccacc                   >  6:331719/1‑147 (MQ=255)                                                                                                                             cTTTGTGGCCTGGGTGCTGCCGGTGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCGTGGCTGGGGCATACGCCCAGGGCTTACCCGGTGAGCTTGTGGGATATCGCCGGGCAGTCTGGCCACCCGCTGCGCaccacc                   <  5:331719/147‑1 (MQ=255)                                                                                                                                 gtgGCCTGGGTGCTGCCGGTGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCGTGGCTGGGGCATACGCCCAGGGCTTACCCGGTGAGCGTGTGGGATATCGCCGGGCAGTCTGGCCACCCGCTGCGCACCACCCTCAg              <  2:283916/148‑1 (MQ=255)                                                                                                                                  tgGCCTGGGTGCTGCCGGTGTGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCGTGGCTGGGGCATACGCCCAGGGCTTACCCGGTGAGCTTGTGGGATATCGCCGGGCAGTCTGGCCACCCGCTGCGCACCACCCTCAGt             <  3:191593/148‑1 (MQ=255)                                                                                                                                      cTGGGTGCTGCCGGTGCGCCACAGGACAAGGTGGCTGAACGCATCGCCGGCATGCCGTGGCTGGGGCATACGCCCAGGGCTTACCCGGTGAGCTTGTGGTATATCGCCGGGCAGTCTGGCCACCCGATGCGCACCACCCACAGTGAAAt        >  8:51107/1‑149 (MQ=255)                                                                                                                                         ggTGCTGCCGGTGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCGTGGCTGGGGCATACGCCCAGGGCTTACCCGGTGAGCTTGTGGGATATCGCCGGGCAGTCTGGCCACCCGCTGCGCACCACCCTCAGTGAAATggg     >  4:228922/1‑149 (MQ=255)                                                                                                                                          gTGCTGCCGGTGTGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCGTGGCTGGGGCATACGCCCAGGGCTTACCCGGTGAGCTTGTGGGATATCGCCGGGCAGTCTGGCCACCCGCTGCGCACCACCCTCAGTGAAATgggg    <  7:77280/149‑1 (MQ=255)                                                                                                                                           tgctgcCGGTGCGCCTCAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCGGTGGCTGGGGCATACGGCCAGGGCTTACCCGGTGAGCTTGTGGGATATCGCCGGGCAGTCTGGCCACCCGCTGCGCACCACCCTCAGTGAAATGGGGc   <  4:165328/149‑1 (MQ=255)                                                                                                                                            gctgcCGGTGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCGTGGCTGGGGCATACGCCCAGGGCTTACCCGGTGAGCTTGTGGGATATCGCCGGGCAGTCTGGCCACCCGCTGCGCACCACCCTCAGTGAAATGGGGcc  >  1:147041/1‑149 (MQ=255)                                                                                                                                                   gTGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCGTGGCTGGGGCATACGCCCAGGGCTTACCCGGTGAGCTTGTGTGATATCGCCGGGGAGTCTGGCCACCCGCTGCGCACCACCCTCAGt             <  6:219793/131‑1 (MQ=255)                                                                                                                                                   gTGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCGTGGCTGGGGCATACGCCCAGGGCTTACCCGGTGAGCTTGTGGGATATCGCCGGGCAGTCTGGCCACCCGCTGCGCACCACCCTCAGt             >  5:219793/1‑131 (MQ=255)                                                                                                                                                      |                                                                                                                                           GCCAACCGCCACGGTCTGGTGGCTGGCGCAACCGGTACTGGCAAGACCGTCACCCTGCAGCGCCTGGCGGAGGTGTTCAGCGACGCAGGGGTGGCGGTGTTCGCCGCGGATGTC‑AAAGGCGACCTTTGTGGCCTGGGTGCTGCCGGTGCGCCACAGGGCAAGGTGGCTGAGCGCATCGCCGGCATGCCGTGGCTGGGGCATACGCCCAGGGCTTACCCGGTGAGCTTGTGGGATATCGCCGGGCAGTCTGGCCACCCGCTGCGCACCACCCTCAGTGAAATGGGGCC  >  NC_002947/1562331‑1562617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑