| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,570,438 | A→C | 13.2% | intergenic (+46/‑142) | pcaF‑I → / → pcaT | beta‑ketoadipyl‑CoA thiolase/alpha‑ketoglutarate permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,570,438 | 0 | A | C | 13.2% | 117.2 / 3.3 | 56 | intergenic (+46/‑142) | pcaF‑I/pcaT | beta‑ketoadipyl‑CoA thiolase/alpha‑ketoglutarate permease |
| Reads supporting (aligned to +/- strand): ref base A (24/24); new base C (3/5); total (27/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.07e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCG‑CGGGGAAGCCCGCTCCAACAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCAGGAGTAAACCCGTCATGACC > NC_002947/1570293‑1570585 | tgCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCG‑CGGGGAAGCCCGCTCCAACAGG‑GATCCACg > 8:289393/1‑148 (MQ=255) aCCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTAG‑CGGGGAAGGCCCCGGCAAAAGG‑GCTCCACGCAATAAccc > 7:125023/1‑148 (MQ=255) cAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCG‑CGGGGAAGCCCGCTCCAACAGG‑GATCCACGCAATCACCtgt < 4:283319/149‑1 (MQ=255) cAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCG‑CGGGGAAGCCCGCTCCAACAGG‑GATCCACGCAATCACCtgt < 7:289393/149‑1 (MQ=255) agaagaGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCG‑CGGGGAAGCCCGCTCCAACAGG‑GATCCACGCAATCACCTGTGGGAGCg < 4:81953/149‑1 (MQ=255) gaagaGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCGCCGGGGAAGCCCGCTCCAACAGG‑GATCCACGCAATCACCTGTGGGAGCg < 1:76920/149‑1 (MQ=255) ggTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCG‑CGGGGAAGCCCGCTCCCACAGGTGATTGCGTGGATC‑CCTGTTGGAGCGGGCTTCCCcgc < 7:240456/149‑1 (MQ=18) gCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTAC‑CGGGGGAGCCCCCACCAACAGG‑GCTCCACGCAATACACGGTGGGAGCGCGGTTGCCcgcg > 2:282242/1‑146 (MQ=255) aaGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCG‑CGGGGGAGACCGCGGCAACAGG‑GATCCACGCAATCCCCGGTGGGAGCGGGCGTCCCCGCGAagcg > 4:67606/1‑147 (MQ=255) gggCCTGGCGACCATGTGGGGGGGTGTCGGCCAAGGTCGGGCGTGGGCCATCGAGCGGGTTTGCCCGGTACCGGCCTCTTCG‑CGGGGAAGCCCGCTCCACCAGG‑GAGCCACGCAATCACCTGGGGGAGCGGGCTTCCCCGCGAAGAGGc < 6:101454/149‑1 (MQ=255) cTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCG‑CGGGGCAGCCCGCTCCAACAGG‑GGTCCCCGCCATCACCTGTGGGCGCGGGCTTGCCCGCGAAGAGGCcgct > 3:215439/1‑149 (MQ=255) cTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCG‑CGGGGAAGCCCGCTCCAACAGG‑GCTCAACGCCATCACCTGTGGGAGCGGGCTTCCCCGCGCAGAGGcagag > 3:219745/1‑145 (MQ=255) gCGACCATGTGTGT‑GGTGTCGGCTAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCG‑CGGGGAAGCCCCCTCCAACAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCtg > 2:105310/1‑149 (MQ=255) gACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCG‑CGGGGAAGCCCGCTCCAACAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGc < 1:101939/140‑1 (MQ=255) gACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCG‑CGGGGAAGCCCGCTCCAACAGG‑GATCCACGCAATAACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGc > 2:101939/1‑140 (MQ=255) aTGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCG‑CGGGGAAGCCCGCTCCAACAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTgaga > 8:101641/1‑149 (MQ=255) tgtgGGTGCCGGCCACGGTCTGGCGTTGCCCATGGCGCGGGTTGCCCCTGTCCCGCCCGCTCCG‑CGGGCACCCCCGCCCCACCCGG‑TACCCCCCCAAACCCCTGGGGGCGGGGGCTCCCCCGCGAAGAGGCCTCTCCTGTGAGACATaa < 4:320491/149‑1 (MQ=255) gggggTGGCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCG‑CGGGGAAGCCCGCTCCAACAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAg < 2:48965/147‑1 (MQ=255) tgGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCG‑CGGGGAAGCCCGCTCCAACAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTcc > 6:207282/1‑117 (MQ=255) tgGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCG‑CGGGGAAGCCCGCTCCAACAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTcc < 5:207282/117‑1 (MQ=255) ggTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCG‑CGGGGAAGCCCGCTCCAACAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGcc > 2:56424/1‑149 (MQ=255) ggCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCG‑CGGGGAAGACCGCTACAACAGG‑GGTGAACGGAATCACCTGTGGGGGCGGGGGTGCCCGCGAAGCGGCCGGGCCGGGGGGACATAAGGGCCATTgg > 7:215170/1‑148 (MQ=255) gCCAAGGTCGGGCGTTGCCCAGCGAGCGGGTATGACCGGTACCGGCCGCTTCG‑CGGGGAAGCCCGCTCCAACCGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGcc < 8:227144/149‑1 (MQ=255) cTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCG‑CGGGGAAGCCCGCGCCAACACG‑GATGCCCCCAATCACCAGGGGGGGCGGGGTTCCCCGCGAAGAGGCCGCGCCGGTGAGAAATAAGGCCCATTgcccgccccct > 8:198466/1‑146 (MQ=255) tGGCGTTGGCCACCGAGCGGGTTGGACCTGTACCGCCCTCTTCG‑CGGGCACGCCCGCTCCAACAGG‑TATCCACGAAATCCCCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATa < 8:297711/149‑1 (MQ=38) gCGTTGCCCATCCAGCGGGTTTGACCTGTACCGGCCTCTTCG‑CGGGGAAGCCCGCTCCAACAGC‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATa < 7:198466/147‑1 (MQ=255) cATCGAGCGGGTTTGACCTGTACCGGCCTCTTCG‑CGGGGAAGCCCGCTACAACAGG‑GATCCACGCAATCACCAGTGGGAGCGGGCTTCCCCCCGAAGAGGCCGATCCGGTGAGACAAAAGGc > 2:261592/1‑122 (MQ=38) aTCGAGCGGGTTTGACCTGTACCGGCCTCTTCG‑CGGGTAAGCCCGCTCCAACAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAgt < 5:137775/149‑1 (MQ=255) aTCGAGCGGGTTTGACCTGTACCGGCCTCTTCG‑CGGGGAAGCCCGCTCCAACAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAgt > 2:285068/1‑149 (MQ=255) aTCGAGCGGGTTTGACCTGTACCGGCCTCTTCG‑CGGGGAAGCCCGCTCCAACAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAgt < 1:285068/149‑1 (MQ=255) gAGCGGGTTTGACCTGTACCGGCCTCTTCG‑CGGGGAAGCCCGCTCCAACAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAgtgtg < 1:105310/149‑1 (MQ=255) tgCGGGTTTGACCTTACCCGGCGTCTTCG‑GGGGCACCCCCGCTCCAACTGG‑GATCCCCGAAATCACCTGTGGGAGGGGGCTGCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAgtgtgt < 1:152654/148‑1 (MQ=255) aGCGGGTTTGACCTGTACCGGCCTCTTCG‑CGGGGAAGCCCGCTCCAACAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAgtgt < 2:234990/147‑1 (MQ=255) aGCGGGTTTGACCTGTACCGGCCTCTTCG‑CGGGGAAGCCCGCTCCAACAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAgtgt > 1:234990/1‑147 (MQ=255) cGGGTTTTACCTGTACCGGCCTCTTCG‑CGGGGAAGCCCGCTCCAACAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTa < 1:282242/149‑1 (MQ=255) aCCTGTACCGGCCTCTTCG‑CGGGGAAGCCCGCTCCAACAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGc < 2:186325/114‑1 (MQ=255) aCCTGTACCGGCCTCTTCG‑CGGGGAAGCCCGCTCCAACAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGc > 1:186325/1‑114 (MQ=255) cGGCCTCTTCG‑CGGGGAAGCCCGCTCCAACAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGaa > 7:283917/1‑149 (MQ=255) tctTCG‑CGGGGAAGCCCGCTCCCACAGGTGATTGCGTGGATC‑CCTGTTGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTAcc > 6:90039/1‑149 (MQ=18) ctTCG‑CGGGGAAGCCCGCTCCAACAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTAcc > 2:253892/1‑148 (MQ=255) cg‑cgGGGAAGCCCGCTCCAACAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAg < 1:253892/148‑1 (MQ=255) cg‑cgGGGAACCCGGCGACAACAGG‑GATCCACCAAATCACCTGTGGCAGCGGGATTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTa < 3:67606/147‑1 (MQ=255) g‑cgGGGAAGCCCGCTCCCACAGGTGATTGCGTGGATC‑CCTGTTGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGa < 5:90039/149‑1 (MQ=18) ggggAAGCCCGCTCCAACAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGAcc > 1:3657/1‑149 (MQ=255) gAAGCCCGCTCCACCAGG‑GCTCCCCCCAATCACCGGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTc < 8:283917/149‑1 (MQ=255) gAAGCCCGCTCCAACAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTc > 8:179580/1‑149 (MQ=255) cccGCTCCAACAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCAg > 5:317799/1‑148 (MQ=255) cAACAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCAGGAGTAAAc > 1:113434/1‑149 (MQ=255) aCAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAg > 1:225933/1‑89 (MQ=255) aCAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAg < 2:225933/89‑1 (MQ=255) aCAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAgt > 8:149998/1‑99 (MQ=255) aCAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAgt < 7:149998/99‑1 (MQ=255) aCAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCAg < 2:31692/139‑1 (MQ=255) aCAGG‑GATCCACGCAATCACCTGTGGGAGCCGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCAg > 1:31692/1‑139 (MQ=255) cACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCAGGAGTAAACCCGTCATGAcc < 7:179580/149‑1 (MQ=255) cACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCAGGAGTAAACCCGTCATGAc < 1:59726/148‑1 (MQ=255) | TGCGTTGCACCAGCTGGAGAAGAGTGGCGGTCGCAAGGGCCTGGCGACCATGTGTGTGGGTGTCGGCCAAGGTCTGGCGTTGGCCATCGAGCGGGTTTGACCTGTACCGGCCTCTTCG‑CGGGGAAGCCCGCTCCAACAGG‑GATCCACGCAATCACCTGTGGGAGCGGGCTTCCCCGCGAAGAGGCCTCTCCTGTGAGACATAAGGCCCATTGCCAGCCCGATAGCGGAACGAGTGTGTTACTAGGTATGTCTAGAACTACCTAGGACCCTCCAGGAGTAAACCCGTCATGACC > NC_002947/1570293‑1570585 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |