Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,581,266 G→T 100% A44D (GCC→GAC)  ttgB ← efflux pump membrane protein TtgB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,581,2660GT96.3% 172.6 / ‑1.1 54A44D (GCC→GAC) ttgBefflux pump membrane protein TtgB
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (26/26);  total (27/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.86e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GGTAATGGTCATGCTGCCGTCGGAGTTACTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGGCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGAAGATCGGGCGATCGATAAAGAACTTCGACATGGTTTACT  >  NC_002947/1581124‑1581404
                                                                                                                                              |                                                                                                                                          
ggTAATCGTCATGCTGCCGTCTGACTTACTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTTCTGCTCGATCACCTGCACCACGGTGTCCTGCACTGTTTGCGCCGAGGCGCCCGGGTAGGTCACGTcgatgtcgatgg                                                                                                                                      <  6:201387/149‑1 (MQ=255)
             ctgcCGTCGGAGTTACTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGa                                                                                                                          >  6:143869/1‑148 (MQ=255)
              tgcCGTCGGAGTTACTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGAt                                                                                                                         >  3:327783/1‑148 (MQ=255)
              tgcCGTCGGAGTTACTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGGCGATGGCTGGCGGCGCGATa                                                                                                                        >  4:151111/1‑149 (MQ=255)
                 cGTCGGAGTTACTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTg                                                                                                                     <  7:7683/149‑1 (MQ=255)
                     ggAGTTACTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTgggg                                                                                                                  <  2:185494/148‑1 (MQ=255)
                      gAGTTACTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGACGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTa                                                                                                                >  1:281958/1‑149 (MQ=255)
                           aCTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTgg                                                                                                            <  3:231416/148‑1 (MQ=255)
                            cTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGtt                                                                                                          <  5:105584/149‑1 (MQ=255)
                            cTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGt                                                                                                           <  4:186839/148‑1 (MQ=255)
                             tttCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGtt                                                                                                          >  5:223251/1‑148 (MQ=255)
                                  gacgacACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCgg                                                                                                    >  5:82777/1‑149 (MQ=255)
                                   acgacACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCgg                                                                                                    >  7:3606/1‑148 (MQ=255)
                                     gacACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAg                                                                                                 >  3:140961/1‑149 (MQ=255)
                                        acaTAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGcgcg                                                                                                                           <  4:309058/120‑1 (MQ=255)
                                        acaTAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGcgcg                                                                                                                           >  3:309058/1‑120 (MQ=255)
                                        acaTAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCtt                                                                                              >  2:312383/1‑149 (MQ=255)
                                        acaTAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGACGAGGCGACCTGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCTATACTGGGGTACTGGTTGATCGGCAGCtt                                                                                              >  5:288577/1‑149 (MQ=255)
                                          aTAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCtt                                                                                              >  5:265533/1‑147 (MQ=255)
                                           tAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAg                                                                                           <  1:312383/149‑1 (MQ=255)
                                            aaCGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAg                                                                                           >  1:20862/1‑148 (MQ=255)
                                              cGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGAt                                                                                        <  5:287402/149‑1 (MQ=255)
                                               gCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATg                                                                                       <  8:3606/149‑1 (MQ=255)
                                                    ttGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGActa                                                                                  <  2:281958/149‑3 (MQ=255)
                                                       tCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGgcgatgtcgatg                                                                                                                                       >  8:177042/1‑93 (MQ=255)
                                                       tCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGgcgatgtcgatg                                                                                                                                       <  7:177042/93‑1 (MQ=255)
                                                              cGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGAcc                                                                        >  7:71619/1‑149 (MQ=255)
                                                               gTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGAc                                                                         >  2:339813/1‑147 (MQ=255)
                                                                   aGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCa                                                                    <  5:104337/148‑1 (MQ=255)
                                                                      tgctgctCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCACGACCAGCATGa                                                                 >  8:119566/1‑148 (MQ=255)
                                                                             cGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAg                                                         <  5:143869/149‑1 (MQ=255)
                                                                                tCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGCCCAGCATGATCACCAAGGc                                                       >  4:103656/1‑148 (MQ=255)
                                                                                 cACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGa                                                     >  3:132958/1‑149 (MQ=255)
                                                                                   ccTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGAt                                                    >  1:296857/1‑148 (MQ=255)
                                                                                   ccTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATc                                                   <  1:339813/149‑1 (MQ=255)
                                                                                   ccTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATc                                                   >  7:66141/1‑149 (MQ=255)
                                                                                   ccTGCAACACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCGGGGGAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATc                                                   <  8:71619/149‑1 (MQ=255)
                                                                                    cTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATcg                                                  >  5:238730/1‑148 (MQ=255)
                                                                                    cTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGGCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCa                                                  <  3:151111/149‑1 (MQ=255)
                                                                                      gCACCACGGTGTCCTGCACGGTTTGTGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCAcc                                                >  4:222906/1‑149 (MQ=255)
                                                                                       caccacGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCAc                                                 <  4:140961/147‑1 (MQ=255)
                                                                                           acGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGc                                           <  4:132958/149‑1 (MQ=255)
                                                                                            cGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGAc                                                                         <  7:157585/118‑1 (MQ=255)
                                                                                            cGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGAc                                                                         >  8:157585/1‑118 (MQ=255)
                                                                                                   cTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATTATCACCAAGGCGATCACCCAGGCGAAGATCg                                   <  6:223251/149‑1 (MQ=255)
                                                                                                     gCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGAAGATCggg                                 >  6:296462/1‑149 (MQ=255)
                                                                                                      cACGGTTTGCGGCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGAAGATCgg                                  <  6:82777/147‑1 (MQ=255)
                                                                                                           tttGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGAAGATCGGGcgatc                            >  2:127568/1‑148 (MQ=255)
                                                                                                               cgcCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGAAGATCGGGCGATCGATaa                       <  8:39680/149‑1 (MQ=255)
                                                                                                               cgcCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGAAGATCGGGCGATCGATaa                       <  8:44833/149‑1 (MQ=255)
                                                                                                                gcCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCGGCATGATCACCAAGGCGATCACCCAGGCGAAGATCGGGCGATCGATa                        >  3:21273/1‑147 (MQ=255)
                                                                                                                 ccGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGAAGATCGGGCGATCGATAAAg                     <  7:119566/149‑1 (MQ=255)
                                                                                                                  cGAGGCGCCCGGGTAGGTCACGTCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGAAGATCGGGCGATCGATAAAGa                    <  2:296857/149‑1 (MQ=255)
                                                                                                                   gAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGCGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACAAAGGCTATCACCCAGGCGAAGATCGGGCGATCTATaaaca                    >  4:212650/1‑146 (MQ=255)
                                                                                                                    aGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGAAGATCGGGCGATCGATAAAGAAc                  <  2:20862/149‑1 (MQ=255)
                                                                                                                         cccGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGAAGATCGGGCGATCGATAAAGAACTTCGa             <  4:327783/149‑1 (MQ=255)
                                                                                                                                     aCGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGAAGATCGGGCGATCGATAAAGAACTTCGACATGGTTTACt  <  6:265533/148‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                          
GGTAATGGTCATGCTGCCGTCGGAGTTACTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGGCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGAAGATCGGGCGATCGATAAAGAACTTCGACATGGTTTACT  >  NC_002947/1581124‑1581404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑