Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,591,249 C→T 7.7% P215P (CCG→CCA)  PP_1395 ← AraC family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,591,2490CT 7.7% 144.5 / 2.3 52P215P (CCG→CCA) PP_1395AraC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (23/24);  new base T (2/2);  total (25/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.97e-01

GTCGTTCAACATCAGCCGCCGCGCCTCCTGCAGGCGCAGCTGCTTCTGGTACTGAAGCGGGCTCATCGAGGTCACGGCCTTGAAGCGGTGGTGCAAGGTCGAGGTGCTGAGGTTGACCTCAC‑GGGCCAGGTCCTCGATACGCAGCGGTTGTTGG‑AAGTTGTTGTTGAGCCAAGTGATGGCCTGGCAAACCCGGTGGGTCTGGCTGTTGGCCAAGGCAATTTCATACAGCCGGTAACCCTGCGGCCCACGCAGCAGGCGGTAGAGAATCTCGCGGCGGAACAGCGGCGC  >  NC_002947/1591105‑1591392
                                                                                                                                                 |                                                                                                                                                
gtcgtTCAACATCAGCCGCCGCGCCTCCTGCAGGCGCAGCTGCTTCTGGTACTGACGCGGGCTCATCGAGGTCACGGCCGTGAAGCGGTGGTGCAAGGTCGAGGTGCTGAGGTTGACCTCAC‑GGGCCAGGTCCTCGATACGCAGCGgtt                                                                                                                                              <  6:69973/149‑1 (MQ=255)
gtcgtTCAACATCAGCCGCCGCGCCTCCTGCAGGCGCAGCTGCTTCTGGTACTGAAGCGGGCTCATCGAGGTCACGGCCTTGAAGCGGTGGTGCAAGGTCGAGGTGCTGAGGTTGACCTCAC‑GGGCCAGGTCCTCGATACGCAGCGgtt                                                                                                                                              >  5:181093/1‑149 (MQ=255)
    ttCAACATCAGCCGCCGCGCCTCCTGCAGGCGCAGCTGCTTCTGGTACTGAAGCGGGCTCATCGAGGTCACGGCCTTGAAGCGGTGGTGCAAGGTCGAGGTGCTGAGGTTGACCTCAC‑GGGCCAGGTCCTCGATACGCAGCGGttgttg                                                                                                                                          <  2:69953/149‑1 (MQ=255)
        aCATCAGCCGCCGCGCCTCCTGCAGGCGCAGCTGCTTCTGGTACTGAAGCGGGCTCATCGAGGTCACGGCCTTGAAGCGGTGGTGCAAGGTCGAGGTGCTGAGGTTGACCTCAC‑GGGCCAGGTCCTCGATACGCAGCGGTTGTTGG‑AAg                                                                                                                                     <  6:181093/149‑1 (MQ=255)
         cagaagcagcTGCGCCTGCAGGAGGCGCGGCTGCTTCTGGTACTGAAGCGGGCTCATCGAGGTCACGGCCTTGAAGCGGTGGTGCAAGGTCGAGGTGCTGAGGTTGACCTCAC‑GGGCCAGGTCCTCGATACGCAGCGGTTGTTGG‑AAgt                                                                                                                                    >  5:133941/5‑149 (MQ=255)
          aTCAGCCGCCGCGCCTCCTGCAGGCGCAGCTGCTTCTGGTACTGAAGCGGGCTCATCGAGGTCACGGCCTTGAAGCGGTGGTGCAAGGTCGAGGTGCTGAGGTTGACCTCAC‑GGGCCAGGTCCTCGATACGCAGCGGTTGTTGG‑AAgtt                                                                                                                                   >  3:222368/1‑149 (MQ=255)
          aTCAGCCGCCGCGCCTCCTGCAGGCGCAGCTGCTTCTGGTACTGAAGCGGGCTCATCGAGGTCACGGCCTTGAAGCGGTGGTGCAAGGTCGAGGTGCTGAGGTTGACCTCAC‑GGGCCAGGTCCTCGATACGCAGCGGTTGTTGG‑AAgtt                                                                                                                                   >  7:10039/1‑149 (MQ=255)
          aTCAGCCGCCGCGCCTCCTGCAGGCGCAGCTGCTTCTGGTACTGAAGCGGGCTCATCGAGGTCACGGCCTTGAAGCGGTGGTGCAAGGTCGAGGTGCTGAGGTTGACCTCAC‑GGGCCAGGTCCTCGATACGCAGCGGTTGTTGG‑AAgtt                                                                                                                                   <  8:270461/149‑1 (MQ=255)
               ccgccgCGCCTCCTGCAGGCGCAGCTGCTTCTGGTACTGAAGCGGGCTCATCGAGGTCACGGCCTTGAAGCGGTGGTGCAAGGTCGAGGTGCTGAGGTTGACCTCAC‑GGGCCAGGTCCTCGATACGCAGCGGTTGTTGG‑AAgttgttgt                                                                                                                              >  5:153036/1‑149 (MQ=255)
                cgccgcGCCTCCTGCAGGCGCAGCTGCTTCTGGTACTGAAGCGGGCTCATCGAGGTCACGGCCTTGAAGCGGTGGTGCAAGGTCGAGGTGCTGAGGTTGACCTCAC‑GGGCCAGGTCCTCGATACGCAGCGGTTGTTGG‑AAgttgttgtt                                                                                                                             <  1:124842/149‑1 (MQ=255)
                cgccgcGCCTCCTGCAGGCGCAGCTGCTTCTGGTACTGAAGCGGGCTCATCGAGGTCACGGCCTTGAAGCGGTGGTGCAAGGTCGAGGTGCTGAGGTTGACCTCAC‑GGGCCAGGTCCTCGATACGCAGCGGTTGTTGG‑AAgttgttgtt                                                                                                                             <  2:220108/149‑1 (MQ=255)
                     cgcCTGCAGGAGGCGCGGCTGCTTCTGGTACTGAAGCGGGCTCATCGAGGTCACGGCCTTGAAGCGGTGGTGCAAGGTCGAGGTGCTGAGGTTGACCTCAC‑GGGCCAGGTCCTCGATACGCAGCGGTTGTTGG‑AAGTTGTTGTTGAg                                                                                                                          <  6:133941/147‑1 (MQ=255)
                        ctcctGCAGGCGCAGCTGCTTCTGGTACTGAAGCGGGCTCATCGAGGTCACGGCCTTGAAGCGGTGGTGCAAGGTCGAGGTGCTGAGGTTGACCTCAC‑GGGCCAGGTCCTCGATACGCAGCGGTTGTTGG‑AAGTTGTTGTTGAGCCAAg                                                                                                                     >  5:324022/1‑149 (MQ=255)
                              cAGGCGCAGCTGCTTCTGGTACTGAAGCGGGCTCATCGAGGTCACGGCCTTGAAGCGGTGGTGCAAGGTCGAGGTGCTGAGGTTGACCTCAC‑GGGCCAGGTCCTCGATACGCAGCGGTTGTTGG‑AAGTTGTTGTTGAGCCAAGTGATg                                                                                                                <  6:153036/148‑1 (MQ=255)
                               aGGCGCAGCTGCTTCTGGTACTGAAGCGGGCTCATCGAGGTCACGGCCTTGAAGCGGTGGTGCAAGGTCGAGGTGCTGAGGTTGACCTCAC‑GGGCCAGGTCCTCGATACGCAGCGGTTGTTGG‑AAGTTGTTGTTGAGCCAAGTGATGGc                                                                                                              <  4:222368/149‑1 (MQ=255)
                                 gcgcAGCTGCTTCTGGTACTGAAGCGGGCTCATCGAGGTCACGGCCTTGAAGCGGTGGTGCAAGGTCGAGGTGCTGAGGTTGACCTCAC‑GGGCCAGGTCCTCGATACGCAGTGGTTGTTGG‑AAGTTGTTGTTGAGCCAAGTGATGGCCt                                                                                                            >  2:213955/1‑149 (MQ=255)
                                         gctTCTGGTACTGAAGCGGGCTCATCGAGGTCACGGCCTTGAAGCGGTGGTGCAAGGTCGAGGTGCTGAGGTTGACCTCAC‑GGGCCAGGTCCTCGATACGCAGTGGTTGTTGG‑AAGTTGTTGTTGAGCCAAGTGATGGCCTGGCAAAcc                                                                                                    >  4:94097/1‑149 (MQ=255)
                                             cTGGAACTGAAGCGGGCTCATCGAGGTCACGGCATAGAAGCGGTGGTGCAAGGTCGAGGTGCTGAGGTTGACCTCAC‑GGGCCAGGTCCTCCATACGCACCGGTTGTTGG‑AATTTGTTGTTGAGCCAAGTGATGGCAGAGCAATCCCgtt                                                                                                >  2:260547/1‑147 (MQ=255)
                                                gTACTGAAGCGGTCTCATCGAGGGCACGGCCTTGAAGCGGTGGTGCAATGTCGAGGTGCTGAGGTTGACCTCAC‑GGGCCAGGTCCTCGATACGCAGCGGTTGTTGG‑AAGTTGTTGTTGAGCCAAGTGATGGCCTGGCAAACCCGGt                                                                                                <  2:83251/146‑1 (MQ=255)
                                                    tGAAGCGGGCTCATCGAGGTCACGGCCTTGAAGCGGTGGTGCAAGGTCGAGGTGCTGAGGTTGACCTCAC‑GGGCCAGGTCCTCGATACGCAGCGGTTGTTGG‑AAGTTGTTGTTGAGCCAAGTGATGGCCTCGCAAACCCGGTCGGTCTg                                                                                         >  7:86636/1‑149 (MQ=255)
                                                          gggCGCATCGAGGCCACGCCCTCCACCCGGGGGTGCACGGCCGAGGTGCTGAGGTTGACCTCAC‑GGGCCAGGTCCTCCTTACCCCGCGGTTGTTGC‑ACGTTGTTGTGCAGCCACGTGATGGCCTGGCAAACCCGGTGGGTCTGGCTGtt                                                                                   <  6:324022/149‑1 (MQ=255)
                                                           ggCTCATCGAGGTCACGGCCTTGAAGCGGTGGTGCAAGGTCGAGGTGCTGAGGTTGACCTCAC‑GGGCCAGGTCCTCGATACGCAGTGGTTGTTGG‑AAGTTGTTGTTGAGCCAAGTGATGGCCTGGCAAACCCGGTGGGTCTGGCTGTTg                                                                                  <  3:94097/149‑1 (MQ=255)
                                                            gCTCATCGAGGTCACGGCCTTGAAGCGGTGGTGCAAGGTCGAGGTGCTGAGGTTGACCTCAC‑GGGCCAGGTCCTCGATACGCAGCGGTTGTTGG‑AAGTTGTTGTTGAGCCAAGTGATGGCCTGGCAAAcc                                                                                                    >  1:125719/1‑130 (MQ=255)
                                                            gCTCATCGAGGTCACGGCCTTGAAGCGGTGGTGCAAGGTCGAGGTGCTGAGGTTGACCTCAC‑GGGCCAGGTCCTCGATACGCAGCGGTTGTTGG‑AAGTTGTTGTTGAGCCAAGTGATGGCCTGGCAAAcc                                                                                                    <  2:125719/130‑1 (MQ=255)
                                                                aTCGAGGTCACGGCCTTGAAGCGGTGGTGCAAGGTCGAGGTGCTGAGGTTGACCTCAC‑GGGCCAGGTCCTCGATACGCAGCGGTTGTTGG‑AAGTTGTTGTTGAGCCAAGTGATGGCCTGGCAAACCCGGTGGGTCTGGCTGTTGGCCaa                                                                             <  7:234513/149‑1 (MQ=255)
                                                                aTCGAGGTCACGGCCTTGAAGCGGTGGTGCAAGGTCGAGGTGCTGAGGTTGACCTCAC‑GGGCCAGGTCCTCGATACGCAGCGGTTGTTGG‑AAGTTGTTGTTGACCCAAGTGATGGCCTGGCAAACCCGGTGGGTCTGGCTGTTGGCCaa                                                                             <  1:243778/149‑1 (MQ=255)
                                                                 tCGAGGTCACGGCCTTGAAGCGGTGGTGCAAGGTCGAGGTGCTGAGGTTGACCTCAC‑GGGCCAGGTCCTCGATACGCAGTGGTTGTTGG‑AAGTTGTTGTTGAGCCAAGTGATGGCCTGGCAAACCCGGTGGGTCTGGCTGTTGGCCAAg                                                                            <  1:213955/149‑1 (MQ=255)
                                                                 tCGAGGTCACGGCCTTGAAGCGGTGGTGCAAGGTCGAGGTGCTGAGGTTGACCTCAC‑GGGCCAGGTCCTCGATACGCAGCGGTTGTTGG‑AAGTTGTTGTTGAGCCAAGTGATGGCCTGGCAAACCCGGTgg                                                                                              >  4:200919/1‑131 (MQ=255)
                                                                 tCGAGGTCACGGCCTTGAAGCGGTGGTGCAAGGTCGAGGTGCTGAGGTTGACCTCAC‑GGGCCAGGTCCTCGATACGCAGCGGTTGTTGG‑AAGTTGTTGTTGAGCCAAGTGATGGCCTGGCAAACCCGGTgg                                                                                              <  3:200919/131‑1 (MQ=255)
                                                                 tCGAGGTCACGGCCTTGAAGCGGTGGTGCAAGGTCGAGGTGCTGAGGTTGACCTCAC‑GGGCCAGGTCCTCGATACGCAGCGGTTGTTGG‑AAGTTGTTGTTGAGCCAAGTGATGGCCTGGCAAACCCGGTGGGTCTGGCTGTTGGCCAAg                                                                            >  1:153541/1‑149 (MQ=255)
                                                                   gAGGTCACGGCCTTGAAGCGGTGGTGCAAGGTCGAGGTGCTGAGGTTGACCTCAC‑GGGCCAGGTCCTCGATACGCAGCGGTTGTTGG‑AAGTTGTTGTTGAGCCAAGTGATGGCCTGGCAAACCCGGTGGGTCTGGCTGTTGGCCAAgg                                                                           <  8:86636/148‑1 (MQ=255)
                                                                         aCGGCCTTGAAGCGGTGGTGCAAGGTCGAGGTGCTGAGGTTGACCTCAC‑GGGCCAGGTCCTCGATACGCAGCGGTTGTTGG‑AAGTTGTTGTTGAGCCAAGTGATGGCCTGGCAAACCCGGTGGGTCTGGCTGTTGGCCAAGGCAATTTc                                                                    <  5:122423/149‑1 (MQ=255)
                                                                                       gtggtgCAAGGTCGAGGTGCTGAGGTTGACCTCAC‑GGGCCAGGTCCTCGATACGCAGCGGTTGTTCG‑AAGTTGTTGTTGAGCCAAGTGATGGCCTGGCAAACCCGGTGGGTCTGGCTGTTGGCCAAGGCAATTTCATACAGCCGGTAAc                                                      >  5:239194/1‑149 (MQ=255)
                                                                                        tggtgCAAGGTCGAGGTGCTGAGGTTGACCTCAC‑GGGCCAGGTCCTCGATACGCAGCGGTTGTTGG‑ATGTTGTTGTTGAGCCAAGTGATGGCCTTTCAAACCCGGTGGGTCTGGCTGTTGGCCAAGGCAATTTCATACAGCCGGTAAcc                                                     <  1:260547/149‑1 (MQ=255)
                                                                                        tggtgCAAGGTCGAGGTGCTGAGGTTGACCTCAC‑GGGCCAGGTCCTCGATACGCAGCGGTTGTTGG‑AAGTTGTTGTTGAGCCAAGTGATGGCCTGGCAAACCCGGTGGGt                                                                                            <  5:325591/110‑1 (MQ=255)
                                                                                        tggtgCAAGGTCGAGGTGCTGAGGTTGACCTCAC‑GGGCCAGGTCCTCGATACGCAGCGGTTGTTGG‑AAGTTGTTGTTGAGCCAAGTGATGGCCTGGCAAACCCGGTGGGt                                                                                            >  6:325591/1‑110 (MQ=255)
                                                                                           tgCAAGGTCGAGGTGCTGAGGTTGACCTCAC‑GGGCCAGGTCCTCGATACGCAGCGGTTGTTGG‑AAGTTGTTGTTGAGCCAAGTGATGGCCTGGCAAACCCGGTGGGTCTGGCTGTTGGCCAAGGCAATTTCATACAGcc                                                            <  2:71446/139‑1 (MQ=255)
                                                                                           tgCAAGGTCGAGGTGCTGAGGTTGACCTCAC‑GGGCCAGGTCCTCAATACGCAGCGGTTGTTGG‑AAGTTGTTGTTGAGCCAAGTGATGGCCTGGCAAACCCGGTGGGTCTGGCTGTTGGCCAAGGCAATTTCATACAGcc                                                            >  1:71446/1‑139 (MQ=255)
                                                                                             cAAGGTCGAGGTGCTGAGGTTGACCTCAC‑GGGCCAGGTCCTCGATACGCAGCGGTTGTTGGAAAATTGTTGTTGAGCCAAGTGATGGCATGGCAAACCCGTTGGGTCTGGATGTTGGCCAAGGAAATTTCATACTGCCGGTAACCATGc                                                 >  1:275344/1‑149 (MQ=255)
                                                                                               aGGTCGAGGTGCTGAGGTTGACCTCACGGGGCCAGGTCCTCGATACGCAGCGGTTGTTGG‑AAGTTGTTGTTGAGCCAAGTGATGGCCTGGCAAACCCGGTGGGTCTGGCTGTTGGCCAAGGCAATTTCATACAGCCGGTAACCCTGCgg                                               >  1:196180/1‑149 (MQ=255)
                                                                                                ggTCGAGGTGCTGAGGTTGACCTCAC‑GGGCCAGGTCCTCGATACGCAGCGGTTGTTGG‑AAGTTGTTGTTGAGCCAAGTGATGGCCTGGCAAACCCGGTGGGTCTGGCTGTTGGCCAAGGCAATTTCATACAGCCGGTAACCCTGCGGcc                                             >  6:57524/1‑149 (MQ=255)
                                                                                                       gTGCTGAGGTTGACCTCAC‑GGGCCAGGTCCTCGATACGCAGCGGTTGTTGG‑AAGTTGTTGTTGAGCCAAGTGATGGCCTGGCAAACCCGGTGGGTCTGGCTGTTGGCCAAGGCAATTTCATACAGCCGGTAACCCTGCGGCCCACgcag                                      <  5:57524/149‑1 (MQ=255)
                                                                                                            gAGGTTGACCTCAC‑GGGCCAGGTCCTCGATACGCAGCGGTTGTTGG‑AAGTTGTTGTTGAGCCAAGTGATGGCCTGGCAAACCCGGTGGGTCTGGCTGTTGGCCAAGGCAATTTCATACAGCCGGTAACCCTGCGGCCCACGCAGCAGGc                                 >  5:72410/1‑149 (MQ=255)
                                                                                                                  gACCTCAC‑GGGCCAGGTCCTCGATACGCAGCGGTTGTTGG‑AAGTTGTTGTTGAGCCAAGTGATGGCCTGGCAAACCCGGTGGGTCTGGCTGTTGGCCAAGGCAATTTCATACAGCCGGTAACCCTGCGGCCCACGCAGCAGGCGGTag                            <  2:153541/148‑1 (MQ=255)
                                                                                                                   aCCTCAC‑GGGCCAGGTCCTCGATACGCAGCGGTTGTTGG‑AAGTTGTTGTTGAGCCAAGTGATGGCCTGGCAAACCCGGTGGGTCTGGCTGTTGGCCAAGGCAATTTCATACAGCCGGTAACCCTGCg                                                >  6:167189/1‑127 (MQ=255)
                                                                                                                   aCCTCAC‑GGGCCAGGTCCTCGATACGCAGCGGTTGTTGG‑AAGTTGTTGTTGAGCCAAGTGATGGCCTGGCAAACCCGGTGGGTCTGGCTGTTGGCCAAGGCAATTTCATACAGCCGGTAACCCTGCg                                                <  5:167189/127‑1 (MQ=255)
                                                                                                                    ccTCAC‑GGGCCAGGTCCTCGATACGCAGCGGTTGTTGG‑AAGTTGTTGTTGAGCCAAGTGATGGCCTGGCAAACCCGGTGGGTCTGGCTGTTGGCCAAGGCAATTTCATACAGCCGGTAACCCTGCGGCCCACGCAGCAGGCGGTagag                          >  6:117217/1‑148 (MQ=255)
                                                                                                                    ccTCAC‑GGGCCAGGTCCTCGATACGCAGCGGTTGTTGG‑AAGTTGTTGTTGAGCCAAGTGATGGCCTGGCAAACCCGGTGGGTCTGGCTGTTGGCCAAGGCAATTTCATACAGCCGGTAACCCTGCGGCCCACGCAGCAGGCGGTagag                          >  2:152351/1‑148 (MQ=255)
                                                                                                                        aC‑GGGCCAGGTCCTCGATACGCAGCGGTTGTTGG‑AAGTTGTTGTTGAGCCAAGTGATGGCCTGGCAAACCCGGTGGGTCTGGCTGTTGGCCAAGGCAATTTCATACAGCCGGTAACCCTGCGGCCCACGCAGCAGGCGGTAGAGAAtct                     >  1:278290/1‑149 (MQ=255)
                                                                                                                             gCCAGGTCCTCGATACGCAGCGGTTGTTGG‑AAGTTGTTGTTGAGCCAAGTGATGGCCTGGCAAACCCGGTGGGTCTGGCTGTTGGCCAAGGCAATTTCATACAGCCGGTAACCCTGCGGCCCACGCAGCAGGCGGTAGAGAATCTcgcg                 <  2:275344/149‑1 (MQ=255)
                                                                                                                                           actcAGAGGTTGTTGG‑AAGTTGTTGTTGAGCCAAGTGATGGCCTGGCAAACCCGGTGGGTCTGGCTGTTGGCCAAGGCAATTTCATACAGCCGGTAACCCTGCGGCCCACGCAGCAGGCGGTAGAGAATCTCGCGGCGGAACAgcggc    <  6:239194/145‑1 (MQ=255)
                                                                                                                                              cAGCGGTTGTTGG‑AAGTTGTTGTTGAGCCAAGTGATGGCCTGGCAAACCCGGTGGGTCTGGCTGTTGGCCAAGGCAATTTCATACAGCCGGTAACCCTGCGGCCCACGCAGCAGGCGGTAGAGAATCTCGCGGCGGAACAGCGgcgc  >  2:192043/1‑147 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GTCGTTCAACATCAGCCGCCGCGCCTCCTGCAGGCGCAGCTGCTTCTGGTACTGAAGCGGGCTCATCGAGGTCACGGCCTTGAAGCGGTGGTGCAAGGTCGAGGTGCTGAGGTTGACCTCAC‑GGGCCAGGTCCTCGATACGCAGCGGTTGTTGG‑AAGTTGTTGTTGAGCCAAGTGATGGCCTGGCAAACCCGGTGGGTCTGGCTGTTGGCCAAGGCAATTTCATACAGCCGGTAACCCTGCGGCCCACGCAGCAGGCGGTAGAGAATCTCGCGGCGGAACAGCGGCGC  >  NC_002947/1591105‑1591392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑