Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,639,596 C→T 11.6% intergenic (‑186/‑125) czcR‑III ← / → PP_1439 response regulator/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,639,5960CT11.6% 107.3 / 5.1 43intergenic (‑186/‑125)czcR‑III/PP_1439response regulator/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (22/16);  new base T (3/2);  total (25/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.01e-01

TGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCG‑CCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGGCAAACACGATGAACCTGACCAAATACCTGCTG  >  NC_002947/1639455‑1639744
                                                                                                                                             |                                                                                                                                                     
tGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCcgcg                                                                                                                                                   >  4:233507/1‑146 (MQ=255)
               gcgcCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCATAGCGAGTTAAACCGCGCCTGTAGGCACCGGTTTACCCGCGGAGCGGCCCGAACAGtg                                                                                                                                  >  4:174590/1‑147 (MQ=255)
                 gcCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGCATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCcac                                                                                                                               <  7:247805/149‑1 (MQ=255)
                  ccccAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGTAGAGGCCCGCAGAGTcccc                                                                                                                               >  8:247805/1‑146 (MQ=255)
                   cccAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCcaca                                                                                                                              >  2:230857/1‑148 (MQ=255)
                      aaTGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAg                                                                                                                          <  3:233507/149‑1 (MQ=255)
                              ggCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGTGCCTCTTCGCGAGTAAACCAGCGCCTCTAGGAACGGGTTTACCAGCGAAGAGGCCCGAACAGTCCACACAAGGcccaatt                                                                                                                  >  4:199149/1‑144 (MQ=255)
                                 aTGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTg                                                                                                                 >  5:8523/1‑147 (MQ=255)
                                  tGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCTAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAg                                                                                                              <  4:118040/149‑1 (MQ=255)
                                              tGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATccc                                                                                                  >  7:303921/1‑149 (MQ=255)
                                                 ggTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCTCG‑c                                                                                              >  2:78927/1‑149 (MQ=255)
                                                 ggTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCG‑c                                                                                              <  6:99515/149‑1 (MQ=255)
                                                      aGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCG‑CCTCAc                                                                                         <  7:260049/149‑1 (MQ=255)
                                                            cGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCTCG‑CCTCACCTTGcc                                                                                   <  1:78927/149‑1 (MQ=255)
                                                             ggATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCAGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCG‑CCTCACCTTGCCg                                                                                  >  2:66275/1‑149 (MQ=255)
                                                                           gctgTTATCTGGCTGTCTGTTCGGGCCTCTTAGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCG‑CCTCACCTTGCCGTTAGCCAGCCCTCg                                                                    >  3:9113/1‑149 (MQ=255)
                                                                             tgTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCG‑CCTCACCTTGCCGTTAGCCAGCCCTCGc                                                                   >  4:24277/1‑148 (MQ=255)
                                                                                 acgtGGCTGTGTGGTCGGCCCTCTGCGCGAGTAACCCCGCGCCTGTAGCAACGGTTTTACCCGCGATGCGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCG‑CCTCACCTTGCCGTTAGCCAGCCCTc                                                                     <  4:321806/139‑1 (MQ=255)
                                                                                 aTCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCCCACAAGGCCACATTGCAGGACTGTAATCCCCG‑CCTCCCCTTGCCGTTAGCCAGCCCTc                                                                     >  3:321806/1‑142 (MQ=255)
                                                                                 aTCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCG‑CCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGa                                                              >  8:140818/1‑149 (MQ=255)
                                                                                 aTCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCG‑CCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGa                                                              >  2:74672/1‑149 (MQ=255)
                                                                                      gCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCGCCCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGc                                                          <  1:230857/149‑1 (MQ=255)
                                                                                              ttCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCG‑CCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCt                                                   <  1:66275/147‑1 (MQ=255)
                                                                                              ttCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCG‑CCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCt                                                   <  7:140818/147‑1 (MQ=255)
                                                                                                 gggCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCG‑CCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATc                                               <  1:74672/148‑1 (MQ=255)
                                                                                                  ggCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCG‑CCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCgg                                             <  3:24277/149‑1 (MQ=255)
                                                                                                   gCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGGAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCG‑CCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGt                                            >  8:183852/1‑149 (MQ=255)
                                                                                                    ccTCTTCGCGATAAAACCCGCGCCTGTAGGAACGGTTTTACCCGCTAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCG‑CCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGtt                                           <  4:9113/149‑1 (MQ=255)
                                                                                                        ttCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCG‑CCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGttatta                                       >  8:194069/1‑149 (MQ=255)
                                                                                                          cgcgGGTAAACCCGTTCCTACAGGCGCGGGTTTACTCGCGAAGAGGCCCGAACAGACCACACAAGGCCACATTGCAGGACTGTAATCCCCG‑CCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTAc                                      >  3:49954/1‑148 (MQ=25)
                                                                                                           gcgGGTAAACCCGTTCCTACAGGCGCGGGTTTACTCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCCGGACTGTAATCCCCG‑CCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTAc                                      >  6:352012/1‑147 (MQ=25)
                                                                                                           gcgGGTAAACCCGTTCCTACAGGCGCGGGTTTACTCGCGAAGAGGCCCGAACAGACCACACAAGGCCACATTGCAGGACTGTAATCCCCG‑CCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGa                                    <  4:49954/149‑1 (MQ=25)
                                                                                                            cgAGTAAACCCGCGCCTGTAGGATCGGTTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCG‑CCTCACCTTGCGGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAg                                   <  2:40657/149‑1 (MQ=255)
                                                                                                             gggTAAACCCGTTCCTACAGGCGCGGGTTTACTCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCG‑CCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAgg                                  >  2:285015/3‑149 (MQ=37)
                                                                                                                    cccGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCG‑CCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGGCAAacac                           >  4:17214/1‑149 (MQ=255)
                                                                                                                    cccGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCG‑CCTCACCTTGCCGTTAGCCAGCCCGCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGGCAAtcac                           >  7:146340/1‑149 (MQ=255)
                                                                                                                      cgcgCCTGTAGGAACGGGTTTACCCGCGGAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCG‑CCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGGCAAACACGa                         <  7:183852/149‑1 (MQ=255)
                                                                                                                      cgcgCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCG‑CCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGGCAAACACGa                         >  4:113458/1‑149 (MQ=255)
                                                                                                                           cTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCG‑CCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGGCAAACACGa                         >  6:43626/1‑144 (MQ=255)
                                                                                                                           cTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCG‑CCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGGCAAACACGa                         <  5:43626/144‑1 (MQ=255)
                                                                                                                             gTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCG‑CCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGGCAAACACGATGAACCt                  >  4:127129/1‑149 (MQ=255)
                                                                                                                                    cGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCG‑CCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGGCAAACACGATGAACCTGACCaaa           >  1:207721/1‑149 (MQ=255)
                                                                                                                                        tttACTCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCG‑CCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGGCAAACACGATGAACCTGACCAAATAcc       <  1:285015/149‑1 (MQ=255)
                                                                                                                                             ccGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCG‑CCTCCCCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGGCAAACACGATGAACCTGACCAAATACctgctg  >  2:213641/1‑149 (MQ=255)
                                                                                                                                             ccGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCG‑CCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGGCAAACACGATGAACCTGACCAAATAcc       >  8:144702/1‑144 (MQ=255)
                                                                                                                                             ccGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCG‑CCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGGCAAACACGATGAACCTGACCAAATAcc       <  7:144702/144‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                     
TGACATCAGGGTCAAGCGCCCCAATGTTACGGCATGAAAAAAGGCCTGCGGTGCAGATAACGGATTTGTAATTCTGCTGTTATCTGGCTGTCTGTTCGGGCCTCTTCGCGAGTAAACCCGCGCCTGTAGGAACGGGTTTACCCGCGAAGAGGCCCGAACAGTCCACACAAGGCCACATTGCAGGACTGTAATCCCCG‑CCTCACCTTGCCGTTAGCCAGCCCTCGCTAGGATGGCCGTGTCTGATCGGTTATTACGAGGCAAACACGATGAACCTGACCAAATACCTGCTG  >  NC_002947/1639455‑1639744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑