Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,684,272	T→C	9.3%	F284S (TTT→TCT)	PP_1478 →	xenobiotic reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,684,272	0	T	C	9.3%	179.9 / 7.7	68	F284S (TTT→TCT)	PP_1478	xenobiotic reductase
Reads supporting (aligned to +/- strand):  ref base T (28/32);  new base C (2/4);  total (30/38)
Fisher's exact test for biased strand distribution p-value = 6.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

ACTGGGTCGAGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCGGGAGCTGCTGCGCGATCCTTACTGG  >  NC_002947/1684125‑1684417                                                                                                                                                    |                                                                                                                                                  actgGGTCGAGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCttt                                                                                                                                                  >  8:230894/1‑149 (MQ=255) actgGGTCGAGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCttt                                                                                                                                                  >  4:133957/1‑149 (MQ=255)     ggTCGAGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGc                                                                                                                                                <  7:230894/147‑1 (MQ=255)     ggTCGAGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGc                                                                                                                                                <  7:225652/147‑1 (MQ=255)     ggTCGAGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGc                                                                                                                                                <  3:133957/147‑1 (MQ=255)          aGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGAGCTTTGCAGAgcgc                                                                                                                                         >  8:294007/1‑149 (MQ=255)              cGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTgc                                                                                                                                     <  2:297147/149‑1 (MQ=255)              cGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTCTGCAAAGCGCGtct                                                                                                                                     <  1:227735/149‑3 (MQ=255)                  tGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTCTGCAAAGCGCGtctgata                                                                                                                                 <  2:271716/149‑7 (MQ=255)                    gAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCaa                                                                                                                                 <  3:140730/147‑1 (MQ=255)                    gAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTCTGCAAAGCGCGtctgata                                                                                                                                 <  5:27797/147‑7 (MQ=255)                     aaCCCGGATGAAACCGTCGAACTGGATCGTCGCCTGCGGGTGATGGGTGTCGACCTGATCGATGTGTCATCCGGTGGTACCTCGGTCAAAGCCGAAATACCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGATCGCGTGAGCAAgg                                                                                                                               >  6:21286/1‑148 (MQ=255)                         cGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCa                                                                                                                          <  5:210434/149‑1 (MQ=255)                          ggATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTTTCCTCCGGGGGTACCTCGGTCAACGCCGAAATTCCTACAGTCCCTGTCTATCAGACGCTCTATGCAGAGCGCGTGCGCAAGGAAGCag                                                                                                                         <  5:21286/149‑1 (MQ=255)                             tGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAgaga                                                                                                                      <  5:185624/149‑1 (MQ=255)                                       gAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCgg                                                                                                            <  4:285369/149‑1 (MQ=255)                                           tGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCAcc                                                                                                        >  6:88197/1‑149 (MQ=255)                                             gCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGt                                                                                                      >  2:254492/1‑149 (MQ=255)                                                 gtcgCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCgg                                                                                                   >  4:99911/1‑148 (MQ=255)                                                 gtcgCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCg                                                                                                    <  5:196952/147‑1 (MQ=255)                                                         cGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCa                                                                                           <  8:316562/148‑1 (MQ=255)                                                           ggTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGTGATCGCCACCGGCACCGTCGGCATGATCACTg                                                                                        <  1:42205/149‑1 (MQ=255)                                                                   gtgtCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGcc                                                                                >  2:239766/1‑149 (MQ=255)                                                                   gtgtCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGcc                                                                                >  4:326970/1‑149 (MQ=255)                                                                     gtCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGcc                                                                                >  2:235041/1‑147 (MQ=255)                                                                      tCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAg                                                                             >  5:107980/1‑149 (MQ=255)                                                                              ctCGATGTGTCCTCCGGTGGTACCTCGGTCTACGCCGATATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGGCACCGGCACCGTCGGCATGATCACTGAAGCTGCCCAGGCCGAGc                                                                      <  6:208081/147‑1 (MQ=255)                                                                              aTCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGca                                                                     >  5:231895/1‑149 (MQ=255)                                                                              aTCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGc                                                                      <  3:326970/148‑1 (MQ=255)                                                                               tCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGca                                                                     >  3:58243/1‑148 (MQ=255)                                                                                 gATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACAt                                                                  >  3:108301/1‑149 (MQ=255)                                                                                  aTGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAAGCCGAGCACAt                                                                  >  2:318157/1‑148 (MQ=255)                                                                                     tgtCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACt                                                                                         <  4:221113/122‑1 (MQ=255)                                                                                     tgtCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCt                                                               <  1:254492/148‑1 (MQ=255)                                                                                       tcctccGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGc                                                             <  1:239766/148‑1 (MQ=255)                                                                                       tcctccGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGc                                                             <  3:99911/148‑1 (MQ=255)                                                                                       tcctccGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGc                                                             <  5:88197/148‑1 (MQ=255)                                                                                       tcctccGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCg                                                            >  2:28077/1‑149 (MQ=255)                                                                                         ctccGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTa                                                          >  1:169272/1‑149 (MQ=255)                                                                                           ccGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGACATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTAcc                                                        >  6:201712/1‑149 (MQ=255)                                                                                                ggtACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGCAGCAGAGATCGCCACCGGCACCTTCGGCATGATCACTGAACCTTCCCAGGCATCGCAAATTCTGAGTACCggg                                                     >  4:202812/1‑147 (MQ=255)                                                                                                ggtACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTAGCAGACGCGCTTTGCAGCGCGCGTGCGCAAGGACGCAGAGATCGCCACCGGCACCGTCGGCATGAACCCTGCACCTGCCCCGGCCGCGCCCCTTCTGCGTACCggg                                                     >  4:207079/1‑147 (MQ=255)                                                                                                   aCCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGc                                                >  7:308637/1‑149 (MQ=255)                                                                                                    ccTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAgg                                                 <  8:206576/147‑1 (MQ=255)                                                                                                    ccTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAgg                                                 >  7:206576/1‑147 (MQ=255)                                                                                                    ccTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGAGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAggac                                               >  5:214283/1‑147 (MQ=255)                                                                                                        ggTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGAc                                            <  1:28077/148‑1 (MQ=255)                                                                                                        ggTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACg                                           <  1:246073/149‑1 (MQ=255)                                                                                                        ggTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACg                                           >  5:166708/1‑149 (MQ=255)                                                                                                        ggTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAAGCCGAGCACATTCTGCGTACCGGGCAGGCCGa                                             <  1:318157/147‑1 (MQ=255)                                                                                                        ggTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGACATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACg                                           <  5:201712/149‑1 (MQ=255)                                                                                                         gTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGt                                          <  6:231895/149‑1 (MQ=255)                                                                                                         gTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGt                                          >  1:320993/1‑149 (MQ=255)                                                                                                          tCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGt                                          >  4:13169/1‑148 (MQ=255)                                                                                                             aCGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATc                                      <  6:166708/149‑1 (MQ=255)                                                                                                                          tACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCgg                          >  1:159990/1‑148 (MQ=255)                                                                                                                           aCAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTcc                                  <  6:240128/139‑1 (MQ=255)                                                                                                                           aCAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTcc                                  >  5:240128/1‑139 (MQ=255)                                                                                                                            cAGGCCCTGGCTCTCCGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGCGCTCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCCGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCGGGa                        <  3:207079/148‑1 (MQ=255)                                                                                                                            cAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCGGGa                        <  4:108301/148‑1 (MQ=255)                                                                                                                            cAGGCCCTGGCTATCAGACGCGCTGTGCATAGCGCGTGCGCAATGAAGCAGAGATCGCCACAGGCACCGTCGGCATTATCACTGAACCTGCCCAGGCCGATCACATTCTGCGTACCGGGCAGTCCGACGTGATCTTCCTGGCTCGGGa                        <  3:202812/148‑1 (MQ=255)                                                                                                                               gCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCGGGAgctg                    <  8:308637/149‑1 (MQ=255)                                                                                                                                    ggCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCAGCCACGGCAGCAGCTgcg               >  4:1845/1‑149 (MQ=255)                                                                                                                                     gCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCGGGAGCtgctgc                <  6:107980/147‑1 (MQ=255)                                                                                                                                         tCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCGGGAGCTGCTGCGCGATc          <  2:320993/149‑1 (MQ=255)                                                                                                                                           gcaCGCGCTCTGCAAAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATc                                      <  4:35342/117‑1 (MQ=255)                                                                                                                                           gcaCGCGCTCTGCAAAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATc                                      >  3:35342/3‑119 (MQ=255)                                                                                                                                             aCGCGCTCTGCAAAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCGGGAGCTGCTGCGCGATCCTTa      >  5:137686/1‑149 (MQ=255)                                                                                                                                               gcgcTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCGGGAGCTGCTGCGCGATCCTTACt    >  3:119558/1‑149 (MQ=255)                                                                                                                                                 gcTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCGGGAGCTGCTGCGCGATCCTTACTgg  <  3:13169/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                  ACTGGGTCGAGGACGGCTGGAACCCGGATGAAACCGTCGAACTGGCTCGTCGCCTGCGGGTGCTGGGTGTCGACCTGATCGATGTGTCCTCCGGTGGTACCTCGGTCAACGCCGAAATTCCTACAGGCCCTGGCTATCAGACGCGCTTTGCAGAGCGCGTGCGCAAGGAAGCAGAGATCGCCACCGGCACCGTCGGCATGATCACTGAACCTGCCCAGGCCGAGCACATTCTGCGTACCGGGCAGGCCGACGTGATCTTCCTGGCTCGGGAGCTGCTGCGCGATCCTTACTGG  >  NC_002947/1684125‑1684417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑