Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,706,336	T→G	8.4%	M134R (ATG→AGG)	PP_1500 →	lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,706,336	0	T	G	8.4%	209.5 / 7.6	69	M134R (ATG→AGG)	PP_1500	lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (33/30);  new base G (3/3);  total (36/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCGGTAACCTGCCAGGGCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGATGGTGTG  >  NC_002947/1706191‑1706481                                                                                                                                                  |                                                                                                                                                   ggcggTAACCTGCCAGGGCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGc                                                                                                                                                 >  6:330115/1‑149 (MQ=255)          cTGCCAGGGCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTgca                                                                                                                                        <  5:230680/149‑1 (MQ=255)              cAGGGCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGcagcag                                                                                                                                    >  3:269010/1‑149 (MQ=255)                gggCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGgc                                                                                                                                  <  3:96801/149‑1 (MQ=255)                    aCCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGccc                                                                                                                              <  2:300738/149‑1 (MQ=255)                               aGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAggg                                                                                                                   >  3:90140/1‑149 (MQ=255)                                    cTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAgg                                                                                                                    >  3:200369/1‑143 (MQ=255)                                    cTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAgg                                                                                                                    <  4:200369/143‑1 (MQ=255)                                        cTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCt                                                                                                          >  8:124588/1‑149 (MQ=255)                                           ccGGAGCAGGGCTACCAGGTGCTGATGAGCGATTACCGCGGCTATGGCTTGGCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTgg                                                                                                        <  8:299631/148‑1 (MQ=255)                                             ggAGCATGGCTACCAGGGGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCCGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGTCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTAc                                                                                                     <  3:204135/149‑1 (MQ=255)                                             ggAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTAc                                                                                                     >  2:8355/1‑149 (MQ=255)                                                gCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTActgc                                                                                                  <  1:8355/149‑1 (MQ=255)                                                  aGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTActgctg                                                                                                <  1:180172/149‑1 (MQ=255)                                                   gggCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTgg                                                                                               <  4:269010/149‑1 (MQ=255)                                                   gggCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTgg                                                                                               <  5:330115/149‑1 (MQ=255)                                                    ggCTACCAGGTACTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAgg                                                                                                                                   <  3:65408/112‑1 (MQ=255)                                                    ggCTACCAGGTACTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAgg                                                                                                                                   >  4:65408/1‑112 (MQ=255)                                                     gCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTCCACCAGGCGCCCCAGGTCAAGGGCAAGCCGCTGGTACTGCTgggg                                                                                             >  5:199253/1‑149 (MQ=255)                                                      caacgaGGTGCTGTAGATCGATTACCGCGGGTATTGGTTGGTTCCGGGCCATCCGATCCTTGCGGAGGTTTACCAGGACATTGCTTCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCTAGCCGCTGGTACTGCTGGGGc                                                                                            <  6:199253/144‑1 (MQ=255)                                                      cTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCAGGCCATGGCTgcagcaat                                                                                                                                   >  3:328406/1‑108 (MQ=39)                                                      cTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCAGGCCATGGCTgcagcaat                                                                                                                                   <  4:328406/110‑3 (MQ=39)                                                         ccAGGTGCTGATTCTCGATTACCGCGGCTATGGCTGGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCAGGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCaga                                                                                         <  4:45534/149‑1 (MQ=255)                                                         ccAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCa                                                                                           >  6:338880/1‑147 (MQ=255)                                                                cTGAAGATCGATTCCCGCGGCTATGGCTTTTTTCACGGACAGCCGAGCCTGCCTGAGGTGTACCATGACATTGCTGCAGCCATTTCCTGTCTGCATCAGGCTCACGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGACAGAGCCTgg                                                                                   <  8:145470/148‑1 (MQ=255)                                                                    tgatCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCt                                                                                                                                               <  5:281180/84‑1 (MQ=255)                                                                    tgatCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCt                                                                                                                                               >  6:281180/1‑84 (MQ=255)                                                                     gatCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTg                                                                            >  3:250109/1‑149 (MQ=255)                                                                       tCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGc                                                                           >  7:146763/1‑148 (MQ=255)                                                                         gATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCAt                                                                        <  5:338880/149‑1 (MQ=255)                                                                              ccGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCca                                                                   >  8:176109/1‑149 (MQ=255)                                                                              ccGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCca                                                                   >  7:146922/1‑149 (MQ=255)                                                                               cgcgGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCcat                                                                  >  7:170171/1‑149 (MQ=255)                                                                                 cgGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCcattc                                                                <  7:176109/149‑1 (MQ=255)                                                                                      aTGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTAc                                                           >  7:41601/1‑149 (MQ=255)                                                                                       tGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTAc                                                           <  8:146763/148‑1 (MQ=255)                                                                                         gCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGCGCCTGGG‑CGGTGCCATGGCCATTCATTACCTg                                                        >  8:265956/1‑149 (MQ=255)                                                                                         gCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTg                                                        >  6:43259/1‑149 (MQ=255)                                                                                                  aGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCAc                                               >  4:243222/1‑149 (MQ=255)                                                                                                    ggCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCAccc                                             >  7:133161/1‑149 (MQ=255)                                                                                                    ggCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCAccc                                             >  6:270841/1‑149 (MQ=255)                                                                                                     gCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAGGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCGTGGCCATTCATTACCTGGCGGCCCGcccc                                            >  8:215691/1‑149 (MQ=255)                                                                                                      ccAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCAcccc                                            >  2:239951/1‑148 (MQ=255)                                                                                                      ccAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCg                                           <  5:43259/149‑1 (MQ=255)                                                                                                         gCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGag                                         >  8:259865/1‑148 (MQ=255)                                                                                                            gAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTATCTGGCGGCCCACCCCGagcagc                                     <  7:215691/149‑1 (MQ=255)                                                                                                             aGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGagcagc                                     <  1:239951/149‑1 (MQ=255)                                                                                                                cTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCa                                 <  7:124588/149‑1 (MQ=255)                                                                                                                  gCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAgc                               <  4:90140/149‑1 (MQ=255)                                                                                                                   ccGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAg                                <  5:270841/147‑1 (MQ=255)                                                                                                                    cGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCcattcatt                                                             <  8:321855/117‑1 (MQ=255)                                                                                                                    cGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCcattcatt                                                             >  7:321855/1‑117 (MQ=255)                                                                                                                     ggAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCt                            >  6:92655/1‑149 (MQ=255)                                                                                                                     ggAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCt                            >  7:67414/1‑149 (MQ=255)                                                                                                                       aGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTc                          <  4:250109/149‑1 (MQ=255)                                                                                                                          tgtACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAgc                       <  7:323889/149‑1 (MQ=255)                                                                                                                              ccAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGccc                   >  4:101201/1‑149 (MQ=255)                                                                                                                                aGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTg                 <  7:259865/149‑1 (MQ=255)                                                                                                                                  gACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGgt               <  5:92655/149‑1 (MQ=255)                                                                                                                                     aTTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGtt            >  3:238001/1‑149 (MQ=255)                                                                                                                                      ctgctgcAGCCAGGCCATGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTc           >  7:218472/2‑149 (MQ=255)                                                                                                                                         ctgcAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGAt        <  7:237889/149‑1 (MQ=255)                                                                                                                                          tgcAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGATg       >  2:33215/1‑149 (MQ=255)                                                                                                                                           gcAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGATgg      >  2:180980/1‑149 (MQ=255)                                                                                                                                           gcAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGATgg      >  1:174956/1‑149 (MQ=255)                                                                                                                                            cAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGATg       <  8:41601/147‑1 (MQ=255)                                                                                                                                            cAGCCAGGCCATGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTgg                                                                                               >  7:201952/1‑60 (MQ=37)                                                                                                                                            cAGCCAGGCCATGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTgg                                                                                               <  8:201952/60‑1 (MQ=37)                                                                                                                                               ccATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGATGGtgtg  >  5:332981/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                   GGCGGTAACCTGCCAGGGCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGATGGTGTG  >  NC_002947/1706191‑1706481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑