Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,706,340	C→A	6.8%	A135A (GCC→GCA) ‡	PP_1500 →	lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,706,340	0	C	A	6.8%	217.4 / 3.9	69	A135A (GCC→GCA) ‡	PP_1500	lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (32/32);  new base A (3/2);  total (35/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.93e-01

CTGCCAGGGCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGATGGTGTGCCGG  >  NC_002947/1706200‑1706485                                                                                                                                             |                                                                                                                                                   cTGCCAGGGCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTgca                                                                                                                                            <  5:230680/149‑1 (MQ=255)     cAGGGCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGcagcag                                                                                                                                        >  3:269010/1‑149 (MQ=255)       gggCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGgc                                                                                                                                      <  3:96801/149‑1 (MQ=255)           aCCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGccc                                                                                                                                  <  2:300738/149‑1 (MQ=255)                      aGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAggg                                                                                                                       >  3:90140/1‑149 (MQ=255)                           cTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAgg                                                                                                                        <  4:200369/143‑1 (MQ=255)                           cTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAgg                                                                                                                        >  3:200369/1‑143 (MQ=255)                               cTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCt                                                                                                              >  8:124588/1‑149 (MQ=255)                                  ccGGAGCAGGGCTACCAGGTGCTGATGAGCGATTACCGCGGCTATGGCTTGGCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTgg                                                                                                            <  8:299631/148‑1 (MQ=255)                                    ggAGCATGGCTACCAGGGGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCCGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGTCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTAc                                                                                                         <  3:204135/149‑1 (MQ=255)                                    ggAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTAc                                                                                                         >  2:8355/1‑149 (MQ=255)                                       gCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTActgc                                                                                                      <  1:8355/149‑1 (MQ=255)                                         aGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTActgctg                                                                                                    <  1:180172/149‑1 (MQ=255)                                          gggCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTgg                                                                                                   <  5:330115/149‑1 (MQ=255)                                          gggCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTgg                                                                                                   <  4:269010/149‑1 (MQ=255)                                           ggCTACCAGGTACTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAgg                                                                                                                                       <  3:65408/112‑1 (MQ=255)                                           ggCTACCAGGTACTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAgg                                                                                                                                       >  4:65408/1‑112 (MQ=255)                                            gCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTCCACCAGGCGCCCCAGGTCAAGGGCAAGCCGCTGGTACTGCTgggg                                                                                                 >  5:199253/1‑149 (MQ=255)                                             caacgaGGTGCTGTAGATCGATTACCGCGGGTATTGGTTGGTTCCGGGCCATCCGATCCTTGCGGAGGTTTACCAGGACATTGCTTCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCTAGCCGCTGGTACTGCTGGGGc                                                                                                <  6:199253/144‑1 (MQ=255)                                             cTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCAGGCCATGGCTgcagcaat                                                                                                                                       >  3:328406/1‑108 (MQ=39)                                             cTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCAGGCCATGGCTgcagcaat                                                                                                                                       <  4:328406/110‑3 (MQ=39)                                                ccAGGTGCTGATTCTCGATTACCGCGGCTATGGCTGGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCAGGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCaga                                                                                             <  4:45534/149‑1 (MQ=255)                                                ccAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCa                                                                                               >  6:338880/1‑147 (MQ=255)                                                       cTGAAGATCGATTCCCGCGGCTATGGCTTTTTTCACGGACAGCCGAGCCTGCCTGAGGTGTACCATGACATTGCTGCAGCCATTTCCTGTCTGCATCAGGCTCACGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGACAGAGCCTgg                                                                                       <  8:145470/148‑1 (MQ=255)                                                           tgatCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCt                                                                                                                                                   >  6:281180/1‑84 (MQ=255)                                                           tgatCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCt                                                                                                                                                   <  5:281180/84‑1 (MQ=255)                                                            gatCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTg                                                                                >  3:250109/1‑149 (MQ=255)                                                              tCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGc                                                                               >  7:146763/1‑148 (MQ=255)                                                                gATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCAt                                                                            <  5:338880/149‑1 (MQ=255)                                                                     ccGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCca                                                                       >  7:146922/1‑149 (MQ=255)                                                                     ccGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCca                                                                       >  8:176109/1‑149 (MQ=255)                                                                      cgcgGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCcat                                                                      >  7:170171/1‑149 (MQ=255)                                                                        cgGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCcattc                                                                    <  7:176109/149‑1 (MQ=255)                                                                             aTGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTAc                                                               >  7:41601/1‑149 (MQ=255)                                                                              tGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTAc                                                               <  8:146763/148‑1 (MQ=255)                                                                                gCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGCGCCTGGG‑CGGTGCCATGGCCATTCATTACCTg                                                            >  8:265956/1‑149 (MQ=255)                                                                                gCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTg                                                            >  6:43259/1‑149 (MQ=255)                                                                                         aGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCAc                                                   >  4:243222/1‑149 (MQ=255)                                                                                           ggCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCAccc                                                 >  6:270841/1‑149 (MQ=255)                                                                                           ggCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCAccc                                                 >  7:133161/1‑149 (MQ=255)                                                                                            gCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAGGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCGTGGCCATTCATTACCTGGCGGCCCGcccc                                                >  8:215691/1‑149 (MQ=255)                                                                                             ccAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCAcccc                                                >  2:239951/1‑148 (MQ=255)                                                                                             ccAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCg                                               <  5:43259/149‑1 (MQ=255)                                                                                                gCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGag                                             >  8:259865/1‑148 (MQ=255)                                                                                                   gAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTATCTGGCGGCCCACCCCGagcagc                                         <  7:215691/149‑1 (MQ=255)                                                                                                    aGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGGCCGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGagcagc                                         <  1:239951/149‑1 (MQ=255)                                                                                                       cTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCa                                     <  7:124588/149‑1 (MQ=255)                                                                                                         gCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAgc                                   <  4:90140/149‑1 (MQ=255)                                                                                                          ccGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAg                                    <  5:270841/147‑1 (MQ=255)                                                                                                           cGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCcattcatt                                                                 <  8:321855/117‑1 (MQ=255)                                                                                                           cGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCcattcatt                                                                 >  7:321855/1‑117 (MQ=255)                                                                                                            ggAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCt                                >  7:67414/1‑149 (MQ=255)                                                                                                            ggAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCt                                >  6:92655/1‑149 (MQ=255)                                                                                                              aGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTc                              <  4:250109/149‑1 (MQ=255)                                                                                                                 tgtACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAgc                           <  7:323889/149‑1 (MQ=255)                                                                                                                     ccAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGccc                       >  4:101201/1‑149 (MQ=255)                                                                                                                       aGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTg                     <  7:259865/149‑1 (MQ=255)                                                                                                                         gACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGgt                   <  5:92655/149‑1 (MQ=255)                                                                                                                            aTTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGtt                >  3:238001/1‑149 (MQ=255)                                                                                                                             ctgctgcAGCCAGGCCATGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTc               >  7:218472/2‑149 (MQ=255)                                                                                                                                ctgcAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGAt            <  7:237889/149‑1 (MQ=255)                                                                                                                                 tgcAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGATg           >  2:33215/1‑149 (MQ=255)                                                                                                                                  gcAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGATgg          >  2:180980/1‑149 (MQ=255)                                                                                                                                  gcAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGATgg          >  1:174956/1‑149 (MQ=255)                                                                                                                                   cAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGATg           <  8:41601/147‑1 (MQ=255)                                                                                                                                   cAGCCAGGCCATGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTgg                                                                                                   <  8:201952/60‑1 (MQ=37)                                                                                                                                   cAGCCAGGCCATGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTgg                                                                                                   >  7:201952/1‑60 (MQ=37)                                                                                                                                      ccATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGATGGtgtg      >  5:332981/1‑149 (MQ=255)                                                                                                                                           gCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGATGGTGTGCCgg  <  1:33215/148‑1 (MQ=255)                                                                                                                                             cTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGATGgtg        <  5:326687/140‑1 (MQ=255)                                                                                                                                             cTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGATGgtg        >  6:326687/1‑140 (MQ=255)                                                                                                                                             |                                                                                                                                                   CTGCCAGGGCACCTGGGGGGTAGCTACTGGCTGCCGGAGCAGGGCTACCAGGTGCTGATGATCGATTACCGCGGCTATGGCTTGTCTCAGGGCCAGCCGAGCCTGCCGGAGGTGTACCAGGACATTGCTGCAGCCATGGCCTGGCTGCAGCAGGCGCCCGAGGTCAAGGGCAAGCCGCTGGTACTGCTGGGGCAGAGCCTGGG‑CGGTGCCATGGCCATTCATTACCTGGCGGCCCACCCCGAGCAGCGTCAGCGCTTCAGCGCCCTGGTGTTCGATGGTGTGCCGG  >  NC_002947/1706200‑1706485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑