Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,744,260 G→C 9.4% intergenic (‑49/+18) PP_1541 ← / ← PP_1542 putative Methyltransferase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,744,2600GC 9.4% 213.1 / 10.4 72intergenic (‑49/+18)PP_1541/PP_1542putative Methyltransferase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (32/33);  new base C (5/2);  total (37/35)
Fisher's exact test for biased strand distribution p-value = 4.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.48e-01

GCGTTCGTCTACGTCGCCTGGCGCGATATGGCCCGCGGCAATGAGGTTGCGGAGCCACTGCGCGGCGTATGGGTCGATCTCGTTGTAATACGCAGCCATACGGCTCCTCGCCGGG‑GAGGCGTTATCGTTGAATAGGGGAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAGGCCGCGAGGCGCTTCTTGTCTGCTCGGCCCTTGGCCTGGTGCAGCCGAAGCGCTTCTTTGTAGCTGGCCTTGGCCGCTTTTATGGTCGGTGCCCACTCGCCGGCGACCTCAAGAGTCTCGTAGGGC  >  NC_002947/1744113‑1744407
                                                                                                                                                    |                                                                                                                                                   
gCGTTCGTCTACGTCGCCTGGCGCGATATGGCCCGCGGCAATGAGGTTGCGGAGCCACTGCGCGGCGTATGGGTCGATCTCGTTGTAATACGCAGCCATACGGCTCCTCGCCGGG‑GAGGCGTTATCGTTGAATAGGGGAAGGCGCTGGc                                                                                                                                                    >  5:350732/1‑149 (MQ=255)
      gTCTACGTCGCCTGGCGCGATATGGCCCGCGGCAATGAGGTTGCGGAGCCACTGCGCGGCGTATGGGTCGATCTCGTTGTAATACGCAGCCATACGGCTCCTCGCCGGG‑GAGGCGTTATCGTTGAATAGGGGAAGGCGCTGGCGGGCAg                                                                                                                                              >  1:299725/1‑149 (MQ=255)
      gTCTACGTCGCCTGGCGCGATATGGCCCGCGGCAATGAGGTTGCGGAGCCACTGCGCGGCGTATGGGTCGATCTCGTTGTAATACGCAGCCATACGGCTCCTCGCCGGG‑GAGGCGTTATCGTTGAATAGGGGAAGGCGCTGGCGGGCAg                                                                                                                                              >  4:286549/1‑149 (MQ=255)
           cGTCGCCTGGCGCGATATGGCCCGCGGCAATGAGGTTGCGGAGCCACTGCGCGGCGTATGGGTCGATCTCGTTGTAATACGCAGCCATACGGCTCCTCGCCGGG‑GAGGCGTTATCGTTGAATAGGGGAAGGCGCTGGCGGGCAGCGCgg                                                                                                                                         <  2:299725/149‑1 (MQ=255)
            gTCGCCTGGCGCGATATGGCCCGCGGCAATGAGGTTGCGGAGCCACTGCGCGGCGTATGGGTCGATCTCGTTGTAATACGCAGCCATACGGCTCCTCGCCGGG‑GAGGCGTTATCGTTGAATAGGGGAAGGCGCTGGCGGGCAGCGCggg                                                                                                                                        >  8:98980/1‑149 (MQ=255)
             tCGCCTGGCGCGATATGGCCCGCGGCAATGAGGTTGCGGAGCCACTGCGCGGCGTATGGGTCGATCTCGTTGTAATACGCAGCCATACGGCTCCTCGCCGGG‑GAGGCGTTATCGTTGAATAGGGGAAGGCGCTGGCGGGCAGCGCGGGa                                                                                                                                       <  2:338919/149‑1 (MQ=255)
             tCGCCTGGCGCGATATGGCCCGCGGCAATGAGGTTGCGGAGCCACTGCGCGGCGTATGGGTCGATCTCGTTGTAATACGCAGCCATACGGCTCCTCGCCGGG‑GAGGCGTTATCGTTGAATAGGGGAAGGCGCTGGCGGGCAGCGCGGGa                                                                                                                                       <  2:333721/149‑1 (MQ=255)
              cGCCTGGCGCGATATGGCCCGCGGCAATGAGGTTGCGGAGCCACTGCGCGGCGTATGGGTCGATCTCGTTGTAATACGCAGCCATACGGCTCCTCGCCGGG‑GAGGCGTTATCGTTGAATAGGGGAAGGCGCTGGCGGGCAGCGCGGGAg                                                                                                                                      <  5:117154/149‑1 (MQ=255)
               gCCTGGCGCGATATGGCCCGCGGCAATGAGGTTGCGGAGCCACTGCGCGGCGTATGGGTCGATCTCGTTGTAATACGCAGCCATACGGCTCCTCGCCGGG‑GAGGCGTTATCGTTGAATAGGGGAAGGCGCTGGCGGGCAGCGCGGGAgg                                                                                                                                     <  3:286549/149‑1 (MQ=255)
                     cgcgATATGGCCCGCGGCAATGAGGTTGCGGAGCCACTGCGCGGCGTATGGGTCGATCTCGTTGTAATACGCAGCCATACGGCTCCTCGCCGGG‑GAGGCGTTATCGTTGAATAGGGGAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAgg                                                                                                                               <  4:111843/149‑1 (MQ=255)
                        gATATGGCCCGCGGCAATGAGGTTGCGGAGCCACTGCGCGGCGTATGGGTCGATCTCGTTGTAATACGCAGCCATACGGCTCCTCGCCGGG‑GAGGCGTTATCGTTGAATAGGGGAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAGGCcg                                                                                                                            >  4:305912/1‑149 (MQ=255)
                          tatGGCCCGCGGCAATGAGGTTGCGGAGCCACTGCGCGGCGTATGGGTCGATCTCGTTGTAATACGCAGCCATACGGCTCCTCGCCGGG‑GAGGCGTTATCGTTGAATAGGGGAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAGGCcgc                                                                                                                           <  6:130378/148‑1 (MQ=255)
                              gCCCGCGGCAATGAGGTTGCGGAGCCACTGCGCGGCGTATGGGTCGATCTCGTTGTAATACGCAGCCATACGGCTCCTCGCCGGG‑GAGGCGTTATCGTTGAATAGGGGAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAGGCCGCGAgg                                                                                                                       <  3:305912/148‑1 (MQ=255)
                              gCCCGCGGCAATGAGGTTGCGGAGCCACTGCGCGGCGTATGGGTCGATCTCGTTGTAATACGCAGCCATACGGCTCCTCGCCGGG‑GAGGCGTTATCGTTGAATAGGGGAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAGGCCGCGAGgc                                                                                                                      >  3:175473/1‑149 (MQ=255)
                              gCCCGCGGCAATGAGGTTGCGGAGCCACTGCGCGGCGTATGGGTCGATCTCGTTGTAATACGCAGCCATACGGCTCCTCGCAGGG‑GAGGCGTTATCGTTGAATAGGGGAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAGGCCGCGAGgc                                                                                                                      >  7:283770/1‑149 (MQ=255)
                                ccGCGGCAATGAGGTTGCGGAGCCACTGCGCGGCGTATGGGTCGATCTCGTTGTAATACGCAGCCATACGGCTCCTCGCCGGG‑GAGGCGTTATCGTTGAATAGGGGAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAGGCCGCGAGgcgc                                                                                                                    <  5:42291/149‑1 (MQ=255)
                                ccGCGGCAATGAGGTTGCGGAGCCACTGCGCGGCGTATGGGTCGATCTCGTTGTAATACGCAGCCATACGGCTCCTCGCCGGG‑GAGGCGTTATCGTTGAATAGGGGAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAGGCCGCGAGgcgc                                                                                                                    >  7:6271/1‑149 (MQ=255)
                                    ggCAATGAGGTTGCGGAGCCACTGCGCGGCGTATGGGTCGATCTCGTTGTAATACGCAGCCATACGGCTCCTCGCCGGG‑GAGGCGTTATCGTTGAATAGGGGAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAGGCCGCGAGgcgc                                                                                                                    <  3:180538/145‑1 (MQ=255)
                                    ggCAATGAGGTTGCGGAGCCACTGCGCGGCGTATGGGTCGATCTCGTTGTAATACGCAGCCATACGGCTCCTCGCCGGG‑GAGGCGTTATCGTTGAATAGGGGAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAGGCCGCGAGgcgc                                                                                                                    >  4:180538/1‑145 (MQ=255)
                                      cAATGAGGTTGCGGAGCCACTGCGCGGCGTATGGGTCGATCTCGTTGTAATACGCAGCCATACGGCTCCTCGCCGGG‑GAGGCGTTATCGTTGAATAGGGGAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAGGCCGCGAGGCGCTTCTTg                                                                                                              <  7:273080/149‑1 (MQ=255)
                                       aaTGAGGTTGCGGAGCCACTGCGCGGCGTATGGGTCGATCTCGTTGTAATACGCAGCCATACGGCTCCTCGCCGGG‑GAGGCGTTATCGTTGAATAGGGGAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAGGCCGCGa                                                                                                                         <  3:111965/137‑1 (MQ=255)
                                       aaTGAGGTTGCGGAGCCACTGCGCGGCGTATGGGTCGATCTCGTTGTAATACGCAGCCATACGGCTCCTCGCCGGG‑GAGGCGTTATCGTTGAATAGGGGAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAGGCCGCGa                                                                                                                         >  4:111965/1‑137 (MQ=255)
                                        aTGAGGTTGCGGAGCCACTGCGCGGCGTATGGGTCGATCTCGTTGTAATACGCAGCCATACGGCTCCTCGCCGGG‑GAGGCGTTATCGTTGAATAGGGGAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAGGCCGCGAGGCGCTTCTTGTc                                                                                                            <  8:274198/149‑1 (MQ=255)
                                        aTGAGGTTGCGGAGCCACTGCGCGGCGTATGGGTCGATCTCGTTGTAATACGCAGCCATACGGCTCCTCGCCGGG‑GAGGCGTTATCGTTGAATAGGGGAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAGGCCGCGAGGCGCTTCTTGTc                                                                                                            <  4:175473/149‑1 (MQ=255)
                                             gTTGCGGAGCCACTGCGCGGCGTATGGGTCGATCTCGTTGTAATACGCAGCCATACGGCTCCTCGCCGGG‑GAGGCGTTATCGTTGAATAGGGGAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAGGCCGCGAGGCGCTTCTTGTCTGCTc                                                                                                       >  4:39539/1‑149 (MQ=255)
                                                     gCCACTGCGCGGCGTATGGGTCGATCTCGTTGTAATACGCAGCCATACGGCTCCTCGCCGTG‑GAGGCGTTATCGTTGAATAGGGGAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAGGCCGCGAGGCGCTTCTTGTCTGCTCGGCCCTTg                                                                                               <  6:350732/149‑1 (MQ=255)
                                                           gcgcGGCGTATGGGTCGATCTCGTTGTAATACGCAGACATACGGCTCATCGACCGG‑GAGGCGTTATCGTTGAATAGGGGAAGGCGCTGGCGGGCAGCGCGGGAGGCTTAGGACGCCAGGCGCTTCTTGTCTGCTCGGCCCTTGGCCTgg                                                                                         >  8:176903/1‑149 (MQ=37)
                                                                        gTCGATCTCGTTGTAATACGCAGCCATACGGCTCCTCGCCGGG‑GAGGCGTTATCGTTGAATAGGGGAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAGGCCGCGAGGCGCTTCTTGTCTGCTCGGCCCTTGGCCTGGTGCAGCCGAAgcg                                                                            >  7:150652/1‑149 (MQ=255)
                                                                            aTCTCGTTGTAATACGCAGCCATACGGCTCCTCGCCGGG‑GAGGCGTTATCGTTGAATAGGGGAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAGGCCGCGAGGCGCTTCTTGTCTGCTCGGCCCTTGGCCTGGTGCAGCCGAAGCGcttc                                                                        >  6:283204/1‑149 (MQ=255)
                                                                             tctcGTTGTAATACGCAGCCATACGGCTCCTCGCCGGG‑GAGGCGTTATCGTTGAATAGGGGAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAGGCCGCGAGGCGCTTCTTGTCTGCTCGGCCCTTGGCCTGGTGCAGCCGAAGCGcttct                                                                       >  7:46001/1‑149 (MQ=255)
                                                                               tcGTTGTAATACGCAGCCATACGGCTCCTCGCCGGG‑GAGGCGTTATCGTTGAATAGGGGAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAGGCCGCGAGGCGCTTCTTGTCTGCTCGGCCCTTGGCCTGGt                                                                                        <  6:86428/130‑1 (MQ=255)
                                                                               tcGTTGTAATACGCAGCCATACGGCTCCTCGCCGGG‑GAGGCGTTATCGTTGAATAGGGGAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAGGCCGCGAGGCGCTTCTTGTCTGCTCGGCCCTTGGCCTGGt                                                                                        >  5:86428/1‑130 (MQ=255)
                                                                                 gTTGTAATACGCAGCCATACGGCTCCTCGCCGGG‑GAGGCGTTATCGTTGAATAGGGGAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAGGCCGCGAGGCGCTTCTTGTCTGCTCGGCCCTTGGCCTGGTGCAGCCGAAGCGCTTCTTTg                                                                    >  2:63137/1‑148 (MQ=255)
                                                                                  ttGTAATACGCAGCCATACGGCTCCTCGCCGGGTGAGGCGTTATCGTTGAATAGGGGAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAGGCCGCGAGGCGCTTCTTGTCTGCTCGGCCCTTGGCCTGGTGCAGCCGAAGCGCTTCTTTg                                                                    >  1:46252/1‑148 (MQ=255)
                                                                                  ttGTAATACGCAGCCATACGGCTCCTCGCCGGG‑GAGGCGTTATCGTTGAATAGGGGAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAGGCCGCGAGGCGCTTCTTGTCTGCTCGGCCCTTGGCCTGGTGCAGCCGAAGCGCTTCTTTGTa                                                                  <  8:283770/149‑1 (MQ=255)
                                                                                    gTAATACGCAGCCATACGGCTCCTCGCCGGG‑GAGGCGTTATCGTTGAATAGGGGAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAGGCCGCGAGGCGCTTCTTGTCTGCTCGGCCCTTGGCCTGGTGCAGCCGAAGCGCTTCTTTGTa                                                                  <  8:41634/147‑1 (MQ=255)
                                                                                    gTAATACGCAGCCATACGGCTCCTCGCCGGG‑GAGGCGTTATCGTTGAATAGGGGAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAGGCCGCGAGGCGCTTCTTGTCTGCTCGGCCCTTGGCCTGGTGCAGCCGAAGCGCTTCTTTGTa                                                                  >  7:41634/1‑147 (MQ=255)
                                                                                    gTAATACGCAGCCATACGGCTCCTCGCCGGG‑GAGGCGTTATCGTTGAATAGGGGAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAGGCCGCGAGGCGCTTCTTGTCTGCTCGGCCCTTGGCCTGGTGCAGCCGAAGCGCTTCTTTGTAg                                                                 <  8:150652/148‑1 (MQ=255)
                                                                                      aaTACGCAGCCATACGGCTCCTCGCCGGG‑GAGGCGTTATCGTTGAATAGGGGAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAGGCCGCGAGGCGCTTCTTGTCTGCTCGGCCCTTGGCCTGGTGCAGCCGAAGCGCTTCTTTGTAGCTg                                                              >  4:8798/1‑149 (MQ=255)
                                                                                       aTACGCAGCCATACGGCTCCTCGCCGGG‑GAGGCGTTATCGTTGAATAGGGGAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAGGCCGCGAGGCGCTTCTTGTCTGCTCGGCCCTTGGCCTGGTGCAGCCGAAGCGCTTCTTTGTAGCTgg                                                             <  3:39539/149‑1 (MQ=255)
                                                                                         aCGCAGCCATACGGCTCCTCGCCGGG‑GAGGCGTTATCGTTGAATAGGGGAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAGGCCGCGAGGCGCTTCTTGTCTGCTCGGCCCTTGGCCTGGTGCAGCCGAAGCGCTTCTTTGTAGCTGGcc                                                           >  3:185154/1‑149 (MQ=255)
                                                                                           gCAGCCATACGGCTCCTCGCCGGG‑GAGGCGTTATCGTTGAATAGGGGAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAGGCCGCGAGGCGCTTCTTGTCTGCTCGGCCCTTGGCCTGGTGCAGCCGAAGCGCTTCTTTGTAGCTGGCCtt                                                         >  5:49339/1‑149 (MQ=255)
                                                                                           gCAGCCATACGGCTCCTCGCCGGG‑GAGGCGTTATCGTTGAATAGGGGAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAGGCCGCGAGGCGCTTCTTGTCTGCTCGGCCCTTGGCCTGGTGCAGCCGAAGCGCTTCTTTGTAGCTGGCCtt                                                         >  8:67970/1‑149 (MQ=255)
                                                                                             aGCCATACGGCTCCTCGCCGGG‑GAGGCGTTATCGTTGAATAGGGGAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAGGCCGCGAGGCGCTTCTTGTCTGCTCGGCCCTTGGCCTGGTGCAGCCGAAGCGCTTCTTTGTAGCTGGCCTTgg                                                       <  8:6271/149‑1 (MQ=255)
                                                                                                        tcctcGCCGGG‑GAGGCGTTATCGTTGAATAGGGGAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAGGCCGCGAGGCGCTTCTTGTCTGCTCGGCCCTTGGCCTGGTGCAGCCGAAGCGCTTCTTTGTAGCTGGCCTTGGCCGCTTTTATg                                            >  1:288832/1‑149 (MQ=255)
                                                                                                        tcctcGCCGGG‑GAGGCGTTATCGTTGAATAGGGGAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAGGCCGCGAGGCGCTTCTTGTCTGCTCGGCCCTTGGCCTGGTGCAGCCGAAGCGCTTCTTTGTAGCTGGCCTTGGCCGCTTTTATg                                            >  1:237939/1‑149 (MQ=255)
                                                                                                          ctcGCCGGG‑GAGGCGTTATCGTTGAATAGGGGAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAGGCCGCGAGGCGCTTCTTGTCTGCTCGGCCCTTGGCCTGGTGCAGCCGAAGCGCTTCTTTGTAGCTGGCCTTGGCCGCTTTTATg                                            <  6:329399/147‑1 (MQ=255)
                                                                                                          ctcGCCGGG‑GAGGCGTTATCGTTGAATAGGGGAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAGGCCGCGAGGCGCTTCTTGTCTGCTCGGCCCTTGGCCTGGTGCAGCCGAAGCGCTTCTTTGTAGCTGGCCTTGGCCGCTTTTATGGt                                          <  2:237939/149‑1 (MQ=255)
                                                                                                          ctcGCCGGG‑GAGGCGTTATCGTTGAATAGGGGAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAGGCCGCGAGGCGCTTCTTGTCTGCTCGGCCCTTGGCCTGGTGCAGCCGAAGCGCTTCTTTGTAGCTGGCCTTGGCCGCTTTTATGGt                                          >  5:102017/1‑149 (MQ=255)
                                                                                                           tcGCCGGG‑GAGGCGTTATCGTTGAATAGGGGAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAGGCCGCGAGGCGCTTCTTGTCTGCTCGGCCCTTGGCCTGGTGCAGCCGAAGCGCTTCTTTGTAGCTGGCCTTGGCCGCTTTTATGGTc                                         >  8:165855/1‑149 (MQ=255)
                                                                                                              ccGGG‑GAGGCGTTATCGTTGATGAGGGGAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAGGCCGCGAGGCGCTTCTTGTCTGCTCGGCCCGTGGCCTGGTGCAGCCGAAGCGCTTCGTTGTAGCTGGCGGTGGCCGCATTTATGGTCGGt                                      >  8:224531/1‑149 (MQ=37)
                                                                                                                ggg‑gATGCGTTATCGTTCAATAGGTGAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAGGCCGCGAGGCGCTTCTTGTCTGCTCGGCCCTTGGCCTGGTTCAGCCGAAGCGCTTCTTTGTAGCTGGCCTTGGCCGCTTTTATTGTCGGTGc                                    <  7:176903/149‑1 (MQ=38)
                                                                                                                    gAGGCGTTATCGTTGAATAGGGGAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAGGCCGCGAGGCGCTTCTTGTCTGCTCGGCCCTTGGCCTGGTGCAGCCGAAGCGCTTCTTTGTAGCTGGCCTTGGCCGCTTTTATGGTCGGTGCCCa                                 <  1:63137/149‑1 (MQ=255)
                                                                                                                                  gAATAGGGGAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAGGCCGCGAGGCGCTTCTTGTCTGCTCGGCCCTTGGCCTGGTGCAGCCGAAGCGCTTCTTTGTAGCTGGCCTTGGCCGCTTTTATGGTCGGTGCCCACTCGCCGGCGAcc                    <  2:288832/148‑1 (MQ=255)
                                                                                                                                   aaTAGGGGAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAGGCCGCTAGGCGCTTCTTGTCTGCTCGGCCCTTGGCCTGGTGCAGCCGAAGCGCTTCTTTGTAGCTGGCCTTGGCCGCTTTTATGGTCGGTGCCCACTCGCCGGCGACCt                   <  7:98980/148‑1 (MQ=255)
                                                                                                                                   aaTAGGGGAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAGGCCGCGAGGCGCTTCTTGTCTGCTCGGCCCTTGGCCTGGTGCAGCCGAAGCGCTTCTTTGTAGCTGGCCTTGGCCGCTTTTATGGTCg                                        <  6:302792/127‑1 (MQ=255)
                                                                                                                                   aaTAGGGGAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAGGCCGCGAGGCGCTTCTTGTCTGCTCGGCCCTTGGCCTGGTGCAGCCGAAGCGCTTCTTTGTAGCTGGCCTTGGCCGCTTTTATGGTCGGTGCCCACTCGCCGGCGACCt                   >  3:294042/1‑148 (MQ=255)
                                                                                                                                   aaTAGGGGAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAGGCCGCGAGGCGCTTCTTGTCTGCTCGGCCCTTGGCCTGGTGCAGCCGAAGCGCTTCTTTGTAGCTGGCCTTGGCCGCTTTTATGGTCGGTGCCCACTCGCCGGCGACCt                   >  1:30065/1‑148 (MQ=255)
                                                                                                                                   aaTAGGGGAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAGGCCGCGAGGCGCTTCTTGTCTGCTCGGCCCTTGGCCTGGTGCAGCCGAAGCGCTTCTTTGTAGCTGGCCTTGGCCGCTTTTATGGTCGGTGCCCACTCGCCGGCGACCTc                  <  4:185154/149‑1 (MQ=255)
                                                                                                                                     tAGGGGAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAGGCCGCGAGGCGCTTCTTGTCTGCTCGGCCCTTGGCCTGGTGCAGCCGAAGCGCTTCTTTGTAGCTGGCCTTGGCCGCTTTTATGGTCGGTGCCCACTCGCCGGCGACCTCaa                <  7:165855/149‑1 (MQ=255)
                                                                                                                                     tAGGGGAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAGGCCGCGAGGCGCTTCTTGTCTGCTCGGCCCTTGGCCTGGTGCAGCCGAAGCGCTTCTTTGTAGCTGGCCTTGGCCGCTTTTATGGTCGGTGCCCACTCGCCGGCGACCTCaa                <  4:294042/149‑1 (MQ=255)
                                                                                                                                        gggAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAGGCCGCGAGGCGCTTCTTGTCTGCTCGGCCCTTGGCCTGGTGCAGCCGAAGCGCTTCTTTGTAGCTGGCCTTGGCCGCTTTTATGGTCGGTGCCCACTCGCCGGCGACCTCAagag             >  2:124179/1‑149 (MQ=255)
                                                                                                                                         ctcccgcgcTGCCCGCCAGCGCGGGAGGGTTAGGCCGCGAGGCGCTTCTTGTCTGCTCGGCCCTTGGCCTGGTGCAGCCGAAGCGCTTCTTTGTAGCTGGCCTTGGCCGCTTTTATGGTCGGTGc                                    >  1:304191/6‑125 (MQ=255)
                                                                                                                                         ctcccgcgcTGCCCGCCAGCGCGGGAGGGTTAGGCCGCGAGGCGCTTCTTGTCTGCTCGGCCCTTGGCCTGGTGCAGCCGAAGCGCTTCTTTGTAGCTGGCCTTGGCCGCTTTTATGGTCGGTGc                                    <  2:304191/120‑1 (MQ=255)
                                                                                                                                          tcccgcgcTGCCCGCCAGCGCGGGAGGGTTAGGCCGCGAGGCGCTTCTTGTCTGCTCGGCCCTTGGCCTGGTGCAGCCGAAGCGCTTCTTTGTAGCTGGCCTTGGCCGCTTTTATGGTCGGTGCCCACTCGCCGGCGACCTCAAGAGtc           >  5:58412/5‑149 (MQ=255)
                                                                                                                                          tcccgcgcTGCCCGCCAGCGCGGGAGGGTTAGGCCGCGAGGCGCTTCTTGTCTGCTCGGCCCTTGGCCTGGTGCAGCCGAAGCGCTTCTTTGTAGCTGGCCTTGGCCGCTTTTATGGTCGGTGCCCACTCGCCGGCGACCTCAAGAGt            >  8:26118/5‑148 (MQ=255)
                                                                                                                                            ccgcgcTGCCCGCCAGCGCGGGAGGGTTAGGCCGCGAGGCGCTTCTTGTCTGCTCGGCCCTTGGCCTGGTGCAGCCGAAGCGCTTCTTTGTAGCTGGCCTTGGCCGCTTTTATGGTCGGTGCCCACTCGCCGGCGACCTCAAGAGtctc         >  8:93491/3‑149 (MQ=255)
                                                                                                                                            ccgcgcTGCCCGCCAGCGCGGGAGGGTTAGGCCGCGAGGCGCTTCTTGTCTGCTCGGCCCTTGGCCTGGTGCAGCCGAAGCGCTTCTTTGTAGCTGGCCTTGGCCGCTTTTATGGTCGGTGCCCACTCGCCGGCGACCTCAAGAGt            <  7:26118/144‑1 (MQ=255)
                                                                                                                                             ggCGCTGGCGGGCAGCGCGGGAGGGTTAGGCCGCGAGGCGCTTCTTGTCTGCTCGGCCCTTGGCCTGGTGCAGCCGAAGCGCTTCTTTGTAGCTGGCCTTGGCCGCTTTTATGGTCGGTGCCCACTCGCCgg                         <  6:98451/132‑1 (MQ=255)
                                                                                                                                             ggCGCTGGCGGGCAGCGCGGGAGGGTTAGGCCGCGAGGCGCTTCTTGTCTGCTCGGCCCTTGGCCTGGTGCAGCCGAAGCGCTTCTTTGTAGCTGGCCTTGGCCGCTTTTATGGTCGGTGCCCACTCGCCgg                         >  5:98451/1‑132 (MQ=255)
                                                                                                                                             cgcgcTGCCCGCCAGCGCGGGAGGGTTAGGCCGCGAGGCGCTTCTTGTCTGCTCGGCCCTTGGCCTGGTGCAGCCGAAGCGCTTCTTTGTAGCTGGCCTTGGCCGCTTTTATGGTCGGTGCCCACTCGCCGGCGACCTCAAGAGTCTCg        >  7:201754/2‑149 (MQ=255)
                                                                                                                                                 cTGGCGGGCAGCGCGGGAGGGTTAGGCCGCGAGGCGCTTCTTGTCTGCTCGGCCCTTGGCCTGGTGCAGCCGAAGCGCTTCTTTGTAGCTGGCCTTGGCCGCTTTTATGGTCGGTGCCCACTCGCCGGCGACCTCAAGAGTCTCGTAgg    <  2:46252/149‑1 (MQ=255)
                                                                                                                                                   ggCGGGCAGCGCGGGAGGGTTAGGCCGCGAGGCGCTTCTTGTCTGCTCGGCCCTTGGCCTGGTGCAGCCGAAGCGCTTCTTTGTAGCTGGCCTTGGCCGCTTTTATGGTCGGTGCCCACTCGCCGGCGACCTCAAGAGTCTCGTAGGGc  <  6:49339/149‑1 (MQ=255)
                                                                                                                                                    gCGGGCAGCGCGGGAGGGTTAGGCCGCGAGGCGCTTCTTGTCTGCTCGGCCCTTGGCCTGGTGCAGCCGAAGCGCTTCTTTGTAGCTGGCCTTGGCCGCTTTTATGGTCGGTGCCCACTCGCCGGCGACCTCAAGAGTCTCGTAGGGc  <  7:67970/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCGTTCGTCTACGTCGCCTGGCGCGATATGGCCCGCGGCAATGAGGTTGCGGAGCCACTGCGCGGCGTATGGGTCGATCTCGTTGTAATACGCAGCCATACGGCTCCTCGCCGGG‑GAGGCGTTATCGTTGAATAGGGGAAGGCGCTGGCGGGCAGCGCGGGAGGGTTAGGCCGCGAGGCGCTTCTTGTCTGCTCGGCCCTTGGCCTGGTGCAGCCGAAGCGCTTCTTTGTAGCTGGCCTTGGCCGCTTTTATGGTCGGTGCCCACTCGCCGGCGACCTCAAGAGTCTCGTAGGGC  >  NC_002947/1744113‑1744407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑