Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,751,792	C→G	7.0%	R67G (CGC→GGC)	PP_1552 →	replication protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,751,792	0	C	G	7.0%	223.2 / 4.7	69	R67G (CGC→GGC)	PP_1552	replication protein
Reads supporting (aligned to +/- strand):  ref base C (35/29);  new base G (3/2);  total (38/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01

GCCATTGCCGAGCGCCAGGCCAAGCAACAGGCCGAGGAAACCCGCCTGAAGCGCGAGGCCATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAGACCCACCTGGGCGCCGGTATGGCCAACGAGCTGATGCGCAACACCT  >  NC_002947/1751645‑1751939                                                                                                                                                    |                                                                                                                                                    gccATTGCCGAGCGCCAGGCCAAGCAACAGGCCGAGGAAACCCGCCTGAAGCGCGAGGCCATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCAAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAgcg                                                                                                                                                    <  2:56210/149‑1 (MQ=255)        ccGAGCGCCAGGCCAAGCAACAGGCCGAGGAAACCCGCCTGAAGCGCGAGGCCATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGcc                                                                                                                                             <  2:156012/149‑1 (MQ=255)               ccAGGCCAAGCAACAGGCCGAGGAAACCCGCCTGAAGCGCGAGGCCATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCt                                                                                                                                      <  7:268930/149‑1 (MQ=255)                cAGGCCAAGCAACAGGCCGAGGAAACCCGCCTGAAGCGCGAGGCCATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTa                                                                                                                                     >  4:235308/1‑149 (MQ=255)                  ggCCAAGCAACAGGCCGAGGAAACCCGCCTGAAGCGCGAGGCCATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGGGCCTTGCGCTGGCCttcg                                                                                                                                   <  4:49152/149‑4 (MQ=255)                   gCCAAGCAACAGGCCGAGGAAACCCGCCTGAAGCGCGAGGCCATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCt                                                                                                                                           <  8:247224/140‑1 (MQ=255)                   gCCAAGCAACAGGCCGAGGAAACCCGCCTGAAGCGCGAGGCCATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCt                                                                                                                                           >  7:247224/1‑140 (MQ=255)                           aCAGGCCGAGGAAACCCGCCTGAAGCGCGAGGCCATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGtt                                                                                                                          <  4:155540/149‑1 (MQ=255)                           aCAGGCCGAGGAAACCCGCCTGAAGCGCGAGGCCATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGt                                                                                                                           >  6:149537/1‑148 (MQ=255)                           aCAGGCCGAGGAAACCCGCCTGAAGCGCGAGGCCATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGt                                                                                                                           >  4:54984/1‑148 (MQ=255)                            cAGGCCGAGGAAACCCGCCTGAAGCGCGAGGCCATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAgc                                                                                                                                                     <  7:80533/121‑1 (MQ=255)                            cAGGCCGAGGAAACCCGCCTGAAGCGCGAGGCCATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAgc                                                                                                                                                     >  8:80533/1‑121 (MQ=255)                               gCCGAGGAAACCCGCCTGAAGCGCGAGGCCATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTa                                                                                                                                     <  5:284143/134‑1 (MQ=255)                               gCCGAGGAAACCCGCCTGAAGCGCGAGGCCATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTa                                                                                                                                     >  6:284143/1‑134 (MQ=255)                                    ggAAACCCGCCTGAAGCGCGAGGCCATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCcg                                                                                                                 <  4:229320/149‑1 (MQ=255)                                    ggAAACCCGCCTGAAGCGCGAGGCCATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCcg                                                                                                                 >  2:338936/1‑149 (MQ=255)                                         cccGCCTGAAGCGCGAGGCCATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTc                                                                                                            <  1:338936/149‑1 (MQ=255)                                          ccGCCTGAAGCGCGAGGCCATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGGGCCTTGCGCTGGCCt                                                                                                                                      <  1:168518/122‑1 (MQ=255)                                          ccGCCTGAAGCGCGAGGCCATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGGGCCTTGCGCTGGCCt                                                                                                                                      >  2:168518/1‑122 (MQ=255)                                          ccGCCTGAAGCGCGAGGCCATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTc                                                                                                            >  5:186639/1‑148 (MQ=255)                                          ccGCCTGAAGCGCGAGGCCATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTc                                                                                                            >  7:41151/1‑148 (MQ=255)                                           cGCCTGAAGCGCGAGGCCATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCga                                                                                                          <  7:117399/149‑1 (MQ=255)                                               tGAAGCGCGAGGCCATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCgag                                                                                                         >  7:213818/1‑146 (MQ=255)                                               tGAAGCGCGAGGCCATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCgag                                                                                                         >  2:57091/1‑146 (MQ=255)                                                  aGCGCGAGGCCATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATc                                                                                                   <  3:235308/149‑1 (MQ=255)                                                      cgAGGCCATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAgc                                                                                               <  8:41151/149‑1 (MQ=255)                                                            cATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCg                                                                                         >  7:17758/1‑149 (MQ=255)                                                             aTGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCgg                                                                                        >  4:279638/1‑149 (MQ=255)                                                             aTGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCgg                                                                                        <  3:54984/149‑1 (MQ=255)                                                             aTGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCgg                                                                                        >  3:205063/1‑149 (MQ=255)                                                               gACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGc                                                                                      >  7:69241/1‑149 (MQ=255)                                                                aCCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGc                                                                                      <  5:149537/148‑1 (MQ=255)                                                                 cccGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCa                                                                                            >  1:12963/1‑141 (MQ=255)                                                                 cccGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCa                                                                                            <  2:12963/141‑1 (MQ=255)                                                                 cccGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGt                                                                                    >  1:183988/1‑149 (MQ=255)                                                                  ccGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGtt                                                                                   >  2:292831/1‑149 (MQ=255)                                                                    gCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGc                                                                                 <  2:183988/149‑1 (MQ=255)                                                                       aGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTg                                                                              <  3:294263/149‑1 (MQ=255)                                                                                       gATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAggg                                                              >  7:209699/1‑149 (MQ=255)                                                                                         tACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTc                                                            <  8:17758/149‑1 (MQ=255)                                                                                          aCCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGAtgct                                                                         <  6:49870/135‑1 (MQ=255)                                                                                          aCCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCg                                                           >  6:253543/1‑149 (MQ=255)                                                                                           ccGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCgg                                                          <  6:186639/149‑1 (MQ=255)                                                                                            cGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGc                                                         >  7:215355/1‑149 (MQ=255)                                                                                                   tttCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGc                                                   >  3:206935/1‑148 (MQ=255)                                                                                                     tCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGc                                                   >  3:315473/1‑146 (MQ=255)                                                                                                      cGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAg                                                <  8:215355/148‑1 (MQ=255)                                                                                                       gCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAGAc                                              >  7:88699/1‑149 (MQ=255)                                                                                                         cGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAGAcc                                             >  4:240187/1‑148 (MQ=255)                                                                                                          gAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAGACCCa                                           >  4:99362/1‑149 (MQ=255)                                                                                                                    tGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAGACCCACCTGGGCGcc                                 >  7:254299/1‑149 (MQ=255)                                                                                                                    tGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAGACCCACCTGGGCGcc                                 >  7:172060/1‑149 (MQ=255)                                                                                                                       ccAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAGACCCACCTGGGCGCCGGt                              <  4:205063/149‑1 (MQ=255)                                                                                                                        cAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAGACCCACCTGGGCGCCGGTa                             <  7:42509/149‑1 (MQ=255)                                                                                                                         aaCTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAGACCCACCTGGGCGCCGGTAt                            <  3:226439/149‑1 (MQ=255)                                                                                                                            tACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGc                                                                                 >  4:136784/1‑93 (MQ=255)                                                                                                                            tACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGc                                                                                 <  3:136784/93‑1 (MQ=255)                                                                                                                              ccGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAGACCCACCTGGGCGCCGGTATGGcc                        >  5:181270/1‑148 (MQ=255)                                                                                                                              ccGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAGACCCACCTGGGCGCCGGTATGGc                         <  4:315473/147‑1 (MQ=255)                                                                                                                               cGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAGACCCACCTGGGCGCCGGTATGGCCaa                      >  5:32301/1‑149 (MQ=255)                                                                                                                                     gAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAGACCCACCTGGGCGCCGGTATGGCCAACGAGc                 <  3:129681/148‑1 (MQ=255)                                                                                                                                     gAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAGACCCACCTGGGCGCCGGTATGGCCAACGAGc                 >  4:129681/1‑148 (MQ=255)                                                                                                                                     gAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAGACCCACCTGGGCGCCGGTATGGCCAACGAGCt                <  3:279638/149‑1 (MQ=255)                                                                                                                                      aGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAGACCCACCTGGGCGCCGGTATGGCCAACGAGCt                >  1:195198/1‑148 (MQ=255)                                                                                                                                        cACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAGACCCACCTGGGCGCCGGTATGGCCAACGAGCTGAt             <  6:32301/149‑1 (MQ=255)                                                                                                                                            taGGCCAGGGCCTTGCGCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAGACCCACCTGGGCGCCGGTATGGCCAACGAGCTGATgcg          >  2:299157/2‑148 (MQ=255)                                                                                                                                             aGGCCAGGGCCTTGCGCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAGACCCACCTGGGCGCCGGTATGGCCAACGAGCTGATgcgc         >  1:38645/1‑148 (MQ=255)                                                                                                                                             aGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGATATCGAGCGCACCGGTCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAGACCCACCTGGGCGCCGGTATGGCCAACGAGCTGATgcgc         <  8:88699/148‑1 (MQ=255)                                                                                                                                              ggCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAGACCCACCTGGGCGCCGGTATGGCCAACGAGCTGATGCGCaa       <  1:57091/149‑1 (MQ=255)                                                                                                                                               gCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAGACCCACCTGGGCGCCGGTATGGCCAACGAGCTGATGCGCaa       >  1:335176/1‑148 (MQ=255)                                                                                                                                                 cAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAGACCCACCTGGGCGCCGGTATGGCCAACGAGCTGATGCGCAacac    >  4:283320/1‑149 (MQ=255)                                                                                                                                                  aGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAGACCCACCTGGGCGCCGGTATGGCCAACGAGCTGATGCGCAACAcc   >  4:108631/1‑149 (MQ=255)                                                                                                                                                   gcgcAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAGACCCACCTGGGCGCCGGTATGGCCAACGAGCTGATGCGCAACACCt  <  5:253543/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    GCCATTGCCGAGCGCCAGGCCAAGCAACAGGCCGAGGAAACCCGCCTGAAGCGCGAGGCCATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAGACCCACCTGGGCGCCGGTATGGCCAACGAGCTGATGCGCAACACCT  >  NC_002947/1751645‑1751939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑