Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,751,796	A→T	7.1%	K68M (AAG→ATG) ‡	PP_1552 →	replication protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,751,796	0	A	T	7.1%	210.5 / 4.7	70	K68M (AAG→ATG) ‡	PP_1552	replication protein
Reads supporting (aligned to +/- strand):  ref base A (35/30);  new base T (3/2);  total (38/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.29e-01

CCGAGCGCCAGGCCAAGCAACAGGCCGAGGAAACCCGCCTGAAGCGCGAGGCCATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAGACCCACCTGGGCGCCGGTATGGCCAACGAGCTGATGCGCAACACCTCGGC  >  NC_002947/1751652‑1751943                                                                                                                                                 |                                                                                                                                                    ccGAGCGCCAGGCCAAGCAACAGGCCGAGGAAACCCGCCTGAAGCGCGAGGCCATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGcc                                                                                                                                                 <  2:156012/149‑1 (MQ=255)        ccAGGCCAAGCAACAGGCCGAGGAAACCCGCCTGAAGCGCGAGGCCATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCt                                                                                                                                          <  7:268930/149‑1 (MQ=255)         cAGGCCAAGCAACAGGCCGAGGAAACCCGCCTGAAGCGCGAGGCCATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTa                                                                                                                                         >  4:235308/1‑149 (MQ=255)           ggCCAAGCAACAGGCCGAGGAAACCCGCCTGAAGCGCGAGGCCATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGGGCCTTGCGCTGGCCttcg                                                                                                                                       <  4:49152/149‑4 (MQ=255)            gCCAAGCAACAGGCCGAGGAAACCCGCCTGAAGCGCGAGGCCATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCt                                                                                                                                               >  7:247224/1‑140 (MQ=255)            gCCAAGCAACAGGCCGAGGAAACCCGCCTGAAGCGCGAGGCCATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCt                                                                                                                                               <  8:247224/140‑1 (MQ=255)                    aCAGGCCGAGGAAACCCGCCTGAAGCGCGAGGCCATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGtt                                                                                                                              <  4:155540/149‑1 (MQ=255)                    aCAGGCCGAGGAAACCCGCCTGAAGCGCGAGGCCATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGt                                                                                                                               >  6:149537/1‑148 (MQ=255)                    aCAGGCCGAGGAAACCCGCCTGAAGCGCGAGGCCATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGt                                                                                                                               >  4:54984/1‑148 (MQ=255)                        gCCGAGGAAACCCGCCTGAAGCGCGAGGCCATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTa                                                                                                                                         >  6:284143/1‑134 (MQ=255)                        gCCGAGGAAACCCGCCTGAAGCGCGAGGCCATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTa                                                                                                                                         <  5:284143/134‑1 (MQ=255)                             ggAAACCCGCCTGAAGCGCGAGGCCATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCcg                                                                                                                     <  4:229320/149‑1 (MQ=255)                             ggAAACCCGCCTGAAGCGCGAGGCCATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCcg                                                                                                                     >  2:338936/1‑149 (MQ=255)                                  cccGCCTGAAGCGCGAGGCCATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTc                                                                                                                <  1:338936/149‑1 (MQ=255)                                   ccGCCTGAAGCGCGAGGCCATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGGGCCTTGCGCTGGCCt                                                                                                                                          <  1:168518/122‑1 (MQ=255)                                   ccGCCTGAAGCGCGAGGCCATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGGGCCTTGCGCTGGCCt                                                                                                                                          >  2:168518/1‑122 (MQ=255)                                   ccGCCTGAAGCGCGAGGCCATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTc                                                                                                                >  5:186639/1‑148 (MQ=255)                                   ccGCCTGAAGCGCGAGGCCATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTc                                                                                                                >  7:41151/1‑148 (MQ=255)                                    cGCCTGAAGCGCGAGGCCATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCga                                                                                                              <  7:117399/149‑1 (MQ=255)                                        tGAAGCGCGAGGCCATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCgag                                                                                                             >  2:57091/1‑146 (MQ=255)                                        tGAAGCGCGAGGCCATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCgag                                                                                                             >  7:213818/1‑146 (MQ=255)                                           aGCGCGAGGCCATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATc                                                                                                       <  3:235308/149‑1 (MQ=255)                                               cgAGGCCATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAgc                                                                                                   <  8:41151/149‑1 (MQ=255)                                                     cATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCg                                                                                             >  7:17758/1‑149 (MQ=255)                                                      aTGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCgg                                                                                            <  3:54984/149‑1 (MQ=255)                                                      aTGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCgg                                                                                            >  3:205063/1‑149 (MQ=255)                                                      aTGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCgg                                                                                            >  4:279638/1‑149 (MQ=255)                                                        gACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGc                                                                                          >  7:69241/1‑149 (MQ=255)                                                         aCCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGc                                                                                          <  5:149537/148‑1 (MQ=255)                                                          cccGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCa                                                                                                >  1:12963/1‑141 (MQ=255)                                                          cccGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCa                                                                                                <  2:12963/141‑1 (MQ=255)                                                          cccGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGt                                                                                        >  1:183988/1‑149 (MQ=255)                                                           ccGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGtt                                                                                       >  2:292831/1‑149 (MQ=255)                                                             gCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGc                                                                                     <  2:183988/149‑1 (MQ=255)                                                                aGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTg                                                                                  <  3:294263/149‑1 (MQ=255)                                                                                gATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAggg                                                                  >  7:209699/1‑149 (MQ=255)                                                                                  tACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTc                                                                <  8:17758/149‑1 (MQ=255)                                                                                   aCCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGAtgct                                                                             <  6:49870/135‑1 (MQ=255)                                                                                   aCCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCg                                                               >  6:253543/1‑149 (MQ=255)                                                                                    ccGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCgg                                                              <  6:186639/149‑1 (MQ=255)                                                                                     cGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGc                                                             >  7:215355/1‑149 (MQ=255)                                                                                            tttCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGc                                                       >  3:206935/1‑148 (MQ=255)                                                                                              tCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGc                                                       >  3:315473/1‑146 (MQ=255)                                                                                               cGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAg                                                    <  8:215355/148‑1 (MQ=255)                                                                                                gCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAGAc                                                  >  7:88699/1‑149 (MQ=255)                                                                                                  cGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAGAcc                                                 >  4:240187/1‑148 (MQ=255)                                                                                                   gAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAGACCCa                                               >  4:99362/1‑149 (MQ=255)                                                                                                             tGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAGACCCACCTGGGCGcc                                     >  7:172060/1‑149 (MQ=255)                                                                                                             tGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAGACCCACCTGGGCGcc                                     >  7:254299/1‑149 (MQ=255)                                                                                                                ccAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAGACCCACCTGGGCGCCGGt                                  <  4:205063/149‑1 (MQ=255)                                                                                                                 cAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAGACCCACCTGGGCGCCGGTa                                 <  7:42509/149‑1 (MQ=255)                                                                                                                  aaCTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAGACCCACCTGGGCGCCGGTAt                                <  3:226439/149‑1 (MQ=255)                                                                                                                     tACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGc                                                                                     <  3:136784/93‑1 (MQ=255)                                                                                                                     tACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGc                                                                                     >  4:136784/1‑93 (MQ=255)                                                                                                                       ccGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAGACCCACCTGGGCGCCGGTATGGcc                            >  5:181270/1‑148 (MQ=255)                                                                                                                       ccGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAGACCCACCTGGGCGCCGGTATGGc                             <  4:315473/147‑1 (MQ=255)                                                                                                                        cGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAGACCCACCTGGGCGCCGGTATGGCCaa                          >  5:32301/1‑149 (MQ=255)                                                                                                                              gAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAGACCCACCTGGGCGCCGGTATGGCCAACGAGc                     <  3:129681/148‑1 (MQ=255)                                                                                                                              gAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAGACCCACCTGGGCGCCGGTATGGCCAACGAGc                     >  4:129681/1‑148 (MQ=255)                                                                                                                              gAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAGACCCACCTGGGCGCCGGTATGGCCAACGAGCt                    <  3:279638/149‑1 (MQ=255)                                                                                                                               aGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAGACCCACCTGGGCGCCGGTATGGCCAACGAGCt                    >  1:195198/1‑148 (MQ=255)                                                                                                                                 cACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAGACCCACCTGGGCGCCGGTATGGCCAACGAGCTGAt                 <  6:32301/149‑1 (MQ=255)                                                                                                                                     taGGCCAGGGCCTTGCGCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAGACCCACCTGGGCGCCGGTATGGCCAACGAGCTGATgcg              >  2:299157/2‑148 (MQ=255)                                                                                                                                      aGGCCAGGGCCTTGCGCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAGACCCACCTGGGCGCCGGTATGGCCAACGAGCTGATgcgc             >  1:38645/1‑148 (MQ=255)                                                                                                                                      aGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGATATCGAGCGCACCGGTCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAGACCCACCTGGGCGCCGGTATGGCCAACGAGCTGATgcgc             <  8:88699/148‑1 (MQ=255)                                                                                                                                       ggCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAGACCCACCTGGGCGCCGGTATGGCCAACGAGCTGATGCGCaa           <  1:57091/149‑1 (MQ=255)                                                                                                                                        gCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAGACCCACCTGGGCGCCGGTATGGCCAACGAGCTGATGCGCaa           >  1:335176/1‑148 (MQ=255)                                                                                                                                          cAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAGACCCACCTGGGCGCCGGTATGGCCAACGAGCTGATGCGCAacac        >  4:283320/1‑149 (MQ=255)                                                                                                                                           aGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAGACCCACCTGGGCGCCGGTATGGCCAACGAGCTGATGCGCAACAcc       >  4:108631/1‑149 (MQ=255)                                                                                                                                            gcgcAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAGACCCACCTGGGCGCCGGTATGGCCAACGAGCTGATGCGCAACACCt      <  5:253543/149‑1 (MQ=255)                                                                                                                                                 aGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAGACCCACCTGGGCGCCGGTATGGCCAACGAGCTGATGCGCAACACCTCGGc  >  8:103031/1‑148 (MQ=255)                                                                                                                                                 |                                                                                                                                                    CCGAGCGCCAGGCCAAGCAACAGGCCGAGGAAACCCGCCTGAAGCGCGAGGCCATGACCCGCAAGCTGGGTTCAGCGCTGATACCGAAGCGTTTCGCCGAGCGAACCCTGGCCAACTACCGTGTCGAGCACGAAGGCCAGCGCAAGGCCCTGGCCTACTGCACGCGTTACGTGGCCGCGTTCGAGGAGATCGAGCGCACCGGGCGTTGCCTGATGCTGCTGGGCAGGGTCGGCACCGGCAAGACCCACCTGGGCGCCGGTATGGCCAACGAGCTGATGCGCAACACCTCGGC  >  NC_002947/1751652‑1751943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑