Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,786,669	A→T	9.8%	K167M (AAG→ATG)	tsf →	elongation factor Ts

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,786,669	0	A	T	9.8%	143.3 / 5.8	51	K167M (AAG→ATG)	tsf	elongation factor Ts
Reads supporting (aligned to +/- strand):  ref base A (23/23);  new base T (2/3);  total (25/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.58e-01

CTCGTGAAGCCCTGGTTGCCAAGTGCGGCGAGAACGTCAACATCCGTCGCCTGGTGCGCGTTGAGGGTGACGTTGTCGGTGCCTACCTGCACGGCAACAAGATCGGCGCTGTCGTCGTTCTGAAAGGCGGTGA‑CGTGGAGCTGGCGAAGAACATCGCCATGCACGTTGCAGCTTCGAACCCTGAGTTCCTGGATTCGTCGGAAATCTCCGCCGAGGCCATCGAGCGCGAGAAGAATGTCTTCCTGCAGCTGAACGCCGACAAGATCGCCGGCAAGCCGGAAAACATCGTTGAGAAC  >  NC_002947/1786522‑1786817                                                                                                                                                     |                                                                                                                                                     ctCGTGAAGCCCTGGTTGCCAAGTGCGGCGAGAACGTCAACATCCGTCGCCTGGTGCGCGTTGAGGGTGACGTTGTCGGTGCCTACCTGCACGGCAACAAGATCGGCGCTGTCGTCGTTCTGAAAGGCGGTGA‑CGTGGAGCTGGCgaa                                                                                                                                                      <  2:327334/148‑1 (MQ=255)  tCGTGAAGCCCTGGTTGCCAAGTGCGGCGAGAACGTCAACATCCGTCGCCTGGTGCGCGTTGAGGGTGACGTTGTCGGTGCCTACCTGCACGGCAACAAGATCGGCGCTGTCGTCGTTCTGAAAGGCGGTGA‑CGTGGAGCTGGCgaag                                                                                                                                                     >  8:171077/1‑148 (MQ=255)     tGAAGCCCTGGTTGCCAAGTGCGGCGAGAACGTCAACATCCGTCGCCTGGTGCGCGTTGAGGGTGACGTTGTCGGTGCCTACCTGCACGGCAACAAGATCGGCGCTGTCGTCGTTCTGAAAGGCGGTGA‑CGTGGAGCTGGCGAAGAAc                                                                                                                                                  >  4:176673/1‑148 (MQ=255)          cccTGGTTGCCAAGTGCGGCGAGAACGTCAACATCCGTCGCCTGGTGCGCGTTGAGGGTGACGTTGTCGGTGCCTACCTGCACGGCAACAAGATCGGCGCTGTCGTCGTTCTGAAAGGCGGTGA‑CGTGGAGCTGGCGAAGAACATCGc                                                                                                                                             >  3:91833/1‑148 (MQ=255)          cccTGGTTGCCAAGTGCGGCGAGAACGTCAACATCCGTCGCCTGGTGCGCGTTGAGGGTGACGTTGTCGGTGCCTACCTGCACGGCAACAAGATCGGCGCTGTCGTCGTTCTGAAAGGCGGTGA‑CGTGGAGCTGGCGAAGAACATCGc                                                                                                                                             >  1:154826/1‑148 (MQ=255)               gTTGCCAAGTGCGGCGAGAACGTCAACATCCGTTGCCTGGTGCGCGTTGAGGGTGACGTTGTCGGTGCCTACCTGCACGGCAACAAGATCGGCGCTGTCGTCGTTCTGAAAGGCGGTGA‑CGTGGAGCTGGCGATGTTCTTCGCCagc                                                                                                                                         <  8:33540/147‑3 (MQ=255)                    cAAGTGCGGCGAGAACGTCAACATCCGTCGCCTGGTGCGCGTTGAGGGTGACGTTGTCGGTGCCTACCTGCACGGCAACAAGATCGGCGCTGTCGTCGTTCTGAAAGGCGGTGA‑CGTGGAGCTGGCGAAGAACATCGCCATGCACGTTg                                                                                                                                  >  5:319027/1‑149 (MQ=255)                    cAAGTGCGGCGAGAACGTCAACATCCGTCGCCTGGTGCGCGTTGAGGGTGACGTTGTCGGTGCCTACCTGCACGGCAACAAGATCGGCGCTGTCGTCGTTCTGAAAGGCGGTGA‑CGTGGAGCTGGCGAAGAACATCGCCATGCACGTTg                                                                                                                                  >  4:110330/1‑149 (MQ=255)                        tGCGGCGAGAACGTCAACATCCGTCGCCTGGTGCGCGTTGAGGGTGACGTTGTCGGTGCCTACCTGCACGGCAACAAGATCGGCGCTGTCGTCGTTCTGAAAGGCGGTGA‑CGTGGAGCTGGCGAAGAACATCGCCATGCACGTTGCAGc                                                                                                                              >  2:12143/1‑149 (MQ=255)                           ggcgAGAACGTCAACATCCGTCGCCTGGTGCGCGTTGAGGGTGACGTTGTCGGTGCCTACCTGCACGGCAACAAGATCGGCGCTGTCGTCGTTCTGAAAGGCGGTGA‑CGTGGAGCTGGCGAAGAACATCGCCATGCACGTTGCAGCTTc                                                                                                                           <  7:171077/149‑1 (MQ=255)                              gagaACGTCAACATCCGTCGCCTGGTGCGCGTTGAGGGTGACGTTGTCGGTGCCTACCTGCACGGCAACAAGATCGGCGCTGTCGTCGTTCTGAAAGGCGGTGA‑CGTGGAGCTGGCGAAGAACATCGCCATGCACGTTGCAGCTTCGaa                                                                                                                        >  1:328706/1‑149 (MQ=255)                                gaACGTCAACATCCGTCGCCTGGTGCGCGTTGAGGGTGACGTTGTCGGTGCCTACCTGCACGGCAACAAGATCGGCGCTGTCGTCGTTCTGAAAGGCGGTGA‑CGTGGAGCTGGCGAAGAACATCGCCATGCACGTTGCAGCTTCGAAcc                                                                                                                      >  3:289638/1‑149 (MQ=255)                                  aCGTCAACATCCGTCGCCTGGTGCGCGTTGAGGGTGACGTTGTCGGTGCCTACCTGCACGGCAACAAGATCGGCGCTGTCGTCGTTCTGAAAGGCGGTGA‑CGTGGAGCTGGCGAAGAACATCGCCATGCACGTTGCAGCTTCGAACCCt                                                                                                                    <  8:176723/149‑1 (MQ=255)                                   cGTCAACATCCGTCGCCTGGTGCGCGTTGAGGGTGACGTTGTCGGTGCCTACCTGCACGGCAACAAGATCGGCGCTGTCGTCGTTCTGAAAGGCGGTGA‑CGTAGAGCTGGCGAAGAACATCGCCATGCACGTTGTAGCTTCGAACCCt                                                                                                                    >  4:319115/1‑148 (MQ=255)                                   cGTCAACATCCGTCGCCTGGTGCGCGTTGAGGGTGACGTTGTCGGTGCCTACCTGCACGGCAACAAGATCGGCGCTGTCGTCGTTCTGAAAGGCGGTGA‑CGTAGAGCTGGCGAAGAACATCGCCATGCACGTTGTAGCTTCGAACCCt                                                                                                                    <  3:319115/148‑1 (MQ=255)                                    gTCAACATCCGTCGCCTGGTGCGCGTTGAGGGTGACGTTGTCGGTGCCTACCTGCACGGCAACAAGATCGGCGCTGTCGTCGTTCTGAAAGGCGGTGA‑CGTGGAGCTGGCGAAGAACATCGCCATGCACGTTGCAGCTTCGAACCCt                                                                                                                    >  1:19737/1‑147 (MQ=255)                                    gTCAACATCCGTCGCCTGGTGCGCGTTGAGGGTGACGTTGTCGGTGCCTACCTGCACGGCAACAAGATCGGCGCTGTCGTCGTTCTGAAAGGCGGTGA‑CGTGGAGCTGGCGAAGAACATCGCCATGCACGTTGCAGCTTCAAACCCt                                                                                                                    >  3:301806/1‑147 (MQ=255)                                      cAACATCCGTCGCCTGGTGCGCGTTGAGGGTGACGTTGTCGGTGCCTACCTGCACGGCAACAAGATCGGCGCTGTCGTCGTTCTGAAAGGCGGTGACCGTGGAGCTGGCGAAGAACATCGCCATGc                                                                                                                                        <  1:20682/126‑1 (MQ=255)                                      cAACATCCGTCGCCTGGTGCGCGTTGAGGGTGACGTTGTCGGTGCCTACCTGCACGGCAACAAGATCGGCGCTGTCGTCGTTCTGAAAGGCGGTGA‑CGTGGAGCTGGCGAAGAACATCGCCATGc                                                                                                                                        >  2:20682/1‑125 (MQ=255)                                       aaCATCCGTCGCCTGGTGCGCGTTGAGGGTGACGTTGTCGGTGCCTACCTGCACGGCAACAAGATCGGCGCTGTCGTCGTTCTGAAAGGCGGTGA‑CGTGGAGCTGGCGAAGAACATCGCCATGCACGTTGCAGCTTCGAACCCTGAGtt                                                                                                               >  2:345638/1‑149 (MQ=255)                                          aTCCGTCGCCTGGTGCGCGTTGAGGGTGACGTTGTCGGTGCCTACCTGCACGGCAACAAGATCGGCGCTGTCGTCGTTCTGAAAGGCGGTGA‑CGTGGAGCTGGCGAAGAACATCGCCATGCACGTTGCAGCTTCGAACCCTGAGTTCCt                                                                                                            <  4:289638/149‑1 (MQ=255)                                             cGTCGCCTGGTGCGCGTTGAGGGTGACGTTGTCGGTGCCTACCTGCACGGCAACAAGATCGGCGCTGTCGTCGTTCTGAAAGGCGGTGA‑CGTGGAGCTGGCGAAGAACATCGCCATGCACGTTGCAGCTTCGAACCCTGAGTTCCTgg                                                                                                          <  1:345638/148‑1 (MQ=255)                                                      gTGCGCGTTGAGGGTGACGTTGTCGGTGCCTACCTGCACGGCAACAAGATCGGCGCTGTCGTCGTTCTGAAAGGCGGTGA‑CGTGGAGCTGGCGAAGAACATCGCCATGCACGTTGCAGCTTCGAACCCTGAGTTCCTGGATTCGTCGGa                                                                                                <  3:176673/149‑1 (MQ=255)                                                        gcgcGTTGAGGGTGACGTTGTCGGTGCCTACCTGCACGGCAACAAGATCGGCGCTGTCGTCGTTCTGAAAGGCGGTGA‑CGTGGAGCTGGCGAAGAACATCGCCATGCACGTTGCAGCTTCGAACCCTGAGTTCCTGGATTCGTCGGaaa                                                                                              >  5:25117/1‑149 (MQ=255)                                                               gAGGGTGACGTTGTCGGTGCCTACCTGCACGGCAACAAGATCGGCGCTGTCGTCGTTCTGAAAGGCGGTGA‑CGTGGAGCTGGCGATGAACATCGCCATGCACGTTGCAGCTTCGAACCCTGAGTTCCt                                                                                                            >  1:145545/1‑128 (MQ=255)                                                               gAGGGTGACGTTGTCGGTGCCTACCTGCACGGCAACAAGATCGGCGCTGTCGTCGTTCTGAAAGGCGGTGA‑CGTGGAGCTGGCGATGAACATCGCCATGCACGTTGCAGCTTCGAACCCTGAGTTCCt                                                                                                            <  2:145545/128‑1 (MQ=255)                                                                aGGGTGACGTTGTCGGTGCCTACCTGCACGGCAACAAGATCGGCGCTGTCGTCGTTCTGAAAGGCGGTGA‑CGTGGAGCTGGCGAAGAACATCGCCATGCACGTTGCAGCTTCGAACCCTGAGTTCCTGGATTCGTCGGAAATCTccgc                                                                                       <  6:25117/148‑1 (MQ=255)                                                                     gACGTTGTCGGTGCCTACCTGCACGGCAACAAGATCGGCGCTGTCGTAGTTCTGAAAGGCGGTGA‑CGTGGAGCTGGCGAAGAACATCGCCATGCACGTTGCAGCTTCGAACCCTGAGTTCCTGGATTCGTCGGAAATCTCCGCCGAGGc                                                                                 >  4:312174/1‑149 (MQ=255)                                                                        gTTGTCGGTGCCTACCTGCACGGCAACAAGATCGGCGCTGTCGTCGTTCTGAAAGGCGGTGA‑CGTGGAGCTGGCGATGTTCTTCGCCagc                                                                                                                                         <  3:163099/90‑3 (MQ=255)                                                                        gTTGTCGGTGCCTACCTGCACGGCAACAAGATCGGCGCTGTCGTCGTTCTGAAAGGCGGTGA‑CGTGGAGCTGGCGATGTTCTTCGCCagc                                                                                                                                         >  4:163099/1‑88 (MQ=255)                                                                              ggTGCCTACCTGCACGGCAACAAGATCGGCGCTGTCGTCGTTCTGAAAGGCGGTGA‑CGTGGAGCTGGCGAAGAACATCGCCa                                                                                                                                           >  1:57591/1‑82 (MQ=255)                                                                              ggTGCCTACCTGCACGGCAACAAGATCGGCGCTGTCGTCGTTCTGAAAGGCGGTGA‑CGTGGAGCTGGCGAAGAACATCGCCa                                                                                                                                           <  2:57591/82‑1 (MQ=255)                                                                              ggTGCCTACCTGCACGGCAACAAGATCGGCGCTGTCGTCGTTCTGAAAGGCGGTGA‑CGTGGAGCTGGCGAAGAACATCGCCATGCACGTTGCAGCTTCGAACCCTGAGTTCCTGGATTCGTCGGAAATCTccgc                                                                                       >  6:226513/1‑134 (MQ=255)                                                                              ggTGCCTACCTGCACGGCAACAAGATCGGCGCTGTCGTCGTTCTGAAAGGCGGTGA‑CGTGGAGCTGGCGAAGAACATCGCCATGCACGTTGCAGCTTCGAACCCTGAGTTCCTGGATTCGTCGGAAATCTccgc                                                                                       <  5:226513/134‑1 (MQ=255)                                                                                tGCCTACCTGCACGGCAACAAGATCGGCGCTGTCGTCGTTCTGAAAGGCGGTAA‑CGTGTAGCTGGCGAAGAACATCGCCATGCACGTTGCAGCTTCGAACCCTGAGTTCCTGGATTCGTCGGAAATCTCCGCCGAGGCCATCGAGcgcg                                                                      <  7:188398/149‑1 (MQ=255)                                                                                   cTACCTGCACGGCAACAAGATCGGCGCTGTCGTCGTTCTGAAAGGCGGTGA‑CGTGGAGCTGGCGAAGAACATCGCCATGCACGTTGCAGCTTCGAACCCTGAGTTCCTGGATTCGTCGGAAATCTCCGCCGAGGCCATc                                                                             >  2:152019/1‑139 (MQ=255)                                                                                   cTACCTGCACGGCAACAAGATCGGCGCTGTCGTCGTTCTGAAAGGCGGTGA‑CGTGGAGCTGGCGAAGAACATCGCCATGCACGTTGCAGCTTCGAACCCTGAGTTCCTGGATTCGTCGGAAATCTCCGCCGAGGCCATc                                                                             <  1:152019/139‑1 (MQ=255)                                                                                   cTACCTGCACGGCAACAAGATCGGCGCTGTCGTCGTTCTGAAAGGCGGTGA‑CGTGGAGCTGGCGAAGAACATCGCCATGCACGTTGCAGCTTCGAACCCTGAGTTCCTGGATTCGTCGGAAATCTCCGCCGAGGCCATCGAGCGCgag                                                                    >  8:261530/1‑148 (MQ=255)                                                                                      ccTGCACGGCAACAAGATCGGCGCTGTCGTCGTTCTGAAAGGCTGTGA‑CGTGGAGCTGGCGAAGAACATCGCCATGCACGTTGCAGCTTCGAACCCTGAGTTCCTGGATTCGTCGGAAATCTCCGCCGAGGCCATCTAGCGCGagaag                                                                 <  3:312174/148‑1 (MQ=255)                                                                                          cACGGCAACAAGATCGGCGCTGTCGTCGTTCTGAAAGGCGGTGA‑CGTGGAGCTGGCGAAGAACATCGCCATGCACGTTGCAGCTTCGAACCCTGAGTTCCTGGATTCGTCGGaa                                                                                               <  1:207141/114‑1 (MQ=255)                                                                                          cACGGCAACAAGATCGGCGCTGTCGTCGTTCTGAAAGGCGGTGA‑CGTGGAGCTGGCGAAGAACATCGCCATGCACGTTGCAGCTTCGAACCCTGAGTTCCTGGATTCGTCGGaa                                                                                               >  2:207141/1‑114 (MQ=255)                                                                                          cACGGCAACAAGATCGGCGCTGGCGTCGTTCTGAAAGGCGGTGA‑CGTGGAGCTGGCGAAGAACATCGCCATGCACGTTGCAGCTTCGAACCCCGAGTTCCTGGATTCGTCGGAAATCTCCGCCGAGGCCATCGAGCGCGAGAAGAAt                                                              <  3:105904/147‑1 (MQ=255)                                                                                           aCGGCAACAAGATCGGCGCTGTCGTCGTTCTGAAAGGCGGTGA‑CGTGGAGCTGGCGAAGAACATCGCCATGCACGTTGCAGCTTCGAACCCTGAGTTCCTGGATTCGTCGGAAATCTCCGCCGAGGCCATCGAGCGCGAGAAGAATGTc                                                           <  7:261530/149‑1 (MQ=255)                                                                                             ggCAACAAGATCGGCGCTGTCGTCGTTCTGAAAGGCGGTGA‑CGTGGAGCTGGCGAAGAACATCGCCATGCACGTTGCAGCTTCGAACCCTGAGTTCCTGGATTCGTCGGAAATCTccgcc                                                                                      >  4:281295/1‑120 (MQ=255)                                                                                             ggCAACAAGATCGGCGCTGTCGTCGTTCTGAAAGGCGGTGA‑CGTGGAGCTGGCGAAGAACATCGCCATGCACGTTGCAGCTTCGAACCCTGAGTTCCTGGATTCGTCGGAAATCTccgcc                                                                                      <  3:281295/120‑1 (MQ=255)                                                                                                aacaaGATCGGCGCTGTCGTCGTTCTGAAAGGCGGTGA‑CGTGGAGCTGGCGAAGAACATCGCCATGCACGTTGCAGCTTCGAACCCTGAGTTCCTGGATTCGTCGGAAATCTCCGCCGAGGCCATCGAGCGCGAGAAGAATGTCTTCCt                                                      <  3:110330/149‑1 (MQ=255)                                                                                                      aTCGGCGCTGTCGTCGTTCTGAAAGGCGGTGA‑CGTGGAGCTGGCGAAGAACATCGCCATGCACGTTGCAGCTTCGAACCCTGAGTTCCTGGATTCGTCGGAAATCTCCGCCGAGGCCATCGAGCGCGAGAAGAATGTCTTCCTGCAGCt                                                <  6:319027/149‑1 (MQ=255)                                                                                                                tcgtcgTTCTGAAAGGCGGTGA‑CGTGGAGCTGGCGAAGAACATCGCCATGCACGTTGCAGCTTCGAACCCTGAGTTCCTGGATTCGTCGGAAATCTCCGCCGAGGCCATCGAGCGCGAGAAGAATGTCTTCCTGCAGCTGAACGCCGa                                       <  1:12143/148‑1 (MQ=255)                                                                                                                tcgtcgTTCTGAAAGGCGGTGA‑CGTGGAGCTGGCGAAGAACATCGCCATGCACGTTGCAGCTTCGAACCCTGAGTTCCTGGATTCGTCGGAAATCTCCGCCGAGGCCATCGAGCGCGAGAAGAATGTCTTCCTGCAGCTGAACGCCGAc                                      <  2:19737/149‑1 (MQ=255)                                                                                                                               gCGGTGA‑CGTGGAGCTGGCGAAGAACATCGCCATGCACGTTGCAGCTTCGAACCCTGAGTTCCTGGATTCGTCGGAAATCTCCGCCGAGGCCATCGAGCGCGAGAAGAATGTCTTCCTGCAGCTGAACGCCGACAAGATCGCCGGCAAg                       <  2:154826/149‑1 (MQ=255)                                                                                                                                     a‑CGTGGAGCTGGCGAAGAACATCGCCATGCACGTTGCAGCTTCGAACCCTGAGTTCCTGGATTCGTCGGAAATCTCCGCCGAGGCCATCGAGCGCGAGAAGAATGTCTTCCTGCAGCTGAACGCCGACAAGATCGCCGGCAAGCCGGaa                 <  4:91833/149‑1 (MQ=255)                                                                                                                                          ggAGCTGGCGAAGAACATCGCCATGCACGTTGCAGCTTCGAACCCTGAGTTCCTGGATTCGTCGGAAATCTCCGCCGAGGCCATCGAGCGCGAGAAGAATGTCTTCCTGCAGCTGAACGCCGACAAGATCGCCGGCAAGCCGGAAAACa             >  2:98837/1‑149 (MQ=255)                                                                                                                                              cTGGCGAAGAACATCGCCATGCACGTTGCAGCTTCGAACCCTGAGTTCCTGGATTCGTCGGAAATCTCCGCCGAGGCCATCGAGCGCGAGAAGAATGTCTTCCTGCAGCTGAACGCCGACAAGATCGCCGGCAAGCCGGAAAACATCGt         >  7:178570/1‑149 (MQ=255)                                                                                                                                                   gaagaaCATCGCCATGCACGTTGCAGCTTCGAACCCTGAGTTCCTGGATTCGTCGGAAATCTCCGCCGAGGCCATCGAGCGCGAGAAGAATGTCTTCCTGCAGCTGAACGCCGACAAGATCGCCGGCAAGcc                     <  4:241748/132‑1 (MQ=255)                                                                                                                                                   gaagaaCATCGCCATGCACGTTGCAGCTTCGAACCCTGAGTTCCTGGATTCGTCGGAAATCTCCGCCGAGGCCATCGAGCGCGAGAAGAATGTCTTCCTGCAGCTGAACGCCGACAAGATCGCCGGCAAGcc                     >  3:241748/1‑132 (MQ=255)                                                                                                                                                   gaagaaCATCGCCATGCACGTTGCAGCTTCGAACCCTGAGTTCCTGGATTCGTCGGAAATCTCCGCCGAGGCCATCGAGCGCGAGAAGAATGTCTTCCTGCAGCTGAACGCCGACAAGATCGCCGGCAAGCCGGAAAACATCGTTgag     >  8:301394/1‑148 (MQ=255)                                                                                                                                                     agaaCATCGCCATGCACGTTGCAGCTTCGAACCCTGAGTTCCTGGATTCGTCGGAAATCTCCGCCGAGGCCATCGAGCGCGAGAAGAATGTCTTCCTGCAGCTGAACGCCGACAAGATCGCCGGCAAGCCGGAAAACATCGTTGAGAAc  <  7:301394/149‑1 (MQ=255)                                                                                                                                                     agaaCATCGCCATGCACGTTGCAGCTTCGAACCCTGAGTTCCTGGATTCGTCGGAAATCTCCGCCGAGGCCATCGAGCGCGAGAAGAATGTCTTCCTGCAGCTGAACGCCGACAAGATCGCCGGCAAGCCGGAAAACATCGTTGAGAAc  <  2:328706/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                     CTCGTGAAGCCCTGGTTGCCAAGTGCGGCGAGAACGTCAACATCCGTCGCCTGGTGCGCGTTGAGGGTGACGTTGTCGGTGCCTACCTGCACGGCAACAAGATCGGCGCTGTCGTCGTTCTGAAAGGCGGTGA‑CGTGGAGCTGGCGAAGAACATCGCCATGCACGTTGCAGCTTCGAACCCTGAGTTCCTGGATTCGTCGGAAATCTCCGCCGAGGCCATCGAGCGCGAGAAGAATGTCTTCCTGCAGCTGAACGCCGACAAGATCGCCGGCAAGCCGGAAAACATCGTTGAGAAC  >  NC_002947/1786522‑1786817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑