Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,810,336 C→A 9.8% intergenic (+97/‑72) eno → / → ftsB enolase/cell division protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,810,3360CA 9.8% 127.4 / 4.5 48intergenic (+97/‑72)eno/ftsBenolase/cell division protein
Reads supporting (aligned to +/- strand):  ref base C (19/24);  new base A (2/3);  total (21/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

GGCGCCAAAGCGGTTTACCGCGGTCGTGCCGAGTTTCGCGGCTAAGCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGTGGCCTGCAGTACCGCCTGTGGGTGG  >  NC_002947/1810195‑1810480
                                                                                                                                             |                                                                                                                                                
ggCGCCAAAGCGGTTTACCGCGGTCGTGCCGAGTTTCGCGGCTAAGCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAg                                                                                                                                            <  4:169765/148‑1 (MQ=255)
 gcgcCAAAGCGGTTTACCGCGGTCGTGCCGAGTTTCGCGGCTAAGCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCGAGGCCAGGc                                                                                                                                          <  6:114152/149‑1 (MQ=255)
     cAAAGCGGTTTACCGCGGTCGTGCCGAGTTTCGCGGCTAAGCAAGAGATGGTAAAAAGGCAGCAGCCGGGGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCg                                                                                                                                      >  3:284005/1‑149 (MQ=255)
      aaaGCGGTTTACCGCGGTCGTGCCGAGTTTCGCGGCTAAGCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCGAGGCCAGGCTTCGt                                                                                                                                     <  1:257554/149‑1 (MQ=255)
        aGCGGTTTACCGCGGTCGTGCCGAGTTTCGCGGCTAAGCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCGAGGCCAGGCTTCGtcg                                                                                                                                   <  6:344489/149‑3 (MQ=255)
             tttACCGCGGTCGTGCCGAGTTTCGCGGCTAAGCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTc                                                                                                                              <  5:354315/149‑1 (MQ=255)
             tttACCGCGGTCGTGCCGAGTTTCGCGGCTAAGCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTc                                                                                                                              <  8:153061/149‑1 (MQ=255)
             tttACCGCGGTCGTGCCGAGTTTCGCGGCTAAGCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTc                                                                                                                              <  8:157345/149‑1 (MQ=255)
                  cgcgGTCGTGCCGAGTTTCGCGGCTAAGCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGc                                                                                                                         >  4:148941/1‑149 (MQ=255)
                   gcgGTCGTGCCGAGTTTCGCGGCTAAGCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGcc                                                                                                                        <  2:115712/149‑1 (MQ=255)
                   gcgGTCGTGCCGAGTTTCGCGGCTAAGCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGcc                                                                                                                        <  5:320932/149‑1 (MQ=255)
                    gtgTCGTGCCGAGTTTCGGGGGTAAGGAAGATATGTTAAAAAGGCAGCAGAGGGAGTTGTAGGAACGTTCTTACTGAACTTTCCTACGTAGTAACGGGTTTCTTTCGACGAAGCCTGGCCTCGGCGAGGCTTCGTGCTATTCGAGGccg                                                                                                                       <  4:273054/147‑2 (MQ=255)
                      gTCGTGCCGAGTTTCGCGGCTAAGCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCGTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGcccc                                                                                                                      >  7:241401/1‑148 (MQ=255)
                         gTGCCGAGTTTCGCGGCTAAGCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGaa                                                                                                                   <  4:139882/148‑1 (MQ=255)
                           gCCGAGTTTCGCGGCTAAGCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTAGAGGCCACGAAGt                                                                                                                 >  1:39693/1‑148 (MQ=255)
                            ccGAGTTTCGCGGCTAAGCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTaa                                                                                                               >  6:95750/1‑149 (MQ=255)
                             cGAGTTTCGCGGCTAAGCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTaa                                                                                                               >  7:34017/1‑148 (MQ=255)
                             cGAGTTTCGCGGCTAAGCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAAc                                                                                                              <  5:95750/149‑1 (MQ=255)
                                                 gagaTGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGc                                                                                                                                               >  7:53396/1‑96 (MQ=255)
                                                 gagaTGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGc                                                                                                                                               <  8:53396/96‑1 (MQ=255)
                                                 gagaTGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCtttt                                                                                           >  8:96546/1‑148 (MQ=255)
                                                       gTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTg                                                                                    <  8:34017/149‑1 (MQ=255)
                                                             aGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTg                                                                                    <  7:194064/143‑1 (MQ=255)
                                                             aGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCAGGCCGCGGCCAGGCGTAGTGCTATTCGAGGCCCCCAACAAAAAAATAACCGGCACCCGTTTTAAACGGGaga                                                                                >  8:194064/1‑145 (MQ=255)
                                                                  gcagcCGGGGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGAt                                                                         <  4:284005/149‑1 (MQ=255)
                                                                     gcCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATgcg                                                                      <  3:148941/149‑1 (MQ=255)
                                                                                            aCTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCg                                               >  1:264119/1‑149 (MQ=255)
                                                                                             cTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAAc                                                                                       <  2:238174/108‑1 (MQ=255)
                                                                                             cTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAAc                                                                                       >  1:238174/1‑108 (MQ=255)
                                                                                              tGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCAGGCCTCGGCCAGGCTTAGTGCTATTAGAGGCCCCCAAGTAACAAATACGCGGCAGCCATTTTAACCTGGATAACGTGATGCGCAGTACCTATTTGTTGTTGCTCGt                                              >  4:143873/1‑148 (MQ=255)
                                                                                                   tttCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTAGGCCAGGCTTCGTGCTATTAGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTTACCTGGATAAAGTGATGCGCAGTCCCTATTGGTTTTTGCTCGTTCTg                                          >  4:327666/1‑147 (MQ=255)
                                                                                                        tACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTGGTGCGATTCGAGGCCCCGAAGTAACAAGTAAGCCGCAGCCGTTTTAACCGGGATAACGTGATGCGCAGTCCCGATTGGTTGTTGCGCGTTGTGCTCtttg                                   >  8:30184/1‑147 (MQ=255)
                                                                                                           gTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGt                                >  4:93059/1‑149 (MQ=255)
                                                                                                            tACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGtt                               <  2:264119/149‑1 (MQ=255)
                                                                                                               aaaCGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCtt                                                                                             <  8:46496/83‑1 (MQ=255)
                                                                                                                 aCGGGTTTCTGTCGACGAAGCCTGGCCTCCGCCAGGCGTCGTGGTATTCGAGGCCCCGAAGTAACAAGTAAGCGGCAGCCGTTTTTACCTGGATAACGTGATGCCCCGTCCCGATTGGTTGTTACTCGTTCTGGGATTGCTGTTggggg                          >  8:127540/1‑147 (MQ=255)
                                                                                                                   gggTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGTGGc                        <  7:96546/149‑1 (MQ=255)
                                                                                                                              gacgaAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGTGGCCTGCAGTAcc              <  8:241401/148‑1 (MQ=255)
                                                                                                                              cacgaAGCCTGGCCGAGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGTGGCCTGCAGTACCg             >  8:88783/2‑149 (MQ=255)
                                                                                                                               acgaAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGTGGCCTGCAGTACCGc            >  7:48774/1‑149 (MQ=255)
                                                                                                                                   aGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGTGGCCTGCAGTACCGCCtgt        <  2:39693/149‑1 (MQ=255)
                                                                                                                                    gCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGTGGCCTGCAGTACCGCCtgt        <  5:14739/148‑1 (MQ=255)
                                                                                                                                    gCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGTGGCCTGCAGTACCGCCtgt        >  6:14739/1‑148 (MQ=255)
                                                                                                                                      cTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGTGGCCTGCAGTACCGCCTgtggg     >  8:315930/1‑149 (MQ=255)
                                                                                                                                         gCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGTGGCCTGCAGTACCGCCTgtgggtgg  <  3:327666/149‑1 (MQ=255)
                                                                                                                                          ccTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTg                                    <  5:344361/114‑1 (MQ=255)
                                                                                                                                          ccTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTg                                    >  6:344361/1‑114 (MQ=255)
                                                                                                                                           cTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGtt                               <  6:132822/118‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                
GGCGCCAAAGCGGTTTACCGCGGTCGTGCCGAGTTTCGCGGCTAAGCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTACTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGTGGCCTGCAGTACCGCCTGTGGGTGG  >  NC_002947/1810195‑1810480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑