Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,811,637 T→A 10.7% N261Y (AAT→TAT)  PP_1615 ← LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,811,6370TA10.7% 149.7 / 8.2 56N261Y (AAT→TAT) PP_1615LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (23/27);  new base A (3/3);  total (26/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.95e-01

AGAGGCCAGTACCAGCAGCACATCCCGTCAGCCGAAACGATACTCCGGCAACCCCGCCAACCCCTCCTTCAGGTAATCCACCAGCCGCCGTACCTTCGGCGACAGGTGCCTCTGCTGCGGATACAGCGCCCATACCGCCGTATTCGGCGGTTGGTGCG‑CCTCCAGCAGCGACACCAGCGCACCACTGTTCAGGTGTTCCAGCACGTAGTAATCCGGCAACTGGCACAACCCGATTCCTTGCAGCGCCGCATCCAGCACTGCCTGGCCACTGTTGCAGCGCCAGTTACCCTG  >  NC_002947/1811494‑1811784
                                                                                                                                               |                                                                                                                                                    
agagGCCAGTACCAGCAGCACATCCCGTCAGCCGAAACGATACTCCGGCAACCCCGCCAACCCCTCCTTCAGGTAATCCACCAGCCGCCGTACCTTCGGCGACAGGTGCCTCTGCTGCGGATACAGCGCCCATACCGCCGTATTcggcg                                                                                                                                                 <  6:106267/149‑1 (MQ=255)
  agGCCAGTACCAGCAGCACATCCCGTCAGCCGAAACGATACTCCGGCAACCCCGCCAACCCCTCCTTCAGGTAATCCACCAGCCGCCGTACCTTCGGCGACAGGTGCCTCTGCTGCGGATACAGCGCCCATACCGCCGTATTCGGCGGt                                                                                                                                               >  6:15962/1‑149 (MQ=255)
       aGTACCAGCAGCACATCCCGTCAGCCGAAACGATACTCCGGCAACCCCGCCAACCCCTCCTTCAGGTAATCCACCAGCCGCCGTACCTTCGGCGACAGGTGCCTCTGCTGCGGATACAGCGCCCATACCGCCGTATTCGGCGGTTGGTg                                                                                                                                          >  8:44245/1‑149 (MQ=255)
        gTACCAGCAGCACCTCCCGTCAGCCGAACCGATACTCCGGCAACCCCGCCAACCCCTCCTTCAGGTAATCCACCAGCCGCCGTACCTTCGGCGACAGGTGCCTCTGCTGCGGATACAGCGCACCAACCGCCGAATACGGCGGtatgggc                                                                                                                                         <  7:71301/149‑7 (MQ=255)
            cagcagCACATCCCGTCAGCCGAAACGATACTCCGGCAACCCCGCCAACCCCTCCTTCAGGTAATCCACCAGCCGCCGTACCTTCGGCGACATGTGCCTCTGCTGCGGATACAGCGCCCATACCGCCGTATTCGGCGGTTGGTCCG‑CCt                                                                                                                                    >  7:65946/1‑149 (MQ=255)
            cagcagCACATCCCGTCAGCCGAAACGATACTCCGGCAACCCCGCCAACCCCTCCTTCAGGTAATCCACCAGCCGCCGTACCTTCGGCGACAGGTGCCTCTGCTGCGGATACAGCGCCCATACCGCCGTATTCGGCGGTTGGTGCG‑CCt                                                                                                                                    >  2:274406/1‑149 (MQ=255)
            cagcagCACATCCCGTCAGCCG‑AACGATACTCCGGCAACCCCGCCAACCCCTCCTTCAGGTAATCCACCAGCCGCCGTACCTTCGGCGACAGGTGCCTCTGCTGCGGATACAGCGCCCATACAGCCGAATTCGGCGGTTCGTGCG‑CCTc                                                                                                                                   >  6:121126/1‑149 (MQ=255)
                   acaTCCCGTCAGCCGAAACGATACTCCGGCAACCCCGCCAACCCCTCCTTCAGGTAATCCACCAGCCGCCGTACCTTCGGCGACAGGTGCCTCTGCTGCGGATACAGCGCCCATACCGCCGTATTCGGCGGTTGGTGCG‑CCTCcagcag                                                                                                                             <  8:31228/149‑1 (MQ=255)
                    caTCCCGTCCGCCGAACCGATACGCCGGCAACCCCGCCAACCCCTCCTTCAGGTAATCCACCAGCCGCCGTACCTTCGGCGACAGGTGCCTCTGCTGCGGATACAGCGCCCATACCGCCGTATTCGGCGGTTGGTGCG‑CCTCCtgcagc                                                                                                                            <  8:258418/149‑1 (MQ=255)
                    caTCCCGTCAGCCGAAACGATACTCCGGCAACCCCGCCAACCCCTCCTTCAGGTAATCCACCAGCCGCCGTACCTTCGGCGACAGGTGCCTCTGCTGCGGATACAGCGCCCATACCGCCGTATTCGGCGGTTGGTGCG‑CCTCCagcagc                                                                                                                            <  7:88409/149‑1 (MQ=255)
                              gCCGAAACGATACTCCGGCAACCCCGCCAACCCCTCCTTCAGGTAATCCACCAGCCGCCGTACCTTCGGCGACAGGTGCCTCTGCTGCGGATACAGCGCCCATACCGCCGTATTCGGCGGTTGGt                                                                                                                                           >  6:152075/1‑125 (MQ=255)
                              gCCGAAACGATACTCCGGCAACCCCGCCAACCCCTCCTTCAGGTAATCCACCAGCCGCCGTACCTTCGGCGACAGGTGCCTCTGCTGCGGATACAGCGCCCATACCGCCGTATTCGGCGGTTGGt                                                                                                                                           <  5:152075/125‑1 (MQ=255)
                                 gAAACGATACTCCGGCAACCCCGCCAACCCCTCCTTCAGGTAATCCACCAGCCGCCGTACCTTCGGCGACAGGTGCCTCTGCTGCGGATACAGCGCCCATACCGCCGTATTCGGCGGTTGGTGCG‑CCTCCAGCAGCGACACCAgcgccc                                                                                                               <  1:228761/149‑3 (MQ=255)
                                    aggATAGTCCGGCATCCCCGCCAACCCCTCCTTCAGGTAATCCAACAGCGGCAGTAGCTTCGGCGAGAGGTGCCTCTGCTTCTGATACAGCGCCCATACCGCGGTATTCGGCGGTTGGTGCG‑CCTGCAGCTGCGACACCAGCGcaccac                                                                                                            <  4:12207/147‑1 (MQ=255)
                                      gATACTCCGGCAACCCCGCCAACCCCTCCTTCAGGTAATCCACCAGCCGCCGTACCTTCGGCGACAGGTGCCTCTGCTGCGGATACAGCGCCCATACCGCCGTATTCGGCGGTTGGTGCG‑CCTCCAGCAGCGACACCAGCGCACCACTg                                                                                                          >  5:70650/1‑149 (MQ=255)
                                            ccGGCAACCCCGCCAACCCCTCCTTCAGGTAATCCACCAGCCGCCGTACCTTCGGCGACAGGTGCCTCTGCTGCGGATACAGCGCCCATACCGCCGAATACGGCGGtatg                                                                                                                                            >  1:171890/1‑107 (MQ=255)
                                            ccGGCAACCCCGCCAACCCCTCCTTCAGGTAATCCACCAGCCGCCGTACCTTCGGCGACAGGTGCCTCTGCTGCGGATACAGCGCCCATACCGCCGAATACGGCGGtatg                                                                                                                                            >  1:89275/1‑107 (MQ=255)
                                            ccGGCAACCCCGCCAACCCCTCCTTCAGGTAATCCACCAGCCGCCGTACCTTCGGCGACAGGTGCCTCTGCTGCGGATACAGCGCCCATACCGCCGAATACGGCGGtatg                                                                                                                                            <  2:171890/110‑4 (MQ=255)
                                            ccGGCAACCCCGCCAACCCCTCCTTCAGGTAATCCACCAGCCGCCGTACCTTCGGCGACAGGTGCCTCTGCTGCGGATACAGCGCCCATACCGCCGAATACGGCGGtatg                                                                                                                                            <  2:89275/110‑4 (MQ=255)
                                             cGGCAACCCCGCCAACCCCTCCTTCAGGTAATCCACCAGCCGCCGTACCTTCGGCGACAGGTGCCTCTGCTGCGGATACAGCGCCCATACCGCCGTATTCGGCGGTTGGTGCGCCCTCCAGCAGCGACACCAGCGCACCACTGTTCAg                                                                                                     <  1:41075/148‑1 (MQ=255)
                                                  aCCCCTCCAACCCCTCCTTCAGGTAATCCACCAGCCGCCGTACCTTCGGCGACAGGTGCCTCTGCTGCGGATACAGCGCCCATACCGCCGTATTCGGCGGTTGGTGCG‑CCTCCAGCAGCGACACCAGCGCACCACTGTTCAGGTGTTcc                                                                                              <  5:121126/149‑1 (MQ=255)
                                                    cccGCCAACCCCTCCTTCAGGTAATCCACCAGCCGCCGTACCTTCGGCGACAGGTGCCTCTGCTGCGGATACAGCGCCCATACCGCCGTATTCGGCGGTTGGTGCG‑CCTCCAGCAGCGACACCAGCGCACCACTGTTCAGGTGTTCCAg                                                                                            >  6:247754/1‑149 (MQ=255)
                                                               ctcctTCAGGTAATCCACCAGCCGCCGTACCTTCGGCGACAGGTGCCTCTGCTGCGGATACAGCGCCCATACCGCCGTATTCGGCGGTTGGTGCG‑CCTCCAGCAGCGACACCAGCGCACCACTGTTc                                                                                                       >  7:146813/1‑127 (MQ=255)
                                                               ctcctTCAGGTAATCCACCAGCCGCCGTACCTTCGGCGACAGGTGCCTCTGCTGCGGATACAGCGCCCATACCGCCGTATTCGGCGGTTGGTGCG‑CCTCCAGCAGCGACACCAGCGCACCACTGTTc                                                                                                       <  8:5440/127‑1 (MQ=255)
                                                               ctcctTCAGGTAATCCACCAGCCGCCGTACCTTCGGCGACAGGTGCCTCTGCTGCGGATACAGCGCCCATACCGCCGTATTCGGCGGTTGGTGCG‑CCTCCAGCAGCGACACCAGCGCACCACTGTTc                                                                                                       <  8:146813/127‑1 (MQ=255)
                                                               ctcctTCAGGTAATCCACCAGCCGCCGTACCTTCGGCGACAGGTGCCTCTGCTGCGGATACAGCGCCCATACCGCCGTATTCGGCGGTTGGTGCG‑CCTCCAGCAGCGACACCAGCGCACCACTGTTc                                                                                                       >  7:5440/1‑127 (MQ=255)
                                                               ctcctTCAGGTAATCCACCAGCCGCCGTACCTTCGGCGACAGGTGCCTCTGCTGCGGATACAGCGCCCATACCGCCGTATTCGGCGGTTGGTGCG‑CCTCCAGCAGCGACACCAGCGCACCACTGTTCAGGTGTTCCAGCACGTAGTAAt                                                                                 >  7:191571/1‑149 (MQ=255)
                                                                   ttCAGGTAATCCACCAGCCGCCGTACCTTCGGCGACAGGTGCCTCTGCTGCGGATACAGCGCCCATACCGCCGTATTCGGCGGTTGGTGCG‑CCTCCAGCAGCGACACCAGCGcac                                                                                                               <  2:150784/115‑1 (MQ=255)
                                                                   ttCAGGTAATCCACCAGCCGCCGTACCTTCGGCGACAGGTGCCTCTGCTGCGGATACAGCGCCCATACCGCCGTATTCGGCGGTTGGTGCG‑CCTCCAGCAGCGACACCAGCGcac                                                                                                               >  1:150784/1‑115 (MQ=255)
                                                                        gTAATCCACCAGCCGCCGTACCTTCGGCGACAGGTGCCTCTGCTGCGGATACAGCGCCCATACCGCCGTATTCGGCGGTTGGTGCG‑CCTCCAGCAGCGACACCAGCGCACCACTGTTCAGGTGTTCCAGCACGTAGTAATCCGGCAACt                                                                        >  8:257926/1‑149 (MQ=255)
                                                                         tAATCCACCAGCCGCCGTACCTTCGGCGACAGGTGCCTCTGCTGCGGATACAGCGCCCATACCGCCGTATTCGGCGGTTGGTGCG‑CCTCCAGCAGCGACACCAGCGCACCACTGTTCAGGTGTTCCAGCACGTAGTAATCCGGCAACTg                                                                       <  5:15962/149‑1 (MQ=255)
                                                                             ccaccaGCCGCCGTACCTTCGGCGACAGGTGCCTCTGCTGCGGATACAGCGCCCATACCGCCGTATTCGGCGGTTGGTGCG‑CCTCCAGCAGCGACACCAGCGCACCACTGTTCAGgtgt                                                                                                 <  6:88967/119‑1 (MQ=255)
                                                                             ccaccaGCCGCCGTACCTTCGGCGACAGGTGCCTCTGCTGCGGATACAGCGCCCATACCGCCGTATTCGGCGGTTGGTGCG‑CCTCCAGCAGCGACACCAGCGCACCACTGTTCAGgtgt                                                                                                 >  5:88967/1‑119 (MQ=255)
                                                                                ccaGCCGCCGTACCTTCGGCGACAGGTGCCTCTGCTGCGGATACAGCGCCCATACCGCCGTATTCGGCGGTTGGTGCG‑CCTCCAGCAGCGACACCAGCGCACCACTGTTCAGGTGTTCCAGCACGTAGTAATCCGGCAACTGGCACAAc                                                                <  1:274406/149‑1 (MQ=255)
                                                                                     cgccgTACCTTCGGCGACAGGTGCCTCTGCTGCGGATACAGCGCCCATACCGCCGTATTCGGCGGTTGGTGCG‑CCTCCAGCAGCGACACCAGCGCACCACTGTTCAGGTGTTCCAGCACGTAGTAATCCGGCAACTGGCACAACCCGAt                                                           >  3:152056/1‑149 (MQ=255)
                                                                                     cgccgTACCTTCGGCGACAGGTGCCTCTGCTGCGGATACAGCGCCCATACCGCCGTATTCGGCGGTTGGTGCG‑CCTCCAGCAGCGACACCAGCGCACCACTGTTCAGGTGTTCCAGCACGTAGTAATCCGGCAACTGGCACAACCCGAt                                                           <  7:44245/149‑1 (MQ=255)
                                                                                      gccgTACCTTCGGCGACAGGTGCCTCTGCTGCGGATACAGCGCCCATACCGCCGTATTCGGCGGTTGGTGCG‑CCTCCAGCAGCGACACCAGCGCACCACTGTTCAGGTGTTCCAGCACGTAGTAATCCGGCAACTGGCACAACCCGAtt                                                          <  3:106534/149‑1 (MQ=255)
                                                                                          tACCTTCGGCGACAGGTGCCTCTGCTGCGGATACAGCGCCCATACCGCCGTATTCGGCGGTTGGTGCG‑CCTCCAGCAGCGACACCAGCGCACCACTGTTCAGGTGTTCCAGCACGTAGTAATCCGGCAACTGGCACAACCCGATACCtt                                                      <  5:247754/149‑1 (MQ=255)
                                                                                          aaggtacggcggCTGGTGCCTCTGCTGCGGATACAGCGCCCATACCGCCGTATTCGGCGGTTGGTGCG‑CCTCCAGCAGCGACACCAGCGCACCACTGTTCAGGTGTTCCAGCACGTAGTAATCCGGCAACTGGCACAACCCGATTCCtt                                                      <  2:181798/143‑1 (MQ=255)
                                                                                             cTTCGGCGACAGGTGCCTCTGCTGCGGATACAGCGCCCATACCGCCGTATTCGGCGGTTGGTGCG‑CCTCCAGCAGCGACACCAGCGCACCACTGTTCAGGTGTTCCAGCACGTAGTAATCCGGCAACTGGCACAACCCGATTCCTTGCa                                                   <  8:191571/149‑1 (MQ=255)
                                                                                             cTTCGGCGACAGGTGCCTCTGCTGCGGATACAGCGCCCATACCGCCGTATTCGGCGGTTGGTGCG‑CCTCCAGCAGCGACACCAGCGCACCACTGTTCAGGTGTTCCAGCACGTAGTAATCCGGCAACTGGCACAACCCGATTCCTTGCa                                                   <  7:257926/149‑1 (MQ=255)
                                                                                                             ctctGCTGCGGATACAGCGCCCATACCGCCGTATTCGGCGGTTGGTGCG‑CCTCCAGCAGCGACACCAGCGCACCACTGTTCAGGTGTTCCAGCACGTAGTAATCCGGCAACTgg                                                                      >  7:314913/1‑114 (MQ=255)
                                                                                                             ctctGCTGCGGATACAGCGCCCATACCGCCGTATTCGGCGGTTGGTGCG‑CCTCCAGCAGCGACACCAGCGCACCACTGTTCAGGTGTTCCAGCACGTAGTAATCCGGCAACTgg                                                                      <  8:314913/114‑1 (MQ=255)
                                                                                                                  ctgcGGATACAGCGCCCATACCGCCGTATTCGGCGGTTGGTGCG‑CCTCCAGCAGCGACACCAGCGCACCACTGTTCAGGTGTTCCAGCACgtagt                                                                                    >  8:64080/1‑95 (MQ=255)
                                                                                                                  ctgcGGATACAGCGCCCATACCGCCGTATTCGGCGGTTGGTGCG‑CCTCCAGCAGCGACACCAGCGCACCACTGTTCAGGTGTTCCAGCACgtagt                                                                                    <  7:64080/95‑1 (MQ=255)
                                                                                                                    gcGGATACAGCGCCCATACCGCCGTATTCGGCGGTTGGTGCG‑CCTCCAGCAGCGACACCAGCGCACCACTGTTCAGGTGTTACAGCACGTAGTAATCCGGCAACTGGCACAACCCGATTCCTTGCAGCGCCGCATCCAGCACTGCCTgg                            >  8:148254/1‑149 (MQ=255)
                                                                                                                         tACAGCGCCCATACCGCCGTATTGGTCGGTTGGTGCG‑CCTCCAGCAGCGACACCAGCTCACCACTGTTCAGGTGTTCCAGCACGTAGTAATCCGGCAACTGGCACAACCCGATTCCTTGCAGCGCCGCATGCAGCACTGCCTGGCCAc                        <  8:65946/148‑1 (MQ=255)
                                                                                                                            aGCGCCCATACCGCCGTATTCGGCGGTTGGTGCG‑CCTCCAGCAGCGACACCAGCGCACCACTGTTCAGGTGTTCCAGCACGTAGTAATCCGGCAACTGGCACAACCCGATTCCTTGCAGCGCCGCATCCAGCACTGCCTGGCCACTGtt                    >  7:271453/1‑149 (MQ=255)
                                                                                                                             gcgcCCATACCGCCGTATTCGGCGGTTGGTGCG‑CCTCCAGCAGCGACACCAGCGCACCACTGTTCAGGTGTTCCAGCACGTAGTAATCCGGCAACTGGCACAACCCGATTCCTTGCAGCGCCGCATCCAGCACTGCCTGGCCACTGTTg                   >  4:257181/1‑149 (MQ=255)
                                                                                                                               gcaccaaCCGCCGAATACGGCGGTTGGTGCG‑CCTCCAGCAGCGACACCAGCGCACCACTGTTCAGGTGGTCCAGCACGTAGTAATCCGGCAACTGGCACAACCCGATTCCTTGCAGCGCCGCATCCAGCACTGCCTGGCCACTGTTg                   >  6:199961/7‑147 (MQ=255)
                                                                                                                                     aCCGCCGTATTCGGCGGTTGGTGCG‑CCTCCAGCAGCGACACCAGCGCACCACTGTTCAGGTGTTCCAGCACGTAGTAATCCGGCAACTGGCACAACCCGATTCCTTGCAGCGCCGCATCCAGCACTGCCTGGCCACTGTTGCAgcgc             >  3:142531/1‑147 (MQ=255)
                                                                                                                                      ccgccgTATTCGGCGGTTGGTGCG‑CCTCCAGCAGCGACACCAGCGCACCACTGTTCAGGTGTTCCAGCACGTAGTAATCCGGCAACTGGCACAACCCGATTCCTTGCAGCGCCGCATCCAGCACTGCCTGGCCACTGTTGCAGCGCCAg          <  8:271453/149‑1 (MQ=255)
                                                                                                                                       cgccgTATTCGGCGGTTGGTGCG‑CCTCCAGCAGCGACACCAGCGCACCACTGTTCAGGTGTTCCAGCACGTAGTAATCCGGCAACTGGCACCTCCCGATTCCTTGCCGCGCCGCATCCAGCCCTGCCTGGCCAATGTTGCAGCGCCAGt         >  5:277205/1‑149 (MQ=255)
                                                                                                                                          cgTATTCGGCGGTTGGTGCG‑CCTCCAGCAGAGACACCAGCGCACCACTGTTCAGGTGTTCCAGCACGTAGTAATCCGGCAACTGGCACAACCCGATTCCTTGCAGCGCCGCATCCAGCACTGCCTGGCCACTGTTGCAGCGCCAGTTAc      <  6:70650/149‑1 (MQ=255)
                                                                                                                                            tATTCGGCGGTTGGTGCG‑CCTCCAGCAGCGACACCAGCGCACCACTGTTCAGGTGTTCCAGCACGTAGTAATCCg                                                                              >  4:45677/1‑75 (MQ=255)
                                                                                                                                            tATTCGGCGGTTGGTGCG‑CCTCCAGCAGCGACACCAGCGCACCACTGTTCAGGTGTTCCAGCACGTAGTAATCCg                                                                              <  3:45677/75‑1 (MQ=255)
                                                                                                                                              ttCGGCGGTTGGTGCG‑CCTCCAGCAGCGACACCAGCGCACCACTGTTCAGGTGTTCCAGCACGTAGTAATCCGGCAACTGGCACAACCCGATTCCTTGCAGCGCCGCATCCAGCACTGCCTGGCCACTGTTGCAGCGCCAGTTACCCTg  >  8:71911/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
AGAGGCCAGTACCAGCAGCACATCCCGTCAGCCGAAACGATACTCCGGCAACCCCGCCAACCCCTCCTTCAGGTAATCCACCAGCCGCCGTACCTTCGGCGACAGGTGCCTCTGCTGCGGATACAGCGCCCATACCGCCGTATTCGGCGGTTGGTGCG‑CCTCCAGCAGCGACACCAGCGCACCACTGTTCAGGTGTTCCAGCACGTAGTAATCCGGCAACTGGCACAACCCGATTCCTTGCAGCGCCGCATCCAGCACTGCCTGGCCACTGTTGCAGCGCCAGTTACCCTG  >  NC_002947/1811494‑1811784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑