Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,816,577 T→A 9.6% V150E (GTG→GAG)  surE → 5'‑nucleotidase SurE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,816,5770TA 9.6% 130.3 / 4.2 49V150E (GTG→GAG) surE5'‑nucleotidase SurE
Reads supporting (aligned to +/- strand):  ref base T (22/22);  new base A (3/2);  total (25/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATTCGGGAACGGTCGCTGCGGCGCTGGAAGGCCGCTTCCTCGGCGGTACCTCGCTGGCGTTCTCGCTGTTGTCGCGCCAGCCGGACAACCTGCCGACTGCGGCCTATATCGCCCGTCGTCTGGTCGAAGCACAGTCGCGTCTGGTGTTGCCGCCACGCACCGTACTCAACGTCAATATTCCCAACTTGCCGCTGGAGCACATCCGTGGCATCCAGCTCACCCGTCTCGGTCACCGGGCGCGGGCGGCGGCGCCCACCAAGGTGGTCAACCCGCGTGGCAAGGAAGGCTACTG  >  NC_002947/1816432‑1816724
                                                                                                                                                 |                                                                                                                                                   
tATTCGGGAACGGTCGCTGCGGCGCTGGAAGGCCGCTTCCTCGGCGGTACCTCGCTGGCGTTCTCGCTGTTGTCGCGCCAGCCGGACAACCTGCCGACTGCGGCCTATATCGCCCGTCGTCTGGTCGAAGCACAGTCGCGTCTGGTGtt                                                                                                                                                  <  5:318352/149‑1 (MQ=255)
           ggTCGCTGCGGCGCTGGAAGGCCGCTTCCTCGGCGGTACCTCGCTGGCGTTCTCGCTGTTGTCGCGCCAGCCGGACAACCTGCCGACTGCGGCCTATATCGCCCGTCGTCTGGTCGAAGCACAGTCGCGTCTGGTGTTGCCGCCACGCa                                                                                                                                       <  5:44547/149‑1 (MQ=255)
                     gcgcTGGAAGGCCGCTTCCTCGGCGGTACCTCGCTGGCGTTCTCGCTGTTGTCGCGCCAGCCGGACAACCTGCCGACTGCGGCCTATATCGCCCGTCGTCTGGTCGAAGCACAGTCGCGTCTGGTGTTGCCGCCACGCACCGTACTCaa                                                                                                                             >  8:134832/1‑149 (MQ=255)
                       gcTGGAAGGCCGCTTCCTCGGCGGTACCTCGCTGGCGTTCTCGCTGTTGTCGCGCCAGCCGGACAACCTGCCGACTGCGGCCTATATCGCCCGTCGTCTGGTCTAAGCACAGTCGCGTCTGGTGTTGCCGCCACGCACCGTa                                                                                                                                  <  7:154077/142‑1 (MQ=255)
                       gcTGGAAGGCAGCTTCCTCGGCGGTACCTCGCTGGCGTTCTCGCTGTTTTCGCTCCAGCAGGACAACCTGCCGACTGCCGCCTATATCGCCCGTCGTCTGTTCGAAGCACAGTCGCGTCTGGAGTTGCCGACACGAACCGTACACATCg                                                                                                                           >  8:154077/1‑149 (MQ=255)
                        cTGGAAGGCCGCTTCCTCGGCGGTACCTCGCTGGCGTTCTCGCTGTTGTCGCGCCAGCCGGACAACCTGCCGACTGCGGCCTATATCGCCCGTCGTCTGGTCGAAGCACAGTCGCGTCTGGAGTTGCCGCCACGCACCGTACTCAACGt                                                                                                                          >  5:137326/1‑149 (MQ=255)
                        cTGGAAGGCCGCTTCCTCGGCGGTACCTCGCTGGCGTTCTCGCTGTTGTCGCGCCAGCCGGACAACCTGCCGACTGCGGCCTATATCGCCCGTCGTCTGGTCGAAGCACAGTCGCGTCTGGAGTTGCCGCCACGCACCGTACTCAACGt                                                                                                                          >  5:132180/1‑149 (MQ=255)
                           gAAGGCCGCTTCCTCGGCGGTACCTCGCTGGCGTTCTCGCTGTTGTCGCGCCAGCCGGACAACCTGCCGACTGCGGCCTATATCGCCCGTCGTCTGGTCGAAGCACAGTCGCGTCTGGTGTTGCCGCCACGCACCGTACTCAACGTCa                                                                                                                        <  1:274919/148‑1 (MQ=255)
                                 cGCTTCCTCGGCGGTACCTCGCTGGCGTTCTCGCTGTTGTCGCGCCAGCCGGACAACCTGCCGACTGCGGCCTATATCGCCCGTCGTCTGGTCGAAGCACAGTCGCGTCTGGTGTTGCCGCCACGCACCGTACTCAACGTCAATATTcc                                                                                                                 <  4:174788/149‑1 (MQ=255)
                                  gCTTCCTCGGCGGTACCTCGCTGGCGTTCTCGCTGTTGTCGCGCCAGCCGGACAACCTGCCGACTGCGGCCTATATCGCCCGTCGTCTGGTCGAAGCACAGTCGCGTCTGGTGTTGCCGCCACGCACCGTACTCAACGTCAATATTccc                                                                                                                <  3:116983/149‑1 (MQ=255)
                                   cTTCCTCGGCGGTACCTCGCTGGCGTTCTCGCTGTTGTCGCGCCAGCCGGACAACCTGCCGACTGCGGCCTATATCGCCCGTCGTCTGGTCGAAGCACAGTCGCGTCTGGTGTTGCCGCCACGCACCGTACTCAACGTCAATATTccc                                                                                                                >  6:158320/1‑148 (MQ=255)
                                    ttCCTCGGCGGTACCTCGCTGGCGTTCTCGCTGTTGTCGCGCCAGCCGGACAACCTGCCGACTGCGGCCTATATCGCCCGTCGTCTGGTCGAAGCACAGTCGCGTCTGGTGTTGCCGCCACGCACCGTACTCAACGTCAATATTCCCaa                                                                                                              >  1:304733/1‑149 (MQ=255)
                                        tCGGCGGTACCTCGCTGGCGTTCTCGCTGTTGTCGCGCCAGCCGGACAACCTGCCGACTACGGCCTATATCGCCCGTCGTCTGGTCGAAGCACAGTCGCGTCTGGTGTTGCCGCCACGCACCGTACTCa                                                                                                                              <  3:62428/129‑1 (MQ=255)
                                        tCGGCGGTACCTCGCTGGCGTTCTCGCTGTTGTCGCGCCAGCCGGACAACCTGCCGACTACGGCCTATATCGCCCGTCGTCTGGTCGAAGCACAGTCGCGTCTGGTGTTGCCGCCACGCACCGTACTCa                                                                                                                              >  4:62428/1‑129 (MQ=255)
                                          ggcggTACCTCGCTGGCGTTCTCGCTGTTGTCGCGCCAGCCGGACAACCTGCCGACTGCGGCCTATATCGCCCGTCGTCTGGTCGAAGCACAGTCGCGTCTGGTGTTGCCGCCACGCACCGTACTCAACGTCAATATTCCCAACTTGc                                                                                                         <  4:136324/148‑1 (MQ=255)
                                                 ccTCGCTGGCGTTCTCGCTGTTGTCGCGCCAGCCGGACAACCTGCCGACTGCGGCCTATATCGCCCGTCGTCTGGTCGAAGCACAGTCGCGTCTGGTGTTGCCGCCACGCACCGTa                                                                                                                                  >  6:245555/1‑116 (MQ=255)
                                                 ccTCGCTGGCGTTCTCGCTGTTGTCGCGCCAGCCGGACAACCTGCCGACTGCGGCCTATATCGCCCGTCGTCTGGTCGAAGCACAGTCGCGTCTGGTGTTGCCGCCACGCACCGTa                                                                                                                                  <  5:245555/116‑1 (MQ=255)
                                                   tCGCTGGCGTTCTCGCTGTTGTCGCGCCAGCCGGACAACCTGCCGACTGCGGCCTATATCGCCCGTCGTCTGGTCGAAGCACAGTCGCGTCTGGTGTTGCCGCCCCGCACCGTACTCAACGTCAATATTCCCAACTTGCCGCTGGAGca                                                                                               >  8:242031/1‑149 (MQ=255)
                                                   tCGCTGGCGTTCTCGCTGTTGTCGCGCCAGCCGGACAACCTGCCGACTGCGGCCTATATCGCCCGTCGTCTGGTCGAAGCACAGTCGCGGCAGGTGTTGCCGCCAAGCACCGTACGCAACGTCAAAATTAACAAAATTCCGCTGGCGca                                                                                               >  5:209491/1‑149 (MQ=255)
                                                          cGTTCTCGCTGTTGTCGCGCCAGCCGGACAACCTGCCGACTGCGGCCTATATCGCCCGTCGTCTGGTCGAAGCACAGTCGCGTCTGGTGTTGCCGCCACGCACCGTACTCAACGTCAATATTCCCAACTTGCCGCTGGAGCACATCCGt                                                                                        <  4:9999/149‑1 (MQ=255)
                                                           gTTCTCGCTGTTGTCGCGCCAGCCGGACAACCTGCCGACTGCGGCCTATATCGCCCGTCGTCTGGTCGAAGCACAGTCGCGTCTGGTGTTGCCGCCACGCACCGTACTCAACGTCAATATTCCCAACTTGCCGCTGGAGCACATCCGTg                                                                                       <  3:34021/149‑1 (MQ=255)
                                                                  cTGTTGTCGCGCCAGCCGGACAACCTGCCGACTGCGGCCTATATCGCCCGTCGTCTGGTCGAAGCACAGTCGCGTGTGGTGTTGCCGCCCCGCACCGGACTCAACGGGAATTTTACCAACTTGCCGCTGGGGCACATCCGGGGCCTc                                                                                  >  1:339207/1‑147 (MQ=255)
                                                                          gcgcCAGCCGGACAACCTGCCGACTGCGGCCTATATCGCCCGTCGTCTGGTCGAAGCACAGTCGCGTCTGGTGTTGCCGCCACGCACCGTACTCAACGTCAATATTCCCAACTTGCCGCTGGAGCACATCCGTGGCATCCAGCTCAccc                                                                        >  7:45601/1‑149 (MQ=255)
                                                                                gCCGGACAACCTGCCGACTGCGGCCTATATCGCCCGTCGTCTGGTCGAAGCACAGTCGCGTCTGGTGTTGCCGCCACGCACCGTACTCAACGTCAATATTCCCAACTTGCCGCTGGAGCACATCCGTGGCATCCAGCTCAc                                                                          <  8:49266/141‑1 (MQ=255)
                                                                                gCCGGACAACCTGCCGACTGCGGCCTATATCGCCCGTCGTCTGGTCGAAGCACAGTCGCGTCTGGTGTTGCCGCCACGCACCGTACTCAACGTCAATATTCCCAACTTGCCGCTGGAGCACATCCGTGGCATCCAGCTCAc                                                                          >  7:49266/1‑141 (MQ=255)
                                                                                gCCGGACAACCTGCCGACTGCGGCCTATATCGCCCGTCGTCTGGTCGAAGCACAGTCGCGTCTGGTGTTGCCGCCACGCACCGTACTCAACGTCAATATTCCCAACTTGCCGCTGGAGCACATCCGTGGCATCCAGCTCACCCGTCTCg                                                                  <  4:10129/149‑1 (MQ=255)
                                                                                   ggACAACCTGCCGACTGCGGCCTATATCGCCCGTCGTCTGGTCGAAGCACAGTCGCGTCTGGTGTTGCCGCCACGCACCGTACTCAACGTCAATATTCCCAACTTGCCGCTGGAGCACATCCGTGGCATCCAGCTCACCCGTCTCGGt                                                                >  5:106526/1‑148 (MQ=255)
                                                                                            gCCGACTGCGGCCTATATCGCCCGTCGTCTGGTCGAAGCACAGTCGCGTCTGGTGTTGCCGCCACGCACCGTACTCAACGTCAATATTCCCAACTTGCCGCTGGAGCACATCCGTGGCATCCAGCTCACCCGTCTCGGTCACcgggcgc                                                      >  1:244320/1‑149 (MQ=255)
                                                                                                     ggCCTATATCGCCCGTCGTCTGGTCGAAGCACAGTCGCGTCTGGTGTTGCCGCCACGCACCGTACTCAACGTCAATATTCCCAACTTGCCGCAGGAGCACATCCGTGGCATCCAGCTCACCCGTCTCGGTCACCGGGCGCGggcggcgg                                             >  4:158531/1‑149 (MQ=255)
                                                                                                      gCCTATATCGCCCTTCGTCTGGTAGAAGCACAGTCGCGTCTGGTGGTGCCGCCACGCACCGTACTCAACGTCAATATTCCCAACTTGCCGCTGGAGCACATCCGTGGCATCCAGCTCACCCGTCTCGGTCACCGGGCGCGggcggcggc                                            <  8:265578/149‑1 (MQ=255)
                                                                                                       ccTATATCGCCCGTCGTCTGGTCGAAGCACAGTCGCGTCTGGTGTTGCCGCCCCGCACCGTACTCAACGTCAATATTCCCAACTTGCCGCTGGAGCACATCCGTGGCATCCAGCTCACCCGTCTCGGTCACCGGGCGCGGgcggcggcg                                           >  7:211742/1‑149 (MQ=255)
                                                                                                           tatCGCCCGTCGTCTGGTCGAAGCACAGTCGCGTCTGGTGTTGCCGCCACGCACCGTACTCAACGTCAATATTCCCAACTTGCCGCTGGAGCACATCCGTGGCATCCAGCTCACCCGTCTCGGTCACCGGGCGCCGCCGCCCGCGcccg                                       <  4:336139/149‑2 (MQ=255)
                                                                                                                  cgtcgtCTGGTCGAAGCACAGTCGCGTCTGGTGTTGCCGCCACGCACCGTACTCAACGTCAATATTCCCAACTTGCCGCTGGAGCACATCCGTGGCATCCAGCTCACCCGTCTCGGTCACCGGGCGCGGGCGGCGGCGCCCACCAAggt                                <  2:244320/149‑1 (MQ=255)
                                                                                                                   gtcgtcTGGTCGAAGCACAGTCGCGTCTGGTGTTGCCGCCACGCACCGTACTCAACGTCAATATTCCCAACTTGCCGCTGGAGCACATCCGTGGCATCCAGCTCACCCGTCTCGGTCACCGGGCGCGGGCGGCGGCGCCCACCAAggtg                               <  3:158531/149‑1 (MQ=255)
                                                                                                                   gtcgtcTGGTCGAAGCACAGTCGCGTCTGGTGTTGCCGCCACGCACCGTACTCAACGTCAATATTCCCAACTTGCCGCTGGAGCACATCCGTGGCATCCAGCTCACCCGTCTCGGTCACCGGGCGCGGGCGGCGGCGCCCACCAAggtg                               <  5:158320/149‑1 (MQ=255)
                                                                                                                    tcgtcTGGTCGAAGCACAGTCGCGTCTGGTGTTGCCGCCCCGCCCCGTACTGAACGTCAATATTCCGAACTTGCCGCTGGAGCACATGCGTGGCATCGAGCTCCCCCGGCGCGGTCACCGGGGGCGGGCGGCGGGGCCCACCAAg                                  >  6:33907/1‑145 (MQ=255)
                                                                                                                    tcgtcTGGTCGAAGCACAGTCGCGTCTGGTGTTGCCGCCACGCACCGTACTCAACGTCAATATTCCTAACTTGCCGCTGGAGCACATCCGTGGCATCCAGCTCACCCGTCTCGGTCACCGGGCGCGGGCGGCGGCGCCCACCAAg                                  <  5:33907/145‑1 (MQ=255)
                                                                                                                      gtcTGGTCGAAGCACAGTCGCGTCTGGTGTTGCCGCCACGCACCGTACTCAACGTCAATATTCCCAACTTGCCGCTGGAGCACATCCGTGGCATCCAGCTCACCCGTCTCGGTCACCGGGCGCGGGCGGCGGCGCCCACCAAggtggt                             <  8:45601/148‑1 (MQ=255)
                                                                                                                        cTGGTCGAAGCACAGTCGCGTCTGGTGTTGCCGCCACGCACCGTACTCAACGTCAATATTCCCAACTTGCCGCTGGAGCACATCCGTGGCA‑CCAGCTCACCCGTCTCGGTCACCGGGCGCGGGCGGCGGCGCCCACCAAGGTGGTCAAc                         <  2:304733/149‑1 (MQ=255)
                                                                                                                           gTCGAAGCACAGTCGCGTCTGGTGTTGCCGCCACGCACCGTACTCAACGTCAATATTCCCAACTTGCCGCTGGAGCACATCCGTGGCATCCAGCTCACCCGTCTCGGTCACCGGGCGCGGGCGGCGGCGCCCACCAAGGTGGTCAAccc                       >  7:157094/1‑149 (MQ=255)
                                                                                                                           gTCGAAGCACAGTCGCGTCTGGAGTTGCCGCCACGCACCGTACTCAACGTCAATATTCCCAACTTGCCGCTGGAGCACATCCGTGGCATCCAGCTCACCCGTCTCGGTCACCGGGCGCGGGCGGCGGCGCCCACCAAggtgggcgccg                        <  6:137326/148‑7 (MQ=255)
                                                                                                                           gTCGAAGCACAGTCGCGTCTGGAGTTGCCGCCACGCACCGTACTCAACGTCAATATTCCCAACTTGCCGCTGGAGCACATCCGTGGCATCCAGCTCACCCGTCTCGGTCACCGGGCGCGGGCGGCGGCGCCCACCAAggtgggcgccg                        <  6:132180/148‑7 (MQ=255)
                                                                                                                           gTCGAACCACAGTCGCGTCTGGTGTTGCCGCCACGCACCGTACTCAACGTCAATATTCCCAACTTGCCGCTGGAGCACATCCGTGGCATCCAGCTCACCCGTCTCGGTCACCGGGCGCGGGCGGCGGCGCCCACCAAGGTGGTCAAccc                       >  2:62629/1‑149 (MQ=255)
                                                                                                                            tCGAAGCACAGTCGCGTCTGGTGTTGCCGCCACGCACCGTACTCAACGTCAATATTCCCAACTTGCCGCTGGAGCACATCCGTGGCATCCAGCTCACCCGTCTCGGTCACCGGGCGCGGGCGGCGGCGCCCACCAAGGTGGTCAACCcg                      >  2:176754/1‑149 (MQ=255)
                                                                                                                               aaGCACAGTCGCGTCTGGTGTTGCCGCCACGCACCGTACTCAACGTCAATATTCCCAACTTGCCGCTGGAGCACATCCGTGGCATCCAGCTCACCCGTCTCGGTCACCGGGCGCGGGCGGCGGCGCCCACCAAGGTGGTCAACCCGCGt                   >  7:171623/1‑149 (MQ=255)
                                                                                                                                   acaGTCGCGTCTGGTGTTGCCGCCACGCACCGTACTCAACGTCAATATTCCCAACTTGCCGCTGGAGCACATCCGTGGCATCCAGCTCACCCGTCTCGGTCACCGGGCGCGGGCGGCGGCGCCCACCAAGGTGGTCAACCCGCGTGGc                >  1:192071/1‑148 (MQ=255)
                                                                                                                                   acaGTCGCGTCTGGTGTTGCCGCCACGCACCGTACTCAACGGCAATATTCCCAACTTGCCGCTGGAGCACATCCGTGGCATCCAGCTCCCCCGTCTCGGTCACCGGGCGCGGGCGGCGGCGCCCACCAAGGTGGTCAACCCGCGTGGc                >  6:269218/1‑148 (MQ=255)
                                                                                                                                         gcgTCTGGTGTTGCCGCCACGCACCGTACTCAACGTCAATATTCCCAACTTGCCGCTGGAGCACATCCGTGGCATCCAGCTCACCCGTCTCGGTCACCGGGCGCGGGCGTCGGCGCCCACCAAGGTGGTCAACCCGCGTGGCaaggaa          <  6:209491/148‑1 (MQ=255)
                                                                                                                                           gTCTGGTGTTGCCGCCACGCACCGTACTCAACGTCAATATTCCAAAATTCCCGCTGATGCACATCCTTGCCAACCGCAACCCCCGTCACGGACACCGGCCCCGGGCGCCGCCCCCACCCAAGGTGGACAACCCGCGTGCcaagcaagc        >  6:145435/1‑147 (MQ=255)
                                                                                                                                                gtgtTGCCGCCACGCACCGTACTCAACGTCAATATTCCCAACTTGCCGCTGGAGCACATCCGTGGCATCCAGCTCACCCGTCTCGGTCACCGGGCGCGGGCGGCGGCGCCCACCAAGGTGGTCAACCCGCGTGGCAAGGAAGGCTACt   <  1:176754/148‑1 (MQ=255)
                                                                                                                                                gtgtTGCCGCCACGCACCGTACTCAACGTCAATATTCCCAACTTGCCGCTGGAGCACATCCGTGGCATCCAGCTCACCCGTCTCGGTCACCGGGCGCGGGCGGCGGCGCCCACCAAGGTGGTCAACCCGCGTGGCAAGGAAGGCTACTg  <  7:134832/149‑1 (MQ=255)
                                                                                                                                                 tgtTGCCGCCACGCACCGTACTCAACGTCAATATTCCCAACTTGCCGCTGGAGCACATCCGTGGCATCCAGCTCACCCGTCTCGGTCACCGGGCGCGGGCGGCGGCGCCCACCAAGGTGGTCAACCCGCGTGGCAAGGAAGGCTACTg  >  2:157560/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
TATTCGGGAACGGTCGCTGCGGCGCTGGAAGGCCGCTTCCTCGGCGGTACCTCGCTGGCGTTCTCGCTGTTGTCGCGCCAGCCGGACAACCTGCCGACTGCGGCCTATATCGCCCGTCGTCTGGTCGAAGCACAGTCGCGTCTGGTGTTGCCGCCACGCACCGTACTCAACGTCAATATTCCCAACTTGCCGCTGGAGCACATCCGTGGCATCCAGCTCACCCGTCTCGGTCACCGGGCGCGGGCGGCGGCGCCCACCAAGGTGGTCAACCCGCGTGGCAAGGAAGGCTACTG  >  NC_002947/1816432‑1816724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑