Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,887,296 G→A 7.4% L993L (CTG→TTG)  PP_1695 ← sodium‑solute symporter/two‑component system sensor histidine kinase/response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,887,2960GA 7.4% 194.5 / 4.6 67L993L (CTG→TTG) PP_1695sodium‑solute symporter/two‑component system sensor histidine kinase/response regulator
Reads supporting (aligned to +/- strand):  ref base G (33/29);  new base A (2/3);  total (35/32)
Fisher's exact test for biased strand distribution p-value = 6.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.23e-01

ATAGCACTTGCAGCCCGGCCAGTGGCTGGCCGCCCTGTTGTTCCACGGGCGTGCTTGGCGCCGCGGCGGCCTGGCGTGCAATCGGTACGCTGACGCGGAACACCGTGCCCTTGCCTGGCCATGAGCGCACTTCCAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTTCTCGGCACGGGTCTGGTGGCTGTCCAGGCGCTTGAACTCCTGAAAGATCACTTGCAGCTTGTCGTCAGCAATGCCCGGGCCACGGTCCCAGACCTCCAGC  >  NC_002947/1887149‑1887444
                                                                                                                                                   |                                                                                                                                                    
aTAGCACTTGCAGCCCGGCCAGTGGCTGGCCGCCCTGTTGTTCCACGGGCGTGCTTGGCGCCGCGGCGGCCTGGCGTGCAATCGGTACGCTGACGCGGAACACCGTGCCCTTGCCTGGCCATGAGCGCACTTCCAGCGGATGCCCCAg                                                                                                                                                      >  5:220923/1‑148 (MQ=255)
      cTTGCAGCCCGGCCAGTGGCTGGCCGCCCTGTTGTTCCACGGGCGTGCTTGGCGCCGCGGCGGCCTGGCGTGCAATCGGTACGCTGACGCGGAACACCGTGCCCTTGCCTGGCCATGAGCGCACTTCCAGCGGATGCCCCAGCACCCGa                                                                                                                                               <  7:69442/149‑1 (MQ=255)
          cAGCCCGGCCAGTGGCTGGCCGCCCTGTTGTTCCACGGGCGTGCTTGGCGCCGCGGCGGCCTGGCGTGCAATCGGTACGCTGACGCGGAACACCGTGCCCTTGCCTGGCCATGAGCGCACTTCCAGCGGATGCCCCAGCACCCGacac                                                                                                                                            <  7:65662/148‑1 (MQ=255)
          cAGCCCGGCCAGTGGCTGGCCGCCCTGTTGTTCCACGGGCGTGCTTGGCGCCGCGGCGGCCTGGCGTGCAATCGGTACGCTGACGCGGAACACCGTGCCCTTGCCTGGCCATGAGCGCACTTCCAGCGGATGCCCCAGCACCCGaca                                                                                                                                             >  3:168495/1‑147 (MQ=255)
           aGCCCGGCCAGTGGCTGGCCGCCCTGTTGTTCCACGGGCGTGCTTGGCGCCGCGGCGGCCTGGCGTGCAATCGGTACGCTGACGCGGAACACCGTGCCCTTGCCTGGCCATGAGCGCACTTCCAGCGGATGCCCCAGCACCCGaca                                                                                                                                             >  7:100877/1‑146 (MQ=255)
               cGGCCAGTGGCTGGCCGCCCTGTTGTTCCACGGGCGTGCTTGGCGCCGCGGCGGCCTGGCGTGCAATCGGTACGCTGACGCGGAACACCGTGCCCTTGCCTGGCCATGAGCGCACTTCCAGCGGATGCCCCAGCACCCGACACAGACCg                                                                                                                                      >  4:261335/1‑149 (MQ=255)
                      tggctggcCGCCCTGTTGTTCCACGGGCGTGCTTGGCGCCGCGGCGGCCTGGCGTGCAATCGGTACGCTGACGCGGAACACCGTGCCCTTGCCTGGCCATGAGCGCACTTCCAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCaa                                                                                                                               <  5:40022/149‑1 (MQ=255)
                        gctggcCGCCCTGTTGTTCCACGGGCGTGCTTGGCGCCGCGGCGGCCTGGCGTGCAATCGGTACGCTGACGCGGAACACCGTGCCCTTGCCTGGCCATGAGCGCACTTCCAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATg                                                                                                                             <  8:100877/149‑1 (MQ=255)
                         ctggcCGCCCTGTTGTTCCACGGGCGTGCTTGGCGCCGCGGCGGCCTGGCGTGCAATCGGTACGCTGACGCGGAACACCGTGCCCTTGCCTGGCCATGAGCGCACTTCCAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATgg                                                                                                                            <  1:35133/149‑1 (MQ=255)
                               gccCTGTTGTTCCACGGGCGTGCTTGGCGCCGCGGCGGCCTGGCGTGCAATCGGTACGCTGACGCGGAACACCGTGCCCTTGCCTGGCCATGAGCGCACTTCCAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGc                                                                                                                      <  8:147354/149‑1 (MQ=255)
                                    gttgttCCACGGGCGTGCTTGGCGCCGCGGCGGCCTGGCGTGCAATCGGTACGCTGACGCGGAACACCGTGCCCTTGCCTGGCCATGAGCGCACTTCCAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAg                                                                                                                 >  1:194234/1‑149 (MQ=255)
                                        ttCCACGGGCGTGCTTGGCGCCGCGGCGGCCTGGCGTGCAATCGGTACGCTGACGCGGAACACCGTGCCCTTGCCTGGCCATGAGCGCACTTCCAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGcc                                                                                                               >  5:348756/1‑147 (MQ=255)
                                        ttCCACGGGCGTGCTTGGCGCCGCGGCGGCCTGGCGTGCAATCGGTACGCTGACGCGGAACACCGTGCCCTTGCCTGGCCATGAGCGCACTTCCAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCt                                                                                                              <  6:327706/148‑1 (MQ=255)
                                        ttCCACGGGCGTGCTTGGCGCCGCGGCGGCCTGGCGTGCAATCGGTACGCTGACGCGGAACACCGTGCCCTTGCCTGGCCATGAGCGCACTTCCAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTa                                                                                                             <  3:261335/149‑1 (MQ=255)
                                           cACGGGCGTGCTTGGCGCCGCGGCGGCCTGGCGTGCAATCGGTACGCTGACGCGGAACACCGTGCCCTTGCCTGGCCATGAGCGCACTTCCAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGcc                                                                                                          >  4:316925/1‑149 (MQ=255)
                                              gggCGTGCTTGGCGCCGCGGCGGCCTGGCGTGCAATCGGTACGCTGACGCGGAACACCGTGCCCTTGCCTGGCCATGAGCGCACTTCCAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCt                                                                                                       >  4:26866/1‑149 (MQ=255)
                                                  gTGCTTGGCGCCGCGGCGGCCTGGCGTGCAATCGGTACGCTGACGCGGAACACCGTGCCCTTGCCTGGCCATGAGCGCACTTCCAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTtctc                                                                                                   <  3:316925/149‑1 (MQ=255)
                                                    gCTTGGCGCCGCGGCGGCCTGGCGTGCAATCGGTACGCTGACGCGGAACACCGTGCCCTTGCCTGGCCATGAGCGCACTTCCAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTTCTCgg                                                                                                 >  1:69233/1‑149 (MQ=255)
                                                    gCTTGGCGCCGCGGCGGCCTGGCGTGCAATCGGTACGCTGACGCGGAACACCGTGCCCTTGCCTGGCCATGAGCGCACTTCCAGCGGATGCCCCAACACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTTCTCgg                                                                                                 >  4:161693/1‑149 (MQ=255)
                                                      ttGGCGCCGCGGCGGCCTGGCGTGCAATCGGTACGCTGACGCGGCACACCGTGCCCTTGCCTGGCCATGAGCGCACTTCCAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTTCTCGGCa                                                                                               >  5:263197/1‑149 (MQ=255)
                                                                ggcggcCTGGCGTGCAATCGGTACGCTGACGCGGAACACCGTGCCCTTGCCTGGCCATGAGCGCACTTCCAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTTCTCGGCACGGGTCtggt                                                                                     <  3:26866/149‑1 (MQ=255)
                                                                   ggcCTGGCGTGCAATCGGTACGCTGACGCGGAACACCGTGCCCTTGCCTGGCCATGAGCGCACTTCCAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTTCTCGGCACGGGTCTGGTGGc                                                                                  <  4:168495/149‑1 (MQ=255)
                                                                      cTGGCGTGCAATCGGTACGCTGACGCGGAACACCGTGCCCTTGCCTGGCCATGAGCGCACTTCCAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTTCTCGGCACGGGTCTGGTGGCTGt                                                                               >  5:97636/1‑149 (MQ=255)
                                                                      cTGGCGTGCAATCGGTACGCTGACGCGGAACACCGTGCCCTTGCCTGGCCATGAGCGCACTTCCAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTTCTCGGCACGGGTCTGGTGGCTGt                                                                               >  4:121416/1‑149 (MQ=255)
                                                                            tGCAATCGGTACGCTGACGCGGAACACCGTGCCCTTGCCTGGCCATGAGCGCACTTCCAGCGGATGCCCCAACACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTTCTCGGCACGGGTCTGGTGGCTGTCCAGgc                                                                         <  5:229097/149‑1 (MQ=255)
                                                                               aaTCGGTACGCTGACGCGGAACACCGTGCCCTTGCCTGGCCATGAGCGCACTTCCAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTTCTCGGCACGGGTCtg                                                                                       >  4:329201/1‑132 (MQ=255)
                                                                                        gCTGACGCGGAACACCGTGCCCTTGCCTGGCCATGAGCGCACTTCCAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTTCTCGGCACGGGTCTGGTGGCTGTCCAGGCGCTTGAACTCCt                                                             >  8:101350/1‑149 (MQ=255)
                                                                                          tGACGCGGAACACCGTGCCCTTGCCTGGCCATGAGCGCACTTCCAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTTCTCGGCACGGGTCTGGTGGCTGTCCAGGCGCTTg                                                                    >  7:242853/1‑140 (MQ=255)
                                                                                          tGACGCGGAACACCGTGCCCTTGCCTGGCCATGAGCGCACTTCCAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTTCTCGGCACGGGTCTGGTGGCTGTCCAGGCGCTTg                                                                    <  8:242853/140‑1 (MQ=255)
                                                                                           gACGCGGAACACCGTGCCCTTGCCTGGCCATGAGCGCACTTCCAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTTCTCGGCACGGGTCTGGTGGCTGTCCAGGCGCTTGAACTCCTGa                                                           >  1:151441/1‑148 (MQ=255)
                                                                                            aCGCGGAACACCGTGCCCTTGCCTGGCCATGAGCGCACTTCCAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTtctc                                                                                                   <  1:192842/107‑1 (MQ=255)
                                                                                            aCGCGGAACACCGTGCCCTTGCCTGGCCATGAGCGCACTTCCAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTtctc                                                                                                   >  2:192842/1‑107 (MQ=255)
                                                                                            aCGCGGAACACCGTGCCCTTGCCTGGCCATGAGCGCACTTCCAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTTCTCGGCACGGGTCTGGTGGCTGTCCAGGCGCTTGAACTCCTGa                                                           >  8:293745/1‑147 (MQ=255)
                                                                                            aCGCGGAACACCGTGCCCTTGCCTGGCCATGAGCGCACTTCCAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTTCTCGGCACGGGTCTGGTGGCTGTCCAGGCGCTTGAACTCCTGa                                                           <  7:101350/147‑1 (MQ=255)
                                                                                             cgcgGAACACCGTGCCCTTGCCTGGCCATGAGCGCACTTCCAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTTCTCGGCACGGGTCTGGTGGCTGTCCAGGCGCTTGAACTCCTGaaa                                                         <  3:116832/148‑1 (MQ=255)
                                                                                              gcgGAACACCGTGCCCTTGCCTGGCCATGAGCGCACTTCCAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTTCTCGGCACGGGTCTGGTGGCTGTCCAGGCGCTTGAACTCCTGa                                                           >  5:203769/1‑145 (MQ=255)
                                                                                                ggAACACCGTGCCCTTGCCTGGCCATGAGCGCACTTCCAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTTCTCGGCACGGGTCTGGTGGCTGTCCAGGCGCTTGAACTCCTGAAAGAt                                                      >  3:75876/1‑148 (MQ=255)
                                                                                                 gAACACCGTGCCCTTGCCTGGCCATGAGCGCACTTCCAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTTCTCGGCACGGGTCTGGTGGCTGTCCAGGCGCTTGAACTCCTGAAAGATCa                                                    >  5:309706/1‑149 (MQ=255)
                                                                                                  aaCACCGTGCCCTTGCCTGGCCATGAGCGCACTTCCAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTTCTCGGCACGGGTCTGGTGGCTGTCCAGGCGCTTGAACTCCTGAAAGATCAc                                                   <  6:348756/149‑1 (MQ=255)
                                                                                                       cGTGCCCTTGCCTGGCCATGAGCGCACTTCCAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTTCTCGGCACGGGTCTGGTGGCTGTCCAGGCGCTTGAACTCCTGAAAGATCACt                                                  <  5:62761/145‑1 (MQ=255)
                                                                                                       cGTGCCCTTGCCTGGCCATGAGCGCACTTCCAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTTCTCGGCACGGGTCTGGTGGCTGTCCAGGCGCTTGAACTCCTGAAAGATCACt                                                  >  6:62761/1‑145 (MQ=255)
                                                                                                       cGTGCCCTTGCCTGGCCATGAGCGCACTTCCAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTTCTCGGCACGGGTCTGGTGGCTGTCCAGGCGCTTGAACTCCTGAAAGATCACTTg                                                >  4:177708/1‑147 (MQ=255)
                                                                                                       cGTGCCCTTGCCTGGCCATGAGCGCACTTCCAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTTCTCGGCACGGGTCTGGTGGCTGTCCAGGCGCTTGAACTCCTGAAAGATCACTTGCa                                              <  2:194234/149‑1 (MQ=255)
                                                                                                       cGTGCCCTTGCCTGGCCATGAGCGCACTTCCAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTTCTCGGCACGGGTCTGGTGGCTGTCCAGGCGCTTGAACTCCTGAAAGATCACTTGCa                                              <  6:220923/149‑1 (MQ=255)
                                                                                                         tGCCCTTGCCTGGCCATGAGCGCACTTCCAGCGGATGCCCCAACACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTTCTCGGCACGGGTCTGGTGGCTGTCCAGGCGCTTGAACTCCTGAAAGATCACTTGCAGc                                            <  3:161693/149‑1 (MQ=255)
                                                                                                           cccTTGCCTGGCCATGAGCGCACTTCCAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTTCTCCGCACGGGTCTGGTGGCTGTCCAGGCGCTTGAACTCCTGAAAGATCACTTGCAGCtt                                          >  4:153799/1‑149 (MQ=255)
                                                                                                             cTTGCCTGGCCATGAGCGCACTTCCAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTTCTCGGCACGGGTCTGGTGGCTGTCCAGGCGCTTGAACTCCTGAAAGATCACTTGCAGCTTgt                                        >  2:53139/1‑149 (MQ=255)
                                                                                                              ttGCCTGGCCATGAGCGCACTTCCAGCGGATGCCCCAACACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTTCTCGGCACGGGTCTGGTGGCTGTCCAGGCGCTTGAACTCCTGAAAGATCACTTGCAGCTTgtc                                       >  3:67486/1‑149 (MQ=255)
                                                                                                                gCCTGGCCATGAGCGCACTTCCAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTTCTCGGCACGGGTCTGGTGGCTGTCCAGGCGCTTGAACTCCTGAAAGATCACTTGCAGCTTgtc                                       <  3:177708/147‑1 (MQ=255)
                                                                                                                 ccTGGCCATGAGCGCACTTCCAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTTCTCGGCACGGGTCTGGTGGCTGTCCAGGCGCTTGAACTCCTGAAAGATCACTTGCAGCTTgtcgt                                     <  6:97636/148‑1 (MQ=255)
                                                                                                                  cTGGCCATGAGCGCACTTCCAGCGGATGCCCCAACACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTTCTCGGCACGGGTCTGGTGGCTGTCCAGGCGCTTGAACTCCTGAAAGATCACTTGCAGCTTGTCGTCa                                   <  4:67486/149‑1 (MQ=255)
                                                                                                                   tGGCCATGAGCGCACTTCCAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTTCTCGGCACGGGTCTGGTGGCTGTCCAGGCGCTTGAACTCCTGAAAGATCACTTGCAGCTTGTCGTCAg                                  >  8:87115/1‑149 (MQ=255)
                                                                                                                    ggCCATGAGCGCACTTCCAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTTCTCGGCACGGGTCTGGTGGCTGTCCAGGCGCTTGAACTCCTg                                                            <  3:13063/122‑1 (MQ=255)
                                                                                                                    ggCCATGAGCGCACTTCCAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTTCTCGGCACGGGTCTGGTGGCTGTCCAGGCGCTTGAACTCCTg                                                            >  4:13063/1‑122 (MQ=255)
                                                                                                                     gCCATGAGCGCACTTCCAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTTCTCGGCACGGGTCTGGTGGCTGTCCAGGCGCTTGAACTCCTGAAAGATCACTTGCAGCTTGTCGTCAg                                  <  3:153799/147‑1 (MQ=255)
                                                                                                                        aTGAGCGCACTTCCAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTTCTCGGCACGGGTCTGGTGGCTGTCCAGGCGCTTGAACTCCTGAAAGATCACTTGCAGCTTGTCGTCAGCAATg                             <  1:53139/149‑1 (MQ=255)
                                                                                                                             cgcACTTCCAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTTCTCGGCACGGGTCTGGTGGCTGTCCAGGCGCTTGAACTCCTGAAAGATCACTTGCAGCTTGTCGTCAGCAATGCCCgg                        >  6:56303/1‑149 (MQ=255)
                                                                                                                                  ttCCAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTTCTCGGCACGGGTCTGGTGGCTGTCCAGGCGCTTGAACTCCTGAAAGATCACTTGCAGCTTGTCGTCAGCAATGCCCGGGCCAc                   >  5:173046/1‑149 (MQ=255)
                                                                                                                                  ttCCAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTTCTCGGCACGGGTCTGGTGGCTGTCCAGGCGCTTGAACTCCTGAAAGATCACTTGCAGCTTGTCGTCAGCAATGCCCGGGCCAc                   >  8:70359/1‑149 (MQ=255)
                                                                                                                                   tCCAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTTCTCGGCACGGGTCTGGTGGCTGTCCAGGCGCTTGAACTCCTGAAAGATCACTTGCAGCTTGTCGTCAGCAATGCCCGGGCCACg                  >  2:93727/1‑149 (MQ=255)
                                                                                                                                    ccAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTTCTCGGCACGGGTCTGGTGGCTGTCCAGGCGCTTGAACTCCTGAAAGATCACTTGCAGCTTGTCGTCAGCAATGCCCGGGCCACgg                 >  4:106198/1‑149 (MQ=255)
                                                                                                                                     cAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTTCTCGGCACGGGTCTGGTGGCTGTCCAGGCGCTTGAACTCCTGAAAGATCACTTGCAGCTTGTCGTCAGCAATGCCCGGGCCACGGt                >  5:227855/1‑149 (MQ=255)
                                                                                                                                     cAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTTCTCGGCACGGGTCTGGTGGCTGTCCAGGCGCTTGAACTCCTGAAAGATCACTTGCAGCTTGTCGTCAGCAATGCCCGGGCCACGGt                <  7:293745/149‑1 (MQ=255)
                                                                                                                                      aGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTTCTCGGCACGGGTCTGGTGGCTGTCCAGGCGCTTGAACTCCTGAAAGATCACTTGCAGCTTGTCGTCAGCAATGCCCGGGCCACGGTc               <  7:87115/149‑1 (MQ=255)
                                                                                                                                        cGGATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTTCTCGGCACGGGTCTGGTGGCTGTCCAGGCGCTTGAACTCCTGAAAGATCACTTGCAGCTTGTCGTCAGCAATGCCCGGGCCACGGTccc             <  6:203769/149‑1 (MQ=255)
                                                                                                                                          gATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTTCTCGGCACGGGTCTGGTGGCTGTCCAGGCGCTTGAACTCCTGAAAGATCACTTGCAGCTTGTCGTCAGCAATGCCCGGGCCACGGTCCCAg           <  3:106198/149‑1 (MQ=255)
                                                                                                                                               cccAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTTCTCGGCACGGGTCTGGTGGCTGTCCAGGCGCTTGAACTCCTGAAAGATCACTTGCAGCTTGTCGTCAGCAATGCCCGGGCCACGGTCCCAGACCTc      >  1:257413/1‑149 (MQ=255)
                                                                                                                                                ccAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTTCTCGGCACGGGTCTGGTGGCTGTCCAGGCGCTTGAACTCCTGAAAGATCACTTGCAGCTTGTCGTCAGCAATTCCCGGGCCACGGTCCCAGACCTcc     <  4:75876/149‑1 (MQ=255)
                                                                                                                                                   gCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTTCTCGGCACGGGTCTGGTGGCTGTCCAGGCGCTTGAACTCCTGAAAGATCACTTGCAGCTTGTCGTCAGCAATGCCCGGGCCACGGTCCCAGACCTCCAGc  <  5:56303/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
ATAGCACTTGCAGCCCGGCCAGTGGCTGGCCGCCCTGTTGTTCCACGGGCGTGCTTGGCGCCGCGGCGGCCTGGCGTGCAATCGGTACGCTGACGCGGAACACCGTGCCCTTGCCTGGCCATGAGCGCACTTCCAGCGGATGCCCCAGCACCCGACACAGACCGTCTGCAATGGCCAGGCCCAAGCCTAGCCCCTTCTCGGCACGGGTCTGGTGGCTGTCCAGGCGCTTGAACTCCTGAAAGATCACTTGCAGCTTGTCGTCAGCAATGCCCGGGCCACGGTCCCAGACCTCCAGC  >  NC_002947/1887149‑1887444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑