Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,905,646 A→T 9.5% H634L (CAC→CTC)  nirB → nitrite reductase large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,905,6460AT 9.5% 132.0 / 3.7 52H634L (CAC→CTC) nirBnitrite reductase large subunit
Reads supporting (aligned to +/- strand):  ref base A (26/21);  new base T (3/2);  total (29/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCATCGGCCAGGTGGCCAAGAAATACGACCTGTACACCAAGATCACCGGTGGCCAGCGCATCGACCTGTTCGGCGCCCAGTTGCACGAGCTGCCGCTGATCTGGGGCGAACTGATCGAGGCCGGTTTCGAAACCGGCCACGCCTACGGCAAGTCGACCCGCACGGTAAAGTCGTGCGTGGGCAGCACCTGGTGCCGTTACGGCGTGCAGGACAGTGTCGCCATGGCCCTGCGCCTGGAAGATCGCTACAAGGGCCTGCGCAGCCCCCACAAGCTCAAGTTCGCGG  >  NC_002947/1905508‑1905792
                                                                                                                                          |                                                                                                                                                  
tcatcgGCCAGGTGGCCAAGAAATACGACCTGTACACCAAGATCACCGGTGGCCAGCGCATCGACCTGTTCGGCGCCCAGTTGCACGAGCTGCCGCTGATCTGGGGCGAACTGATCGAGGCCGGTTTCGAAACCGGCCACGCCTACgg                                                                                                                                           >  3:243136/1‑148 (MQ=255)
 aagcgGCCAGCTCGCCAAGAAATACGACCTGTCAAACAAGATACCAGTTGGCCAGCGAATCGACATGTTCGGCCCCCAGTTTCACTCGCTGCCGCGGATCTGGGGCTAACTGATCGAGGCCGGTTTCGAAACCGGCCACGCCTACGGc                                                                                                                                          <  8:303814/145‑1 (MQ=255)
     ggcctcgTGCCCAAGAAATCCGCCCTTAACACCACCATCACCGGTGGCCAGCGCATCGACATGTCGGGCCCCCATTCGCACGCGCTCCCGGTGATCGGGGGTGAACTGAGCGCGGCCGGTTGCGAACCGGGCCACGCCTACGGCAAGTc                                                                                                                                     <  8:113195/143‑1 (MQ=255)
        ctgttgcccAATAAATACGACCTTTACACAACCAGCCCCGGGGCCCAGCGCCCCGACGTGTTCGGCGCCCTGTTCCACGAGCTGCCGCTGATGTGGGGCTAAGTTATCGCGGCGGTTTTCTAAACCGGCCACGCCTACGGCAAGTCGAc                                                                                                                                  <  4:333798/142‑1 (MQ=255)
         aGGTGGCCAAGAAATACGCCCTGTACACCAAGATCACCGGTGGCCCGGGCATCGACCTGTTCGGCGCCCCGTTGCACGAGCTGCCGCTGATCTGGGGCGAACTGATCGAGGCCGGTTTCGAAACCGGCCACGCCTACGGCAAGTCGAcc                                                                                                                                 <  8:152914/149‑1 (MQ=255)
                cAAGAAATACGACCTGTACACCAAGATCACCGGTGGCCAGCGCATCGACCTGTTCGGCGCCCAGTTGCACGAGCTGCCGCTGATCTGGGGCGAACTGATCGTGGCCGGTTTCGAAACCGGCCTCgatca                                                                                                                                              <  5:74188/129‑5 (MQ=255)
                cAAGAAATACGACCTGTACACCAAGATCACCGGTGGCCAGCGCATCGACCTGTTCGGCGCCCAGTTGCACGAGCTGCCGCTGATCTGGGGCGAACTGATCGTGGCCGGTTTCGAAACCGGCCTCgatca                                                                                                                                              >  6:74188/1‑125 (MQ=255)
                cAAGAAATACGACCTGTACACCAAGATCACCGGTGGCCAGCGCATCGACCTGTTCGGCGCCCAGTTGCACGAGCTGCCGCTGATCTGGGGCGAACTGATCGAGGCCGGTTTCGAAACCGGCCACGCCTACGGCAAGTCGACCCGCACgg                                                                                                                          >  5:171703/1‑149 (MQ=255)
                 aaGAAATACGACCTGTACACCAAGATCACCGGTGGCCAGCGCATCGACCTGTTCGGCGCCCAGTTGCACGAGCTGCCGCTGATCTGGGGCGAACTGCTCGAGGCCGGTTTCGAAACCGGCCACGCCGACGGCAAGTCGACCCGCACgg                                                                                                                          >  6:300813/1‑148 (MQ=255)
                  aGAAATACGACCTGTACACCAAGATCACCGGTGGCCAGCGCATCGACCTGTTCGGCGCCCAGTTGCACGAGCTGCCGCTGATCTGGGGCGAACTGATCGAGGCCGGTTTCGAAACCGGCCACGCCTACGGCAAGTCGACCCGCACGGt                                                                                                                         >  6:93916/1‑148 (MQ=255)
                                     cAAGATCACCGGTGGCCAGCGCATCGACCTGTTCGGCGCCCAGTTGCACGAGCTGCCGCTGATCTGGGGCGAACTGATCGAGGCCGGTTTCGAAACCGGCCACGCCTACGGCAAGTCGACCCGCACGGTAAAGt                                                                                                                    <  2:266510/134‑1 (MQ=255)
                                     cAAGATCACCGGTGGCCAGCGCATCGACCTGTTCGGCGCCCAGTTGCACGAGCTGCCGCTGATCTGGGGCGAACTGATCGAGGCCGGTTTCGAAACCGGCCACGCCTACGGCAAGTCGACCCGCACGGTAAAGt                                                                                                                    >  1:266510/1‑134 (MQ=255)
                                     cAAGATCACCGGTGGCCAGCGCATCGACCTGTTCGGCGCCCAGTTGCACGAGCTGCCGCTGATCTGGGGCGAACTGATCGAGGCCGGTTTCGAAACCGGCCACGCCTACGGCAAGTCGACCCGCACGGTAAAGTCGTGCGTGGgcagca                                                                                                     >  3:308364/1‑149 (MQ=255)
                                       aGATCACCGGTGGCCAGCGCATCGACCTGTTCGGCGCCCAGTTGCACGAGCTGCCGCTGATCTGGGGCGAACTGATCGAGGCCGGTTTCGAAACCGGCCACGCCTACGGCAAGTCGACCCGCACGGTAAAGTCGTGCGTGGGCAGCAcc                                                                                                   >  8:136091/1‑149 (MQ=255)
                                        gATCACCGGTGGCCAGCGCATCGCCCTGTTCGGCGCCCAGTTGCACGAGCTGCCGCTGATCTGGGGCGAACTGATCGAGGCCGGTTTCGAAACCGGCCACGCCTACGGCAAGTCGACCCGCACGGTAAAGTCGTGCGTGGGCAGCAccg                                                                                                  >  8:223652/1‑148 (MQ=255)
                                        gATCACCGGTGGCCAGCGCATCGACCTGTTCGGCGCCCAGTTGCACGAGCTGCCGCTGTTCTGGGGCGAACTGATCGAGGCCGGTTTCGAAACCGGCCACGCCTACGGCAAGTCGACCCGCACGGTAAAGTCGTGCGTGGGCAGCACCt                                                                                                  >  1:337568/1‑149 (MQ=255)
                                                   gCCAGCGCATCGACCTGTTCGGCGCCCAGTTGCACGAGCTGCCGCTGATCTGGGGCGAACTGATCGAGGCCGGTTTCCAAACCGGCCACGCCTACGGCAAGTCGACCCGCACGGTAAAGTCGTGCGTGGGCAGCACCTGGTGCCGTTAc                                                                                       <  1:185572/149‑1 (MQ=255)
                                                     cAGCGCATCGACCTGTTCGGCGCCCAGTTGCACGAGCTGCCGCTGATCTGGGGCGAACTGATCGAGGCCGGTTTCGAAACCGGCCACGCCTACGGCAAGTCGACCCGCACGGTAAAGTCGTGCGTGGGCAGCACCTGGTGCCGTTACgg                                                                                     >  2:146967/1‑149 (MQ=255)
                                                              gACCTGTTCGGCGCCCAGTTGCACGAGCTGCCGCTGATCTGGGGCGAACTGATCGAGGCCGGTTTCGACACCGGCCACGCCTACGGCAAGTCGACCCGCACGGTAAAGTCGTTCGTGGGCAGCACCTGGTGCCGTGACGGCGTGCAggc                                                                            >  8:57537/1‑148 (MQ=255)
                                                              gACCTGTTCGGCGCCCAGTTGCACGAGCTGCCGCTGATCTGGGGCGAACTGATCGAGGCCGGTTTCGAAACCGGCCACGCCTACGGCAAGTCGACCCGCACGGTAAAGTCGTGCGTGGGCAGCACCTGGTGCCGTTACGGCGTGCAGGa                                                                            <  5:93916/149‑1 (MQ=255)
                                                               aCCTGTTCGGCGCCCAGTTGCACGAGCTGCCGCTGATCTGGGGCGAACTGATCGAGGCCGGTTTCGAAACCGGCCACGCCTACGGCAAGTCGACCCGCACGGTAAAGTCGTGCGTGGGCAGCACCTGGTGCCGTTACGGCGTGCAGGa                                                                            >  4:68576/1‑148 (MQ=255)
                                                                   gTTCGGCGCCCAGTTGCACGAGCTGCCGCTGATCTGGGGCGAACTGATCGAGGCCGGTTTCGAAACCGGCCACGCCTACGGCAAGTCGACCCGCACGGTAAAGTCGTGCGTggg                                                                                                          >  7:115772/1‑114 (MQ=255)
                                                                         cgcCCAGTTGCACGAGCTGCCGCTGATCTGGGGCGAACTGATCGAGGCCGGTTTCGAAACCGGCCACGCCTACGGCAAGTCGACCCGCACGGTAAAGTCGTGCGTGGGCAGCACCTGGTGCCGTTACGGCGTGCAGGACAGTGTCGCCa                                                                 >  3:289948/1‑149 (MQ=255)
                                                                          gcCCAGTTGCACGAGCTGCCGCTGATCTGGGGCGAACGGATCGAGGCCGGTTTCGAAACCGGCCACGCCTACGGCAAGTCGACCCGCACGGTAAAGACGTGCGTGGGCAGCACCTGGTGCCGTTAAGGCGTGCCGGACAGTGTCGACAt                                                                >  4:176768/1‑149 (MQ=255)
                                                                            ccAGTTGCACGAGCTGCCGCTGATCTGGGGCGAACTGATCGAGGCCGGTTTCGAAACCGGCCACGCCTACGGCAAGTCGACCCGCACGGTAAAGTCGTGCGTGGGCAGCACCTGGTGCCGTTACGGCGTGCAGGACAGTGTCGCCATgg                                                              <  4:243136/149‑1 (MQ=255)
                                                                              aGTTGCACGAGCTGCCGCTGATCTGGGGCGAACTGATCGAGGCCGGTTTCGAAACCGGCCACGCCTACGGCAAGTCGACCCGCACGGTAAAGTCGTGCGTGGGCAGCACCTGGTGCCGTTACGGCGTGCAGGACAGTGTCGCCATGGcc                                                            <  1:74746/149‑1 (MQ=255)
                                                                               gTTGCACGAGCTGCCGCTGATCTGGGGCGAACTGATCGAGGCCGGTTTCGAAACCGGCCACGCCTACGGCAAGTCGACCCGCACGGTAAAGTCGTGCGTGGGCAGCACCTGGTGCCGTTACGGCGTGCAGGACAGTGTCGCCATGGccc                                                           >  4:30266/1‑149 (MQ=255)
                                                                                ttGCACGAGCTGCCGCTGATCTGGGGCGAACTGATCGAGGCCGGTTTCGAAACCGGCCACGCCTACGGCAAGTCGACCCGCACGGTAAAGTCGTGCGTGGGCAGCACCTGGTGCCGTTACGGCGTGCAGGACAGTGTCGCCATGGCCCt                                                          >  3:270166/1‑149 (MQ=255)
                                                                                      gAGCTGCCGCTGATCTGGGGCGAACTGATCGAGGCCGGTTTCGAAACCGGCCACGCCTACGGCAAGTCGACCCGCACGGTAAAGTCGTGCGTGGGCAGCACCTGGTGCCGTTACGGCGTGCAGGACAGTGTCGCCATGGCCCTGCGCCt                                                    <  6:171703/149‑1 (MQ=255)
                                                                                            ccGCTGATCTGGGGCGAACTGATCGAGGCCGGTTTCGAAACCGGCCACGCCTACGGCAAGTCGACCCGCACGGTAAAGTCGTGCGTGGGCAGCACCTGGTGCCGTTACGGCGTGCAGGACAGTGTCGCCATGGCCCTGCGCCTGGaa                                                <  3:68576/147‑1 (MQ=255)
                                                                                              gCTGATCTGGGGCGAACTGATCGAGGCCGGTTTCGAAACCGGCCACGCCTACGGCAAGTCGACCCGCACGGTAAAGTCGTGCGTGGGCAGCACCTGGTGCCGTTACGGCGTGCAGGACAGTGTCGCCATGGCCCTGCGCCTGGAAGATc                                            <  4:289948/149‑1 (MQ=255)
                                                                                               cTGATCTGGGGCGAACTGATCGAGGCCGGTTTCGAAACCGGCCACGCCTACGGCAAGTCGACCCGCACGGTAAAGTCGTGCGTGGGCAGCACCTGGTGCCGTTACGGCGTGCAGGACAGTGTCGCCATTGCCCTGCGCCTGGAAGATc                                            >  3:191532/1‑148 (MQ=255)
                                                                                               cTGATCTGGGGCGAACTGATCGAGGCCGGTTTCGAAACCGGCCACGCCTACGGCAAGTCGACCCGCACGGTAAAGTCGTGCGTGGGCAGCACCTGGTGCCGTTACGGCGTGCAGGACAGTGTCGCCATGGCCCTGCGCCTGGAAGATCg                                           <  7:136091/149‑1 (MQ=255)
                                                                                                      ggggCGAACTGATCGAGGCCGGTTTCGAAACCGGCCACGCCTACGGCAAGTCGACCCGCACGGTAAAGTCGTGCGTGGGCAGCACCTGGTGCCGTTACGGCGTGCAGGACAGTGTCGCCATGGCCCTGCGCCTGGAAGATAGCTACAAg                                    >  7:85956/1‑149 (MQ=255)
                                                                                                       gggCGAACTCATCGCGGCCGGTTGCGACACCGGCCACGCCTACGGCAAGTCGACCCGCACGGTAAAGTCGTGCGTGGGCAGCACCTGGTGCCGTTACGGCGTGCAGGACAGTGTCGCCATGGCCCTGCGCCGGGAAGATCGCTACAAg                                    <  8:141872/148‑1 (MQ=255)
                                                                                                         gCGAACTGATCGAGGCCGGTTTTGAAACCGGCCACGCCTACGGCAAGTCGACCCGCACGGTAAAGTCGTGCGTGGGCAGCACCTGGTGCCGTTACGGCGTGCAGGACAGTGTCGCCATGGCCCTGCGCCTGGAAGATCGCTACAAGGGc                                 >  7:216252/1‑149 (MQ=255)
                                                                                                           gAACTGATCGAGGCCGGTTTCGAAACCGGCCACGCCTACGGCAAGTCGACCCGCACGGTAAAGTCGTGCGTGGGCAGCACCTGGTGCCGTTACGGCGTGCAGGACAGTGTCGCCATGGCCCTGCGCCTGGAAGATCGCTACAAGGGCCt                               <  4:270166/149‑1 (MQ=255)
                                                                                                               taggcgtggCCGGTTTCGAAACCGGCCTCGCCTACGGCAAGTCGACCCGCACGGTAAAGTCGTGCGTGGGCAGCACCAGGTGCCGTTACGGCCTGCAGGACAGTGTCGCCATGGCCCAGCGCCGGGAACCTCGCTACAACGGGCGgcgc                           >  8:289549/8‑149 (MQ=255)
                                                                                                               tGATCGAGGCCGGTTTCGAAACCGGCCACGCCTACGGCAAGTCGACCCGCACGGTAAAGTCGTGCGTGGGCAGCACCTGGTGCCGTTACGGCGTGCAGGACAGTGTCGCCATGGCCCTGCGCCTGGAAGATCg                                           <  8:192006/133‑1 (MQ=255)
                                                                                                               tGATCGAGGCCGGTTTCGAAACCGGCCACGCCTACGGCAAGTCGACCCGCACGGTAAAGTCGTGCGTGGGCAGCACCTGGTGCCGTTACGGCGTGCAGGACAGTGTCGCCATGGCCCTGCGCCTGGAAGATCg                                           >  7:192006/1‑133 (MQ=255)
                                                                                                                aggcgtggCCGGTTTCGAAACCGGCCTCGCCTACGGCAAGTCGACCCGCACGGTAAAGTCGTGCGTGGGCAGCACCTGGTGCCGTTACGGCGTGCAGGACAGTGTCGCCATGGCCCTGCGCCTGGAAGATCGCTACAAGGGCCTGCGCa                          >  1:154685/7‑149 (MQ=255)
                                                                                                                  tCGAGGCCGGTTTCGAAACCGGCCACGCCTACGGCAAGTCGACCCGCAAGGTAAAGTCGTGCGTGGGCAGCAACTGGTGCCGTTACGGCGTGCAGGACAGTGTCGCCATGGCCCTGCGCCTGGAAGATCGCTACAAGGTCCTTCGCAGc                        >  8:149289/1‑149 (MQ=255)
                                                                                                                   cgtggCCGGTTTCGAAACCGGCCTCGCCTACGGCAAGTCGACCCGCACGGTAAAGTCGTGCGTGGGCAGCACCTGGTGCCGTTACGGCGTGCAGGACAGTGTCGCCATGGCCCTGCGCCTGGAAGATCGCTACAAGGGCCTGCGCAGcc                       <  7:289549/146‑1 (MQ=255)
                                                                                                                     tggCCGGTTTCGAAACCGGCCACGCCTACGGCAAGTCGACCCGCACGGTAAAGTCGTGCGTGGGCAGCACCTGGTGCCGTTACGGCGTGCAGGACAGTGTCGCCATGGCCCTGCGCCTGGAAGATCGCTACAAGGGCCTGCGCAGcccc                     >  6:64268/2‑149 (MQ=255)
                                                                                                                        ccGGTTGCGAAACGGGCCACGCCTACGGCAAGTCGACCCGCACGGTAAAGTCGTGCGTGGGCAGCACCTGGTGCCGTTACGGCGTGCAGGACAGTGTCGCCATGGCCCTGCGCCTGGAAGATCGCTACAAGGGCCTGCGCAGCCCCcac                  <  1:197931/149‑1 (MQ=255)
                                                                                                                         cGGTTTCGAAACCGGCCACGCCTACGGCAAGTCGACCCGCACGGTAAAGTCGTGCGTGGGCAGCACCTGGTGCCGTTACGGCGTGCAGGACAGTGTCGCCATGGCCCTGCGCCAGGAAGATCGCTACAAGGGCCTGCGCAGCCCCcac                  >  3:72678/1‑148 (MQ=255)
                                                                                                                            tttCTAAACCGGCCACGCCTACGGCAAGTCGACCCGCACGGTAAAGTCGTGCGTGGGCAGCACCTGGTGCCGTTACGGCGTGCAGGACAGTGTCGCCATGGCCCTGCGCCTGGAAGATCGCTACAAGGGCCTGCGCAGCCCCCACAAGc              <  1:146967/149‑1 (MQ=255)
                                                                                                                                gAAACCGGCCACGCCTACGGCAAGTCGACCCGCACGGTAAAGTCGTGCGTGGGCAGCACCTGGTGCCGTTACGGCGTGCAGGACAGTGTCGCCATGGCCCTGCGCCTGGAAGATCGCTACAAGGGCCTGCGCAGCCCCCACAAGCTCaa          <  4:308364/149‑1 (MQ=255)
                                                                                                                                   aCCGGCCACGCCTACGGCAAGTCGACCCGCACGGTAAAGTCGTGCGTGGGCAGCACCTGGTGCCGTTACGGCGTGCAGGACAGTGTCGCCATGGCCCTGCGCCTGGAAGATCGCTACAAGGGCCTGCGCAGCCCCCACAAGCTCAAGtt       >  4:177733/1‑149 (MQ=255)
                                                                                                                                       tccACGCCTACGGCAAGTCGACCCGCACGGTAAAGTCGTGCGTGGGCAGCACCTGGTGCCGTTACGGCGTGCAGGACAGTGTCGCCATGGCCCTGCGCCTGGAAGATCGCTACAGGGGCCTGCGCAGCCCCCACAAGCTCAAGTTcgcg   <  7:57537/148‑1 (MQ=255)
                                                                                                                                       gCCACGCCTACGGCAAGTCGACCCGCACGGTAAAGTCGTGCGTGGGCAGCACCTGGTGCCGTTACGGCGTGCAGGACAGTGTCGCCATGGCCCTGCGCCTGGAAGATCGCTACAAGGGCCTGCGCAGCCCCCACAAGCTCAAGTTcgcg   >  2:4940/1‑149 (MQ=255)
                                                                                                                                        ccACGCCTACGGCAAGTCGACCCGCACGGTAAAGTCGTGCGTGGGCAGCACCTGGTGCCGTTACGGCGTGCAGGACAGTGTCGCCATGGCCCTGCGCCAGGAAGATCGCTACAAGGGCCTGCGCAGCCCCCACAAGCTCAAGTTCGCgg  <  4:72678/149‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                                  
TCATCGGCCAGGTGGCCAAGAAATACGACCTGTACACCAAGATCACCGGTGGCCAGCGCATCGACCTGTTCGGCGCCCAGTTGCACGAGCTGCCGCTGATCTGGGGCGAACTGATCGAGGCCGGTTTCGAAACCGGCCACGCCTACGGCAAGTCGACCCGCACGGTAAAGTCGTGCGTGGGCAGCACCTGGTGCCGTTACGGCGTGCAGGACAGTGTCGCCATGGCCCTGCGCCTGGAAGATCGCTACAAGGGCCTGCGCAGCCCCCACAAGCTCAAGTTCGCGG  >  NC_002947/1905508‑1905792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑