Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,938,844 T→C 6.1% E71G (GAG→GGG)  PP_1738 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,938,8440TC 6.1% 194.9 / 2.1 65E71G (GAG→GGG) PP_1738hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (33/28);  new base C (2/2);  total (35/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

AGGTATTCATTGATGCTGCTGCTGTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCT‑GGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAAGAGGGTAGCCATAGCTGGCAGCGTCGATATCCCGCG  >  NC_002947/1938703‑1938991
                                                                                                                                             |                                                                                                                                                    
aGGTATTCATTGATGCTGCTGCTGTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGgcg                                                                                                                                               <  7:57819/149‑1 (MQ=255)
aGGTATTCATTGATGCTGCTGCTGTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGgc                                                                                                                                                <  4:128626/148‑1 (MQ=255)
 ggTATTCATTGATGCTGCTGCTGTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGgcg                                                                                                                                               >  3:207004/1‑148 (MQ=255)
  gTATTCATTGATGCTGCTGCTGTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGgcgc                                                                                                                                              >  2:89555/1‑148 (MQ=255)
    aTTCATTGATGCTGCTGCTGTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTTGCGCCCGGAGCGCGAg                                                                                                                                           >  1:130928/1‑149 (MQ=255)
        aTTGATGCTGCTGCTGTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTTGCGCCCGGAGCGCGAGTTCa                                                                                                                                       <  2:130928/149‑1 (MQ=255)
        aTTGATGCTGCTGCTGTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGtt                                                                                                                                         >  6:344334/1‑147 (MQ=255)
                 gctgctgTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGt                                                                                                                              >  5:92682/1‑149 (MQ=255)
                  ctgctgTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTc                                                                                                                             <  2:332907/149‑1 (MQ=255)
                     ctgTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCgg                                                                                                                           >  3:68026/1‑148 (MQ=255)
                        tAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACt                                                                                                                        <  6:92682/148‑1 (MQ=255)
                          gggCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGa                                                                                                                      <  1:89555/148‑1 (MQ=255)
                          gggCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAg                                                                                                                     >  7:119423/1‑149 (MQ=255)
                          gggCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAg                                                                                                                     >  4:49318/1‑149 (MQ=255)
                               cgcCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGAtt                                                                                                                <  3:49318/149‑1 (MQ=255)
                                  cGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCa                                                                                                             >  7:224867/1‑149 (MQ=255)
                                    gTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCAtc                                                                                                           <  8:119423/149‑1 (MQ=255)
                                       ccGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCAtcttc                                                                                                        >  8:311480/1‑149 (MQ=255)
                                        cGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCAtcgtcg                                                                                                       >  5:103042/1‑149 (MQ=255)
                                             atgatgAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGAGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCT‑ggg                                                                                                 <  4:68026/149‑1 (MQ=255)
                                              tgatgaAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCT‑ggg                                                                                                 >  3:115633/1‑148 (MQ=255)
                                                atgaAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCT‑GGGCAg                                                                                              <  5:344334/149‑1 (MQ=255)
                                                  gaAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCT‑GGGCAGCt                                                                                            >  6:138666/1‑149 (MQ=255)
                                                     gATCAGCGGCGCTTCGTGATACTGGAGGGCCACGCGGTACTTGAGCTTCTTCACTGCACAGAAGTTGTCGGGAAACGTGAACTCGCGCTCCGGGCTCAAGTTCAGGATCTAGTCGGACTGAGAGATTTCATCGTAGCT‑GCGCAGCTCAg                                                                                         >  2:1701/1‑149 (MQ=255)
                                                       tCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCT‑GGGCAGCGCAggg                                                                                       <  7:311480/149‑1 (MQ=255)
                                                        cAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCT‑GGGCAGCTCAggg                                                                                       <  4:207004/148‑1 (MQ=255)
                                                        cAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGATCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCT‑GGGCAGCTCAGGgc                                                                                      >  8:25115/1‑149 (MQ=255)
                                                          gcggcgCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCT‑GGGCAGCTCAGGgcgc                                                                                    <  5:138666/149‑1 (MQ=255)
                                                               gcTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCT‑GGGCAGCTCAGGGCGCTTGTc                                                                               >  6:169315/1‑149 (MQ=255)
                                                                 ttCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCT‑GGGCAGCTCAGGGCGCTTGTCcg                                                                             >  3:193600/1‑149 (MQ=255)
                                                                     tGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCT‑GGGCCGAGCAGGGCGCGTGTCgggcgg                                                                         >  8:146788/1‑149 (MQ=255)
                                                                          cTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCT‑GGGCAGCTCAGGGCGCTTGTCCGGCGGCgtgg                                                                    >  8:64955/1‑149 (MQ=255)
                                                                           tGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCT‑GGGCAGCTCAGGGCGCTTGTCCGGCGGCgtggt                                                                   >  4:267608/1‑149 (MQ=255)
                                                                           tGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCT‑GGGCAGCTCAGGGCGCTTGTCCGGCGGCgtggt                                                                   >  3:164190/1‑149 (MQ=255)
                                                                                 gCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCT‑GGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGAt                                                             <  4:164190/149‑1 (MQ=255)
                                                                                       cGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCT‑GGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGc                                                       >  4:250241/1‑149 (MQ=255)
                                                                                           aCTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCATGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCT‑GGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTc                                                    >  4:262879/1‑148 (MQ=255)
                                                                                               gAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCT‑GGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGaa                                                 <  7:64955/147‑1 (MQ=255)
                                                                                                 gCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCT‑GGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACggg                                             <  2:336479/149‑1 (MQ=255)
                                                                                                   ttcttcACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCT‑GGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGtt                                           >  2:275096/1‑149 (MQ=255)
                                                                                                     cttcACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCT‑GGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTg                                          <  4:193600/148‑1 (MQ=255)
                                                                                                     cttcACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCT‑GGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTg                                          <  3:250241/148‑1 (MQ=255)
                                                                                                           tGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCT‑GGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAagag                                   >  2:317035/1‑149 (MQ=255)
                                                                                                             cccAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCT‑GGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAAGAgg                                  <  8:224867/148‑1 (MQ=255)
                                                                                                                 gAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCT‑GGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAAGAGGGTAGc                             <  1:317035/149‑1 (MQ=255)
                                                                                                                     ttGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCT‑GGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAAGAgg                                  <  8:168476/140‑1 (MQ=255)
                                                                                                                     ttGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCT‑GGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAAGAgg                                  >  7:168476/1‑140 (MQ=255)
                                                                                                                       gTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCT‑GGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAAGAGGGTAGCCATAGc                       >  5:170132/1‑149 (MQ=255)
                                                                                                                        tCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCT‑GGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAAGAGGGTAGCCATAGCt                      >  3:251696/1‑149 (MQ=255)
                                                                                                                          gggCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCT‑GGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAAGAGGGTAGCCATAGCTgg                    <  3:267608/149‑1 (MQ=255)
                                                                                                                           ggCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGTGTGATTTCATCGTCGCT‑GGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAAGAGGGTAGCCATAGCTGGc                   <  6:103042/149‑1 (MQ=255)
                                                                                                                            gCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTAAAGCAGGACAGGGCGGTTGTCCGGCGGGGTGGTGGGGATGGTGGCCTCGAACGGGTTGGTAAGAGGGTAGCCATTGCTgg                    >  1:279557/1‑148 (MQ=255)
                                                                                                                            gCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCT‑GGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAaga                                    >  3:139657/1‑131 (MQ=255)
                                                                                                                            gCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCT‑GGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAaga                                    <  4:139657/131‑1 (MQ=255)
                                                                                                                                cGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCT‑GGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAAGAGGGTAGCCATAGCTGGCAGCGt              <  4:251696/149‑1 (MQ=255)
                                                                                                                                cGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCT‑GGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAAGAGGGTAGCCATAGCTGGCAGCGt              >  3:13429/1‑149 (MQ=255)
                                                                                                                                  tGAACTTGCGCCCGGAGCGCGAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCT‑GGGCAGCTCAGGgcgc                                                                                    <  6:258299/77‑1 (MQ=37)
                                                                                                                                  tGAACTTGCGCCCGGAGCGCGAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCT‑GGGCAGCTCAGGgcgc                                                                                    >  5:258299/1‑77 (MQ=37)
                                                                                                                                   gAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCT‑GGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGTtggtg                                                         <  5:248707/103‑1 (MQ=255)
                                                                                                                                   gAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGACGGACTGAGTCATTTCATCGTCGCT‑GGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGAtggtg                                                         >  6:248707/1‑103 (MQ=255)
                                                                                                                                    aaCTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCT‑GGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAAGAGGGTAGCCATAGCTgg                    <  2:32042/139‑1 (MQ=255)
                                                                                                                                    aaCTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCT‑GGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAAGAGGGTAGCCATAGCTgg                    >  1:32042/1‑139 (MQ=255)
                                                                                                                                         gcgcTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCT‑GGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAAGAGGGTAGCCATAGCTGGCAGCGTCGat          <  2:175868/144‑1 (MQ=255)
                                                                                                                                         gcgcTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCT‑GGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAAGAGGGTAGCCATAGCTGGCAGCGTCGat          >  1:175868/1‑144 (MQ=255)
                                                                                                                                            cTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCT‑GGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAAGAGGGTAGCCATAGCTGGCAGCGTCGATATCCcgcg  <  4:13429/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                    
AGGTATTCATTGATGCTGCTGCTGTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCT‑GGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAAGAGGGTAGCCATAGCTGGCAGCGTCGATATCCCGCG  >  NC_002947/1938703‑1938991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑