Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,941,690 A→T 5.3% T78S (ACG→TCG)  betX → choline/betaine/carnitine ABC transporter substrate‑binding protein BetX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,941,6900AT 5.3% 233.5 / 2.5 76T78S (ACG→TCG) betXcholine/betaine/carnitine ABC transporter substrate‑binding protein BetX
Reads supporting (aligned to +/- strand):  ref base A (33/39);  new base T (2/2);  total (35/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.20e-01

CCAAAGAAGTAAGCATTGGTTACGTGGATGGTTGGGCCGACAGCGTGGCGACCACCAACGTGGCTGCCGAAGTAATCAAGCAAAAACTCGGCTATGACGTGAAGTTGCAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACGGGCAAGCTCGATGCCATGTTGTCGGCCTGGTTGCCGGTGACTCACGGTGAGTACTGGGCCAAGAACAAGGACAACGTGGTCGACTACGGCCCGAACTTCAAGGACGCCAAGATCGGCCTGATCGTCCCCGAGTACGTCAAGGCA  >  NC_002947/1941544‑1941836
                                                                                                                                                  |                                                                                                                                                  
ccAAAGAAGTAAGCATTGGTTACGTGGATGGTTGGGCCGACAGCGTGGCGACCACCAACGTGGCTGCCGAAGTAATCAAGCAAAAACTCGGCTATGACGTGAAGTTGCAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACg                                                                                                                                                  >  2:335358/1‑149 (MQ=255)
  aaaGAAGTAAGCATTGGTTACGTGGATGGTTGGGCCGACAGCGTGGCGACCACCAACGTGGCTGCCGAAGTAATCAAGCAAAAACTCGGCTATGACGTGAAGTTGCAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACggg                                                                                                                                                >  8:174052/1‑149 (MQ=255)
    agaagTAAGCATTGGTTACGTGGATGGTTGGGCCGACAGCGTGGCGACCACCAACGTGGCTGCCGAAGTAATCAAGCAAAAACTCGGCTATGACGTGAAGTTGCAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACGGGc                                                                                                                                               >  2:112132/1‑148 (MQ=255)
         tAAGCATTGGTTACGTGGATGGTTGGGCCGACAGCGTGGCGACCACCAACGTGGCTGCCGAAGTAATCAAGCAAAAACTCGGCTATGACGTGAAGTTGCAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACg                                                                                                                                                  >  6:6995/1‑140 (MQ=255)
         tAAGCATTGGTTACGTGGATGGTTGGGCCGACAGCGTGGCGACCACCAACGTGGCTGCCGAAGTAATCAAGCAAAAACTCGGCTATGACGTGAAGTTGCAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACg                                                                                                                                                  <  5:6995/140‑1 (MQ=255)
           aGCATTGGTTACGTGGATGGTTGGGCCGACAGCGTGGCGACCACCAACGTGGCTGCCGAAGTAATCAAGCAAAAACTCGGCTATGACGTGAAGTTGCAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACGGGCAAGCTCGa                                                                                                                                       <  7:213477/149‑1 (MQ=255)
              aTTGGTTACGTGGATGGTTGGGCCGACAGCGTGGCGACCACCAACGTGGCTGCCGAAGTAATCAAGCAAAAACTCGGCTATGACGTGAAGTTGCAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACGGGCAAGCTCGATGc                                                                                                                                    >  2:102547/1‑149 (MQ=255)
                 ggTTACGTGGATGGTTGGGCCGACAGCGTGGCGACCACCAACGTGGCTGCCGAAGTAATCAAGCAAAAACTCGGCTATGACGTGAAGTTGCAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACGGGCAAGCTCGATGCCa                                                                                                                                  <  4:104053/148‑1 (MQ=255)
                  gTTACGTGGATGGTTGGGCCGACAGCGTGGCGACCACCAACGTGGCTGCCGAAGTAATCAAGCAAAAACTCGGCTATGACGTGAAGTTGCAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCATCGAGCTTGCCCGTTGCCac                                                                                                                                 <  7:289507/148‑2 (MQ=255)
                  gTTACGTGGATGGTTGGGCCGACAGCGTGGCGACCACCAACGTGGCTGCCGAAGTAATCAAGCAAAAACTCGGCTATGACGTGAAGTTGCAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACGGGCaa                                                                                                                                             <  4:306834/136‑1 (MQ=255)
                  gTTACGTGGATGGTTGGGCCGACAGCGTGGCGACCACCAACGTGGCTGCCGAAGTAATCAAGCAAAAACTCGGCTATGACGTGAAGTTGCAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACGGGCAAGCt                                                                                                                                          <  3:31644/139‑1 (MQ=255)
                  gTTACGTGGATGGTTGGGCCGACAGCGTGGCGACCACCAACGTGGCTGCCGAAGTAATCAAGCAAAAACTCGGCTATGACGTGAAGTTGCAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACGGGCAAGCt                                                                                                                                          >  4:31644/1‑139 (MQ=255)
                  gTTACGTGGATGGTTGGGCCGACAGCGTGGCGACCACCAACGTGGCTGCCGAAGTAATCAAGCAAAAACTCGGCTATGACGTGAAGTTGCAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACGGGCAAGCTCGATGCCAtg                                                                                                                                >  3:203769/1‑149 (MQ=255)
                  gTTACGTGGATGGTTGGGCCGACAGCGTGGCGACCACCAACGTGGCTGCCGAAGTAATCAAGCAAAAACTCGGCTATGACGTGAAGTTGCAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACGGGCAAGCTCGATGCCAt                                                                                                                                 <  1:102547/148‑1 (MQ=255)
                  gTTACGTGGATGGTTGGGCCGACAGCGTGGCGACCACCAACGTGGCTGCCGAAGTAATCAAGCAAAAACTCGGCTATGACGTGAAGTTGCAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACGGGCAAGCTCGATGCCAt                                                                                                                                 <  3:212878/148‑1 (MQ=255)
                  gTTACGTGGATGGTTGGGCCGACAGCGTGGCGACCACCAAAGTGGCTGCCGAAGTAATCAAGCAAAAACTCGGCTATGACGTGAAGTTGCAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACGGGCaa                                                                                                                                             >  3:306834/1‑136 (MQ=255)
                       gTGGATGGTTGGGCCGACAGCGTGGCGACCACCAACGTGGCTGCCGAAGTAATCAAGCAAAAACTCGGCTATGACGTGAAGTTGCAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACgg                                                                                                                                                 <  4:71318/127‑1 (MQ=255)
                       gTGGATGGTTGGGCCGACAGCGTGGCGACCACCAACGTGGCTGCCGAAGTAATCAAGCAAAAACTCGGCTATGACGTGAAGTTGCAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACgg                                                                                                                                                 >  3:71318/1‑127 (MQ=255)
                       gTGGATGGTTGGGCCGACAGCGTGGCGACCACCAACGTGGCTGCCGAAGTAATCAAGCAAAAACTCGGCTATGACGTGAAGTTGCAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACGGGCAAGCTCGATGCCATGTTGTc                                                                                                                           <  1:335358/149‑1 (MQ=255)
                        tGGATGGTTGGGCCGACAGCGTGGCGACCACCAACGTGGCTGCCGAAGTAATCAAGCAAAAACTCGGCTATGACGTGAAGTTGCAGGCCGTGGCTACGGGAATCATGTGGCAGGGTGTGGCAACGGGCAAGCTCGATGCCATGTTGTCg                                                                                                                          <  1:314006/149‑1 (MQ=255)
                        tGGATGGTTGGGCCGACAGCGTGGCGACCACCAACGTGGCTGCCGAAGTAATCAAGCAAAAACTCGGCTATGACGTGAAGTTGCAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACGGGCAAGCTCGATGCCATGTTGTCg                                                                                                                          <  4:203769/149‑1 (MQ=255)
                               ttGGGCCGACAGCGTGGCGACCACCAACGTGGCTGCCGAAGTAATCAAGCAAAAACTCGGCTATGACGTGAAGTTGCAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACGGGCAAGCTCGATGCCATGTTGTCGGCCTGGt                                                                                                                   >  5:193705/1‑149 (MQ=255)
                               ttGGGCCGACAGCGTGGCGACCACCAACGTGGCTGCCGAAGTAATCAAGCAAAAACTCGGCTATGACGTGAAGTTGCAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACGGGCAAGCTCGATGCCATGTTGTCGGCCTGGt                                                                                                                   >  3:193059/1‑149 (MQ=255)
                                    ccGACAGCGTGGCGACCACCAACGTGGCTGCCGAAGTAATCAAGCAAAAACTCGGCTATGACGTGTAGTTGGAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACGGGCAAGCTCGATGCCATGTTGTCGGCCTGGTTGCCg                                                                                                              <  1:112132/149‑1 (MQ=255)
                                    ccGACAGCGTGGCGACCACCAACGTGGCTGCCGAAGTAATCAAGCAAAAACTCGGCTATGACGTGAAGTTGCAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACGGGCAAGCTCGATGCCATGTTGTCGGCCTGGTTGCCg                                                                                                              <  5:309329/149‑1 (MQ=255)
                                    ccGACAGCGTGGCGACCACCAACGTGGCTGCCGAAGTAATCAAGCAAAAACTCGGCTATGACGTGAAGTTGCAGGCCGTGGCCACGGGAATCATGTGGCAGGGTATGGCAACGGGCAAGCTCGATGCCATGTTGTCGGCCTGGTTGCCg                                                                                                              <  6:279615/149‑1 (MQ=255)
                                      gACAGCGTGGCGACCACCAACGTGGCTGCCGAAGTAATCAAGCAAAAACTCGGCTATGACGTGAAGTTGCAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACGGGCAAGCTCGATGCCATGTTGTCGGCCTGGTTGCCGGt                                                                                                            <  1:60161/149‑1 (MQ=255)
                                        cAGCGTGGCGACCACCAACGTGGCTGCCGAAGTAATCAAGCAAAAACTCGGCTATGACGTGAAGTTGCAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACGGGCAAGCTCGATGCCATGTTGTCGGCCTGGTTGCCGGTGa                                                                                                          <  1:152725/149‑1 (MQ=255)
                                        cAGCGTGGCGACCACCAACGTGGCTGCCGAAGTAATCAAGCAAAAACTCGGCTATGACGTGAAGTTGCAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACGGGCAAGCTCGATGCCATGTTGTCGGCCTGGTTGCCGGTGa                                                                                                          <  6:193705/149‑1 (MQ=255)
                                               gCGACCACCAACGTGGCTGCCGAAGTAATCAAGCAAAAACTCGGCTATGACGTGAAGTTGCAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACGGGCAAGCTCGATGCCATGTTGTCGGCCTGGTTGCCGGTGACTCACgg                                                                                                   <  7:174052/149‑1 (MQ=255)
                                               gCGACCACCAACGTGGCTGCCGAAGTAATCAAGCAAAAACTCGGCTATGACGTGAAGTTGCAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACGGGCAAGCTCGATGCCATGTTGTCGGCCTGGTTGCCGGTGACTCACg                                                                                                    <  7:15586/148‑1 (MQ=255)
                                                  accaccAACGTGGCTGCCGAAGTAATCAAGCAAAAACTCGGCTATGACGTGAAGTTGCAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACGGGCAAGCTCGATGCCATGTTGTCGGCCTGGTTGCCGGTGACTCACGGTGa                                                                                                <  5:268529/149‑1 (MQ=255)
                                                     accaACGTGGCTGCCGAAGTAATCAAGCAAAAACTCGGCTATGACGTGAAGTTGCAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACGGGCAAGCTCGATGCCATGTTGTCGGCCTGGTTGCCGGTGACTCACGGTGAGTa                                                                                             >  8:276232/1‑149 (MQ=255)
                                                      ccaACGTGGCTGCCGAAGTAATCAAGCAAAAACTCGGCTATGACGTGAAGTTGCAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACGGGCAAGCTCGATGCCATGTTGTCGGCCTGGTTGCCGGTGACTCACGGTGa                                                                                                <  1:218000/145‑1 (MQ=255)
                                                      ccaACGTGGCTGCCGAAGTAATCAAGCAAAAACTCGGCTATGACGTGAAGTTGCAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACGGGCAAGCTCGATGCCATGTTGTCGGCCTGGTTGCCGGTGACTCACGGTGa                                                                                                >  2:218000/1‑145 (MQ=255)
                                                       caACGTGGCTGCCGAAGTAATCAAGCAAAAACTCGGCTATGACGTGAAGTTGCAGGTCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACGGGCAAGCTCGATGCCATGTTGTCGGCCTGGTTGCCGGTGACTCACGGTGAGTACt                                                                                           >  2:35638/1‑149 (MQ=255)
                                                                     aaGTAATCAAGCAAAAACTCGGCTATGACGTGAAGTTGCAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACGGGCAAGCTCGATGCCATGTTGTCGGCCTGGTTGCCGGTGACTCACGGt                                                                                                  <  1:78052/128‑1 (MQ=255)
                                                                     aaGTAATCAAGCAAAAACTCGGCTATGACGTGAAGTTGCAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACGGGCAAGCTCGATGCCATGTTGTCGGCCTGGTTGCCGGTGACTCACGGt                                                                                                  >  2:78052/1‑128 (MQ=255)
                                                                     aaGTAATCAAGCAAAAACTCGGCTATGACGTGAAGTTGCAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACGGGCAAGCTCGATGCCATGTTGTCGGCCTGGTTGCCGGTGACTCACGGTGAGTACTGGGCCAAGAACAAg                                                                             <  7:276232/149‑1 (MQ=255)
                                                                        tAATCAAGCAAAAACTCGGCTATGACGTGAAGTTGCAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACGGGCAAGCTCGATGCCATGTTGTCGGCCTGGTTGCCGGTGACTCACGGTGAGTACTGGGCCAAGAACAAGGa                                                                           >  7:57303/1‑148 (MQ=255)
                                                                           tCAAGCAAAGACTCGGCTATGACGTGAAGTTGCAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACGGGCAAGCTCGATGCCATGTTGTCGGCCTGGTTGCCGGTGACTCACGGTGAGTACTGGGCCAAGAACAAGGACAAc                                                                       >  8:201739/1‑149 (MQ=255)
                                                                           tCAAGCAAAAACTCGGCTATGACGTGAAGTTGCAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCATCGAGCTTGCCCGTTGCCacac                                                                                                                               >  6:183188/1‑90 (MQ=37)
                                                                           tCAAGCAAAAACTCGGCTATGACGTGAAGTTGCAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCATCGAGCTTGCCCGTTGCCacac                                                                                                                               <  5:183188/93‑4 (MQ=37)
                                                                           tCAAGCAAAAACTCGGCTATGACGTGAAGTTGCAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACGGGCAAGCTCGATGCCATGTTGTCGGCCTGGTTGCCGGTGACTCACGGTGAGTACTGGGCCAAGAACaa                                                                              >  3:232222/1‑142 (MQ=255)
                                                                           tCAAGCAAAAACTCGGCTATGACGTGAAGTTGCAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACGGGCAAGCTCGATGCCATGTTGTCGGCCTGGTTGCCGGTGACTCACGGTGAGTACTGGGCCAAGAACaa                                                                              <  4:232222/142‑1 (MQ=255)
                                                                             aaGCAAAAACTCGGCTATGACGTGAAGTTGCAGGCCGTGGCCACGTGAATCATGTCGCAGGGTGTGGCAACGGGCAAGCTCGATGCAATGTTGTCGGCCTGTTTGACGGTGACTAACGGTGAGTACTGGGCCAAGAACAAGGACAACGt                                                                     >  1:346480/1‑149 (MQ=255)
                                                                             aaGCAAAAACTCGGCTATGACGTGAAGTTGCAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACGGGCAAGCTCGATGCCATGTTGTCGGCCTGGTTGCCGGTGACTCACGGTGAGTACTGGGCCAAGAACAAGGACAACGt                                                                     >  1:234226/1‑149 (MQ=255)
                                                                              aGCAAAAACTCGGCTATGACGTGAAGTTGCAGGTCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACGGGCAAGCTCGATGCCATGTTGTCGGCCTGGTTGCCGGTGACTCACGGTGAGTACTGGGCCAAGAACAAGGACAACGTg                                                                    <  1:35638/149‑1 (MQ=255)
                                                                                  aaaaCTCGGCTATGACGTGAAGTTGCAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACGGGCAAGCTCGATGCCATGTTGTCGGCCTGGTTGCCGGTGACTCACGGTGAGTACTGGGCCAAGAACAAGGACAACGTGGTc                                                                 >  7:159263/1‑148 (MQ=255)
                                                                                  aaaaCTCGGCTATGACGTGAAGTTGCAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACGGGCAAGCTCGATGCCATGTTGTCGGCCTGGTTGCCGGTGACTCACGGTGAGTACTGGGCCAAGAACAAGGACAACGTGGTCg                                                                >  1:65812/1‑149 (MQ=255)
                                                                                              tGACGTTAAGTTGCAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACGGGCAAGCTCGATGCCATGTTGTCGGCCTGGTTGCCGGTGACTCACGGTGAGTACTGGGCCAAGAACAAGGACAACGTGGTCGACTACGGCCCg                                                     <  6:181192/148‑1 (MQ=255)
                                                                                               gACGTGAAGTTGCAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACGGGCAAGCTCGATGCCATGTTGTCGGCCTGGTTGCCGGTGACTCACGGTGAGTACTGGGCCAAGAACAAGGACAACGTGGTCGACTACGGCCCGaa                                                   >  2:128602/1‑149 (MQ=255)
                                                                                                     aaGTTGCAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACGGGCAAGCTCGATGCCATGTTGTCGGCCTGGTTGCCGGTGACTCACGGTGAGTACTGGGCCAAGAACAAGGACAACGTGGTCGACTACGGCCCGAACTTCaa                                             <  2:234226/149‑1 (MQ=255)
                                                                                                      atttGCAGTCCGTGGCCACGTGAATCATGTGGCAGGGTGTGGCAACTGGCAATATCGATGCAATGTTGTCTGCGTGGTTGCCGGTGACTCACGGTTATTACTGGGCCATGAACACGGTCTACGTGGTCGACTACGGCCCGAACTTCaa                                             <  2:346480/146‑1 (MQ=255)
                                                                                                       gTTGCAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACGGGCAAGCTCGATGCCATGTTGTCGGCCTGGTTGCCGGTGACTCACGGt                                                                                                  >  8:188182/1‑94 (MQ=255)
                                                                                                       gTTGCAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACGGGCAAGCTCGATGCCATGTTGTCGGCCTGGTTGCCGGTGACTCACGGt                                                                                                  <  7:188182/94‑1 (MQ=255)
                                                                                                          gCAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACGGGCAAGCTCGATGCCATGTTGTCGGCCTGGTTGCCGGTGACTCACGGTGAGTACTGGGCCAAGaa                                                                                 <  4:198767/108‑1 (MQ=255)
                                                                                                           cAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACGGGCAAGCTCGATGCCATGTTGTCGGCCTGGTTGCCGGTGACTCACGGTGAGTACTGGGCCAAGAACAAGGACAACGTGGTCGACTACGGCCCGAACTTCAAGGACGc                                       >  2:301374/1‑149 (MQ=255)
                                                                                                            aGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACGGGCAAGCTCGATGCCATGTTGTCGGCCTGGTTGCCGGTGACACACGGTGAGTACTGGGcc                                                                                      <  6:198083/101‑1 (MQ=255)
                                                                                                            aGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACGGGCAAGCTCGATGCCATGTTGTCGGCCTGGTTGCCGGTGACACACGGTGAGTACTGGGcc                                                                                      >  5:198083/1‑101 (MQ=255)
                                                                                                                 gTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACGGGCAAGCTCGATGCCATGTTGTCGGCCTGGTTGCCGGTGACTCACGGTGAGTACTGGGCCAAGAACAAGGACAACGTGGTCGACTACGGCCCGAACTTCAAGGACGCCAAGAt                                 <  7:201739/149‑1 (MQ=255)
                                                                                                                         gggAATCATGTGGCAGGGTGTGGCAACGGGCAAGCTCGATGCCATGTTGTCGGCCTGGTTGCCGGTGACTCACGGTGAGTACTGGGCCAAGAACAAGGACAACGTGGTCGACTACGGCCCGAACTTCAAGGACGCCAAGATCGGCCTGa                         >  7:110497/1‑149 (MQ=255)
                                                                                                                         gggAATCATGTGGCAGGGTGTGGCAACGGGCAAGCTCGATGCCATGTTGTCGGCCTGGTTGCCGGTGACTCACGGTGAGTACTGGGCCAAGAACAAGGACAACGTGGTCGACTACGGCCCGAACTTCAAGGACGCCAAGATCGGCCTGa                         <  1:128602/149‑1 (MQ=255)
                                                                                                                         gggAATCATGTGGCAGGGTGTGGCAACGGGCAAGCTCGATGCCATGTTGTCGGCCTGGTTGCCGGTGACTCACGGTGAGTACTGGGCCAAGAACAAGGACAACGTGGTCGACTACGGCCCGAACTTCAAGGACGCCAAGATCGGCCTGa                         <  4:193059/149‑1 (MQ=255)
                                                                                                                          ggAATCATGTGGCAGGGTGTGGCAACGGGCAAGCTCGATGCCATGTTGTCGGCCTGGTTGCCGGTGACTCACGGTGAGTACTGGGCCAAGAACAAGGACAACg                                                                      <  7:61567/103‑1 (MQ=255)
                                                                                                                          ggAATCATGTGGCAGGGTGTGGCAACGGGCAAGCTCGATGCCATGTTGTCGGCCTGGTTGCCGGTGACTCACGGTGAGTACTGGGCCAAGAACAAGGACAACg                                                                      >  8:61567/1‑103 (MQ=255)
                                                                                                                            aaTCATGTGGCAGGGTGTGGCAACGGGCAAGCTCGATGCCATGTTGTCGGCCTGGTTGCCGGTGACTCACGGTGAGTACTGGGCCAAGAACAAGGACAACGTGGTCGACTACGGCCCGAACTTCAAGGACGCCAAGATCGGCCTGATCg                      >  7:195161/1‑149 (MQ=255)
                                                                                                                              tCATGTGGCAGGGTGTGGCAACGGGCAAGCTCGATGCCATGTTGTCGGCCTGGTTGCCGGTGACTCACGGTGAGTACTGGGCCAAGAACAAGGACAACGTGGTCGACTACGGCCCGAACTTCAAGGACGCCAAGATCGGCCTGATCGTc                    >  6:272533/1‑149 (MQ=255)
                                                                                                                                  gtgGCAGGGTGTGGCAACGGGCAAGCTCGATGCCATGTTGTCGGCCTGGTTGCCGGTGACTCACGGTGAGTACTGGGCCAAGAACAAGGACAACGTGGTCGACTACGGCCCGAACTTCAAGGACGCCAAGATCGGCCt                           <  2:346048/138‑1 (MQ=255)
                                                                                                                                  gtgGCAGGGTGTGGCAACGGGCAAGCTCGATGCCATGTTGTCGGCCTGGTTGCCGGTGACTCACGGTGAGTACTGGGCCAAGAACAAGGACAACGTGGTCGACTACGGCCCGAACTTCAAGGACGCCAAGATCGGCCTGagatcgga                  >  1:346048/1‑140 (MQ=255)
                                                                                                                                   tgGCAGGGTGTGGCAACGGGCAAGCTCGATGCCATGTTGTCGGCCTGGTTGCCGGTGACTCACGGTGAGTACTGGGCCAAGAACAAGGACAACGTGGTCGACTACGGCCCGAACTTCAAGGACGCCAAGATCGGCCTGATCGTCCCCGa               <  8:195161/149‑1 (MQ=255)
                                                                                                                                    ggCAGGGTGTGGCAACGGGCAAGCTCGATGCCATGTTGTCGGCCTGGTTGCCGGTGACTCACGGTGAGTACTGGGCCAAGAACAAGGACAACGTGGTCGACTACGGCCCGAACTTCAAGGACGCCAAGATCGGCCTGATCGTCCCCGAg              >  7:33114/1‑149 (MQ=255)
                                                                                                                                         ggTGTGGCAACGGGCAAGCTCGATGCCATGTTGTCGGCCTGGTTGCCGGTGACTCACGGTGAGTACTGGGCCAAGAACAAGGACAACGTGGTCGACTACGGCCCGAACTTCAAGGACGcc                                      <  6:81922/120‑1 (MQ=255)
                                                                                                                                         ggTGTGGCAACGGGCAAGCTCGATGCCATGTTGTCGGCCTGGTTGCCGGTGACTCACGGTGAGTACTGGGCCAAGAACAAGGACAACGTGGTCGACTACGGCCCGAACTTCAAGGACGcc                                      >  5:81922/1‑120 (MQ=255)
                                                                                                                                            atgGCATCGAGCTTGCCCGTTGCCATGTTGTCGGCCTGGTTGCCGGTGACTCACGGTGAGTACTGGGCCAAGAACAAGGACAACGTGGTCGACTACGGCCCGAACTTCAAGGACGCCAAGATCGGCCTGATCGTCCCCGAGTACGTc        >  3:140934/2‑147 (MQ=255)
                                                                                                                                                cAACGGGCAAGCTCGATGCCATGTTGTCGGCCTGGTTGCCGGTGACTCACGGTGAGTACTGGGCCAAGAACAAGGACAACGTGGTCGACTACGGCCCGAACTTCAAGGACGCCAAGATCGGCCTGATCGTCCCCGAGTACGTCAAGGCa  <  8:57303/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CCAAAGAAGTAAGCATTGGTTACGTGGATGGTTGGGCCGACAGCGTGGCGACCACCAACGTGGCTGCCGAAGTAATCAAGCAAAAACTCGGCTATGACGTGAAGTTGCAGGCCGTGGCCACGGGAATCATGTGGCAGGGTGTGGCAACGGGCAAGCTCGATGCCATGTTGTCGGCCTGGTTGCCGGTGACTCACGGTGAGTACTGGGCCAAGAACAAGGACAACGTGGTCGACTACGGCCCGAACTTCAAGGACGCCAAGATCGGCCTGATCGTCCCCGAGTACGTCAAGGCA  >  NC_002947/1941544‑1941836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑