| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,951,644 | G→A | 10.8% | P633S (CCG→TCG) | mnmC ← | methyltransferase/FAD‑dependent oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,951,644 | 0 | G | A | 10.8% | 95.5 / 3.2 | 37 | P633S (CCG→TCG) | mnmC | methyltransferase/FAD‑dependent oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base G (15/18); new base A (2/2); total (17/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.42e-01 | |||||||||||
ACCTATGTGCTTTTCGAGAGTTTTAGAATGTTTGGTTATATGTGGCCAGGCAGGCAACCCGATGCAATCATTTCCCCCGGATCAACCGGCGCAATCCAAACCGGTTTGGATGGCAAGCCTGCGCCACTGCCCGCGGCAATGGCAGCGGTTCCCCACATACCCAGGCCGCCACCAGCTCGCCACTCAACGGTGCCGTGATCAACCCGCGTGAGCCATGCCCGCTGTTCACATACAACCCGCCCAGCCAAGGGCAGGGCACATCCGGTACTTGCCGCGCAT > NC_002947/1951509‑1951787 | aCCTATGTGCTTTTCGAGAGTTTTAGAATGTTTGGTTATATGTGGCCAGGCAGGCAACCCGATGCAATCATTTCCCCCGGATCAACCGGCGCAATCCAAACCGGTTTGGATGGCAAGCCTGCGCCACTGCCCGCGGCAATGGCAGCg < 7:62510/147‑1 (MQ=255) tATGTGCTTTTCGAGAGTTTTAGAATGTTTGGTTATATGTGGCCAGGCAGGCAACCCGATGCAATCATTTCCCCCGGATCAACCGGCGCAATCCAAACCGGTTTGGATGGCAAGCCTGCGCCACTGCCCGCGGCAATg > 6:18006/1‑138 (MQ=255) tATGTGCTTTTCGAGAGTTTTAGAATGTTTGGTTATATGTGGCCAGGCAGGCAACCCGATGCAATCATTTCCCCCGGATCAACCGGCGCAATCCAAACCGGTTTGGATGGCAAGCCTGCGCCACTGCCCGCGGCAATg < 5:18006/138‑1 (MQ=255) tgCTTTTCGAGAGTTTTAGAATGTTTGGTTATATGTGGCCAGGCAGGCAACCCGATGCAATCATTTCCCCCGGATCAACCGGCGCAATCCAAACCGGTTTGGATGGCAAGCCTGCGCCACTGCCCGCGGCAATGGCAGCGGTTCCCca < 2:182450/148‑1 (MQ=255) gCTTTTCGAGAGTTTTAGAATGTTTGGTTATATGTGGCCTGGCAGGCAACCCGATGCAATCATTTCCCCCGGATCAACCGGCGCAATCCAAACCGGTTTGGATGGCAAGCCTGCGCCACTGCCCGCGGCAATGGCAGCGGTTCCCcaca < 6:257972/149‑1 (MQ=255) cTTTTCGAGAGTTTTAGAATGTTTGGTTATATGTGGCCAGGCAGGCAACCCGATGCAATCATTTCCCCGGCTAAAACCGCCCAAAACAAAACGGGTTTGGATGCCAGCCATCCGCCCATCCCGCCGGCATTGGCACCGGTACCCcaaa > 7:200480/1‑146 (MQ=255) agagTTTTAGAATGTTTGGTTATATGTGGCCAGGCAGGCAACCCGATGCAATCATTTCCCCCGGATCAACCGGCGCAATCCAAACCGGTTTGGATGGCAAGCCTGCGCCACTGCCCGCGGCAATGGCAGCGGTTCCCCACATACCCAgg > 2:281413/1‑149 (MQ=255) tttGGTTATATGTGGCCAGGCAGGCAACCCGATGCAATCATTTCCCCCGGATCAACCGGCGCAATCCAAACCGGTTTGGATGGCAAGCCTGCGCCACTGCCCGCGGCAATGGCAGCGGTTCCCCACATACCCAGGCCGCCACCAGCTc < 6:182345/148‑1 (MQ=255) ggcaggcaAGCGGATGCAATCATTTCCCCAGGATCAACCGGCGCACTCCAAACCGGTTTGGATGGCAAGCCTGCGCCACTGCCCGCGGCAATGGCAGCGGTTCCCCACATACCCAGGCCGCCACCAGCTCGCCACTCAACGGTGCCg < 3:124092/147‑1 (MQ=255) gaaggcaACCCGATGCAATCATTTCCCCCGGATCAACCGGCGCAATCCAACCCGTTTTGGATGGCAAGCCTGCGCCACTGCCCGCGGCAATGGCAGCGGTTCCCCACATACCCAGGCCGCCACCAGCTCGCCACTCAACGGTGCCGTGa < 1:336253/147‑1 (MQ=255) caggcaACCCGCTGCAATCATTTCCCCCGGATCAACCGGCGCAATCCAAACCGGTTTGGATGGCAAGCCTGCGCCACTGCCCGCGGCAATGGCAGCGGTTCCCCACATACCCAGGCCGCCACCAGCTCGCCACTCAACGGTGCCGTGAt < 6:26423/149‑1 (MQ=255) aCCCGATGCAATCATTTCCCCCGGATCAACCGGCGCAATCCAAACCGGTTTGGATGGCAAGCCTGCGCCACTGCCCGCGGCAATGGCAGCGGTTCCCCACATACCCAGGCCGCCACCAGCTCGCCACTCAACGGTGCCGTGATCAAccc < 1:119957/149‑1 (MQ=255) ccGATGCAATCATTTCCCCCGGATCAACCGGCGCAATCCAAACCGGTTTGGATGGCAAGCCTGCGCCACTGCCCGCGGCAATGGCAGCGGTTCCCCACATACCCAGGCCGCCACCAGCTCGCCACTCAACGGTGCCGTGATCAACCcgc < 6:250104/149‑1 (MQ=255) aaTCATTTCCCCCGGATCAACCGGCGCAATCCAAACCGGTTTGGATGGCAAGCCTGCGCCACTGCCCGCGGCAATGGCAGCGGTTCCCCACATACCCAGGCCGCCACCAGCTCGCCACTCAACGGTGCCGTGATCAACCCGCGTGAGc > 7:315442/1‑148 (MQ=255) cATTTCCCCCGGATCAACCGGCGCAATCCAAACCGGTTTGGATGGCAAGCCTGCGCCACTGCCCGCGGCAATGGCAGCGGTTCCCCACATACCCAGGCCGCCACCAGCTCGCCACTCAACGGTGc > 2:155290/1‑125 (MQ=255) cATTTCCCCCGGATCAACCGGCGCAATCCAAACCGGTTTGGATGGCAAGCCTGCGCCACTGCCCGCGGCAATGGCAGCGGTTCCCCACATACCCAGGCCGCCACCAGCTCGCCACTCAACGGTGc < 1:155290/125‑1 (MQ=255) cccccGGATCAACCGGCGCAATCCAAACCGGTTTGGATGGCAAGCCTGCGCCACTGCCCGCGGCAATGGCAGCGGTTCCGCACATTCCCAGGCCGCCACCAGCTAGCCACTCAACGGTGCCGTGATCAACCCGCGTGAGCCATGCCCGc > 5:89298/1‑149 (MQ=255) cGGATCAACCGGCGCAATCCAAACCGGTTTGGATGGCAAGCCTGCGCCACTGCCCGCGGCAATGGCAGCGGTTCCCCACATACCCAGGCCGCCACCAGCTCGCCACTCAACGGTGCCGTGATCAACCCGCGTGAGCCATGc > 8:39418/1‑141 (MQ=255) cGGATCAACCGGCGCAATCCAAACCGGTTTGGATGGCAAGCCTGCGCCACTGCCCGCGGCAATGGCAGCGGTTCCCCACATACCCAGGCCGCCACCAGCTCGCCACTCAACGGTGCCGTGATCAACCCGCGTGAGCCATGc < 7:39418/141‑1 (MQ=255) cGGATCAACCGGCGCAATCCAAACCGGTTTGGATGGCAAGCCTGCGCCACTGCCCGCGGCAATGGCAGCGGTTCCCCACATACCCAGGCCGCCACCAGCTCGCCACTCAACGGTGCCGTGATCAACCCGCGTGAGCCATGCCCGCTGtt < 1:281413/149‑1 (MQ=255) ggCGCAATCCAAACCGGTTTGGATGGCAAGCCTGCGCCACTGCCCGCGGCAATGGCAGCGGTTCCCCACATACCCAGGCCGCCACCAGCTCGCCACTCAACGGTGCCGTGATCAACCCGCGTGAGCCATGCCCGCTGTTCACATACAAc < 6:18342/149‑1 (MQ=255) ttgccaTCCAAACCGGTTTGGATGGCAAGCCTGCGCCACTGCCCGCGGCAATGGCAGCGGTTCCCCACATACCCAGGCCGCCACCAGCTCGCCACTCAACGGTGCCGTGATCAACCCGCGTGAGCCATGCCCGATGTTCACATTCAAcc > 6:269941/6‑149 (MQ=255) ccAAACCGGTTTGGATGGCAAGCCTGCGCCACTGCCCGCGGCAATGGCAGCGGTTccc < 4:171051/58‑1 (MQ=255) ccAAACCGGTTTGGATGGCAAGCCTGCGCCACTGCCCGCGGCAATGGCAGCGGTTccc > 3:171051/1‑58 (MQ=255) aaaCCGGTTTGGATGGCAAGCCTGCGCCACTGCCCGCGGCAATGGCAGCGGTTCCCCACATACCCAGGCCGCCACCAGCTCGCCACTCAACGGTGCCGTGATCAACCCGCGTGAGCCATGCCCGCTGTTCACATACAACCCGCCCAGc > 3:284807/1‑148 (MQ=255) aCCGGTTTGGATGGCAAGCCTGCGCCACTGCCCGCGGCAATGGCAGCGGTTCCCCACATACCCAGGCCGCCACCAGCTCGCCACTCAACGGTGCCGTGATCAACCCGCGTGAGCCATGCCCGCTGTTCACATACAACCCGCCCAGCCaa < 1:204908/149‑1 (MQ=255) ggATGGCAAGCCTGCGCCACTGCCCGCGGCAATGGCAGCGGTTCCCCACATACCCAGGCCGCCACCAGCTCGCCACTCAACGGTGCCGTGATCAACCCGCGTGAGCCATGCCCGCTGTTCACATACAACCCGCCCa < 8:161134/136‑1 (MQ=255) ggATGGCAAGCCTGCGCCACTGCCCGCGGCAATGGCAGCGGTTCCCCACATACCCAGGCCGCCACCAGCTCGCCACTCAACGGTGCCGTGATCAACCCGCGTGAGCCATGCCCGCTGTTCACATACAACCCGCCCa > 7:161134/1‑136 (MQ=255) tGGAAAGCCTGCTCCACTGCCCGCGGCAATGGCAGCTGTTCCCCACATACCCAGCCCGCCACCAGCTCGCCACTCAACGGTGCCGTGATCAACCCGCGTGAGCCATGCCCGCTTTTCACATACAACCCGCCCAGCCAAGGGCAGGGcac < 6:89298/149‑1 (MQ=255) cgcTGCCCGCGACAATGGCAGCGGTTCCCCACATACCCAGGCCGCCACCAGCTCGCCACTCAACGGTGCCGTGATCAACCCGCGTGAGCCATGCCCGCTGTTCACATACAACCCGCCCAGCCAagggcagg > 4:249307/3‑131 (MQ=255) cgcTGCCCGCGACAATGGCAGCGGTTCCCCACATACCCAGGCCGCCACCAGCTCGCCACTCAACGGTGCCGTGATCAACCCGCGTGAGCCATGCCCGCTGTTCACATACAACCCGCCCAGCCAagggcagg > 4:244806/3‑131 (MQ=255) cgcTGCCCGCGACAATGGCAGCGGTTCCCCACATACCCAGGCCGCCACCAGCTCGCCACTCAACGGTGCCGTGATCAACCCGCGTGAGCCATGCCCGCTGTTCACATACAACCCGCCCAGCCAagggcagg < 3:249307/129‑1 (MQ=255) cgcTGCCCGCGACAATGGCAGCGGTTCCCCACATACCCAGGCCGCCACCAGCTCGCCACTCAACGGTGCCGTGATCAACCCGCGTGAGCCATGCCCGCTGTTCACATACAACCCGCCCAGCCAagggcagg < 3:244806/129‑1 (MQ=255) cTGCCCGCGGCAATGGCAGCGGTTCCCCACATACCCAGGCCGCCACCAGCTCGCCACTCAACGGTGCCGTGATCAACCCGCGTGAGCCATGCCCGCTGTTCACATACAACCCGCCCAGCCAAGGGCAGGGCACATCCGGTACTTGCcgc > 3:233737/1‑149 (MQ=255) cccGCGGCAATGGCAGCGGTTCCCCACATACCCAGGCCGCCACCAGCTCGCCACTCAACGGTGCCGTGATCAACCCGCGTGAGCCATGCCCGCTGTTCACATACAACCCGCCCAGCCAAGGGCAGGGCACATCCGGTACTTGCCGCGCa > 4:218655/1‑149 (MQ=255) ccGCGGCAATGGCAGCGGTTCCCCACATACCCAGGCCGCCACCAGCTCGCCACTCAACGGTGCCGTGATCAACCCGCGTGAGCCATGCCCGCTGTTCACATACAACCCGCCCAGCCAAGGGCAGGGCACATCCGGTACTTGCCGCGCAt > 1:49422/1‑149 (MQ=255) ccGCGGCAATGGCAGCGGTTCCCCACATACCCAGGCCGCCACCAGCTCGCCACTCAACGGTGCCGTGATCAACCCGCGTGAGCCATGCCCGCTGTTCACATACAACCCGCCCAGCCAAGGGCAGGGCACATCCGGTACTTGCCGCGCAt > 8:54661/1‑149 (MQ=255) | ACCTATGTGCTTTTCGAGAGTTTTAGAATGTTTGGTTATATGTGGCCAGGCAGGCAACCCGATGCAATCATTTCCCCCGGATCAACCGGCGCAATCCAAACCGGTTTGGATGGCAAGCCTGCGCCACTGCCCGCGGCAATGGCAGCGGTTCCCCACATACCCAGGCCGCCACCAGCTCGCCACTCAACGGTGCCGTGATCAACCCGCGTGAGCCATGCCCGCTGTTCACATACAACCCGCCCAGCCAAGGGCAGGGCACATCCGGTACTTGCCGCGCAT > NC_002947/1951509‑1951787 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |