| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,975,987 | C→T | 7.1% | Q307* (CAG→TAG) | pheA → | bifunctional chorismate mutase/prephenate dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,975,987 | 0 | C | T | 7.1% | 205.3 / 4.7 | 70 | Q307* (CAG→TAG) | pheA | bifunctional chorismate mutase/prephenate dehydratase |
| Reads supporting (aligned to +/- strand): ref base C (36/29); new base T (2/3); total (38/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.54e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.29e-01 | |||||||||||
GAAGATCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGA‑CCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGATCAAGGCGGTGCTCGAGCAGATCAGCCAGGAGGCTGTGGCGC > NC_002947/1975839‑1976134 | gaagaTCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATc < 7:156601/149‑1 (MQ=255)gaagaTCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATc < 7:152285/149‑1 (MQ=255) aTCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGaa > 2:214772/1‑149 (MQ=255) gAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACgg > 5:193699/1‑149 (MQ=255) gAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACg < 8:21709/148‑1 (MQ=255) gACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTGATAGAACGGCac < 5:332215/149‑2 (MQ=255) gACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTGATAGAACGGCac < 3:232689/149‑2 (MQ=255) aCCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATc < 6:285361/149‑1 (MQ=255) cGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTGATAGAACGGCacc > 4:72328/1‑145 (MQ=255) cGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTGATAGAACGGCacc < 3:72328/147‑3 (MQ=255) cGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGa < 7:185942/149‑1 (MQ=255) gCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGAc < 2:128680/149‑1 (MQ=255) cGGCCAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGAGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCAAGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTg > 8:82622/1‑149 (MQ=255) aCTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGA‑CCCGc < 6:198886/149‑1 (MQ=255) gcgcTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGA‑CCCGCATCgaga > 2:152619/1‑149 (MQ=255) cTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGA‑CCCGCATCGAGAccc > 1:232054/1‑149 (MQ=255) ttCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGA‑CCCGCATCGAGAcccg > 3:258717/1‑149 (MQ=255) ccTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGA‑CCCGCATCGAGAcccgc < 6:193699/148‑1 (MQ=255) tgatgaTCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGA‑CCCGCATCGAGAcccgcccg > 4:14703/1‑149 (MQ=255) gatgatCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGA‑CCCGCATCGAGACCCGCCCGt > 8:230028/1‑149 (MQ=255) gatgatCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGA‑CCCGCATCGAGACCCGCCCGt > 1:97321/1‑149 (MQ=255) aCCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGA‑CCCGCATCGAGACCCGCCCGTCGCGCAGCGGc < 1:193258/149‑1 (MQ=255) cAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGC‑CCCGCATCGAGACCCGCCCGTCGCGCAGCGGc > 5:341392/1‑147 (MQ=255) cAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGA‑CCCGCATCGAGACCCGCCCGTCGCGCAGCGGc > 1:124508/1‑147 (MQ=255) cAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGA‑CCCGCATCGAGACCCGCCCGTCGCGCAGCGGc > 4:52127/1‑147 (MQ=255) cAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGA‑CCCGCATCGAGACCCGCCCGTCGCGCAGCGGc > 1:154700/1‑147 (MQ=255) ggAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGA‑CCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGt < 3:14703/149‑1 (MQ=255) ggAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGA‑CCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGt < 3:52127/149‑1 (MQ=255) aaGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGA‑CCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTgg < 7:230028/149‑1 (MQ=255) gTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGA‑CCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGAc < 2:232054/149‑1 (MQ=255) tACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGTGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGA‑CCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGAc < 2:290292/148‑1 (MQ=255) ccgccgACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGA‑CCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCt > 2:264235/1‑148 (MQ=255) ccGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGA‑CCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGttc < 1:264235/149‑1 (MQ=255) gCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGA‑CCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGttctt > 7:50384/1‑148 (MQ=255) acgacAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGA‑CCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATc < 2:124508/149‑1 (MQ=255) cAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGA‑CCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGAc > 7:13814/1‑129 (MQ=255) cAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGA‑CCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGAc < 8:13814/129‑1 (MQ=255) cAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGA‑CCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACt > 5:173198/1‑149 (MQ=255) aaGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGA‑CCCGCATCGAGACCCGCCCGTCGCGCAGCGGCaa < 5:164987/122‑1 (MQ=255) aaGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGA‑CCCGCATCGAGACCCGCCCGTCGCGCAGCGGCaa > 6:164987/1‑122 (MQ=255) cTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGA‑CCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGa > 2:318385/1‑141 (MQ=255) cTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGA‑CCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGa < 1:318385/141‑1 (MQ=255) tttCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGA‑CCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACcgc > 7:2441/1‑149 (MQ=255) gATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGA‑CCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGAcc > 1:13403/1‑149 (MQ=255) gAGCAACAAGAAAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGTCATCGACCTGA‑CCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCAACGCGACCCGc > 5:74270/1‑149 (MQ=255) gCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGA‑CCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCt < 8:50384/148‑1 (MQ=255) aacaaGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGA‑CCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACgt > 7:154869/1‑107 (MQ=255) aacaaGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGA‑CCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACgt < 8:154869/107‑1 (MQ=255) acaaGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGA‑CCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGAt > 6:273423/1‑148 (MQ=255) acaaGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGA‑CCCGCATCGAGACCCGCCCGTCGCGCAGCCGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGAt > 3:116222/1‑148 (MQ=255) aaGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGAGTCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGAta > 1:99212/1‑147 (MQ=255) gCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAAAGGCATCGAACAGA‑CCCGCATCGAGACCCGCCCGTCGAGCACCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGTCCACCACCGCGACCCGCTGATCAAgg > 4:76097/1‑149 (MQ=255) aGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGA‑CCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACCTCGTCGGCCACCACCGCGACCCGCTGATCAAGGCgg > 7:156715/1‑149 (MQ=255) ttGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGA‑CCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGc < 3:290781/130‑1 (MQ=255) ttGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGA‑CCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGc > 4:290781/1‑130 (MQ=255) cATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGA‑CCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGATCAAGGCGGTGCTCGAg > 6:182511/1‑147 (MQ=255) tGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGA‑CCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGATCAAGGCGGTGCTCGAGCAGa < 1:152619/149‑1 (MQ=255) aGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGA‑CCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGATCAAGGCGGTGCTCGAGCAGAt < 7:82622/148‑1 (MQ=255) ttgagggTGCCGTTCTATCACAACGGCATCGACCTGA‑CCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGATCAAGGCGGTGCTCGAGCAGATCa < 3:172848/143‑1 (MQ=255) gCTGGTGCCGTTCTATCAGAACGGCATCGACCTGA‑CCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGATCAAGGCGGTGCTCGAGCAGATCAGc > 4:93602/1‑148 (MQ=255) gatGCCGTTCTGATAGAACGGCATCGACCTGA‑CCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGATCAAGGCGGTGCTCGAGCAGATCAGCCag > 8:185292/3‑148 (MQ=255) gTGCCGTTCTATCAGAACGGCATCGACCTGA‑CCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGATCAAGGCGGTGCTCGAGCAGATCAGCCagga < 2:154700/149‑1 (MQ=255) gTGCCGTTCTATCAGAACGGCATCGACCTGA‑CCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGATCAAGGCGGTGCTCGAGCAGATCAGCCag > 4:25376/1‑147 (MQ=255) gCCGTTCTATCAGAACGGCATCGACCTGA‑CCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGATCAAGGCGGTGCTCGAGCAGATCAGCCagg > 2:55163/1‑146 (MQ=255) gCCGTTCTATCAGAACGGCATCGACCTGA‑CCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGATCAAGGCGGTGCTCGAGCAGATCAGCCagg < 1:55163/146‑1 (MQ=255) ccGTTCTATCAGAACGGCATCGACCTGA‑CCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGATCAAGGCGGTGCTCGAGCAGATCAGCCAGGAGGc > 7:202219/1‑149 (MQ=255) cTATCAGAACGGCATCGACCTGA‑CCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGATCAAGGCGGTGCTCGAGCAGATCAGCCAGGAGGCtgtg < 2:99212/148‑1 (MQ=255) cTATCAGAACGGCATCGACCTGA‑CCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGATCAAGGCGGTGCTCGAGCAGATCAGCCAGGAGGCtgtg < 4:258717/148‑1 (MQ=255) aTCAGAACGGCATCGACCTGA‑CCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGATCAAGGCGGTGCTCGAGCAGATCAGCCAGGAGGCTGTGgcg > 8:773/1‑149 (MQ=255) aTCAGAACGGCATCGACCTGA‑CCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGATCAAGGCGGTGCTCGAGCAGATCAGCCAGGAGGCTGTGgcg > 4:43561/1‑149 (MQ=255) tCAGAACGTCATCGACCTGA‑CCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGATCAAGGCGGTGCTCGAGCAGATCAGCCAGGAGGCTGTGgcgc < 6:74270/149‑1 (MQ=255) tCAGAACGGCATCGACCTGA‑CCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGATCAAGGCGGTGCTCGAGCAGATCAGCCAGGAGGCTGTGgcg < 8:2441/148‑1 (MQ=255) | GAAGATCGAAGACCGCCCGGACAACTCCACGCGCTTCCTGATGATCGGTAACCAGGAAGTACCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCGATGAGCAACAAGCCAGGTGCGTTGCATGAGTTGCTGGTGCCGTTCTATCAGAACGGCATCGACCTGA‑CCCGCATCGAGACCCGCCCGTCGCGCAGCGGCAAGTGGACCTACGTGTTCTTCATCGACTTCGTCGGCCACCACCGCGACCCGCTGATCAAGGCGGTGCTCGAGCAGATCAGCCAGGAGGCTGTGGCGC > NC_002947/1975839‑1976134 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |