Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,987,443	G→A	10.0%	S233N (AGT→AAT)	PP_1778 →	lipopolysaccharide ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,987,443	0	G	A	10.0%	187.8 / 9.6	70	S233N (AGT→AAT)	PP_1778	lipopolysaccharide ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (33/30);  new base A (4/3);  total (37/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.90e-01

AGGTGTTGTTCTGGTTTACCCCAATCGTTTACATGGTCAATGTGATACCTGAACACCTTCGGGCCACCCTTAGTTTTAACCCCATGTATCCCATTGTTACTGCCTATCACGACGTACTCCTGTACGCTAAAGCGCCTGATTTGAGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTGTTGTTGCTTGGCCTGTTCCTGTTTCGTCGGTCTGTGCCTGAAATGGTGGATGTACTATGAGCCTTTTGAGTGTCAGTGATGTGGGCAAAGCCTACAAAACGTACCGTTCG  >  NC_002947/1987299‑1987590                                                                                                                                                 |                                                                                                                                                     aGGTGTTGTTCTGGTTTACCCCAATCGTTTACATGGTCAATGTGATACCTGAACACCTTCGGGCCACCCTTAGTTTTAACCCCATGTATCCCATTGTTACTGCCTATCACGACGTACTCCTGTACGCTAAAGCGCCTGATTTGAGTCAg                                                                                                                                                  >  4:65557/1‑149 (MQ=255)      ttgttCTGGTTTACCCCAATCGTTTACATGGTCAATGTGATACCTGAACACCTTCGGGCCACCCTTAGTTTTAACCCCATGTATCCCATTGTTACTGCCTATCACGACGTACTCCTGTACGCTAAAGCGCCTGACTCAAATCAGGCgc                                                                                                                                              <  8:70049/148‑2 (MQ=255)        gttCTGGTTTACCCCAATCGTTTACATGGTAAATGTGATACCTTAACACCTTCGGGCCACCCTTAGTTTTAACCCCATGTATCCCATTGTTACTGCCTATCACGACGTACTCCTGTACGCTAAAGCGCCTGATTTGAGTCAGGCGGtt                                                                                                                                            <  8:51391/148‑1 (MQ=255)         ttCTGGTTTACCCCAATCGTTTACATGGTCAATGTGATACCTGAACACCTTCGGGCCACCCTTAGTTTTAACCCCATGTATCCCATTGTTACTTCCTATCACGACGTACTCCTGTACGCTAAAGCGCCTGATTTGAGTCAGGCGGTTGt                                                                                                                                          <  8:48843/149‑1 (MQ=255)           cTGGTTTACCCCAATCGTTTACATGGTCAATGTGATACCTGAACACCTTCGGGCCACCCTTAGTTTTAACCCCATGTATCCCATTGTTACTGCCTATCACGACGTACTCCTGTACGCTAAAGCGCCTGATTTGAGTCAGGCGGTTGTc                                                                                                                                         <  4:312427/148‑1 (MQ=255)              gTTTACCCCAATCGTTTACATGGTCAATGTGATACCTGAACACCTTCGGGCCACCCTTAGTTTTAACCCCATGTATCCCATTGTTACTGCCTATCACGACGTACTCCTGTACGCTAAAGCGCCTGATTTGAGTCAGGCGGTTGTCAt                                                                                                                                       <  2:289556/147‑1 (MQ=255)                    cccAATCGTTTACATGGTCAATGTGATACCTGAACACCTTCGGGCCACCCTTAGTTTTAACCCCATGTATCCCATTGTTACTGCCTATCACGACGTACTCCTGTACGCTAAAGCGCCTGATTTGAGTCAGTCGGTTGTCATGACCGGGa                                                                                                                               >  1:11257/1‑149 (MQ=255)                    cccAATCGTTTACATGGTCAATGTGATACCTGAACACCTTCGGGCCACCCTTAGTTTTAACCCCATGTATCCCATTGTTACTGCCTATCACGACGTACTCCTGTACGCTAAAGCGCCTGATTTGAGTCAGGCGGTTGTCATGACCGGGa                                                                                                                               >  2:267553/1‑149 (MQ=255)                           gTTTACATGGTCAATGTGATACCTGAACACCTTCGGGCCACCCTTAGTTTTAACCCCATGTATCCCATTGTTACTGCCTATCACGACGTACTCCTGTACGCTAAAGCGCCTGATTTGAGTCAGGCGGTTGTCATGACCGGGATTTCCtt                                                                                                                        <  1:168529/149‑1 (MQ=255)                              tACATGGTCAATGTGATACCTGAACACCTTCGGGCCACCCTTAGTTTTAACCCCATGTATCCCATTGTTACTGCCTATCACGACGTACTCCTGTACGCTAAAGCGCCTGATTTGAGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑g                                                                                                                     <  2:11257/148‑1 (MQ=255)                               aCATGGTCAATGTGATACCTGAACACCTTCGGGCCACCCTTAGTTTTAACCCCATGTATCCCATTGTTACTGCCTATCACGACGTACTCCTGTACGCTAAAGCGCCTGATTTGAGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGt                                                                                                                   >  1:188724/1‑149 (MQ=255)                                  tGGTCAATGTGATACCTGAACACCTTCGGGCCACCCTTAGTTTTAACCCCATGTATCCCATTGTTACTGCCTATCACGACGTACTCCTGTACGCTAAAGCGCCTGATTTGAGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCtg                                                                                                                >  4:176502/1‑149 (MQ=255)                                     tCAATGTGATACCTGAACACCTTCGGGCCACCCTTAGTTTTAACCCCATGTATCCCATTGTTACTGCCTATCACGACGTACTCCTGTACGCTAAAGCGCCTGATTTGAGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCtgttg                                                                                                             >  5:82947/1‑149 (MQ=255)                                       aaTGTGATACCTGAACACCTTCGGGCCACCCTTAGTTTTAACCCCATGTATCCCATTGTTACTGCCTATCACGACGTACTCCTGTACGCTAAAGCGCCTGATTTGAGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTgttgtt                                                                                                           <  2:191585/149‑1 (MQ=255)                                         tgtgATACCTGAACACCTTCGGGCCACCCTTAGTTTTAACCCCATGTATCCCATTGTTACTGCCTATCACGACGTACTCCTGTACGCTAAAGCGCCTGATTTGAGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTGTTGTTGc                                                                                                         >  8:38748/1‑149 (MQ=255)                                                ccTGAACACCTTCGGGCCACCCTTAGTTTTAACCCCATGTATCCCATTGTTACTGCCTATCACGACGTACTCCTGTACGCTAAAGCGCCTGATTTGAGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTGTTGTTGCTTGGcct                                                                                                  >  3:122026/1‑149 (MQ=255)                                                       acCTTCGGGCCACCCTTAGTTTTAACCCCATGTATCCCATTGTTACTGCCTATCACGACGTACTCCTGTACGCTAAAGCGCCTGATTTGAGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTGTTGTTGCTTGGcctgttcctg                                                                                           >  4:53773/1‑149 (MQ=255)                                                                    ccTTAGTTTTAACCCCATGTATCCCATTGTTACTGCCTATCACGACGTACTCCTGTACGCTAAAGCGCCTGATTTGAGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTGTTGTTGCTTg                                                                                                      >  8:28205/1‑125 (MQ=255)                                                                    ccTTAGTTTTAACCCCATGTATCCCATTGTTACTGCCTATCACGACGTACTCCTGTACGCTAAAGCGCCTGATTTGAGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTGTTGTTGCTTg                                                                                                      <  7:28205/125‑1 (MQ=255)                                                                     cTTAGTTTTAACCCCATGTATCCCATTGTTACTGCCTATCACGACGTACTCCTGTACGCTAAAGCGCCTGATTTGAGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTGTTGTTGCTTGGCCTGTTCCTGTTTCGTCGGTCtgt                                                                             <  2:143757/149‑1 (MQ=255)                                                                      ttAGTTTTAACCCCATGTATCCCATTGTTACTGCCTATCACGACGTACTCCTGTACGCTAAAGCGCCTGACTCAAATCAGGCGCtt                                                                                                                                            >  1:290543/1‑86 (MQ=38)                                                                      ttAGTTTTAACCCCATGTATCCCATTGTTACTGCCTATCACGACGTACTCCTGTACGCTAAAGCGCCTGACTCAAATCAGGCGCtt                                                                                                                                            <  2:290543/86‑1 (MQ=38)                                                                        aGTTTTAACCCCATGTATCCCATTGTTACTGCCTATCACGACGTACTCCTGTACGCTAAAGCGCCTGATTTGAGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTGTTGTTGCTTGGCCTGTTCCTGTTTCGTCGGTCTGTGc                                                                           <  6:82947/148‑1 (MQ=255)                                                                          ttttAACCCCATGTATCCCATTGTTACTGCCTATCACGACGTACTCCTGTACGCTAAAGCGCCTGATTTGAGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTGTTGTTGCTTGGCCTGTTCCTGTTTCGTCGGTCTGTGCCt                                                                         >  1:219205/1‑148 (MQ=255)                                                                          ttttAACCCCATGTATCCCATTGTTACTGCCTATCACGACGTACTCCTGTACGCTAAAGCGCCTGATTTGAGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTGTTGTTGCTTGGCCTGTTCCTGTTTCGTCGGTCTGTGCCt                                                                         >  3:83596/1‑148 (MQ=255)                                                                                ccccATGTATCCCATTGTTACTGCCTATCACGACGTACTCCTGTACGCTAAAGCGCCTGATTTGAGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTGTTGTTGCTTGGCCTGTTCCTGTTTCGTCGGTCTGTGCCTGAAAtgg                                                                  >  6:346984/1‑149 (MQ=255)                                                                                 cccATGTATCCCATTGTTACTGCCTATCACGACGTACTCCTGTACGCTAAAGCGCCTGATTTGAGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTGTTGTTGCTTGGCCTGTTCCTGTTTCGTCGGTCTGTGCCTGAAAtggt                                                                 <  2:219205/149‑1 (MQ=255)                                                                                   cATGTATCCCATTGTTACTGCCTATCACGACGTACTCCTGTACGCTAAAGCGCCTGATTTGAGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTGTTGTTGCTTGGCCTGTTCCTGTTTCGTCGGTCTGTGCCTGAAAtggtt                                                                >  4:265467/1‑147 (MQ=255)                                                                                   cATGTATCCCATTGTTACTGCCTATCACGACGTACTCCTGTACGCTAAAGCGCCTGATTTGAGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTGTTGTTGCTTGGCCTGTTCCTGTTTCGTCGGTCTGTGCCTGAAAtggtg                                                                >  6:26880/1‑148 (MQ=255)                                                                                      gTATCCCATTGTTACTGCCTATCACGACGTACTCCTGTACGCTAAAGCGCCTGATTTGAGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTGTTGTTGCTTGGc                                                                                                    >  3:145496/1‑109 (MQ=255)                                                                                      gTATCCCATTGTTACTGCCTATCACGACGTACTCCTGTACGCTAAAGCGCCTGATTTGAGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTGTTGTTGCTTGGc                                                                                                    <  4:145496/109‑1 (MQ=255)                                                                                      gTATCCCATTGTTACTGCCTATCACGACGTACTCCTGTACGCTAAAGCGCCTGATTTGAGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTGTTGTTGCTTGGCCTGTTCCTGTTTCGTCGGTCTGTGCCTGAAATGGTGGATg                                                            >  7:13731/1‑149 (MQ=255)                                                                                       tATCCATTTGTTACTGCTTATCACGAGGTACTCCTGTACGCTAACGCGCCTGATTTTAGTCAGGCGGTTGTAATGACCGGCATTTCCTTTCGGTCTGTTGTTGCTTGGCCTGTTCCTGTTTCGTCGGTCTGTGCCTGAAATGGTGGAt                                                             <  1:267553/148‑1 (MQ=255)                                                                                           ccATTGTTACTGCCTATCACGACGTACTCCTGTACGCTAAAGCGCCTGATTTGAGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTGTTGTTGCTTGGcctgttcc                                                                                             <  2:8516/111‑1 (MQ=255)                                                                                           ccATTGTTACTGCCTATCACGACGTACTCCTGTACGCTAAAGCGCCTGATTTGAGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTGTTGTTGCTTGGcctgttcc                                                                                             >  1:8516/1‑111 (MQ=255)                                                                                                gTTACTGCCTATCACGACGTACTCCTGTACGCTAAAGCGCCTGATTTGAGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTGTTGTTGCTTGGCCTGTTCCTGTTTCGTCGGTCTGTGCCTGAAATGGTGGATGTACTATGAg                                                   <  8:100336/148‑1 (MQ=255)                                                                                                 ttACTGCCTATCACGACGTACTCCTGTACGCTAAAGCGCCTGATTTGAGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTGTTGTTGCTTGGCCTGTTCCTGTTTCGTCGGTCTGTGCCTGAAATGGTGGATGTACTATGAGcc                                                 >  7:274534/1‑149 (MQ=255)                                                                                                       ccTATCACGACGTACTCCTGTACGCTAAAGCGCCTGATTTGAGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTGTTGTTGCTTGGCCTGTTCCTGTTTCGTCGGTc                                                                                >  8:110957/1‑112 (MQ=255)                                                                                                       ccTATCACGACGTACTCCTGTACGCTAAAGCGCCTGATTTGAGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTGTTGTTGCTTGGCCTGTTCCTGTTTCGTCGGTc                                                                                <  7:110957/112‑1 (MQ=255)                                                                                                       ccTATCACGACGTACTCCTGTACGCTAAAGCGCCTGATTTGAGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTGTTGTTGCTTGGCCTGTTCCTGTTTCGTCGGTCTGTGCCTGAAATGGTGGATGTACTATGAGCCTTTTGa                                           <  4:122026/149‑1 (MQ=255)                                                                                                       ccTATCACGACGTACTCCTGTACGCTAAAGCGCCTGATTTGAGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTGTTGTTGCTTGGCCTGTTCCTGTTTCGTCGGTCTGTGCCTGAAATGGTGGATGTACTATGAGCCTTTTGa                                           <  5:26880/149‑1 (MQ=255)                                                                                                       ccTATCACGACGTACTCCTGTACGCTAAAGCGCCTGATTTGAGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTGTTGTTGCTTGGCCTGTTCCTGTTTCGTCGGTCTGTGCCTGAAATGGTGGATGTACTATGAGCCTTTTGa                                           <  3:53773/149‑1 (MQ=255)                                                                                                        cTATCACGACGTACTCCTGTACGCTAAAGCGCCTGATTTGAGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTGTTGTTGCTTGGCCTGTTCCTGTTTCGTCGGTCTGTGCCTGAAATGGTGGATGTACTATGAGCCTTTTGa                                           <  2:188724/148‑1 (MQ=255)                                                                                                        cTATCACGACGTACTCCTGTACGCTAAAGCGCCTGATTTGAGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTGTTGTTCCTTGGCCTGTGCCTGTTTCGTCGGTCTGTGCCTGAAATGGTGGATGTACTATGAGCCTTTTGAg                                          >  5:116778/1‑149 (MQ=255)                                                                                                          aTCACGACGTACTCCTGTACGCTAAAGCGCCTGATTTGAGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTGTTGTTGCTTGGCCTGTTCCTGTTTCGTCGGTCTGTGGCTGAAATGGTGGATGTACTATGAGCCTTTTGAgtg                                        >  5:76929/1‑149 (MQ=255)                                                                                                             acgacgTACTCCTGTACGCTAAAGCGCCTGATTTGAGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTGTTGTTGCTTGGCCTGTTCCTGTTtc                                                                                       >  1:325884/1‑99 (MQ=255)                                                                                                             acgacgTACTCCTGTACGCTAAAGCGCCTGATTTGAGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTGTTGTTGCTTGGCCTGTTCCTGTTtc                                                                                       <  2:325884/99‑1 (MQ=255)                                                                                                                  gTACTCCTGTACGCTAAAGCGCCTGATTTGAGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTGTTGTTGCTTGGCCTGTTCCTGTTTCGTCGGTCTGTGCCTGAAATGGTGGATGTACTATGAGCCTTTTGAGTGTCAGTGAt                                <  4:83596/149‑1 (MQ=255)                                                                                                                  gTACTCCTGTACGCTAAAGCGCCTGATTTGAGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTGTTGTTGCTTGGCCTGTTCCTGTTTCGTCGGTCTGTGCCTGAAATGGTGGATGTACTATGAGCCTTTTGAGTGTCAGTGAt                                <  8:274534/149‑1 (MQ=255)                                                                                                                    aCTCCTGTACGCTAAAGCGCCTGATTTGAGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTGTTGTTGCTTGGCCTGTTCCTGTTTCGTCGGTCTGTGCCTGAAATGGTGGATGTACTATGAGCCTTTTGAGTGTCAGTGAtgt                              >  5:221115/1‑149 (MQ=255)                                                                                                                       ccTGTACGCTAAAGCGCCTGATTTGAGTCAGGCGGTTGTCTTGAACGGGATTTCCTTT‑CGTCTGTTGTTGCTTGGCCTGTTCCTGTTTCTTAGGTCTGTGCCTGAAATGGTGGATGTACTATGAGCCTTATGAGTGTCAGTGATGTggg                           >  3:177202/1‑149 (MQ=255)                                                                                                                        cTGTACGCTAAAGCGCCTGATTTGAGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTGTTGTTGCTTGGCCTGTTCCTGTTTCGTCGGTCTGTGCCTGAAATGGTGGATGTACTATGAGCCTTTTGAGTGTCAGTGATGTggg                           >  5:119590/1‑148 (MQ=255)                                                                                                                        cTGTACGCTAAAGCGCCTGATTTGAGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTGTTGTTGCTTGGCCTGTTCCTGTTTCGTCGGTCTGTGCCTGAAATGGTGGATGTACTATGAGCCTTTTGAGTGTCAGTGATGTggg                           >  2:14603/1‑148 (MQ=255)                                                                                                                           tACGCTAAAGCGCCTGATTTGAGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTGTTGTTGCTTGGcctgttcct                                                                                            <  8:62784/80‑1 (MQ=255)                                                                                                                           tACGCTAAAGCGCCTGATTTGAGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTGTTGTTGCTTGGcctgttcct                                                                                            >  7:62784/1‑80 (MQ=255)                                                                                                                           tACGCTAAAGCGCCTGATTTGAGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTGTTGTTGCTTGGCCTGTTCCTGTTTCGTCGGTCTGTGCCTGAAATGGTGGATGTACTATGAGCCTTTTGAGTGTCAGTGATGTGGGCa                         >  7:102131/1‑147 (MQ=255)                                                                                                                             cGCTAAAGCGCCTGATTTGAGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTGTTGTTGCTTGGCCTGTTCCTGTTTCGTCGGTCTGTGCCTGAAATGGTGGATGTACTATGAGCCTTTTGAGTGTCAGTGATGTGGGCAAAGc                     <  3:65557/149‑1 (MQ=255)                                                                                                                              gCTAAAGCGCCTGATTTGAGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTGTTGTTGCTTGGCCTGTTCCTGTTTCGTCGGTCTGTGCCTGAAATGGTGGATGTACTATGAGCCTTTTGAGTGTCAGTGATGTGGGCAAAGcc                    <  6:221115/149‑1 (MQ=255)                                                                                                                                tAAAGCGCCTGATTTGAGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTGTTGTTGCTTGGCCTGTTCCTGTTTCGTCGGTCTGTGCCTGAAATGGTGGATGTACTATGAGCCTTTTGAGTGTCAGTGATGTGGGCAAAGCCTa                  >  8:45939/1‑149 (MQ=255)                                                                                                                                   accgcCTGACTCAAATCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTGTTGTTGCTTGGCCTGTTCCTGTTTCGTCGGTCTGTGCCTGAAATGGTGGATGTACTATGAGCCtttt                                             <  3:268817/117‑1 (MQ=255)                                                                                                                                   accgcCTGACTCAAATCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTGTTGTTGCTTGGCCTGTTCCTGTTTCGTCGGTCTGTGCCTGAAATGGTGGATGTACTATGAGCCtttt                                             >  4:268817/3‑119 (MQ=255)                                                                                                                                    ccgcCTGACTCAAATCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTGTTGTTGCTTGGCCTGTTCCTGTTTCGTCGGTCTGTGCCTGAAATGGTGGATGTACTATGAGCCTTTTGAGTGTCAGTGATGTGGGCAAAGCCTACaa               >  2:164178/2‑148 (MQ=255)                                                                                                                                     cgcCTGACTCAAATCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTGTTGTTGCTTGGCCTGTTCCTGTTTCGTCGGTCTGTGCCTGAAATGGTGGATGTACTATGAGCCTTTTGAGTGTCAGTGATGTGGGCAAAGCCTACaaaa             >  6:192536/1‑149 (MQ=255)                                                                                                                                      gcCTGATTTGAGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTGTTGTTGCTTGGCCTGTTCCTGTTTCGTCGGTCTGTGCCTGAAATGGTGGATGTACTATGAGCCTTTTGAGTGTCAGTGATGTGGGCAAAGCCTACAAAAc            >  1:25933/1‑149 (MQ=255)                                                                                                                                        cTGATTTGAGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTGTTGTTGCTTGGCCTGTTCCTGTTTCGTCGGTCTGTGCCTGAAATGGTGGATGTACTATGAGCCTTTTGAGTGTCAGTGATGTGGGCAAAGCCTACAAAACGt          <  8:102131/149‑1 (MQ=255)                                                                                                                                           aTTTGAGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTGTTGTTGCTTGGCCTGTTCCTGTTTCGTCGGTCTGTGCCTGAAATGGTGGATGTACTATGAGCCTTTTGAGTGTCAGTGATGTGGGCAAAGCCTACAAAACGTa         <  3:176502/147‑1 (MQ=255)                                                                                                                                           aTTTGAGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTGTTGTTGCTTGGCCTGTTCCTGTTTCGTCGGTCTGTGCCTGAAATGGTGGATGTACTATGAGCCTTTTGAGTGTCAGTGATGTGGGCAAAGCCTACAAAACGTAc        <  6:76929/148‑1 (MQ=255)                                                                                                                                               gAGTCAGGCGGTTGTTATGACCGGGATTTCCTTT‑GGTCTGTTGTTGCTTGGCCTGTTCCTGTTTCGTCGGTCTGTGCCTGAAATGGTGGATGTACTATGAGCCTTTTGAGTGTCAGTGATGTGGGCAAAGCCTACAAAACGTACCGTTc   >  2:154068/1‑149 (MQ=255)                                                                                                                                                aGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTGTTGTTGCTTGGCCTGTTCCTGTTTCGTCGGTCTGTGCCTGAAATGGTGGATGTACTATGAGCCTTTTGAGTGTCAGTGATGTGGGCAAAGCCTACAAAACGTa         <  6:333772/142‑1 (MQ=255)                                                                                                                                                aGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTGTTGTTGCTTGGCCTGTTCCTGTTTCGTCGGTCTGTGCCTGAAATGGTGGATGTACTATGAGCCTTTTGAGTGTCAGTGATGTGGGCAAAGCCTACAAAACGTACAGATCg  >  5:333772/1‑149 (MQ=255)                                                                                                                                                 |                                                                                                                                                     AGGTGTTGTTCTGGTTTACCCCAATCGTTTACATGGTCAATGTGATACCTGAACACCTTCGGGCCACCCTTAGTTTTAACCCCATGTATCCCATTGTTACTGCCTATCACGACGTACTCCTGTACGCTAAAGCGCCTGATTTGAGTCAGGCGGTTGTCATGACCGGGATTTCCTTT‑GGTCTGTTGTTGCTTGGCCTGTTCCTGTTTCGTCGGTCTGTGCCTGAAATGGTGGATGTACTATGAGCCTTTTGAGTGTCAGTGATGTGGGCAAAGCCTACAAAACGTACCGTTCG  >  NC_002947/1987299‑1987590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑