Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,010,459 T→A 11.7% E325D (GAA→GAT) ‡ PP_1792 ← group 2 family glycosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,010,4590TA11.7% 126.5 / 7.0 50E325D (GAA→GAT) ‡PP_1792group 2 family glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base T (22/22);  new base A (3/3);  total (25/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCTTGTGGCCCGTCGCGGTCACAGTCGGCATAAGCCCAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGCGTCACCTTCACTTAGCAGTAACAGGCCGGGTCTGAACCTGGAT  >  NC_002947/2010312‑2010603
                                                                                                                                                   |                                                                                                                                                
gCCTTGTGGCCCGTCGCGGTCACAGTCGGCATAAGCCCAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAgttgt                                                                                                                                                  <  6:235898/148‑1 (MQ=255)
gCCTTGTGGCCCGTCGCGGTCACAGTCGGCATAAGCCCAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAgttgt                                                                                                                                                  <  7:145640/148‑1 (MQ=255)
   ttGTGGCCCGTCGCGGTCACAGTCGGCATAAGCCCAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCaa                                                                                                                                              >  1:241172/1‑149 (MQ=255)
     gtgGCCCGTCGCGGTCACAGTCGGCATAAGCCCAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCaa                                                                                                                                              <  7:142261/147‑1 (MQ=255)
        gCCCGTCGCGGTCACAGTCGGCATAAGCCCAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCg                                                                                                                                         >  7:169421/1‑149 (MQ=255)
          ccGTCGCGGTCACAGTCGGCATAAGCCCAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCggg                                                                                                                                       <  2:114998/149‑1 (MQ=255)
             tCGCGGTCACAGTCGGCATAAGCCCAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGgag                                                                                                                                    <  7:221385/149‑1 (MQ=255)
                  gTCACAGTCGGCATAAGCCCAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGt                                                                                                                               <  5:20722/149‑1 (MQ=255)
                        gTCGGCATAAGCCCAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCg                                                                                                                         <  1:240599/149‑1 (MQ=255)
                         tCGGCATAAGCCCAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCgg                                                                                                                        >  1:171822/1‑149 (MQ=255)
                              aTAAGCCCAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGCTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCgg                                                                                                                                        >  2:43528/1‑128 (MQ=255)
                              aTAAGCCCAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGCTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCgg                                                                                                                                        <  1:43528/128‑1 (MQ=255)
                                   cccAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGAACAACTGCtgg                                                                                                                                       >  3:203602/1‑124 (MQ=255)
                                   cccAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGAACAACTGCtgg                                                                                                                                       <  4:203602/124‑1 (MQ=255)
                                    ccAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCg                                                                                                             >  8:205142/1‑149 (MQ=255)
                                     cAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGc                                                                                                            >  3:141714/1‑149 (MQ=255)
                                               cTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATgg                                                                                                  <  8:169421/149‑1 (MQ=255)
                                                    tccAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGGGATCTGTATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCa                                                                                                          <  4:287600/136‑1 (MQ=255)
                                                    tccAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCa                                                                                                          >  3:287600/1‑136 (MQ=255)
                                                    tccAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACt                                                                                             >  7:238625/1‑149 (MQ=255)
                                                                 gAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCg                                                                                >  3:133896/1‑149 (MQ=255)
                                                                 gAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCg                                                                                <  7:292376/149‑1 (MQ=255)
                                                                    gCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCCGCAGTTGAACAACTg                                                                                                                                           <  5:232074/87‑1 (MQ=255)
                                                                    gCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCCGCAGTTGAACAACTg                                                                                                                                           >  6:232074/1‑87 (MQ=255)
                                                                     cTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCa                                                                            <  7:205142/149‑1 (MQ=255)
                                                                        cgcCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAgg                                                                          <  2:241172/148‑1 (MQ=255)
                                                                         gcCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGgaga                                                                                                                                   >  5:31888/1‑90 (MQ=255)
                                                                         gcCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGgaga                                                                                                                                   <  6:31888/90‑1 (MQ=255)
                                                                               gCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGa                                                                   <  3:176182/148‑1 (MQ=255)
                                                                                      gcgcCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGAtt                                                           <  8:238625/149‑1 (MQ=255)
                                                                                        gcCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGAtttt                                                         <  2:171822/149‑1 (MQ=255)
                                                                                         ccAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGAttttt                                                        >  6:86744/1‑149 (MQ=255)
                                                                                          cAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGAtttttt                                                       >  8:279414/1‑149 (MQ=255)
                                                                                          cAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGAtttttt                                                       >  2:330621/1‑149 (MQ=255)
                                                                                           aGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTc                                                      <  7:279414/149‑1 (MQ=255)
                                                                                                   aCCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGTGTgg                                              >  5:178309/1‑149 (MQ=255)
                                                                                                   aCCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTg                                               <  1:330621/148‑1 (MQ=255)
                                                                                                    ccTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGc                                             >  5:252336/1‑149 (MQ=255)
                                                                                                                 ggATAAAGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACAGCGGGGAGAAGGTCGGCCGCGGCAGCCTTCTCCAGTTGATGGACCCTTTCAGCGTCATCGCCCAGGCAATCGAGGCTGATTTTTTCGATAGTGGCGTCACCTTCACt                                 >  7:127329/1‑148 (MQ=255)
                                                                                                                  gATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGCGTCACCTTCACt                                 >  3:70598/1‑147 (MQ=255)
                                                                                                                            tCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGTCGTCACCTTCACTTAGCAGTAACAg                     >  4:222990/1‑149 (MQ=255)
                                                                                                                                aGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGCGTCACCTTCACTTAGCAGTAACAGGCCg                 >  7:49158/1‑149 (MQ=255)
                                                                                                                                aGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGCGTCACCTTCACTTAGCAGTAACAGGCCg                 >  7:51028/1‑149 (MQ=255)
                                                                                                                                aGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGCGTCACCTTCACTTAGCAGTAACAGGCCg                 <  4:70598/149‑1 (MQ=255)
                                                                                                                                 gCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGTGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGCGTCACCTTCACTTAGCAGTAACAGGCCgg                <  5:86744/149‑1 (MQ=255)
                                                                                                                                  ccGGCTGCCAGCAGATGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGCGTCACCTTCACTTAGCAGTAACAGGCCggg               >  8:200956/1‑149 (MQ=255)
                                                                                                                                     gCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTGCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGCGTCACCTTCACTTAGCAGTAACAGGCCGGGGCt            <  4:141714/149‑1 (MQ=255)
                                                                                                                                       tcccAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGCGTCACCTTCACTTAGc                             <  7:74702/128‑1 (MQ=255)
                                                                                                                                       tcccAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGCGTCACCTTCACTTAGc                             >  8:74702/3‑130 (MQ=255)
                                                                                                                                       tGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGCGTCACCTTCACTTAGCAGTAACAGGCCGGGTCTGa          <  6:252336/149‑1 (MQ=255)
                                                                                                                                          ccgcagTTGAACAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCTCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGCGTCACCTTCACTTAGCAGTAACAg                     <  7:187271/133‑1 (MQ=255)
                                                                                                                                          ccgcagTTGAACAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCACCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGCGTCACCTTCACTTAGCAGTAACAg                     >  8:187271/3‑135 (MQ=255)
                                                                                                                                               gttgttCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCCCCGTCATCGACCATGCTATCGAGGCTGATTTTTTCGAGAGTGGCGTCACCTTCACTTAGCAGTAACAGGCGGTGTCTGAAGCTggat  <  4:49067/149‑1 (MQ=255)
                                                                                                                                               gttgttCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGCGTCACCTTCACTTAGCa                            >  8:76949/1‑123 (MQ=255)
                                                                                                                                               gttgttCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGCGTCACCTTCACTTAGCa                            <  7:76949/123‑1 (MQ=255)
                                                                                                                                               gttgttCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGCGTCACCTTCACTTAGCAGTAACAGGCCGGGTCTGAACCTggat  <  7:200956/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
GCCTTGTGGCCCGTCGCGGTCACAGTCGGCATAAGCCCAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGCGTCACCTTCACTTAGCAGTAACAGGCCGGGTCTGAACCTGGAT  >  NC_002947/2010312‑2010603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑