Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,010,460 T→A 12.0% E325V (GAA→GTA) ‡ PP_1792 ← group 2 family glycosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,010,4600TA12.0% 129.7 / 9.0 50E325V (GAA→GTA) ‡PP_1792group 2 family glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base T (22/22);  new base A (3/3);  total (25/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.65e-01

TTGTGGCCCGTCGCGGTCACAGTCGGCATAAGCCCAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGCGTCACCTTCACTTAGCAGTAACAGGCCGGGTCTGAACCTGGATGGGAT  >  NC_002947/2010315‑2010608
                                                                                                                                                 |                                                                                                                                                    
ttGTGGCCCGTCGCGGTCACAGTCGGCATAAGCCCAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCaa                                                                                                                                                   >  1:241172/1‑149 (MQ=255)
  gtgGCCCGTCGCGGTCACAGTCGGCATAAGCCCAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCaa                                                                                                                                                   <  7:142261/147‑1 (MQ=255)
     gCCCGTCGCGGTCACAGTCGGCATAAGCCCAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCg                                                                                                                                              >  7:169421/1‑149 (MQ=255)
       ccGTCGCGGTCACAGTCGGCATAAGCCCAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCggg                                                                                                                                            <  2:114998/149‑1 (MQ=255)
          tCGCGGTCACAGTCGGCATAAGCCCAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGgag                                                                                                                                         <  7:221385/149‑1 (MQ=255)
               gTCACAGTCGGCATAAGCCCAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGt                                                                                                                                    <  5:20722/149‑1 (MQ=255)
                     gTCGGCATAAGCCCAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCg                                                                                                                              <  1:240599/149‑1 (MQ=255)
                      tCGGCATAAGCCCAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCgg                                                                                                                             >  1:171822/1‑149 (MQ=255)
                           aTAAGCCCAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGCTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCgg                                                                                                                                             >  2:43528/1‑128 (MQ=255)
                           aTAAGCCCAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGCTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCgg                                                                                                                                             <  1:43528/128‑1 (MQ=255)
                                cccAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGAACAACTGCtgg                                                                                                                                            >  3:203602/1‑124 (MQ=255)
                                cccAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGAACAACTGCtgg                                                                                                                                            <  4:203602/124‑1 (MQ=255)
                                 ccAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCg                                                                                                                  >  8:205142/1‑149 (MQ=255)
                                  cAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGc                                                                                                                 >  3:141714/1‑149 (MQ=255)
                                            cTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATgg                                                                                                       <  8:169421/149‑1 (MQ=255)
                                                 tccAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGGGATCTGTATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCa                                                                                                               <  4:287600/136‑1 (MQ=255)
                                                 tccAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCa                                                                                                               >  3:287600/1‑136 (MQ=255)
                                                 tccAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACt                                                                                                  >  7:238625/1‑149 (MQ=255)
                                                              gAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCg                                                                                     <  7:292376/149‑1 (MQ=255)
                                                              gAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCg                                                                                     >  3:133896/1‑149 (MQ=255)
                                                                 gCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCCGCAGTTGAACAACTg                                                                                                                                                <  5:232074/87‑1 (MQ=255)
                                                                 gCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCCGCAGTTGAACAACTg                                                                                                                                                >  6:232074/1‑87 (MQ=255)
                                                                  cTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCa                                                                                 <  7:205142/149‑1 (MQ=255)
                                                                     cgcCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAgg                                                                               <  2:241172/148‑1 (MQ=255)
                                                                      gcCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGgaga                                                                                                                                        <  6:31888/90‑1 (MQ=255)
                                                                      gcCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGgaga                                                                                                                                        >  5:31888/1‑90 (MQ=255)
                                                                            gCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGa                                                                        <  3:176182/148‑1 (MQ=255)
                                                                                   gcgcCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGAtt                                                                <  8:238625/149‑1 (MQ=255)
                                                                                     gcCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGAtttt                                                              <  2:171822/149‑1 (MQ=255)
                                                                                      ccAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGAttttt                                                             >  6:86744/1‑149 (MQ=255)
                                                                                       cAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGAtttttt                                                            >  8:279414/1‑149 (MQ=255)
                                                                                       cAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGAtttttt                                                            >  2:330621/1‑149 (MQ=255)
                                                                                        aGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTc                                                           <  7:279414/149‑1 (MQ=255)
                                                                                                aCCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGTGTgg                                                   >  5:178309/1‑149 (MQ=255)
                                                                                                aCCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTg                                                    <  1:330621/148‑1 (MQ=255)
                                                                                                 ccTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGc                                                  >  5:252336/1‑149 (MQ=255)
                                                                                                              ggATAAAGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACAGCGGGGAGAAGGTCGGCCGCGGCAGCCTTCTCCAGTTGATGGACCCTTTCAGCGTCATCGCCCAGGCAATCGAGGCTGATTTTTTCGATAGTGGCGTCACCTTCACt                                      >  7:127329/1‑148 (MQ=255)
                                                                                                               gATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGCGTCACCTTCACt                                      >  3:70598/1‑147 (MQ=255)
                                                                                                                         tCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGTCGTCACCTTCACTTAGCAGTAACAg                          >  4:222990/1‑149 (MQ=255)
                                                                                                                             aGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGCGTCACCTTCACTTAGCAGTAACAGGCCg                      >  7:49158/1‑149 (MQ=255)
                                                                                                                             aGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGCGTCACCTTCACTTAGCAGTAACAGGCCg                      >  7:51028/1‑149 (MQ=255)
                                                                                                                             aGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGCGTCACCTTCACTTAGCAGTAACAGGCCg                      <  4:70598/149‑1 (MQ=255)
                                                                                                                              gCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGTGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGCGTCACCTTCACTTAGCAGTAACAGGCCgg                     <  5:86744/149‑1 (MQ=255)
                                                                                                                               ccGGCTGCCAGCAGATGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGCGTCACCTTCACTTAGCAGTAACAGGCCggg                    >  8:200956/1‑149 (MQ=255)
                                                                                                                                  gCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTGCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGCGTCACCTTCACTTAGCAGTAACAGGCCGGGGCt                 <  4:141714/149‑1 (MQ=255)
                                                                                                                                    tcccAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGCGTCACCTTCACTTAGc                                  >  8:74702/3‑130 (MQ=255)
                                                                                                                                    tcccAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGCGTCACCTTCACTTAGc                                  <  7:74702/128‑1 (MQ=255)
                                                                                                                                    tGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGCGTCACCTTCACTTAGCAGTAACAGGCCGGGTCTGa               <  6:252336/149‑1 (MQ=255)
                                                                                                                                       ccgcagTTGAACAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCTCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGCGTCACCTTCACTTAGCAGTAACAg                          <  7:187271/133‑1 (MQ=255)
                                                                                                                                       ccgcagTTGAACAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCACCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGCGTCACCTTCACTTAGCAGTAACAg                          >  8:187271/3‑135 (MQ=255)
                                                                                                                                            gttgttCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCCCCGTCATCGACCATGCTATCGAGGCTGATTTTTTCGAGAGTGGCGTCACCTTCACTTAGCAGTAACAGGCGGTGTCTGAAGCTggat       <  4:49067/149‑1 (MQ=255)
                                                                                                                                            gttgttCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGCGTCACCTTCACTTAGCa                                 >  8:76949/1‑123 (MQ=255)
                                                                                                                                            gttgttCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGCGTCACCTTCACTTAGCa                                 <  7:76949/123‑1 (MQ=255)
                                                                                                                                            gttgttCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGCGTCACCTTCACTTAGCAGTAACAGGCCGGGTCTGAACCTggat       <  7:200956/149‑1 (MQ=255)
                                                                                                                                                 tCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGCGTCACCTTCACTTAGCAGTAACAGGCCGGGTCTGAACCTggatgggat  >  3:119953/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
TTGTGGCCCGTCGCGGTCACAGTCGGCATAAGCCCAAGCGCAACCTTCCTCCAGCAAATTGCGAAGCTGCGCCATGGCTCGGGGCGCCAGTTTGTCACCTGCAGCGATCTGGATAATGCTGTCGCAGCCGGCTGCCAGCAGTTGTTCAACTGCGGGGAGAAGGTCGGCCGGGGCAGCCTTCGCCAGTTGATGGACACTTTCAGCGTCATCGCCCAGGCTATCGAGGCTGATTTTTTCGAGAGTGGCGTCACCTTCACTTAGCAGTAACAGGCCGGGTCTGAACCTGGATGGGAT  >  NC_002947/2010315‑2010608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑