Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,039,430 T→A 6.9% K17M (AAG→ATG)  rffE ← UDP‑N‑acetylglucosamine‑2‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,039,4300TA 6.9% 176.5 / 2.3 58K17M (AAG→ATG) rffEUDP‑N‑acetylglucosamine‑2‑epimerase
Reads supporting (aligned to +/- strand):  ref base T (24/30);  new base A (2/2);  total (26/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

GCCGGCTTTCATGATGTTCAAGTCGTATTCGGGGCTGATGCCGAACAGCTCCAGTACCTGATCGAGCATTTGCCGGTGCTGGCCGGTGACGCACACGCGGGCTTCGAAGCGGCTGTCCTGCGCCAGTTGCAGGGCTAGCGGGGCCATCTTGATGGCTTCTGGGCGGGTACCGAAAACAGTCAGGGTCTTGAGGGGCACGGCAGTGATCCTCTTGCTCGGGTCCTTGAAGCAGGCCGGTTTCCATTGCTCAAAAATCTAGCAGGCGGGCAATGGCGTGCCAGTTTGTCGGGGGG  >  NC_002947/2039282‑2039574
                                                                                                                                                    |                                                                                                                                                
gCCGGCTTTCATGATGTTCAAGTCGTATTCGGGGCTGATGCCGAACAGCTCCAGTACCTGATCGAGCATTTGCCGGTGCTGGCCGGTGACGCACACGCGGGCTTCGAAGCGGCTGTCCTGCGCCAGTTGCAGGGCTAGCGGGGCCATCt                                                                                                                                                  >  5:174451/1‑149 (MQ=255)
   ggCTTTCATGATGTTCAAGTCGTATTCGGGGCTGATGCCGAACAGCTCCAGTACCTGATCGAGCATTTGCCGGTGCTGGCCGGTGACGCACACGCGGGCTTCGAAGCGGCTGTCCTGCGCCAGTTGCAGGGCTAGCGGGGCCATCTTGa                                                                                                                                               <  5:109392/149‑1 (MQ=255)
     cTTTCATGATGTTCAAGTCGTATTCGGGGCTGATGCCGAACAGCTCCAGTACCTGATCGAGCATTTGCCGGTGCTGGCCGGTGACGCACACGCGGGCTTCGAAGCGGCTGTCCTGCGCCAGTTGCAGGGCTAGCGGGGCCATCTTGAt                                                                                                                                              <  5:109043/148‑1 (MQ=255)
     cTTTCATGATGTTCAAGTCGTATTCGGGGCTGATGCCGAACAGCTCCAGTACCTGATCGAGCATTTGCCGGTGCTGGCCGGTGACGCACACGCGGGCTTCGAAGCGGCTGTCCTGCGCCAGTTGCAGGGCTAGCGGGGCCATCTTGATg                                                                                                                                             >  2:291997/1‑149 (MQ=255)
        tCATGATGTTCAAGTCGTATTCGGGGCTGATGCCGAACAGCTCCAGTACCTGATCGAGCATTTGCCGGTGCTGGCCGGTGACGCACACGCGGGCTTCGAAGCGGCTGTCCTGCGCCAGTTGCAGGGCTAGCGGGGCCATCTTGAt                                                                                                                                              >  6:109043/1‑145 (MQ=255)
        tCATGATGTTCAAGTCGTATTCGGGGCTGATGCCGAACAGCTCCAGTACCTGATCGAGCATTTGCCGGTGCTGGCCGGTGACGCACACGCGGGCTTCGAAGCGGCTGTCCTGCGCCAGTTGCAGGGCTAGCGGGGCCATCTTGATGGc                                                                                                                                           <  2:213495/148‑1 (MQ=255)
        tCATGATGTTCAAGTCGTATTCGGGGCTGATGCCGAACAGCTCCAGTACCTGATCGAGCATTTGCCGGTGCTGGCCGGTGACGCACACGCGGGCTTCGAAGCGGCTGTCCTGCGCCAGTTGCAGGGCTAGCGGGGCCATCTTGATGGCt                                                                                                                                          >  4:213289/1‑149 (MQ=255)
           tgatgTTCAAGTCGTATTCGGGGCTGATGCCGAACAGCTCCAGTACCTGATCGAGCATTTGCCGGTGCTGGCCGGTGACGCACACGCGGGCTTCGAAGCGGCTGTCCTGAGCCAGTTGCAGGGCTAGCGGGGCCATCTTGATGGCTTCt                                                                                                                                       >  1:159125/1‑149 (MQ=255)
              tgTTCAAGTCGTATTCGGGGCTGATGCCGAACAGCTCCAGTACCTGATCGAGCATTTGCCGGTGCTGGCCGGTGACGCACACGCGGGCTTCGAAGCGGCTGTCCTGCGCCAGTTGCAGGGCTAGCGGGGCCATCTTGATGGCTTCTggg                                                                                                                                    >  8:97020/1‑149 (MQ=255)
                ttCAAGTCGTATTCGGGGCTGATGCCGAACAGCTCCAGTACCTGATCGAGCATTTGCCGGTGCTGGCCGGTGACGCACACGCGGGCTTCGAAGCGGCTGTCCTGCGCCAGTTGCAGGGCTAGCGGGGCCATCTTGATGGCTTCTGGGc                                                                                                                                   <  7:97947/148‑1 (MQ=255)
                ttCAAGTCGTATTCGGGGCTGATGCCGAACAGCTCCAGTACCTGATCGAGCATTTGCCGGTGCTGGCCGGTGACGCACACGCGGGCTTCGAAGCGGCTGTCCTGCGCCAGTTGCAGGGCTAGCGGGGCCATCTTGATGGCTTCTGGGc                                                                                                                                   <  2:144087/148‑1 (MQ=255)
                      tCGTATTCGGGGCTGATGCCGAACAGCTCCAGTACCTGATCGAGCATTTGCCGGTGCTGGCCGGTGACGCACACGCGGGCTTCGAAGCGGCTGTCCTGCGCCAGTTGCAGGGCTAGCGGGGCCATCTTGATGGCTTCTGGGCGGGTAcc                                                                                                                            <  1:302147/149‑1 (MQ=255)
                      tCGTATTCGGGGCTGATGCCGAACAGCTCCAGTACCTGATCGAGCATTTGCCGGTGCTGGCCGGTGACGCACACGCGGGCTTCGAAGCGGCTGTCCTGCGCCAGTTGCAGGGCTAGCGGGGCCATCTTGATGGCTTCTGGGCGGGTAcc                                                                                                                            >  7:294802/1‑149 (MQ=255)
                         tATTCGGGGCTGATGCCGAACAGCTCCAGTACCTGATCGAGCATTTGCCGGTGCTGGCCGGTGACGCACACGCGGGCTTCGAAGCGGCTGTCCTGCGCCAGTTGCAGGGCTAGCGGGGCCATCAAGATGGccc                                                                                                                                         <  5:213913/133‑3 (MQ=255)
                         tATTCGGGGCTGATGCCGAACAGCTCCAGTACCTGATCGAGCATTTGCCGGTGCTGGCCGGTGACGCACACGCGGGCTTCGAAGCGGCTGTCCTGCGCCAGTTGCAGGGCTAGCGGGGCCATCAAGATGGccc                                                                                                                                         >  6:213913/1‑131 (MQ=255)
                           ttCGGGGCTGATGCCGAACAGCTCCAGTACCTGATCGAGCATTTGCCGGTGCTGGCCGGTGACGCACACGCGGGCTTCGAAGCGGCTGTCCTGCGCCAGTTGCAGGGCTAGCGGGGCCATCTTGATGGCTTCTGGGCGGGTACCGaaaa                                                                                                                       <  2:221314/149‑1 (MQ=255)
                           ttCGGGGCTGATGCCGAACAGCTCCAGTACCTGATCGAGCATTTGCCGGTGCTGGCCGGTGACGCACACGCGGGCTTCGAAGCGGCTGTCCTGCGCCAGTTGCAGGGCTAGCGGGGCCATCTTGATGGCTTCTGGGCGGGTACCGaaaa                                                                                                                       <  8:294802/149‑1 (MQ=255)
                           ttCGGGGCTGATGCCGAACAGCTCCAGTACCTGATCGAGCATTTGCCGGTGCTGGCCGGTGACGCACACGCGGGCTTCGAAGCGGCTGTCCTGCGCCAGTTGCAGGGCTAGCGGGGCCATCTTGATGGCTTCTGGGCGGATACCGaaaa                                                                                                                       >  4:208553/1‑149 (MQ=255)
                              ggggCTGATGCCGAACAGCTCCAGTACCTGATCGAGCATTTGCCGGTGCTGGCCGGTGACGCACACGCGGGCTTCGAAGCGGCTGTCCTGCGCCAGTTGCAGGGCTAGCGGGGCCATCTTGATGGCTTCTGGGCGGGTACCGaaa                                                                                                                        >  6:198459/1‑145 (MQ=255)
                              ggggCTGATGCCGAACAGCTCCAGTACCTGATCGAGCATTTGCCGGTGCTGGCCGGTGACGCACACGCGGGCTTCGAAGCGGCTGTCCTGCGCCAGTTGCAGGGCTAGCGGGGCCATCTTGATGGCTTCTGGGCGGGTACCGaaa                                                                                                                        <  5:198459/145‑1 (MQ=255)
                                   tGATGCCGAACAGCTCCAGTACCTGATCGAGCATTTGCCGGTGCTGGCCGGTGACGCACACGCGGGCTTCGAAGCGGCTGTCCTGCGCCAGTTGCAGGGCTAGCGGGGCCATCTTGATGGCTTCTGGGCGGGTACCGAAAACAGTCAgg                                                                                                               >  4:293389/1‑149 (MQ=255)
                                     aTGCCGAACAGCTCCAGTACCTGATCGAGCATTTGCCGGTGCTGGCCGGTGACGCACACGCGGGCTTCGAAGCGGCTGTCCTGCGCCAGTTGCAGGGCTAGCGGGGCCATCTTGATGGCTTCTGGGCGGGTACCGAAAACAGTCAGGGt                                                                                                             >  6:22245/1‑149 (MQ=255)
                                       gCCGAACAGCTCCAGTACCTGATCGAGCATTTGCCGGTGCTGGCCGGTGACGCACACGCGGGCTTCGAAGCGGCTGTCCTGCGCCAGTTGCAGGGCTAGCGGGGCCATCTTGATGGCTTCTGGGCGGGTACCGAAAACAGTCAGGGTCt                                                                                                           >  4:107790/1‑149 (MQ=255)
                                        ccGAACAGCTCCAGTACCTGATCGAGCATTTGCCGGTGCTGGCCGGTGACGCACACGCGGGCTTCGAAGCGGCTGTCCTGCGCCAGTTGCAGGGCTAGCGGGGCCATCTTGATGGCTTCTGGGCGGGTACCGAAAACAGTCAGGGTCtt                                                                                                          <  3:242016/149‑1 (MQ=255)
                                           aaCAGCTCCAGTACCTGATCGAGCATTTGCCGGTGCTGGCCGGTGACGCACACGCGGGCTTCGAAGCGGCTGTCCTGCGCCAGTTGCAGGGCTAGCGGGGCCATCTTGATGGCTTCTGGGCGGGTACCGAAAACAGTCAGGGTCTTGAg                                                                                                       >  1:68202/1‑149 (MQ=255)
                                            aCAGCTCCAGTACCTGATCGAGCATTTGCCGGTGCTGGCCGGTGACGCACACGCGGGCTTCGAAGCGGCTGTCCTGCGCCAGTTGCAGGGCTAGCGGGGCCATCTTGATGGCTTCTGGGCGGGTACCGAAAACAGTCAGGGTCTTGAgg                                                                                                      <  2:68202/149‑1 (MQ=255)
                                                       aCCTGATCGAGCATTTGCCGGTGCTGGCCGGTGACGCACACGCGGGCTTCGAAGCGGCTGTCCTGAGCCAGTTTCAGGGCTAGCGGGGCCATCTTGATGGCTTCTGGGCGGGTACCGAAAACAGTCAGGGTCTTGAGGGGCACGGCAg                                                                                            <  2:159125/148‑1 (MQ=255)
                                                          tGATCGAGCATTTGCCGGTGCTGGCCGGTGACGCACACGCGGGCTTCGAAGCGGCTGTCCTGCGCCAGTTGCAGGGCTAGCGGGGCCATCTTGATGGCTTCTGGGCGGGTACCGAAAACAGTCAGGGTCTTGAGGGGCACGGCAGTGAt                                                                                        >  5:297796/1‑149 (MQ=255)
                                                            aTCGAGCATTTGCCGGTGCTGGCCGGTGACGCACACGCGGGCTTCGAAGCGGCTGTCCTGCGCCAGTTGCAGGGCTAGCGGGGCCATCTTGATGGCTTCTGGGTGGGTACCGAAAACAGTCAGGGTCTTGAGGGGCACGGCAGTGATc                                                                                       <  7:97020/148‑1 (MQ=255)
                                                            aTCGAGCATTTGCCGGTGCTGGCCGGTGACGCACACGCGGGCTTCGAAGCGGCTGTCCTGCGCCAGTTGCAGGGCTAGCGGGGCCATCTTGATGGCTTCTGGGCGGGTACCGAAAACAGTCAGGGTCTTGAGGGGCACGGCAGTGATc                                                                                       <  1:291997/148‑1 (MQ=255)
                                                             tCGAGCATTTGCCGGTGCTGGCCGGTGACGCACACGCGGGCTTCGAAGCGGCTGTCCTGCGCCAGTTGCAGGGCTAGCGGGGCCATCTTGATGGCTTCTGGGCGGGTACCGAAAACAGTCAGGGTCTTGAGGGGCACGGCAGTGATCct                                                                                     <  3:107790/149‑1 (MQ=255)
                                                              cGAGCATTTGCCGGTGCTGGCCGGTGACGCACACGCGGGCTTCGAAGCGGCTGTCCTGCGCCAGTTGCAGGGCTAGCGGGGCCATCTTGATGGCTTCTGGGAGGGTACCGAAAACAGTCAGGGTCTTGAGGGGCACGGCAGTGATCctc                                                                                    >  3:259309/1‑149 (MQ=255)
                                                               gAGCATTTGCCGGTGCTGGCCGGTGACGCACACGCGGGCTTCGAAGCGGCTGTCCTGCGCCAGTTGCAGGGCTAGCGGGGCCATCTTGATGGCTTCTGGGCgg                                                                                                                                 >  8:25785/1‑103 (MQ=255)
                                                               gAGCATTTGCCGGTGCTGGCCGGTGACGCACACGCGGGCTTCGAAGCGGCTGTCCTGCGCCAGTTGCAGGGCTAGCGGGGCCATCTTGATGGCTTCTGGGCgg                                                                                                                                 <  7:25785/103‑1 (MQ=255)
                                                               gAGCATTTGCCGGTGCTGGCCGGTGACGCACACGCGGGCTTCGAAGCGGCTGTCCTGCGCCAGTTGCAGGGCTAGCGGGGCCATCTTGATGGCTTCTGGGCGGGTACCGAAAACAGTCAGGGTc                                                                                                            <  5:63554/124‑1 (MQ=255)
                                                               gAGCATTTGCCGGTGCTGGCCGGTGACGCACACGCGGGCTTCGAAGCGGCTGTCCTGCGCCAGTTGCAGGGCTAGCGGGGCCATCTTGATGGCTTCTGGGCGGGTACCGAAAACAGTCAGGGTc                                                                                                            >  6:63554/1‑124 (MQ=255)
                                                                    tttGCCGGTGCTGGCCGGTGACGCACACGCGGGCTTCGAAGCGGCTGTCCTGCGCCAGTTGCAGGGCTAGCGGGGCCATCTTGATGGCTTCTGGGCGGGTACCGAAAACAGTCAGCGTCTTGAGGGGCACGGCAGTGAGCCTCTTGCTc                                                                              >  3:264750/1‑149 (MQ=255)
                                                                    tttGCCGGTGCTGGCCGGTGACGCACACGCGGGCTTCGAAGCGGCTGTCCTGCGCCAGTTGCAGGGCTAGCGGGGCCATCTTGATGGCTTCTGGGCGGATACCGAAAACAGTCAGGGTCTTGAGGATCACTGCCGTGCCCctcaagacc                                                                              <  3:208553/149‑7 (MQ=255)
                                                                             gCTGGCCGGTGACGCACACGCGGGCTTCGAAGCGGCTGTCCTGCGCCAGTTGCAGGGCTAGCGGGGCCATCTTGATGGCTTCTGGGCGGGTACCGAAAACAGTCAGGGTCTTGAGGGGCACGGCAGTGATCCTCTTGCTCGGGTCCTTg                                                                     <  5:22245/149‑1 (MQ=255)
                                                                              cTGGCCGGTGACGCACACGCGGGCTTCGAAGCGGCTGTCCTGCGCCAGTTGCAGGGCTAGCGGGGCCATCTTGATGGCTTCTGGGCGGGTACCGAAAACAGTCAGGGTCTTGAGGGGCACGGCAGTGATCCTCTTGCTCGGGTCCTTGa                                                                    <  5:20443/149‑1 (MQ=255)
                                                                                 gCCGGTGACGCACACGCGGGCTTCGAAGCGGCTGTCCTGCGCCAGTTGCAGGGCTAGCGGGGCCATCTTGATGGCTTCTGGGCGGGTACCGAAAACAGTCAGGGTCTTGAGGGGCACGGCAGTGATCCTGTTGCTCGGGTCCTTGAAg                                                                  >  7:182652/1‑148 (MQ=255)
                                                                                        aCGCACACGCGGGCTTCGAAGCGGCTGTCCTGCGCCAGTTGCAGGGCTAGCGGGGCCATCTTGATGGCTTCTGGGCGGGTACCGAAAACAGTCAGGGTCTTGAGGGGCACGGCAGTGATCCTCTTGCTCGGGTCCTTGAAGCAGGCCg                                                           <  6:297796/148‑1 (MQ=255)
                                                                                             cacGCGGGCTTCGAAGCGGCTGTCCTGCGCCAGTTGCAGGGCTAGCGGGGCCATCTTGATGGCTTCTGGGCGGGTACCGAAAACAGTCAGGGTCTTGAGGGGCACGGCAGTGATCCTCTTGCTCGGGTCCTTGAAGCAGGCCGGTTTcc                                                     <  4:18125/149‑1 (MQ=255)
                                                                                                    gCTTCGAAGCGGCTGTCCTGCGCCAGTTGCAGGGCTAGCGGGGCCATCTTGATGGCTTCTGGGCGGGTACCGAAAACAGTCAGGGTCTTGAGGGGCACGGCAGTGATCCTGTTGCTCGGGTCCTTGAAGCAGGCCGGTTTCCATTGCTc                                              <  8:182652/149‑1 (MQ=255)
                                                                                                     cTTCGAAGCGGCTGTCCTGCGCCAGTTGCAGGGCTAGCGGGGCCATCTTGATGGCTTCTGGGCGGGTACCGAAAACAGTCAGGGTCTTGAGGGGCACGGCAGTGATCCTCTTGCTCgg                                                                            <  5:131461/118‑1 (MQ=255)
                                                                                                     cTTCGAAGCGGCTGTCCTGCGCCAGTTGCAGGGCTAGCGGGGCCATCTTGATGGCTTCTGGGCGGGTACCGAAAACAGTCAGGGTCTTGAGGGGCACGGCAGTGATCCTCTTGCTCgg                                                                            >  6:131461/1‑118 (MQ=255)
                                                                                                             cGGCTTTCCTGCGCCAGTTGCAGTTCTAGCGGGGCCATCTTGATGGCTTCTGGGCGGGGACAGAAAACAGTCAGGGTCTTGAGGGGCACGGCAGTGATCCTCTTGCTCGGGTCCTTGAAGCAGGCCTGttt                                                       <  4:24865/131‑1 (MQ=255)
                                                                                                             cGGCTGTCCTGCGCCAGTTGCAGGGCTAGCGGGGCCATCTTGATGGCTTCTGGGCGGGTACCGAAAACAGTCAGGGTCTTGAGGGGCACGGCAGTGATCCTCTTGCTCGGGTCCTTGAAGCAGGCCGGTTTCCATTGCTCAAAAATCTa                                     <  3:293389/149‑1 (MQ=255)
                                                                                                               gCTGTCCTGCGCCAGTTGCAGGGCTAGCGGGGCCATCTTGATGGCTTCTGGGCGGGTACCGAAAACAGTCAGGGTCTTGAGGGGCACGGCAGTGATCCTCTTGCTCGGGTCCTTGAAGCAGGCCGGTTTCCATTGCTCAAAAATCTAg                                    >  1:276870/1‑148 (MQ=255)
                                                                                                                      tGCGCCAGTTGCAGGGCTAGCGGGGCCATCTTGATGGCTTCTGGGCGGGTACCGAAAACAGTCAGGGTCTTGAGGGGCACGGCAGTGATCCTCTTGCTCGGGTCCTTGAAGCAGGCCGGTTTCCATTGCTCAAAAATCTAGCAGGCggg                            >  8:225614/1‑149 (MQ=255)
                                                                                                                                gCAGGGCTAGCGGGGCCATCTTGATGGCTTCTGGGCGGGTACCGAAAACAGTCAGGGTCTTGAGGGGCACGGCAGTGATCCTCTTGCTCGGGTCCTTGAAGCAGGCCGGTTTCCATTGCTCAAAAATCTAGCAGGCGGGCAATGGCGt                   <  7:63128/148‑1 (MQ=255)
                                                                                                                                gCAGGGCTAGCGGGGCCATCTTGATGGCTTCTGGGCGGGTACCGAAAACAGTCAGGGTCTTGAGGGGCACGGCAGTGATCCTCTTGCTCGGGTCCTTGAAGCAGGCCGGTTTCCATTGCTCAAAAATCTAGCAGGCGGGCAATGGCGTg                  >  6:2526/1‑149 (MQ=255)
                                                                                                                                        ccagaagCCATCAAGATGGCTTCTGGGCGGGTACCGAAAACAGTCAGGGTCTTGAGGGGCACGGCAGTGATCCTCTTGCTCGGGTCCTTGAAGCAGGCCGGTTTCCATTGCTCAAAAATCt                                      >  2:341056/7‑121 (MQ=255)
                                                                                                                                        ccagaagCCATCAAGATGGCTTCTGGGCGGGTACCGAAAACAGTCAGGGTCTTGAGGGGCACGGCAGTGATCCTCTTGCTCGGGTCCTTGAAGCAGGCCGGTTTCCATTGCTCAAAAATCt                                      <  1:341056/115‑1 (MQ=255)
                                                                                                                                         gCGGGGCCATCTTGATGGCTTCTGGGCGGGGACCGAAAACAGTCAGTGTCTTGAGGGGCACGGCAGTGATCCTCTTGCTCGGGTCCTTGAAGCAGGCCTGTTTCCATTGCTCAAAAATCTAGCAGGCGGGCAATGGCGTGCCAGttt           <  4:259309/147‑1 (MQ=255)
                                                                                                                                            gggCCATCTTGATGGCTTCTGGGCGGGTACCGAAAACAGTCAGGGTCTTGAGGGGCACGGCAGTGATCCTCTTGCTCGGGTCCTTGAAGCAGGCCGGTTTCCATTGCTCAAAAATCTAGCAGGCGGGCAATGGCGTGCCAGTTTGTCgg      >  4:161729/1‑149 (MQ=255)
                                                                                                                                            gggCCATCTTGATGGCTTCTGGGCGGGTACCGAAAACAGGCAGGGTCTGGAGGGGCACGGCAGTGATCCTCTTGCTCGGGTCCTTGAAGCAGGCCGGTTTCCATTGCTCAAAAATCTAGCAGGCGGGCAATGGCGTGCCAGTTTGTCgg      <  6:174451/149‑1 (MQ=255)
                                                                                                                                              gCCATCTTGATGGCTTCTGGGCGGGTACCGAAAACAGTCAGGGTCTTGAGGGGCACGGCAGTGATCCTCTTGCTCGGGTCCTTGAAGCAGGCCGGTTTCCATTGCTCAAAAATATAGCAGGCGGGCAATTGCGTGCCAGTTTGTCgggg    >  5:58882/1‑149 (MQ=255)
                                                                                                                                                cATCTTGATGGCTTCTGGGCGGGTACCGAAAACAGTCAGGGTCTTGAGGGGCACGGCAGTGATCCTCTTGCTCGGGTCCTTGAAGCAGGCCGGTTTCCATTGCTCAAAAATCTAGCAGGCGGGCAATGGCGTGCCAGTTTGTCgggggg  <  5:338204/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
GCCGGCTTTCATGATGTTCAAGTCGTATTCGGGGCTGATGCCGAACAGCTCCAGTACCTGATCGAGCATTTGCCGGTGCTGGCCGGTGACGCACACGCGGGCTTCGAAGCGGCTGTCCTGCGCCAGTTGCAGGGCTAGCGGGGCCATCTTGATGGCTTCTGGGCGGGTACCGAAAACAGTCAGGGTCTTGAGGGGCACGGCAGTGATCCTCTTGCTCGGGTCCTTGAAGCAGGCCGGTTTCCATTGCTCAAAAATCTAGCAGGCGGGCAATGGCGTGCCAGTTTGTCGGGGGG  >  NC_002947/2039282‑2039574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑