Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,060,625 G→A 7.8% P2S (CCA→TCA)  PP_1836 ← ZIP family metal transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,060,6250GA 7.8% 142.8 / 2.6 52P2S (CCA→TCA) PP_1836ZIP family metal transporter
Reads supporting (aligned to +/- strand):  ref base G (21/26);  new base A (2/2);  total (23/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.16e-01

CCGCGTTCCAGAGGCTGGCGGCCAGCAGTACGCACACCGCCAGCAGGCTCAGCCCCAGGCCGGCACTGAGCCA‑GGGGGTATCGATGGCCTGTTGGCGCCAGGCCTGCAGCAGTGAAGGTGGGGCGGCGCTTTGGCTATGGGTGGGTGGCATGCGAACCTCGAATCAGTGAATGCAGTCAGTCTACCGAGGGACCTGCGACATCCGCCAAGGATTCCCTTCTATTGGCGCGATAGCTGACTATGCTGTGCAGCAAAGCCTCTGGACAGGAAATCAGACATGGGATCTAC  >  NC_002947/2060478‑2060765
                                                                                                                                                    |                                                                                                                                            
ccGCGTTCCAGAGGCTGGCGGCCAGCAGTACGCACACCGCCAGCAGGCTCAGCCCCAGGCCGGCACTGAGCCA‑GGGGGTATCGATGGCCTGTTGGCGCCAGGCCTGCAGCAGTGAAGGTGGGGCGGCGCTTTGGCTATGGgtgggtgg                                                                                                                                              >  7:65132/1‑148 (MQ=255)
ccGCGTTCCAGAGGCTGGCGGCCAGCAGTACGCACACCGCCAGCAGGCTCAGCCCCAGGCCGGCACTGAGCCA‑GGGGGTATCGATGGCCTGTTGGCGCCAGGCCTGCAGCAGTGAAGGTGGGGCGGCGCTTTGGCTATGGgtgggtgg                                                                                                                                              >  2:165136/1‑148 (MQ=255)
ccGCGTTCCAGAGGCTGGCGGCCAGCAGTACGCACACCGCCAGCAGGCTCAGCCCCAGGCCGGCACTGAGCCA‑GGGGGTATCGATGGCCTGTTGGCGCCAGGCCTGCAGCAGTGAAGGTGGGGCGGCGCTTTGGCTATGGGTGGGTGGc                                                                                                                                             <  3:134616/149‑1 (MQ=255)
 cgcgTTCCAGAGGCTGGCGGCCAGCAGTACGCACACCGCCAGCAGGCTCAGCCCCAGGCCGGCACTGAGCCA‑GGGGGTATCGATGGCCTGTTGGCGCCAGGCCTGCAGCAGTGAAGGTGGGGCGGCGCTTTGGCTATGGGTGGGTGGCa                                                                                                                                            >  3:20748/1‑149 (MQ=255)
    gTTCCAGAGGCTGGCGGCCAGCAGTACGCACACCGCCAGCAGGCTCAGCCCCAGGCCGGCACTGAGCCA‑GGGGGTATCGATGGCCTGTTGGCGCCAGGCCTGCAGCAGTGAAGGTGGGGCGGCGCTTTGGCTATGGGTGGGTGGCATGc                                                                                                                                         <  8:65132/149‑1 (MQ=255)
          gagGCTGGCGGCCAGCAGTACGCACACCGCCAGCAGGCTCAGCCCCAGGCCGGCACTGAGCCA‑GGGGGTATCGATGGCCTGTTGGCGCCAGGCCTGCAGCAGTGAAGGTGGGGCGGCGCTTTGGCTATGGGTGGGTGGCATGCGAACCt                                                                                                                                   <  2:223590/149‑1 (MQ=255)
          gagGCTGGCGGCCAGCAGTACGCACACCGCCAGCAGGCTCAGCCCCAGGCCGGCACTGAGCCA‑GGGGGTATCGATGGCCTGTTGGCGCCAGGCCTGCAGCAGTGAAGGTGGGGCGGCGCTTTGGCTATGGGTGGGTGGCATGCGAACCt                                                                                                                                   >  2:21039/1‑149 (MQ=255)
            ggCTGGCGGCCAGCAGTACGCACACCGCCAGCAGGCTCAGCCCCAGGCCGGCACTGAGCCA‑GGGGGTATCGATGGCCTGTTGGCGCCAGGCCTGCAGCAGTGAAGGTGGGGCGGCGCTTTGGCTATGGGTGGGTGGCATGCGAACCTCg                                                                                                                                 <  1:239099/149‑1 (MQ=255)
               tGGCGGCCAGCAGTACGCACACCGCCAGCAGGCTCAGCCCCAGGCCGGCACTGAGCCA‑GGGGGTATCGATGGCCTGTTGGCGCCAGGCCTGCAGCAGTGAAGGTGGGGCGGCGCTTTGGCTATGGGTGGGTGGCATGCGAACCTCGAAt                                                                                                                              <  4:20748/149‑1 (MQ=255)
               tGGCGGCCAGCAGTACGCACACCGCCAGCAGGCTCAGCCCCAGGCCGGCACTGAGCCA‑GGGGGTATCGATGGCCTGTTGGCGCCAGGCCTGCAGCAGTGAAGGTGGGGCGGCGCTTTGGCTATGGGTGGGTGGCATGCGAACCTCGAAt                                                                                                                              >  2:171959/1‑149 (MQ=255)
                 gcggcCAGCAGTACGCACACCGCCAGCAGGCTCAGCCCCAGGCCGGCACTGAGCCA‑GGGGGTATCGATGGCCTGTTGGCGCCAGGCCTGCAGCAGTGAAGGTGGGGCGGCGCTTTGGCTATGGGTGGGTGGCATGCGAACCTCGAAt                                                                                                                              <  1:171959/147‑1 (MQ=255)
                  cggcCAGCAGTACGCACACCGCCAGCAGGCTCAGCCCCAGGCCGGCACTGAGCCA‑GGGGGTATCGATGGCCTGTTGGCGCCAGGCCTGCAGCAGTGAAGGTGGGGCGGCGCTTTGGCTATGGGTGGGTGGCATGCGAACCTCGAATCAg                                                                                                                           <  1:165136/149‑1 (MQ=255)
                  cggcCAGCAGTACGCACACCGCCAGCAGGCTCAGCCCCAGGCCGGCACTGAGCCA‑GGGGGTATCGATGGCCTGTTGGCGCCAGGCCTGCAGCAGTGAAGGTGGGGCGGCGCTTTGGCTATGGGTGGGTGGCATGCGAACCTCGAATCAg                                                                                                                           >  2:200083/1‑149 (MQ=255)
                   ggcCAGCAGTACGCACACCGCCAGCAGGCTCAGCCCCAGGCCGGCACTGAGCCA‑GGGGGTATCGATGGCCTGTTGGCGCCAGGCCTGCAGCAGTGAAGGTGGGGCGGCGCTTTGGCTATGGGTGGGTGGCATGCGAACCTc                                                                                                                                  >  5:92600/1‑141 (MQ=255)
                   ggcCAGCAGTACGCACACCGCCAGCAGGCTCAGCCCCAGGCCGGCACTGAGCCA‑GGGGGTATCGATGGCCTGTTGGCGCCAGGCCTGCAGCAGTGAAGGTGGGGCGGCGCTTTGGCTATGGGTGGGTGGCATGCGAACCTc                                                                                                                                  <  6:92600/141‑1 (MQ=255)
                   ggcCAGCAGTACGCACACCGCCAGCAGGCTCAGCCCCAGGCCGGCACTGAGCCA‑GGGGGTATCGATGGCCTGTTGGCGCCAGGCCTGCAGCAGTGAAGGTGGGGCGGCGCTTTGGCTATGGGTGGGTGGCATGCGAACCTCGAATCAGt                                                                                                                          <  2:140103/149‑1 (MQ=255)
                   ggcCAGCAGTACGCACACCGCCAGCAGGCTCAGCCCCAGGCCGGCACTGAGCCA‑GGGGGTATCGATGGCCTGTTGGCGCCAGGCCTGCAGCAGTGAAGGTGGGGCGGCGCTTTGGCTATGGGTGGGTGGCATGCGAACCTCGAATCAGt                                                                                                                          <  1:21039/149‑1 (MQ=255)
                     ccAGCAGTACGCACACCGCCAGCAGGCTCAGCCCCAGGCCGGCACTGAGCCA‑GGGGGTATCGATGGCCTGTTGGCGCCAGGCCTGCAGCAGTGAAGGTGGGGCGGCGCTTTGGCTATGGGTGGGTGGCATGCGAACCTCGAATCAGTg                                                                                                                         >  1:335053/1‑148 (MQ=255)
                     ccAGCAGTACGCACACCGCCAGCAGGCTCAGCCCCAGGCCGGCACTGAGCCA‑GGGGGTATCGATGGCCTGTTGGCGCCAGGCCTGCAGCAGTGAAGGTGGGGCGGCGCTTTGGCTATGGGTGGGTGGCATGCGAACCTCGAATCAGTg                                                                                                                         >  1:126501/1‑148 (MQ=255)
                            tACGCACACCGCCAGCAGGCTCAGCCCCAGGCCGGCACTGAGCCAGGGGGGTATCGATGGCATGTTGGCGCCAGGCCTGAAGAAGTGAAGGTGGGGCGGCGATTTGGATATGGGTGGGTGGCATGCGAACATCGAATAATTGAATGcag                                                                                                                  >  1:254664/1‑149 (MQ=255)
                             aCGCACACCGCCAGCAGGCTCAGCCCCAGGCCGGCACTGAGCCA‑GGGGGTATCGATGGCCTGTTGGCGCCAGGCCTGCAGCAGTGAAGGTGGGGCGGCGCTTTGGCTATGGGTGGGTGGCATGCGAACCTCGAATCAGTGAATGcagt                                                                                                                 <  5:125647/148‑1 (MQ=255)
                              cGCACACCGCCAGCAGGCTCAGCCCCAGGCCGGCACTGAGCCA‑GGGGGTATCGATGGCCTGTTGGCGCCAGGCCTGCAGCAGTGAAGGTGGGGCGGCGCTTTGGCTATGGGTGGGTGGCATGCGAACCTCGAATCAGTGAATGcagtca                                                                                                               <  6:273561/149‑1 (MQ=255)
                                                    ccccAGGCCGGCACTGAGCCA‑GGGGGTATCGATGGCCTGTTGGCGCCAGGCCTGCAGCAGTGAAGGTGGGGCGGCGCTTTGGCTATGGGTGGGTGGCATGCGAACCTCGAATCAGTGAATGCAGTCAGTCTACCGAGGGACCTGCGACa                                                                                         >  8:262657/1‑149 (MQ=255)
                                                    ccccAGGCCGGCACTGAGCCA‑GGGGGTATCGATGGCCTGTTGGCGCCAGGCCTGCAGCAGTGAAGGTGGGGCGGCGCTTTGGCTATGGGTGGGTGGCATGCGAACCTCGAATCAGTGAATGCAGTCAGTCTACCGAGGGACCTGCGACa                                                                                         >  1:224637/1‑149 (MQ=255)
                                                      ccAGGCCGGCACTGAGCCA‑GGGGGTATCGATGGCCTGTTGGCGCCAGGCCTGCAGCAGTGAAGGTGGGGCGGCGCTTTGGCTATGGGTGGGTGGCATGCGAACCTCGAATCAGTGAATGCAGTCAGTCTACCGAGGGACCTGCGACATc                                                                                       <  2:126501/149‑1 (MQ=255)
                                                      ccAGGCCGGCACTGAGCCA‑GGGGGTATCGATGGCCTGTTGGCGCCAGGCCTGCAGCAGTGAAGGTGGGGCGGCGCTTTGGCTATGGGTGGGTGGCATGCGAACCTCGAATCAGTGAATGCAGTCAGTCTACCGAGGGACCTGCGACATc                                                                                       <  8:109514/149‑1 (MQ=255)
                                                       cAGGCCGGCACTGAGCCA‑GGGGGTATCGATGGCCTGTTGGCGCCAGGCCTGCAGCAGTGAAGGTGGGGCGGCGCTTTGGCTATGGGTGGGTGGCATGCGAACCTCGAATCAGTGAATGCAGTCAGTCTACCGAGGGACCTGCGACATcc                                                                                      <  2:335053/149‑1 (MQ=255)
                                                            cGGCACTGAGCCA‑GGGGGTATCGATGGCCTGTTGGCGCCAGGCCTGCAGCAGTGAAGGTGGGGCGGCGCTTTGGCTATGGGTGGGTGGCATGCGAACCTCGAATCAGTGAATGCAGTCAGTCTACCGAGGGACCTGCGACATCCGcc                                                                                   >  2:102412/1‑147 (MQ=255)
                                                            cGGCACTGAGCCA‑GGGGGTATCGATGGCCTGTTGGCGCCAGGCCTGCAGCAGTGAAGGTGGGGCGGCGCTTTGGCTATGGGTGGGTGACATGCGAACCTCGAATCAGTGAATGCAGTCAGTCTACCGAGGGACCTGCGACATCCGcc                                                                                   >  6:188691/1‑147 (MQ=255)
                                                            cGGCACTGAGCCA‑GGGGGTATCGATGGCCTGTTGGCGCCAGGCCTGCAGCAGTGAAGGTGGGGCGGCGCTTTGGCTATGGGTGGGTGACATGCGAAACTCGAATCAGTGAATGCAGTCAGTCTACCGAGGGACCTGCGACATACgac                                                                                   >  6:183843/1‑145 (MQ=255)
                                                             ggCACTGAGCCA‑GGGGGTATCGATGGCCTGGTGGCGCCAGGCCTGCAGCAGTGAAGGTGGGGCGGCGCTTTGGCTATGGGTGGGTGGCATGCGAACCTCGAATCAGTGAATGCAGTCAGTCTACCGAGGGACCTGCGACATCCGCCAAg                                                                                <  2:254664/149‑1 (MQ=255)
                                                                     gCCT‑GGGTGTATCGATGGCCTGTTGGGGCCAGGCCTGCAGCAGTGAAGGTGGGGCGGCGCTTTGGCTATGGGTGGGTGGCATGCGAACCTCGAATCAGTGAATGCAGTCAGTCTACCGAGGGACCTGCGACATCCGCCAAGGATTccc                                                                         <  5:261083/148‑1 (MQ=255)
                                                                     gCCA‑GGGGGTATCGATGGCCTGTTGGCGCCAGGCCTGCAGCAGTGAAGGTGGGGCGGCGCTTTGGCTATGGGTGGGTGGCATGCGAACCTCGAATCAGTGAATGCAGTCAGTCTACCGAGGGACCTGCGACATCCGCCAAGGATTccgt                                                                        >  7:294840/1‑147 (MQ=255)
                                                                           ggtgTATCGATGGGCTGTTGGCGCCTGGGGTGCATCAGTTAAGGTGGGGAGGCGCTTAGGCTATTGGTGGGTGTCATGCGAACCTCGAATCAGTGATTGCAGTCAGTCTACCGAGGGACCTGCTACTTCCGCCAAGGATTCCCTTCTAt                                                                   <  4:260687/146‑1 (MQ=255)
                                                                                 tCGATGGCCTGTTGGCGCCAGGCCTGCAGCAGTGAAGGTGGGGCGGCGCTTTGGCTATGGGTGGGTGGCATGCGAACCTCGAATCAGTGAATGCAGTCAGTCTACCGAGGGACCTGCGACATCCGCCAAGGATTCCCTTCTATTGgcgc                                                             <  3:161139/149‑1 (MQ=255)
                                                                                      ggCCTGTTGGCGCCAGGCCTGCAGCAGTGAAGGTGGGGCGGCGCTTTGGCTATGGGTGGGTGGCATGCGAACCTCGAATCAGTGAATGCAGTCAGTCTACCGAGGGACCTGCGACATCCGCCAAGGATTCCCTTCTAtt                                                                  >  3:21860/1‑139 (MQ=255)
                                                                                      ggCCTGTTGGCGCCAGGCCTGCAGCAGTGAAGGTGGGGCGGCGCTTTGGCTATGGGTGGGTGGCATGCGAACCTCGAATCAGTGAATGCAGTCAGTCTACCGAGGGACCTGCGACATCCGCCAAGGATTCCCTTCTAtt                                                                  <  4:21860/139‑1 (MQ=255)
                                                                                         cTGTTGGCGCCAGGCCTGCAGCAGTGAAGGTGGGGCGGCGCTTTGGCTATGGGTGGGTGGCATGCGAACCTCGAATCAGTGAATGCAGTCAGTCTACCGAGGGACCTGCGACATCCGCCAAGGATTCCCTTCTATTGGCGCGATAGCt                                                      >  5:95961/1‑148 (MQ=255)
                                                                                            gcgccaacAGGCCTGCAGCAGTGAAGGTGGGGCGGCGCTTTGGCTATGGGTGGGTGGCATGCGAACCTCGAATCAGTGAATGCAGTCAGTCTACCGAGGGACCTGCGACATCCGCCAAGGATTCCCTTCTATTGGCGCGATAGCTGa                                                    <  7:277935/140‑1 (MQ=255)
                                                                                              ggCGCCAGGCCTGCAGCAGTGAAGGTGGGGCGGCGCTTTGGCTATGGGTGGGTGGCATGCGAACCTCGAATCAGTGAATGCAGTCAGTCTACCGAGGGACCTGCGACATCCGCCAAGGATTCCCTTCTATTGGCGCGATAGCTGACTAt                                                >  3:137269/1‑149 (MQ=255)
                                                                                               tcgcCAGGCCTGCAGCAGTGAAGGTGGGGCGGCGCTTTGGCTATGGGTGGGTGGCATGCGAACCTCGAATCAGTGAATGCAGTCAGTCTACCGAGGGACCTGCGACATCCGCCAAGGATTCCCTTCTATTGGCGCGATAGCTGACTa                                                 <  5:162315/146‑1 (MQ=255)
                                                                                                 gcCAGGCCTGCAGCAGTGAAGGTGGGGCGGCGCTTTGGCTATGGGTGGGTGGCATGCGAACCTCGAATCAGTGAATGCAGTCAGTCTACCGAGGGACCTGCGACATCCGCCAAGGATTCCCTTCTATTGGCGCGATAGCTGACTa                                                 >  6:162315/1‑145 (MQ=255)
                                                                                                    aGGCCTGCAGCAGTGAAGTGGGGGCGGCGCTTTGGCTATGTGTGGGTGACATGCGAACCTCGAATCAGTGAATGCAGTCAGGCTACCGAGGGACCTGCGACATCCGCCAAGGATTCCCTTCTATTGGCGCGATAGCTGACTATGCtgtg                                          <  5:183843/149‑1 (MQ=255)
                                                                                                    aGGCCTGCAGCAGTGAAGGTGGGGCGGCGCTTTGGCTATGGGTGGGTGACATGCGAACCTCGAATCAGTGAATGCAGTCAGTCTACCGAGGGACCTGCGACATCCGCCAAGGATTCCCTTCTATTGGCGCGATAGCTGACTATGCtgtg                                          <  5:188691/149‑1 (MQ=255)
                                                                                                     ggCCTGCAGCAGTGAAGGTGGGGCGGCGCTTTGGCTATGGGTGGGTGGCATGCGAACCTCGAATCAGTGAATGCAGTCAGTCTACCGAGGGACCTGCGACATCCGCCAAGGATTCCCTTCTATTGGCGCGATAGCTGACTATGCTGTgc                                         <  7:63367/149‑1 (MQ=255)
                                                                                                     ggCCTGCAGCAGTGAAGGTGGGGCGGCGCTTTGGCTATGGGTGGGTGGCATGCGAACCTCGAATCAGTGAATGCAGTCAGTCTACCGAGGGACCTGCGACATCCGCCAAGGATTCCCTTCTATTGGCGCGATAGCTGACTATGCTGTgc                                         <  8:66217/149‑1 (MQ=255)
                                                                                                                   aaGGTGGTGCGGCTCTTTGGATATGGGTGGGTGGCATGCGAACCTCGAATCAGTGAATGCAGTCAGTCTACCGAGGGACCTGCGACATCCGCCATGGATTCCCTTCTATTGGCGCTATAGCTGACTATGCTGTGCAGCAAAGCCTCTg                            <  3:88159/148‑1 (MQ=255)
                                                                                                                   aaGGTGGGGCGGCGCTTTGGCTATGGGTGGGTGGCATGCGAACCTCGAATCAGTGAATGCAGTCAGTCTACCGAGGGACCTGCGACATCCGCCAAGGATTCCCTTCTATTGGCGCGATAGCTGACTATGCTGTGCAGCAAAGCCTCTg                            <  6:167202/148‑1 (MQ=255)
                                                                                                                         gggCGGCGCTTTGGCTATGGGTGGGTGGCATGCGAACCTCGAATCAGTGAATGCAGTCAGTCTACCGAGGGACCTGCGACATCCGCCAAGGATTCCCTTCTATTGGCGCGATAGCTGACTATGCTGTGCAGCAAAGCCTCTGGACAgg                      >  8:40614/1‑148 (MQ=255)
                                                                                                                                  tttGTCAATGGGTGGGTGGCATGCGAACCTCGAATCAGTGAATGCAGTCAGTCTACCCAGGGACCTGCGACATCCGCCAAGGATTCCCTTCTATTGGCGCGATAGCTGACTATGCTGTGCAGCAAAGCCTCTGGACAGGAAATCAGACa            >  8:56510/1‑149 (MQ=255)
                                                                                                                                   ttGGCTATGGGTGGGTGGCATGCGAACCTCGATTCAGTGAATGCAGTCAGTGTACCGAGGGACCTGCGACATCCGCCAAGGATTCCCTTCTATTGGCGCGATAGCTGCCTATGCTGTGCAGCTAAGCCTCTGGACAGGAAAGCAGACAt           >  6:217595/1‑149 (MQ=255)
                                                                                                                                      gCTATGGGTGGGTGGCATGCGAACCTCGAATCAGTGAATGCAGTCAGTCTACCGAGGGACCTGCGACATCCGCCAAGGATTCCCTTCTATTGGCGCGATAGCTGACTATGCTGTGCAGCAAAGCCTCTGGACAGGAAATCAGACATggg        <  1:200083/149‑1 (MQ=255)
                                                                                                                                      gCTATGGGTGGGTGGCATGCGAACCTCGAATCAGTGAATGCAGTCAGTCTACCGAGGGACCTGCGACATCCGCCAAGGATTCCCTTCTATTGGAGCGATAGCTGACTATGCTGTGCAGCAAAGCCTCTGGACAGGAAATCAGACATgg         >  5:254570/1‑148 (MQ=255)
                                                                                                                                            ggtgggtgGCATGCGAACCTCGAATCAGTGAATGCAGTCAGTCTACCGAGGGACCTGCGACATCCGCCAAGGATTCCCTTCTATTGGCGCGATAGCTGACTATGCTGTGCAGCAAAGCCTCTGGACAGGAAATCAGACATGGGATCTAc  >  6:115584/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                            
CCGCGTTCCAGAGGCTGGCGGCCAGCAGTACGCACACCGCCAGCAGGCTCAGCCCCAGGCCGGCACTGAGCCA‑GGGGGTATCGATGGCCTGTTGGCGCCAGGCCTGCAGCAGTGAAGGTGGGGCGGCGCTTTGGCTATGGGTGGGTGGCATGCGAACCTCGAATCAGTGAATGCAGTCAGTCTACCGAGGGACCTGCGACATCCGCCAAGGATTCCCTTCTATTGGCGCGATAGCTGACTATGCTGTGCAGCAAAGCCTCTGGACAGGAAATCAGACATGGGATCTAC  >  NC_002947/2060478‑2060765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑