Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,063,201 G→A 11.3% intergenic (+58/‑149) PP_1838 → / → PP_1839 membrane protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,063,2010GA11.3% 89.1 / 2.8 40intergenic (+58/‑149)PP_1838/PP_1839membrane protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (13/19);  new base A (2/2);  total (18/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.34e-01

TCAAGAAGCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGC‑TTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGA  >  NC_002947/2063058‑2063336
                                                                                                                                                |                                                                                                                                       
tCAAGAAGCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGC‑TTGCCCGCTCGCACAGGACCTGCACAAGGtcc                                                                                                                                    >  4:251098/1‑147 (MQ=38)
  aagaagCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGC‑TTGCCCGCTCCCACAGGCATTGCACAGGGTTc                                                                                                                                    <  3:158669/147‑1 (MQ=255)
  aagaagCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGC‑TTGCCCGCTCCCACAGGCATTGCACAGGGTTCgg                                                                                                                                  <  5:286149/149‑1 (MQ=255)
    gaagCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGC‑TTGCCCGCTCCCACAGGCATTGCACAgg                                                                                                                                        <  6:350509/141‑1 (MQ=255)
    gaagCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGC‑TTGCCCGCTCCCACAGGCATTGCACAgg                                                                                                                                        >  5:350509/1‑141 (MQ=255)
    gaagCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGC‑TTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGAc                                                                                                                                >  3:155730/1‑149 (MQ=255)
    gaagCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGC‑TTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGAc                                                                                                                                <  4:155730/149‑1 (MQ=255)
           gAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGC‑TTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGc                                                                                                                         <  6:129115/149‑1 (MQ=255)
              gCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGACTAGCGGCCCATTAGCGGGC‑TTGCCCGCTCACACAGGCATTGCACACGGTTCGGACCTTGTGCAgg                                                                                                                      >  1:43128/1‑149 (MQ=255)
                gTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGC‑TTGCCCGCTCCCACAGGCATTGCACAGGGTTc                                                                                                                                    <  6:79991/133‑1 (MQ=255)
                gTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGC‑TTGCCCGCTCCCACAGGCATTGCACAGGGTTc                                                                                                                                    >  5:79991/1‑133 (MQ=255)
                 tataTCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGC‑TTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTcc                                                                                                                   <  3:31846/149‑1 (MQ=255)
                    atCCAGACCGCGTCCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGC‑TTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCtgt                                                                                                                >  5:298444/1‑149 (MQ=255)
                            cgcgACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGC‑TTGCCCGCTCGCACAGGACCTGCACAAGGTCCGAACCCTGTGCAATGCCTGTGGGAGCgg                                                                                                        <  3:251098/149‑1 (MQ=18)
                            cgcgACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGC‑TTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCgg                                                                                                        <  1:36059/149‑1 (MQ=255)
                               gACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGC‑TTTCCCGCTACCACAAGCAATTCACACGGTTCGGACCTTGTGCAGGTCCTCTGCGAGCCGGc                                                                                                      >  3:250450/1‑148 (MQ=255)
                                  cAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGC‑GTGCCCGCGCCCACAGGCATTGCACACGGGTCGGACCGTGTGGAGGTGCGGTGCCAGCGGGCAAGc                                                                                                  >  1:135659/1‑149 (MQ=255)
                                      agCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGC‑TTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCcgc                                                                                              <  6:298444/149‑1 (MQ=255)
                                           gctgGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGC‑TTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATg                                                                                         >  8:80293/1‑149 (MQ=255)
                                            ctgGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGC‑TGGCCCGCTCCCACCGCCATTGCACACGGTTCGGCCCTTGTCCTGGTCCGGTGCGAGCGGGCAAGCCCGCGAATg                                                                                         <  5:192616/148‑1 (MQ=255)
                                                    cacCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGC‑TTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGca                                                                                >  3:88228/1‑149 (MQ=255)
                                                     acCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGC‑TTGCCCGCGGCCCCAGGCAGTGGCCAGGGGTGGGGCCTTGTGGCGGTCCCGTGCGGGCGGGCAAGCCCGCGCAAGGGGCCCggc                                                                                >  3:90760/1‑146 (MQ=255)
                                                              gtggttCAGGGAACACCGTATTAACCCGGTGCGGCGTTGCGGCCCATTCGCGGGT‑TTGCCCGCTCCCACAGGCTTTGCACAGTGTTCGGACTTTGTGCTGTTCGTGTGGGAGCGGGAAAGCCCGCGAATGGGCCCCGCACAATTTTccc                                                                      <  4:250450/149‑1 (MQ=255)
                                                                  tgCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGC‑TTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGAc                                                                  >  2:42590/1‑149 (MQ=255)
                                                                      cGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGC‑TTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGaa                                                              <  1:42590/149‑1 (MQ=255)
                                                                             ccGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGC‑TTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATc                                                       <  7:80293/149‑1 (MQ=255)
                                                                              cGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGC‑TTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGc                                                                                                      >  1:319423/1‑101 (MQ=255)
                                                                              cGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGC‑TTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGc                                                                                                      <  2:319423/101‑1 (MQ=255)
                                                                              cGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGC‑TTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATcc                                                      <  3:291578/149‑1 (MQ=255)
                                                                                     aaCCGGGGCCGCGTTGCGGCCCATTAGCGGGCATTCCCCGAGCCCCCCGGCA‑TGCACAGGGTTAGGGCCATTTTCAGGGCCGGGGCGGCCCGGGAAGGCCGCGGATGGGGCCCGCACAATTTTACCGGCCGGGACATTTTAcccccccc                                               >  2:69044/1‑149 (MQ=255)
                                                                                            gCCGCGTTGCGGCCCATTCGCGGGC‑TTGCCCGCTCCCACAGCCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCa                                        <  3:121152/149‑1 (MQ=255)
                                                                                                    gCGGCCCATCCGCGGGC‑TTGCCCGCGCCCACCGCCCTTGCACCGGGTCCGCACCTCGTCCAGGACCTGGGCTCGCGGGCAACCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCa                                <  6:192593/149‑1 (MQ=255)
                                                                                                                   gC‑TTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTg                 <  4:88228/149‑1 (MQ=255)
                                                                                                                    c‑TTGCCCGCTCCCTCAGGCATTGCACAGGGTGCGGACCTTGTGCAGGTCCTGTGCGAGCGTGCAAGCCCGCGAATGTGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTgg                <  2:43128/149‑1 (MQ=255)
                                                                                                                            gCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTACCGGACTGAACATTATCCCCGCCCGAAGGTCAGTTATTCAGGCATAAAACACCTGGGGTTCGt         >  6:90969/1‑149 (MQ=39)
                                                                                                                                 cacaGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACt    >  4:49478/1‑149 (MQ=255)
                                                                                                                                        cctGCACAAGGTCCGAACCCTGTGCAATGCCTGTGGGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTga  <  2:322497/142‑1 (MQ=25)
                                                                                                                                        cctGCACAAGGTCCGAACCCTGTGCAATGCCTGTGGGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTga  >  1:322497/3‑144 (MQ=25)
                                                                                                                                           gCACAGGGTTCGGACTTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATca                         >  2:130919/1‑118 (MQ=255)
                                                                                                                                           gCACAGGGTTCGGACTTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATca                         <  1:130919/118‑1 (MQ=255)
                                                                                                                                             acaGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTc                                 >  6:45314/1‑108 (MQ=255)
                                                                                                                                             acaGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTc                                 <  5:45314/108‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                       
TCAAGAAGCTGGAAGCGTATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGC‑TTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGA  >  NC_002947/2063058‑2063336

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑