| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,063,221 | T→G | 11.6% | intergenic (+78/‑129) | PP_1838 → / → PP_1839 | membrane protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,063,221 | 0 | T | G | 11.6% | 75.4 / 3.2 | 34 | intergenic (+78/‑129) | PP_1838/PP_1839 | membrane protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (13/16); new base G (2/2); total (15/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.05e-01 | |||||||||||
TATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGC‑TTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCAATGAAAGAGT > NC_002947/2063075‑2063359 | tataTCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGC‑TTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTcc < 3:31846/149‑1 (MQ=255) atCCAGACCGCGTCCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGC‑TTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCtgt > 5:298444/1‑149 (MQ=255) cgcgACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGC‑TTGCCCGCTCGCACAGGACCTGCACAAGGTCCGAACCCTGTGCAATGCCTGTGGGAGCgg < 3:251098/149‑1 (MQ=18) cgcgACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGC‑TTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCgg < 1:36059/149‑1 (MQ=255) gACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGC‑TTTCCCGCTACCACAAGCAATTCACACGGTTCGGACCTTGTGCAGGTCCTCTGCGAGCCGGc > 3:250450/1‑148 (MQ=255) cAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGC‑GTGCCCGCGCCCACAGGCATTGCACACGGGTCGGACCGTGTGGAGGTGCGGTGCCAGCGGGCAAGc > 1:135659/1‑149 (MQ=255) agCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGC‑TTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCcgc < 6:298444/149‑1 (MQ=255) gctgGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGC‑TTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATg > 8:80293/1‑149 (MQ=255) ctgGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGC‑TGGCCCGCTCCCACCGCCATTGCACACGGTTCGGCCCTTGTCCTGGTCCGGTGCGAGCGGGCAAGCCCGCGAATg < 5:192616/148‑1 (MQ=255) cacCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGC‑TTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGca > 3:88228/1‑149 (MQ=255) acCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGC‑TTGCCCGCGGCCCCAGGCAGTGGCCAGGGGTGGGGCCTTGTGGCGGTCCCGTGCGGGCGGGCAAGCCCGCGCAAGGGGCCCggc > 3:90760/1‑146 (MQ=255) gtggttCAGGGAACACCGTATTAACCCGGTGCGGCGTTGCGGCCCATTCGCGGGT‑TTGCCCGCTCCCACAGGCTTTGCACAGTGTTCGGACTTTGTGCTGTTCGTGTGGGAGCGGGAAAGCCCGCGAATGGGCCCCGCACAATTTTccc < 4:250450/149‑1 (MQ=255) tgCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGC‑TTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGAc > 2:42590/1‑149 (MQ=255) cGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGC‑TTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGaa < 1:42590/149‑1 (MQ=255) ccGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGC‑TTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATc < 7:80293/149‑1 (MQ=255) cGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGC‑TTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGc < 2:319423/101‑1 (MQ=255) cGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGC‑TTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGc > 1:319423/1‑101 (MQ=255) cGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGC‑TTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATcc < 3:291578/149‑1 (MQ=255) aaCCGGGGCCGCGTTGCGGCCCATTAGCGGGCATTCCCCGAGCCCCCCGGCA‑TGCACAGGGTTAGGGCCATTTTCAGGGCCGGGGCGGCCCGGGAAGGCCGCGGATGGGGCCCGCACAATTTTACCGGCCGGGACATTTTAcccccccc > 2:69044/1‑149 (MQ=255) gCCGCGTTGCGGCCCATTCGCGGGC‑TTGCCCGCTCCCACAGCCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCa < 3:121152/149‑1 (MQ=255) gCGGCCCATCCGCGGGC‑TTGCCCGCGCCCACCGCCCTTGCACCGGGTCCGCACCTCGTCCAGGACCTGGGCTCGCGGGCAACCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCa < 6:192593/149‑1 (MQ=255) gC‑TTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTg < 4:88228/149‑1 (MQ=255) c‑TTGCCCGCTCCCTCAGGCATTGCACAGGGTGCGGACCTTGTGCAGGTCCTGTGCGAGCGTGCAAGCCCGCGAATGTGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTgg < 2:43128/149‑1 (MQ=255) gCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTACCGGACTGAACATTATCCCCGCCCGAAGGTCAGTTATTCAGGCATAAAACACCTGGGGTTCGt > 6:90969/1‑149 (MQ=39) cacaGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACt > 4:49478/1‑149 (MQ=255) cctGCACAAGGTCCGAACCCTGTGCAATGCCTGTGGGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTga > 1:322497/3‑144 (MQ=25) cctGCACAAGGTCCGAACCCTGTGCAATGCCTGTGGGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTga < 2:322497/142‑1 (MQ=25) gCACAGGGTTCGGACTTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATca < 1:130919/118‑1 (MQ=255) gCACAGGGTTCGGACTTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATca > 2:130919/1‑118 (MQ=255) acaGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTc < 5:45314/108‑1 (MQ=255) acaGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTc > 6:45314/1‑108 (MQ=255) ttCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGgt < 7:252649/120‑1 (MQ=255) ttCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGgt > 8:252649/1‑120 (MQ=255) cTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCAATGAAAGAGt < 3:49478/149‑1 (MQ=255) tCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTcc < 2:101989/124‑1 (MQ=255) tCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTcc > 1:101989/1‑124 (MQ=255) | TATATCCAGACCGCGACCAAGAGCCTGCTGGATAACACCGCCGGCGTGGTGCACGGCACACCGAAGTAAACCGGGGCCGCGTTGCGGCCCATTCGCGGGC‑TTGCCCGCTCCCACAGGCATTGCACAGGGTTCGGACCTTGTGCAGGTCCTGTGCGAGCGGGCAAGCCCGCGAATGGGCCCCGCACAATTTTCCCGGACTGAACATTATCCCCGCCCCAAGGTCAGTTATTCAGGCATCACACACCTGGTGTTCGTTGACTGAGAGGGTCCGTTCAATGAAAGAGT > NC_002947/2063075‑2063359 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |