Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,067,826 T→C 6.6% N77S (AAC→AGC)  plsC ← 1‑acyl‑sn‑glycerol‑3‑phosphate acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,067,8260TC 6.6% 175.6 / 2.7 62N77S (AAC→AGC) plsC1‑acyl‑sn‑glycerol‑3‑phosphate acyltransferase
Reads supporting (aligned to +/- strand):  ref base T (28/29);  new base C (2/2);  total (31/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.18e-01

CGCGCCTGATAGGCATTCTTGCGGTCGACCAGCACGTTGCCGCCCAGCCAGAACAGTTGGCCGAACAGCGGGATCCAGCCCAGGCTCTTCTTGCCGATGGCGACGGTGCGCTGCGGCACCACTTGGCCCAGCACGAACAGATCGAAGTTGGACTGGTGGTTGGCCACGATCACGCAGCCGGGCGGGTGGTCCCACAATGGGCCGACTTCGGCCTTGACCTCGATGCGCATCAGCCAGGTGGCCGGCAAGCTGTAGAGCCGGGCGAAAACGCGGCTGTTGTCAGGGTTGAAGGGGCG  >  NC_002947/2067679‑2067974
                                                                                                                                                   |                                                                                                                                                    
cgcgcCTGATAGGCATTCTTGCGGTCGACCAGCACGTTGCCGCCCAGCCAGAACAGTTGGCCGAACAGCGGGATCCAGCCCATGCTCTTCTTGCCGATGGTGACGGTGCGCTGCTGCACCACTTGGCCCAGCACGAACAGATCGAAGtt                                                                                                                                                     <  1:241110/149‑1 (MQ=255)
   gcCTGATAGGCATTCTTGCGGTCGACCAGCACGTTGCCGCCCAGCCAGAACAGTTGGCCGAACAGCGGGATCCAGCCCAGGCTCTTCTTGCCGATGGCGACGGTGCGCTGCGGCACCACTTGGCCCAGCACGAACAGAGCGAAGTTGGa                                                                                                                                                  >  1:100807/1‑149 (MQ=255)
       gATAGGCATTCTTGCGGTCGACCAGCACGTTGCCGCCCAGCCAGAACAGTTGGCCGAACAGCGGGATCCAGCCCAGGCTCTTCTTGCCGATGGCGACGGTGCGCTGCGGCACCACTTGGCCCAGCACGAACAGATCGAAGTTGGCCtgg                                                                                                                                              >  7:27739/1‑149 (MQ=255)
        aTAGGCATTCTTGCGGTCGACCAGCACGTTGCCGCCCAGCCAGAACAGTTGGCCGAACAGCGGGATCCAGCCCAGGCTCTTCTTGCCGATGGCGACGGTGCGCTGCGGCACCACTTGGCCCAGCACGAACAGATCGAAGTTGGACtggt                                                                                                                                             >  5:89863/1‑149 (MQ=255)
        aTAGGCATTCTTGCGGTCGACCAGCACGTTGCCGCCCAGCCAGAACAGTTGGCCGAACAGCGGGATCCAGCCCAGGCTCTTCTTGCCGATGGCGACGGTGCGCTGCGGCACCACTTGGCCCAGCACGAACAGATCGAAGTTGGACtggt                                                                                                                                             <  8:250592/149‑1 (MQ=255)
        aTAGGCATTCTTGCGGTCGACCAGCACGTTGCCGCCCAGCCAGAACAGTTGGCCGAACAGCGGGATCCAGCCCAGGCTCTTCTTGCCGATGGCGACGGTGCGCTGCGGCACCACTTGGCCCAGCACGAACAGATCGAAGTTGGACtggt                                                                                                                                             >  8:232295/1‑149 (MQ=255)
        aTAGGCATTCTTGCGGTCGACCAGCACGTTGCCGCCCAGCCAGAACAGTTGGCCGAACAGCGGGATCCAGCCCAGGCTCTTCTTGCCGATGGCGACGGTGCGCTGCGGCACCACTTGGCCCAGCACGAACAGATCGAAGTTGGACtg                                                                                                                                               <  6:70513/147‑1 (MQ=255)
            gCATTCTTGCGGTCGACCAGCACGTTGCCGCCCAGCCAGAACAGTTGGCCGAACAGCGGGATCCAGCCCAGGCTCTTCTTGCCGATGGCGACGGTGCGCTGCGGCACCACTTGGCCCAGCACGAACAGATCGAAGTTGGACTGGTGGtt                                                                                                                                         <  7:202111/149‑1 (MQ=255)
              aTTCTTGCGGTCGACCAGCACGTTGCCGCCCAGCCAGAACAGTTGGCCGAACAGCGGGATCCAGCCCAGGCTCTTCTTGCCGATGGCGACGGTGCGCTGCGGCACCACTTGGCCCAGCACGAACAGATCGAAGTTGGACTGGTGGTTgg                                                                                                                                       <  2:148001/149‑1 (MQ=255)
              aTTCTTGCGGTCGACCAGCACGTTGCCGCCCAGCCAGAACAGTTGGCCGAACAGCGGGATCCAGCCCAGGCTCTTCTTGCCGATGGCGACGGTGCGCTGCGGCACCACTTGGCCCAGCACGAACAGATCGAAGTTGGACTGGTGGTTgg                                                                                                                                       <  2:153196/149‑1 (MQ=255)
                    gCGGTCGACCAGCACGTTGCCGCCCAGCCAGAACAGTTGGCCGAACAGCGGGATCCAGCCCAGGCTCTTCTTGCCGATGGCGACGGTGCGCTGCGGCACCACTTGGCCCAGCACGAACAGATCGAAGTTGGACTGGTGGTTGGCCACg                                                                                                                                  <  5:74749/148‑1 (MQ=255)
                    gCGGTCGACCAGCACGTTGCCGCCCAGCCAGAACAGTTGGCCGAACAGCGGGATCCAGCCCAGGCTCTTCTTGCCGATGGCGACGGTGCGCTGCGGCACCACTTGGCCCAGCACGAACAGATCGAAGTTGGACTGGTGGTTGGCCACGa                                                                                                                                 >  8:137210/1‑149 (MQ=255)
                           aCCAGCACGTTGCCGCCCAGCCAGAACAGTTGGCCGAACAGCGGGATCCAGCCCAGGCTCTTCTTGCCGATGGCGACGGTGCGCTGCGGCACCACTTGGCCCAGCACGAACAGATCGAAGTTGGACTGGTGGTTGGCCACGATCACGCa                                                                                                                          <  4:15592/149‑1 (MQ=255)
                                  cGTTGCCGCCCAGCCAGAACAGTTGGCCGAACAGCGGGATCCAGCCCAGGCTCTTCTTGCCGATGGCGACGGTGCGCTGCGGCACCACTTGGCCCAGCACGAACAGATCGAAGTTGGACTGGTGGTTGGCCACGATCACGCAGCcgggc                                                                                                                   <  7:51695/149‑1 (MQ=255)
                                   gTTGCCGCCCAGCCAGAACAGTTGGCCGAACAGCGGGATCCAGCCCAGGCTCTTCTTTCCGATGGCGACGGTGCGCTGCGGCCCCACTTGGCCCAGCACGAACACATCGAAGATGGACTGGAGGTTGGCCACGATCACGCAGCcgcgcg                                                                                                                  >  6:227619/1‑149 (MQ=255)
                                       ccgccCAGCCAGAACAGTTTGCCGAACAGCTGGATCCAGGCCACGCTCTTCTTGCCGATGGCGAGGGTGCGCTGCGGCTCCACTTTGCCCAGCACGATCAGATCGAACTTGCACTGGTGGTTGGCCACGATCACGCAGCcgcgcgggg                                                                                                               <  5:227619/148‑2 (MQ=255)
                                        cgccCAGCCAGAACAGTTGGCCGAACAGCGGGATCCAGCCCAGGCTCTTCTTGCCGATGGCGACGGTGCGCTGCGGCACCACTTGGCCCAGCACGAACAGATCGAAGTTGGACTGGTGGTTGGCCACGATCACGCAGCCGGGCGggtgg                                                                                                             <  7:137210/149‑1 (MQ=255)
                                           ccagccagAACAGTTGGCCGAACAGCGGGATCCAGCCCAGGCTCTTCTTGCCGATGGCGACGGTGCGCTGCGGCACCACTTGGCCCAGCACGAACAGATCGAAGTTGGACTGGTGGTTGGCCACGATCACGCAGCCGGGCGGGTGGTcc                                                                                                          <  1:87356/149‑1 (MQ=255)
                                              gccagAACAGTTGGCCGAACAGCGGGATCCAGCCCAGGCTCTTCTTGCCGATGGCGACGGTGCGCTGCGGCACCACTTGGCCCAGCACGAACAGATCGAAGTTGGACTGGTGGTTGGCCACGATCACGCAGCCGGGCGggtgg                                                                                                             <  5:6905/143‑1 (MQ=255)
                                              gccagAACAGTTGGCCGAACAGCGGGATCCAGCCCAGGCTCTTCTTGCCGATGGCGACGGTGCGCTGCGGCACCACTTGGCCCAGCACGAACAGATCGAAGTTGGACTGGTGGTTGGCCACGATCACGCAGCCGGGCGggtgg                                                                                                             >  6:6905/1‑143 (MQ=255)
                                                  gAACAGTTGGCCGAACAGCGGGATCCAGCCCAGGCTCTTCTTGCCGATGGCGACGGTGCGCTGCGGCACCACTTGGCCCAGCACGAACAGATCGAAGTTGGACTGGTGGTTGGCCACGATCACGCAGCCGGGCGGGTGGTCCCACAATg                                                                                                   >  1:308507/1‑149 (MQ=255)
                                                   aaCAGTTGGCCGAACAGCGGGATCCAGCCCAGGCTCTTCTTGCCGATGGCGACGGTGCGCTGCGGCACCACTTGGCCCAGCACGAACAGATCGAAGTTGGACTGGTGGTTGGCCACGATCACGCAGCCGGGCGGGTGGTCCCACAATgg                                                                                                  >  3:151353/1‑149 (MQ=255)
                                                      aGTTGGCCGAACAGCGGGATCCAGCCCAGGCTCTTCTTGCCGATGGCGACGGTGCGCTGCGGCACCACTTGGCCCAGCACGAACAGATCGAAGTTGGACTGGTGGTTGGCCACGATCACGCAGCCGGGCGGGTGGTCCCACAATGGGcc                                                                                               <  2:322251/149‑1 (MQ=255)
                                                             cGAACAGCGGGATCCAGCCCAGGCTCTTCTTGCCGATGGCGACGGTGCGCTGCGGCACCACTTGGCCCAGCACGAACAGATCGAAGTTGGACTGGTGGTTGGCCACGATCACGCAGCCGGGCGGGTGGTCCCACAATGGGCCGACTTCg                                                                                        >  4:10206/1‑149 (MQ=255)
                                                             cGAACAGCGGGATCCAGCCCAGGCTCTTCTTGCCGATGGCGACGGTGCGCTGCGGCACCACTTGGCCCAGCACGAACAGATCGAAGTTGGACTGGTGGTTGGCCACGATCACGCAGCCGGGCGGGTGGTCCCACAATGGGCCGACTTCg                                                                                        >  6:4515/1‑149 (MQ=255)
                                                             cGAACAGCGGGATCCAGCCCAGGCTCTTCTTGCCGATGGCGACGGTGCGCTGCGGCACCACTTGGCCCAGCACGAACAGATCGAAGTTGGACTGGTGGTTGGCCACGATCACGCAGCCGGGCGGGTGGTCCCACAATGGGCCGACTTCg                                                                                        <  5:124109/149‑1 (MQ=255)
                                                              gAACAGCGGGATCCAGCCCAGGCTCTTCTTGCCGATGGCGACGGTGCGCTGCGGCACCACTTGGCCCAGCACGAACAGATCGAAGTTGGACTGGTGGTTGGCCACGATCACGCAGCCGGGCGGGTGGTCCCACAATGGGCCGACTTCgg                                                                                       >  5:212199/1‑149 (MQ=255)
                                                               aaCAGCGGGATCCAGCCCAGGCTCTTCTTGCCGATGGCGACGGTGCGCTGCGGCACCACTTGGCCCAGCACGAACAGATCGAAGTTGGACTGGTGGTTGGCCACGATCACGCAGCCGGGCGGGTGGTCCCACAATGGGCCGACTTCGGc                                                                                      <  6:212199/149‑1 (MQ=255)
                                                                   gCGGGATCCAGCCCAGGCTCTTCTTGCCGATGGCGACGGTGCGCTGCGGCACCACTTGGCCCAGCACGAACAGATCGAAGTTGGACTGGTGGTTGGCCACGATCACGCAGCCGGGCGGGTGGTCCCACAATGGGCCGACTTCGGCCTTg                                                                                  <  7:284190/149‑1 (MQ=255)
                                                                    cGGGATCCAGCCCAGGCTCTTCTTGCCGATGGCGACGGTGCGCTGCGGCACCACTTGGCCCAGCACGAACAGATCGAAGTTGGACTGGTGGTTGGCCACGATCACGCAGCCGGGCGGGTGGTCCCACAATGGGCCGACTTCGGCCTTGa                                                                                 >  4:296371/1‑149 (MQ=255)
                                                                     gggATCCAGCCCAGGCTCTTCTTGCCGATGGCGACGGTGCGCTGCGGCACCACTTGGCCCAGCACGAACAGATCGAAGTTGGACTGGTGGTTGGCCACGATCACGCAGCCGGGCGGGTGGTCCCACAATGGGCCGACTTCGGCCTTGAc                                                                                <  7:232295/149‑1 (MQ=255)
                                                                     gggATCCAGCCCAGGCTCTTCTTGCCGATGGCGACGGTGCGCTGCGGCACCACTTGGCCCAGCACGAACAGATCGAAGTTGGACTGGTGGTTGGCCACGATCACGCAGCCGGGCGGGTGGTCCCACAATGGGCCGACTTCGGCCTTGAc                                                                                >  5:33572/1‑149 (MQ=255)
                                                                         tCCAGCCCAGGCTCTTCTTGCCGATGGCGACGGTGCGCTGCGGCACCACTTGGCCCAGCACGACCAGATCGAAGTTGGACTGGTGGTTGGCCACGATCACGCAGCCGGGCGGGTGGTCCCACAATGGGCCGACTTCGGCCTTGCCCTCg                                                                            >  4:210064/1‑149 (MQ=255)
                                                                            aGCCCAGGCTCTTCTTGCCGATGGCGACGGTGCGCTGCGGCACCACTTGGCCCAGCACGAACAGATCGAAGTTGGACTGGTGGTTGGCCACGATCACGCAGCCGGGCGGGTGGTCCCACAATGGGCCGACTTCGGCCTTGACCt                                                                              >  3:305318/1‑144 (MQ=255)
                                                                            aGCCCAGGCTCTTCTTGCCGATGGCGACGGTGCGCTGCGGCACCACTTGGCCCAGCACGAACAGATCGAAGTTGGACTGGTGGTTGGCCACGATCACGCAGCCGGGCGGGTGGTCCCACAATGGGCCGACTTCGGCCTTGACCt                                                                              <  4:305318/144‑1 (MQ=255)
                                                                            aGCCCAGGCTCTTCTTGCCGATGGCGACGGTGCGCTGCGGCACCACTTGGCCCAGCACGAACAGATCGAAGTTGGACTGGTGGTTGGCCACGATCACGCAGCCGGGCGGGTGGTCCCACAATGGGCCGACTTCGGCCTTGACCTCGATg                                                                         >  1:133993/1‑149 (MQ=255)
                                                                              cccAGGCTCTTCTTGCCGATGGCGACGGTGCGCTGCGGCACCACTTGGCCCAGCACGAACAGATCGAAGTTGGACTGGTGGTTGGCCACGATCACGCAGCCGGGCGGGTGGTCCCACAATGGGCCGACTTCGGCCTTGACCTCGATgcg                                                                       >  5:77159/1‑149 (MQ=255)
                                                                                     gcgtcttGCCGATGGCGACGGTGCGCTGCGGCACCACTTGTCCCAGCACGAACAGAGCGAAGTTGGACTGGTGTTTGGCCACGATCACGCAGCCGGGCGGGTGGACCCACAATGGGCCGACTTCGGCCTTGACCTCGATGCGCATCAGc                                                                <  4:206586/146‑1 (MQ=255)
                                                                                         cttGCCGATGGCGACGGTGCGCTGCGGCACCACTTGGCCCAGCACGAACAGATCGAAGTTGGACTGGTGGTTGGCCACGATCACGCAGCCGGGCGGGTGGTCCCACAATGGGCCGACTTCGGCCTTGACCTCGATGCGCATCAGCCa                                                              <  3:296371/147‑1 (MQ=255)
                                                                                             ccGATGGCGACGGTGCGGTGCGGCAACACTTGGCCCATCACGAACAGATCGTAGTTGGACTGGGGGTTGGCCACGATCACGGAGCCGGGCGGGTGGTCCCACAATGGGCCGACTTCGGCCTTGACCTCGATGCGCAt                                                                    <  3:255304/137‑1 (MQ=255)
                                                                                             ccGATGGCGACGGTGCGCTGCGGCACCACTTGGCCCAGCACGAACAGATCGAAGTTGGACTGGTGGTTGGCCACGATCACGCAGCCGGGCGGGTGGTCCCACAATGGGCCCACTACGGCCTTCACCACTATgcgaaa                                                                    >  4:255304/1‑134 (MQ=255)
                                                                                              cGATGGCGACGGTGCGCTGCGGCACCACTTGGCCCAGCACGAACAGATCGAAGTTGGACTGGTGGTTGGCCACGATCACGCAGCCGGGCGGGTGGTCCCACAATGGGCCGACTTCGGCCTTGACCTCGATGCGCATCAGCCAGGTGGcc                                                       <  5:4515/149‑1 (MQ=255)
                                                                                              cGATGGCGACGGTGCGCTGCGGCACCACTTGGCCCAGCACGAACAGATCGAAGTTGGACTGGTGGTTGGCCACGATCACGCAGCCGGGCGGGTGGTCCCACAATGGGCCGACTTCGGCCTTGACCTAGATGCGCATCAGCCAGGTGTcc                                                       >  5:62074/1‑149 (MQ=255)
                                                                                               gATGGCGACGGTGCGCTGCGGCACCACTTGGCCCAGCACGAACAGATCGAAGTTGGACTGGTGGTTGGCCACGATCACGCAGCCGGGCGGGTGGTCCCACAATGGGCCGACTTCGGCCTTGACCTCGATGCGCATCAGCCAGGTGGCCg                                                      <  2:308507/149‑1 (MQ=255)
                                                                                                aTGGCGACGGTGCGCTGCGGCACCACTTGGCCCAGCACGAACAGATCGAAGTTGGACTGGTGGTTGGCCACGATCACGCAGCCGGGCGGGTGGTCCCACAATGGGCCGACTTCGGCCTTGACCTCGATGCGCATCAGCCAGGTGGCCgg                                                     >  6:175822/1‑149 (MQ=255)
                                                                                                     gACGGTGCGCTGCGGCACCACTTGGCCCAGCACGAACAGATCGAAGTTGGACTGGTGGTTGGCCACGATCACGCAGCCGGGCGGGTGGTCCCACAATGGGCCGACTTCGGCCTTg                                                                                  >  4:116841/1‑115 (MQ=255)
                                                                                                       cGGTGCGCTGCGTCACCACTTGGCCAAGCACGAACAGATCGAAGTTGGACTGGTGGTTGGCCACGATCACGCAGCCGGGCGGGTGGTCCCACAATGGGCCGACTTCGTCCTTGACCTCGATGCGCATCAGCCAGGTGGCCGGCAAGCTg                                              <  2:100807/149‑1 (MQ=255)
                                                                                                                 cGGCACCACTTGGCCCAGCACGAACAGATCGAAGTTGGACTGGTGGTTGGCCACGATCACGCAGCCGGGCGGGTGGTCCCACAATGGGCCGACTTCGGCCTTGACCTCGATGCGCATCAGCCAGGTGGCCGGCAAGCTGTAGAGCCgg                                     <  4:151353/148‑1 (MQ=255)
                                                                                                                    caccacTTGGCCCAGCACGAACAGATCGAAGTTGGACTGGTGGTTGGCCACGATCACGCAGCCGGGCGGGTGGTCCCACAATGGGCCGACTTCGGCCTTGACCTCGATGCGCATCAGCCAGGTGGCCGGCAAGCTGTAGAGCCGGGCg                                  >  8:295146/1‑148 (MQ=255)
                                                                                                                    cacaacTTGGCCCAGCACGAACAGATCGAAGTTGGACTGGTGGTTGGCCACGATCACGCAGCCGGGAGGGTGGTCCCACAATGGGCCTACTTCGTCCTTGACCTCGATGATCATCAGCCAGGTGGCCGGCAAGCTGTAGAGCCGGGCg                                  >  2:271399/1‑148 (MQ=255)
                                                                                                                             gCCCAGCACGAACAGATCGAAGTTGGACTGGTGGTTGGCCACGATCACGCAGCCGGGCGGGTGGTCCCACAATGGGCCGACTTCGGCCTTGACCTCGATGCGCATCAGCCAGGTGGCCGGCAAGCTGTAGAGCCGGGCGAAAACGCgg                         <  6:33572/148‑1 (MQ=255)
                                                                                                                             gCCCAGCACGAACAGATCGAAGTTGGACTGGTGGTTGGCCACGATCACGCAGCCGGGCGGGTGGTCCCACAATGGGCCGACTTCGGCCTTGACCTCGATGCGCATCAGCCAGGTGGCCGGCAAGCTGTAGAGCCGGGCGAAAACGCGGc                        >  2:60056/1‑149 (MQ=255)
                                                                                                                              cccAGCACGAACAGATCGAAGTTGGACTGGTGGTTGGCCACGATCACGCAGCCGGGCGGGTGGTCCCACAATGGGCCGACTTCGGCCTTGACCTCGATGCGCATCAGCCAGGTGGCCGGCAAGCTGTAGAGCCGGGCGAAAACGCGGCt                       <  8:27739/149‑1 (MQ=255)
                                                                                                                              cccAGCACGAACAGATCGAAGTTGGACTGGTGGTTGGCCACGATCACGCAGCCGGGCGGGTGGTCCCACAATGGGCCGACTTCGGCCTTGACCTCGATGCGCATCAGCCAGGTGGCCGGCAAGCTGTAGAGCCGGGCGAAAACGCGGCt                       >  2:78360/1‑149 (MQ=255)
                                                                                                                               ccAGCACGAACAGATCGAAGTTGGACTGGTGGTTGGCCACGATCACGCAGCCGGGCGGGTGGTCCCACAATGGGCCGACTTCGGCCTTGACCTCGATGCGCATCAGCCAGGTGGCCGGCAAGCTGt                                             >  3:2619/1‑126 (MQ=255)
                                                                                                                               ccAGCACGAACAGATCGAAGTTGGACTGGTGGTTGGCCACGATCACGCAGCCGGGCGGGTGGTCCCACAATGGGCCGACTTCGGCCTTGACCTCGATGCGCATCAGCCAGGTGGCCGGCAAGCTGt                                             <  4:2619/126‑1 (MQ=255)
                                                                                                                               ccAGCACGAACAGATCGAAGTTGGACTGGTGGTTGGCCACGATCACGCAGCCGGGCGGGTGGTCCCACAATGGGCCGACTTCGGCCTTGACCTCGATGCGCATCAGCCAGGTGGCCGGCAAGCTGTAGAGCCGGGCGAAAACGCGGCtg                      >  6:327725/1‑149 (MQ=255)
                                                                                                                               ccAGCACGAACAGATCGAAGCTGGACTGGTGGTTGGCCACGATCACGCAGCCGGGCGGGTGGTCCCACAATGGGCCGACTTCGGCCTTGACCTCGATGCGCATCAGCCAGGTGGCCGGCAAGCTGTAGAGCCGGGCGAAAACGCGGCtg                      >  1:324650/1‑149 (MQ=255)
                                                                                                                               ccAGCACGAACAGATCGAAGCTGGACTGGTGGTTGGCCACGATCACGCAGCCGGGCGGGTGGTCCCACAATGGGCCGACTTCGGCCTTGACCTCGATGCGCATCAGCCAGGTGGCCGGCAAGCTGTAGAGCCGGGCGAAAACGCGGCtg                      >  1:319513/1‑149 (MQ=255)
                                                                                                                                 aGCACGAACAGATCGAAGCTGGACTGGTGGTTGGCCACGATCACGCAGCCGGGCGGGTGGTCCCACAATGGGCCGACTTCGGCCTTGACCTCGATGCGCATCAGCCAGGTGGCCGGCAAGCTGTAGAGCCGGGCGAAAACGCGGCtgtt                    <  2:319513/149‑1 (MQ=255)
                                                                                                                                 aGCACGAACAGATCGAAGCTGGACTGGTGGTTGGCCACGATCACGCAGCCGGGCGGGTGGTCCCACAATGGGCCGACTTCGGCCTTGACCTCGATGCGCATCAGCCAGGTGGCCGGCAAGCTGTAGAGCCGGGCGAAAACGCGGCtgtt                    <  2:324650/149‑1 (MQ=255)
                                                                                                                                       aaCAGATCGAAGTTGGACTGGTGGTTGGCCACGATCCCGCAGCCGGGCGGGTGGTCCCACAATGGGCCGACTTCGGCCTTGACCTCGATGCGCATCAGCCAGGTGGCCGGCAAGCTGTAGAGCCGGGCGAAAACGCGGCTGTTGTCAgg              <  5:327725/149‑1 (MQ=255)
                                                                                                                                                aaGTTGGACTGGTGGTTGGCCACGATCACGCAGCCGGGCGGGTGGTCCCTCAATGGGCCGACTTCGGCCTTGACCTCGATGCGCATCAGCCAGGTGGCCGGCAAGCTGTAGAGCCGTGCGAAAACGCGGCTGTTGTCAGGGTTGAAggg     >  4:131183/1‑149 (MQ=255)
                                                                                                                                                   ttGGACTGGTGGTTGGCCACGATCACGCAGCCGGGCGGGTGGTCCCACAATGGGCCGACTTCGGCCTTGACCTCGATGCGCATCAGCCAGCTGGCCGGCAAGCTGTAGAGCCGGGCGAAAACGCGGCTGTTGTCAGGGTTGAAGGggcg  >  7:35661/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CGCGCCTGATAGGCATTCTTGCGGTCGACCAGCACGTTGCCGCCCAGCCAGAACAGTTGGCCGAACAGCGGGATCCAGCCCAGGCTCTTCTTGCCGATGGCGACGGTGCGCTGCGGCACCACTTGGCCCAGCACGAACAGATCGAAGTTGGACTGGTGGTTGGCCACGATCACGCAGCCGGGCGGGTGGTCCCACAATGGGCCGACTTCGGCCTTGACCTCGATGCGCATCAGCCAGGTGGCCGGCAAGCTGTAGAGCCGGGCGAAAACGCGGCTGTTGTCAGGGTTGAAGGGGCG  >  NC_002947/2067679‑2067974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑