Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,082,906 G→C 7.7% L110V (CTG→GTG)  PP_1861 ← LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,082,9060GC 7.7% 203.3 / 7.0 65L110V (CTG→GTG) PP_1861LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (29/31);  new base C (2/3);  total (31/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

GCGGGTGTCGGCCAAGGGGCCGGCGCGCAGCACCAGGTCGACCTCGCCCAGGTGGCTGCCCTGCAGGTCGACAAAGCTGTCGATCAACCGCAGTTGCACGTCCAGTCCCGGGTAGGCCACCAGGAAGT‑CGGCAATGGCCGGGGCCAGGTGGCGGCGGCCAAACGCGG‑CAGGGGCGTCGATGCGGATCAGGCCTTCCGGGGCATTGCTCAGTGACACCGCTTCAGCCCGGGCCAGGCGCAACTCCTCGATGATGCGCCTGGCCCGCTCGGCAAAGGCGTTGCCTGCAGGCGTGGC  >  NC_002947/2082760‑2083053
                                                                                                                                                   |                                                                                                                                                    
gCGGGTGTCGGCCAAGGGGCCGGCGCGCAGCACCAGGTCGACCTCGCCCAGGTGGCTGCCCTGCAGGTCGACAAAGCTGTCGATCAACCGCAGTTGCACGTCCAGTCCCGGGTAGGCCACCAGGAAGT‑CGGCAATGGCCGGGGCCAGGt                                                                                                                                                    >  6:324668/1‑149 (MQ=255)
     tgtCGGCCAAGGGGCCGGCGCGCAGCACCAGGTCGACCTCGCCCAGGTGGCTGCCCTGCAGGTCGACAAAGCTGTCGATCAACCGCAGTTGCACGTCCAGTCCCGGGTAGGCCACCAGGAAGT‑CGGCAATGGCCGGGGCCAGGTggcgg                                                                                                                                               >  1:60965/1‑149 (MQ=255)
        cGGCCAAGGGGCCGGCGCGCAGCACCAGGTCGACCTCGCCCAGGTGGCTGCCCTGCAGGTCGACAAAGCTGTCGATCAACCGCAGTTGCACGTCCAGTCCCGGGTAGGCCACCAGGAAGT‑CGGCAATGGCCGGGGCCAGGTggcggcgg                                                                                                                                            <  6:114181/149‑1 (MQ=255)
         ggCCAAGGGGCCGGCGCGCAGCACCAGGTCGACCTCGCCCAGGTGGCTGCCCTGCAGGTCGACAAAGCTGTCGATCAACCGCAGTTGCACGTCCAGTCCCGGGTAGGCCACCAGGAAGT‑CGGCAATGGCCGGGGCCAGGTggcggcggc                                                                                                                                           <  5:80530/149‑1 (MQ=255)
            cAAGGGGCCGGCGCGCAGCACCAGGTCGACCTCGCCCAGGTGGCTGCCCTGCAGGTCGACAAAGCTGTCGATCAACCGCAGTTGCACGTCCAGTCCCGGGTAGGCCACCAGGAAGT‑CGGCAATGGCCGCCGCCACCTggccccggc                                                                                                                                           >  5:68678/1‑146 (MQ=255)
            cAAGGGGCCGGCGCGCAGCACCAGGTCGACCTCGCCCAGGTGGCTGCCCTGCAGGTCGACAAAGCTGTCGATCAACCGCAGTTGCACGTCCAGTCCCGGGTAGGCCACCAGGAAGT‑CGGCAATGGCCGCCGCCACCTggccccggc                                                                                                                                           <  6:68678/146‑1 (MQ=255)
                   gcGGCGCGCAGCACCAGGTCGACCTCGCCCAGGTGGCTGCCCTGCAGGTCGACAAAGCTGTCGATCAACCGCAGTTGCACGTCCAGTCCCGGGTAGGCCACCAGGAAGT‑CGGCAATGGCCGGGGCCAGGTGGCGGCGGCCAAACGCGG‑c                                                                                                                                <  8:325364/148‑1 (MQ=255)
                             ccaCCTGGGCGAGGTCGACCTGGTGGCTGCCCTGCAGGTCGACAAAGCTGTCGATCAACCGCAGTTGCACGTCCAGTCCCGGGTAGGCCACCAGGAAGT‑CGGCAATGGCCGGGGCCAGGTGGCGGCGGCCAAACGCGG‑CAGGGGCGTc                                                                                                                       >  6:294451/2‑148 (MQ=255)
                                ccAGGTCGACCTCGCCCAGGTGGCTGCCCTGCAGGTCGACAAAGCTGTCGATCAACCGCAGTTGCACGTCCAGTCCCGGGTAGGCCACCAGGAAGT‑CGGCAATGGCCGGGGCCAGGTGGCGGCGGCCAAACGCGG‑CAGGGGCGTCGATg                                                                                                                   >  8:28539/1‑149 (MQ=255)
                                 cAGGTCGACCTCGCCCAGGTGGCTGCCCTGCAGGTCGACAAAGCTGTCGATCAACCGCAGTTGCACGTCCAGTCCCGGGTAGGCCACCAGGAAGT‑CGGCAATGGCCGGGGCCAGGTGGCGGCGGCCAAACGCGG‑CAGGGGCGt                                                                                                                        <  5:104874/143‑1 (MQ=255)
                                 cAGGTCGACCTCGCCCAGGTGGCTGCCCTGCAGGTCGACAAAGCTGTCGATCAACCGCAGTTGCACGTCCAGTCCCGGGTAGGCCACCAGGAAGT‑CGGCAATGGCCGGGGCCAGGTGGCGGCGGCCAAACGCGG‑CAGGGGCGt                                                                                                                        >  6:104874/1‑143 (MQ=255)
                                  aGGTCGACCTCGCCCAGGTGGCTGCCCTGCAGGTCGACAAAGCTGTCGATCAACCGCAGTTGCACGTCCAGTCCCGGGTAGGCCACCAGGAAGT‑CGGCAATGGCCGGGGCCAGGTGGCGGCGGCCAAACGCGG‑CAGGGGCGTCGATGCg                                                                                                                 <  2:60965/149‑1 (MQ=255)
                                        aCCTCGCCCAGGTGGCTGCCCTGCAGGTCGACAAAGCTGTCGATCAACCGCAGTTGCACGTCCAGTCCCGGGTAGGCCACCAGGAAGT‑CGGCAATGGCCGGGGCCAGGTGGCGGCGGCCAAAcgc                                                                                                                                    <  3:131651/125‑1 (MQ=255)
                                        aCCTCGCCCAGGTGGCTGCCCTGCAGGTCGACAAAGCTGTCGATCAACCGCAGTTGCACGTCCAGTCCCGGGTAGGCCACCAGGAAGT‑CGGCAATGGCCGGGGCCAGGTGGCGGCGGCCAAAcgc                                                                                                                                    >  4:131651/1‑125 (MQ=255)
                                        aCCTCGCCCAGGTGGCTGCCCTGCAGGTCGACAAAGCTGTCGATCAACCGCAGTTGCACGTCCAGTCCCGGGTAGGCCACCAGGAAGT‑CGGCAATGGCCGGGGCCAGGTGGCGGCGGCCAAACGCGG‑CAGGGGCGTCGATGCGGATCAg                                                                                                           <  5:271851/149‑1 (MQ=255)
                                        aCCTCGCCCAGGTGGCTGCCCTGCAGGTCGACAAAGCTGTCGATCAACCGCAGTTGCACGTCCAGTCCCGGGTAGGCCACCAGGAAGT‑CGGCAATGGCCGCCGCCACCTGGCCCCGGCCatt                                                                                                                                       <  2:3637/122‑3 (MQ=255)
                                        aCCTCGCCCAGGTGGCTGCCCTGCAGGTCGACAAAGCTGTCGATCAACCGCAGTTGCACGTCCAGTCCCGGGTAGGCCACCAGGAAGT‑CGGCAATGGCCGCCGCCACCTGGCCCCGGCCatt                                                                                                                                       >  1:3637/1‑120 (MQ=255)
                                         gctggCCCATGTGGCTGCCCTGCAGGTCGACAAAGCTGTCGATCAACCGCAGTTGCACGTCCAGTCCCGGGTAGGCCACCAGGAAGT‑CGGCAATGGCCGGGGCCAGGTGGCGGCGGCCAAACGCGG‑Ca                                                                                                                               <  4:64051/124‑1 (MQ=255)
                                         ccTCGCCCAGGTGGCTGCCCTGCAGGTCGACAAAGCTGTCGATCAACCGCAGTTGCACTTCCAGTCCCGGGTAGGCCAGCAGGAAGT‑CGGCAATGGCCGGGGCCAGGTGGCGGCGGCCAAACGCGG‑CAGGGGCGTCGATGCGGATCAgg                                                                                                          <  6:25698/149‑1 (MQ=255)
                                         ccTCGCCCAGGTGGCTGCCCTGCAGGTCGACAAAGCTGTCGATCAACCGCAGTTGCACGTCCAGTCCCGGGTAGGCCACCAGGAAGT‑CGGCAATGGCCGGGGCCAGGTGGCGGCGGCCAAACGCGG‑CAGGGGCGTCGATGCGGATCAgg                                                                                                          <  4:320634/149‑1 (MQ=255)
                                                      gCTGCCCTGCAGGTCGACAAAGCTGTCGATCAACCGCAGTTGCACGTCCAGTCCCGGGTAGGCCACCAGGAAGT‑CGGCAATGGCCGGGGCCAGGTGGCGGCGGCCAAACGCGG‑CAGGGGCGTCGATGCGGATCAGGCCTTCCGGGGCAt                                                                                             >  5:295758/1‑149 (MQ=255)
                                                          cccTGCAGGTCGACAAAGCTGTCGATCAACCGCAGTTGCACGTCCAGTCCCGGGTAGGCCACCAGGAAGT‑CGGCAATGGCCGGGGCCAGGTGGCGGCGGCCAAACGCGG‑CAGGGGCGTCGATGCGGATCAGGCCTTCCGGGGCATTg                                                                                           <  3:84773/147‑1 (MQ=255)
                                                             tGCAGGTCGACAAAGCTGTCGATCAACCGCAGTTGCACGTCCAGTCCCGGGTAGGTCACCAGGAAGT‑CGGCAATGGCCGGGGCCAGGTGGCGGCGGCCAAACGCGG‑CAGGGGCGTCGATGCGGATCAGGCCTTCCGGGGCATTGCTCAg                                                                                      >  8:266527/1‑149 (MQ=255)
                                                               cAGGTCGACAAAGCTGTCGATCAACCGCAGTTGCACGTCCAGTCCCGGGTAGGTCACCAGGAAGT‑CGGCAATGGCCGGGGCCAGGTGGCGGCGGCCAAACGCGG‑CAGGGGCGTCGATGCGGATCAGGCCTTCCGGGGCATTGCTCAGt                                                                                     <  7:266527/148‑1 (MQ=255)
                                                               cAGGTCGACAAAGCTGTCGATCAACCGCAGTTGCACGTCCAGTCCCGGGTAGGCCACCAGGAAGT‑CGGCAATGGCCGGGGCCAGGTGGCGGCGGCCAAACGCGGCCAGGGGCGTCGATGCGGATCAGGCCTTCCGGGGCATTGCTCAGt                                                                                     <  1:245398/149‑1 (MQ=255)
                                                               cAGGTCGACAAAGCTGTCGATCAACCGCAGTTGCACGTCCAGTCCCGGGTAGGCCACCAGGAAGT‑CGGCAATGGCCGGGGCCAGGTGGCGGCGGCCAAACGCGG‑CAGGGGCGTCGATGCGGATCAGGCCTTCCGGGGCATTGCTCAGt                                                                                     <  8:34367/148‑1 (MQ=255)
                                                               cAGGTCGACAAAGCTGTCGATCAACCGCAGTTGCACGTCCAGTCCCGGGTAGGCCACCAGGAAGT‑CGGCAATGGCCGGGGCCAGGTGGCGGCGGCCAAACGCGG‑CAGGGGCGTCGATGCGGATCAGGCCTTCCGGGGCATTGCTCAGt                                                                                     <  5:324668/148‑1 (MQ=255)
                                                                  gTCGACAAAGCTGTCGATCAACCGCAGTTGCACGTCCAGTCCCGGGTAGGCCACCAGGAAGT‑CGGCAATGGCCGGGGCCAGGTGGCGGCGGCCAAACGCGG‑CAGGGGCGTCGATGCGGATCAGGCCTTCCGGGGCATTGCTCAGTGaca                                                                                 <  7:28539/149‑1 (MQ=255)
                                                                  gTCGACAAAGCTGTCGATCAACCGCAGTTGCACGTCCAGTCCCGGGTAGGCCACCAGGAAGT‑CGGCAATGGCCGGGGCCAGGTGGCGGCGGCCAAACGCGG‑CAGGGGCGTCGATGCGGATCAGGCCTTCCGGGGCATTGCTCAGTGaca                                                                                 >  6:122315/1‑149 (MQ=255)
                                                                             tGTCGATCAACCGCAGTTGCACGTCCAGTCCCGGGTAGGCCACCAGGAAGTACGGCAATGGCCGGGGCCAGGTGGCGGCGGCCAAACGCGG‑CAGGGGCGTCGATGCGGATCAGGCCTTCCGGGGCATTGCTCAGTGACACCGCTTCAGc                                                                       >  1:259567/1‑149 (MQ=255)
                                                                              gTCGATCAACCGCAGTTGCACGTCCAGTCCCGGGTAGGCCACCAGGAAGT‑CGGCAATGGCCGGGGCCAGGTGGCGGCGGCCAAACGCGG‑CAGGGGCGTCGATGCGGATCAGGCCTTCCGGGGCATTGCTCAGTGACACCGCTTCAGccc                                                                     >  8:67527/1‑149 (MQ=255)
                                                                                   tCAACCGCAGTTGCACGTCCAGTCCCGGGTAGGCCACCAGGAAGT‑CGGCAATGGCCGGGGCCAGGTGGCGGCGGCCAAACGCGG‑CAGGGGCGTCGATGCGGATCAGGCCTTCCGGGGCATTGCTCAGTGACACCGCTTCAGCCCGGGcc                                                                >  2:82814/1‑149 (MQ=255)
                                                                                      aCCGCAGTTGCACGTCCAGTCCCGGGTAGGCCACCAGGAAGT‑CGGCAATGGCCGGGGCCAGGTGGCGGCGGCCAAACGCGG‑CAGGGGCGTCGCTGCGGATCAGGCCTTCCGGGGACTTGCTCAGTGACACCGCTTCAGCCCGGGCCAgg                                                             >  7:61543/1‑149 (MQ=255)
                                                                                       ccGCAGTTGCACGTCCAGTCCCTGGTAGGCCACCAGGAAGT‑CGGCAATGGCCGGGGCCAGGTGGCGGCGGCCAAACGCGG‑CAGGGGCGTCGATGCGGATCAGGCCTTCCGGGGCATTGCTCAGTGACACCGc                                                                             <  7:237564/132‑1 (MQ=255)
                                                                                       ccGCAGTTGCACGTCCAGTCCCGGGTAGGCCACCAGGAAGT‑CGGCAATGGCCGGGGCCAGGTGGCGGCGGCCAAACGCGG‑CAGGGGCGTCGATGCGGATCAGGCCTTCCGGGGCATTGCTCAGTGACACCGCTTCAGCCCGGGCCAGgc                                                            >  4:122253/1‑149 (MQ=255)
                                                                                       ccGCAGTTGCACGTCCAGTCCCGGGTAGGCCACCAGGAAGT‑CGGCAATGGCCGGGGCCAGGTGGCGGCGGCCAAACGCGG‑CAGGGGCGTCGATGCGGATCAGGCCTTCCGGGGCATTGCTCAGTGACACCGCTTCAGCCCGGGCCAGgc                                                            >  3:23212/1‑149 (MQ=255)
                                                                                       ccGCAGTTGCACGTCCAGTCCCGGGTAGGCCACCAGGAAGT‑CGGCAATGGCCGGGGCCAGGTGGCGGCGGCCAAACGCGG‑CAGGGGCGTCGAGGCGGATCAGGCCTGCCGGGGCCTTGCTCAGTGACACCGc                                                                             >  8:237564/1‑132 (MQ=255)
                                                                                          cAGTTGCACGTCCAGTCCCGGGTAGGCCACCAGGAAGT‑CGGCAATGGCCGGGGCCAGGTGGCGGCGGCCAAACGCGG‑CAGGGGCGTCGATGCGGATCAGGCCTTCCGGGGCAt                                                                                             >  7:323445/1‑113 (MQ=255)
                                                                                          cAGTTGCACGTCCAGTCCCGGGTAGGCCACCAGGAAGT‑CGGCAATGGCCGGGGCCAGGTGGCGGCGGCCAAACGCGG‑CAGGGGCGTCGATGCGGATCAGGCCTTCCGGGGCAt                                                                                             <  8:323445/113‑1 (MQ=255)
                                                                                          cAGTTGCACGTCCAGTCCCGGGTAGGCCACCAGGAAGT‑CGGCAATGGCCGGGGCCAGGTGGCGGCGGCCAAACGCGG‑CAGGGGCGTCGATGCGGATCAGGCCTTCCGGGGCATTGCTCAGTGACACCGCTTCAGCCCGGGCCAGgcgc                                                          >  5:80458/1‑148 (MQ=255)
                                                                                               gCACGTCCAGTCCCGGGTAGGCCACCAGGAAGT‑CGGCAATGGCCGGGGCCAGGTGGCGGCGGCCAAACGCGG‑CAGGGGCGTCGATGCGGATCAGGCCTTCCGGGGCATTGCTCAGTGACACCGCTTCAGCCCGGGCCAGGCGCatcatc                                                    >  2:197809/1‑144 (MQ=255)
                                                                                                   gTCCAGTCCCGGGTAGGCCACCAGGAAGT‑CGGCAATGGCCGGGGCCAGGTGGCGGCGGCCAAACGCGG‑CAGGGGCGTCGATGCGGATCAGGCCTTcc                                                                                                    <  7:28379/97‑1 (MQ=255)
                                                                                                   gTCCAGTCCCGGGTAGGCCACCAGGAAGT‑CGGCAATGGCCGGGGCCAGGTGGCGGCGGCCAAACGCGG‑CAGGGGCGTCGATGCGGATCAGGCCTTcc                                                                                                    >  8:28379/1‑97 (MQ=255)
                                                                                                         tCCCGGGTAGGCCACCAGGAAGT‑CGGCAATGGCCGGGGCCAGGTGGCGGCGGCCAAACGCGG‑CAGGGGCGTCGATGCGGATCAGGCCTTCCGGGGCATTGCTCAGTGACACCGCTTCAGCCCGGGCCAGGCGCAACTCCTCGATGATgc                                          >  2:189775/1‑149 (MQ=255)
                                                                                                         tCCCGGGTAGGCCACCAGGAAGT‑CGGCAATGGCCGGGGCCAGGTGGCGGCGGCCAAACGCGG‑CAGGGGCGTCGATGCGGATCAGGCCTTCCGGGGCATTGCTCAGTGACACCGCTTCAGCCCGGGCCAGGCGCAACTCCTCGATGATgc                                          >  4:55883/1‑149 (MQ=255)
                                                                                                           ccGGGTAGGCCACCAGGAAGT‑CGGCAATGGCCGGGGCCAGGTGGCGGCGGCCAAACGCGG‑CAGGGGCGTCGATGCGGATCAGGCCTTCCGGGGCATTGCt                                                                                         <  6:64459/100‑1 (MQ=255)
                                                                                                           ccGGGTAGGCCACCAGGAAGT‑CGGCAATGGCCGGGGCCAGGTGGCGGCGGCCAAACGCGG‑CAGGGGCGTCGATGCGGATCAGGCCTTCCGGGGCATTGCt                                                                                         >  5:64459/1‑100 (MQ=255)
                                                                                                              ggTAGGCCACCAGGAAGT‑CGGCAATGGCCGGGGCCAGGTGGCGGCGGCCAAACGCGG‑CAGGGGCGTCGATGCGGATCAGGCCTTCCGGGGCATTGCTCAGTGACACCGCTTCAGCCCGGGCCAGGCGCAACTCCTCGATGATGCGCCTg                                     >  7:130502/1‑149 (MQ=255)
                                                                                                              ggTAGGCCACCAGGAAGT‑CGGCAATGGCCGGGGCCAGGTGGCGGCGGCCAAACGCGG‑CAGGGGCGTCGATGCGGATCAGGCCTTCCGGGGCATTGCTCAGTGACACCGCTTCAGCCCGGGCCAGGCGCAACTCCTCGATGATGCGCCTg                                     <  8:197675/149‑1 (MQ=255)
                                                                                                                tAGGCCACCAGGAAGT‑CGGCAATGGCCGGGGCCAGGTGGCGGCGGCCAAACGCGG‑CAGGGGCGTCGATGCGGATCAGGCCTTCCGGGGCATTGCTCAGTGACACCGCTTCAGCCCGGGCCAGGCGCAACTCCTCGATGATGCGCCTGGc                                   <  5:122315/149‑1 (MQ=255)
                                                                                                                    ccaccaGGAAGT‑CGGCAATGGCCGGGGCCAGGTGGCGGCGGCCAAACGCGG‑CAGGGGCGTCGATGCGGATCAGGCCTTCCGGGGCATTGCTCAGTGACACCGCTTCAGCCCGGGCCAGGCGCAACTCCTCGATGATGCGCCTGGCCCGc                               >  4:196921/1‑149 (MQ=255)
                                                                                                                       ccaGGAAGT‑CGGCAATGGCCGGGGCCAGGTGGCGGCGGCCAAACGCGG‑CAGGGGCGTCGATGCGGATCAGGCCTTCCGGGGCATTGCTCAGTGACACCGCTTCAGCCCGGGCCAGGCGCAACTCCTCGATGATGCGCCTGGCCCGCTCg                            >  3:59980/1‑149 (MQ=255)
                                                                                                                             aGT‑CGGCAAGGGCCGGGGCCAGGGGGCGGCGGCAAAACGCGG‑CAGGGGCGTCGATGCGGATCAGGCCTTCCGGGGCATTGCTCAGTCACACCGCTTCAGCCCGGGCCAGGCGCAACTCCTCGATGATGCGCCTGGCCCGCTCGGCAAAg                      <  8:130502/149‑1 (MQ=255)
                                                                                                                              gT‑CGGCAATGGCCGGGGCCAGGTGGCGGCGGCCAAACGCGG‑CAGGGGCGTCGATGCGGATCAGGCCTTCCGGGGCATTGCTCAGTGACACCGCTTCAGCCCGGGCCAGGCGCAACTCCTCGATGATGCGCCTGGCCCGCTCGGCAAAgg                     >  3:106444/1‑149 (MQ=255)
                                                                                                                                  gcggttGGCCGCCGCCACCTGGCCCCGGCCAAACGCGG‑CAGGGGCGTCGATGCGGATCAGGCCTTCCGGGGCATTGCTCAGTGACACCGCTTCAGCCCGGGCCAGGCGCAACTCCTCGATGATGCGCCTGGCCCGCTCGGCAAAGGc                    <  6:263621/142‑1 (MQ=255)
                                                                                                                                   gCAATGGCCGGGGCCAGGTGGCGGCGGCCAAACGCGG‑CAGGGGCGTCGATGCGGATCAGGCCTTCCGGGGCATTGCTCAGTGACACCGCTTCAGCCCGGGCCAGGCGCAACTCCTCGATGATGCGCCTGGCCCGCTCGGCAAAGGCGtt                 <  7:67527/149‑1 (MQ=255)
                                                                                                                                      aTGGCCGGGGCCAGGTGGCGGCGGCCAAACGCGG‑CAGGGGCGTCGATGCGGATCAGGCCTTCCGGGGCATTGCTCAGTGACACCGCTTCAGCCCGGGCCAGGCGCAACTCCTCGATGATGCGCCTGGCCCGCTCGGCAAAGGCGTTGcc              <  4:106444/149‑1 (MQ=255)
                                                                                                                                      aTGGCCGGGGCCAGGTGGCGGCGGCCAAACGCGG‑CAGGGGCGTCGATGCGGATCAGGCCTTCCGGGGCATTGCTCAGTGACACCGCTTCAGCCCGGGCCAGGCGCAACTCCTCGATGATGCGCCTGGCCCGCTCGGCAAAGGCGNTGcc              <  5:294451/149‑1 (MQ=255)
                                                                                                                                        ggCCGGGGCCAGGTGGCGGCGGCCAAACGCGG‑CAGGGGCGTCGATGCGGATCAGGCCTTCCGGGGCATTGCTCAGTGACACCGCTTCAGCCCGGGCCAGGCGCAACTCCTCGATGATGCGCCTGGCCCGCTCGGCAAAGGCGTTGCCTg            >  5:235561/1‑149 (MQ=255)
                                                                                                                                        ggCCGGGGCCAGGTGGAGGCGGCCAAACGCGG‑CAGGGGCGTCGATGCGGATCAGGCCTTCCGGGGCATTGCTCAGTGACACCGCTTCAGCCCGGGCCAGGCGCCACTCCTCGATGATGCGCCTGGCCCGCTCGGCAAAGGCGTTGCCTg            >  6:67591/1‑149 (MQ=255)
                                                                                                                                             gggCCAGGTGGCGGCGGCCAAACGCGG‑CAGGGGCGTCGATGCGGATCAGGCCTTCCGGGGCATTGCTCAGTGACACCGCTTCAGCCCGGGCCAGGCGCAACTCCTCGATGATGCGCCTGGCCCGCTCGGCAAAGGCGTTGCCTGCAGGc       <  1:82814/149‑1 (MQ=255)
                                                                                                                                                ccAGGTGGCGGCGGCCAAACGCGG‑CAGGGGCGTCGATGCGGCTCAGGCCATCCGGGGCATTGCTCAGTGACACCGCGTCAGCCCGGGCCAGGCGCAACTCCTCGATGATGCGCCTGGCCCGCTCGGCAAAGGCGTTGCCTGCAGGCGTg    <  8:61543/149‑1 (MQ=255)
                                                                                                                                                ccAGGTGGCGGCGGCCAAACGCGG‑CAGGGGCGTCGATGCGGATCAGGCCTTCCGGGGCATTGCTCAGTGACACCGCTTCAGCCCGGGCCAGGCGCAACTCCTCGATGATGCGCCTGGCCCGCTCGGCAAAGGCGTTGCCTGCAGGCGTg    <  6:80458/149‑1 (MQ=255)
                                                                                                                                                ccAGGTGGCGGCGGCCAAACGCGG‑CAGGGGCGTCGATGCGGATCAGGCCTTCCGGGGCATTGCTCAGTGACACCGCTTCAGCCCGGGCCAGGCGCAACTCCTCGATGATGCGCCTGGCCCGCTCGGCAAAGGCGTTGCCTGCAGGCGTg    <  4:59980/149‑1 (MQ=255)
                                                                                                                                                  aGGTGGCGGCGGCCAAACGCGG‑CAGGGGCGTCGATGCGGATCAGGCCTTCCGGGGCATTGCTCAGTGACACCGCTTCAGCCCGGGCCAGGCGCAACTCCTCGATGATGCGCCTGGCCCGCTCGGCAAAGGCGTTGCCTGCAGGCGTGGc  <  1:189775/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GCGGGTGTCGGCCAAGGGGCCGGCGCGCAGCACCAGGTCGACCTCGCCCAGGTGGCTGCCCTGCAGGTCGACAAAGCTGTCGATCAACCGCAGTTGCACGTCCAGTCCCGGGTAGGCCACCAGGAAGT‑CGGCAATGGCCGGGGCCAGGTGGCGGCGGCCAAACGCGG‑CAGGGGCGTCGATGCGGATCAGGCCTTCCGGGGCATTGCTCAGTGACACCGCTTCAGCCCGGGCCAGGCGCAACTCCTCGATGATGCGCCTGGCCCGCTCGGCAAAGGCGTTGCCTGCAGGCGTGGC  >  NC_002947/2082760‑2083053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑