| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,166,707 | C→A | 10.2% | intergenic (‑551/‑230) | PP_5483 ← / → PP_1921 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,166,707 | 0 | C | A | 10.2% | 146.8 / 6.9 | 49 | intergenic (‑551/‑230) | PP_5483/PP_1921 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (21/23); new base A (2/3); total (23/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01 | |||||||||||
CCCTCGATCAGAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCAGGAGAGAGGAGGGTTGGTGTTATTTTGCTACTATGACTATGCGCACGACGGGCG > NC_002947/2166560‑2166854 | cccTCGATCAGAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCt > 2:45014/1‑149 (MQ=255) aTCAGAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAgg < 3:154514/149‑1 (MQ=255) gAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGc < 8:165156/149‑1 (MQ=255) aaGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAg < 1:45014/149‑1 (MQ=255) aaGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGCATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGGCGCTGGGTGAGTGAAGCTCAATCAACCTGGAAGCCAGGTTgatt < 6:269592/149‑4 (MQ=255) ctctCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGATACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgta < 1:281324/149‑1 (MQ=255) tctcAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGAAGCCAGGTTgattgag < 4:298934/145‑7 (MQ=255) tctcAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGAAGCCAGGTTgattgag > 3:298934/1‑139 (MQ=255) tcAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgtagg > 1:313627/1‑148 (MQ=255) tcAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgtag < 2:4684/147‑1 (MQ=255) aGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgtagg < 5:151193/146‑1 (MQ=255) gCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgtagggagt < 6:323793/149‑1 (MQ=255) aaCCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgtagggagtag < 2:313627/149‑1 (MQ=255) aaCCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgtagggagtag > 4:150525/1‑149 (MQ=255) gCAGAGTGTTTAAGGATCGCCTTTGGCCATTATGTGAAACTACCCGCTCCTGGAGCGGTATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGATTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGTTCgtagggagtagtgagtag < 8:203290/148‑1 (MQ=255) cAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgtagggagtagggagtagg < 6:235285/148‑1 (MQ=255) cAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgtagggagtagggagtagg < 3:150525/148‑1 (MQ=255) cAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgtagggagtagggagtagg > 5:235285/1‑148 (MQ=255) tAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgtagggagtagggagtagggagtaggga < 8:43780/148‑1 (MQ=255) tAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgtagggagtagggagtagggagtaggga < 1:115770/148‑1 (MQ=255) tCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAgggg > 8:79851/1‑149 (MQ=255) tGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGga < 8:6129/148‑1 (MQ=255) aTGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGgcg > 1:232352/1‑149 (MQ=255) gtgAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGcgcg > 7:279627/1‑149 (MQ=255) ccGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGgc > 4:310298/1‑136 (MQ=255) ccGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGgc < 3:310298/136‑1 (MQ=255) gAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAg > 7:294047/1‑116 (MQ=255) gatgatCCGAGGGCTGTGGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCa < 2:232352/149‑1 (MQ=255) atgatCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGCAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCAg < 1:45993/149‑1 (MQ=255) gAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCAGGagagag < 7:79851/149‑1 (MQ=255) gggCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGa < 4:172120/131‑1 (MQ=255) gggCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGa > 3:172120/1‑131 (MQ=255) tGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCAGGAGAGAGGAGGGt > 2:104830/1‑149 (MQ=255) gtgtCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCAGGAGAGAGGAGGGTTGGTGtt > 6:304171/1‑149 (MQ=255) tgtCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgtagggagtagggagtagggagta > 8:225698/1‑74 (MQ=255) tgtCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgtagggagtagggagtagggagta < 7:225698/74‑1 (MQ=255) gCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCAGGAGAGAGGAGGGTTGGTGTTAtttt > 4:102772/1‑149 (MQ=255) caatcaaCCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCAGGAGAGAGGAGGGTTGGTGTTATTTTGCTACTATGACTATgcg > 1:127387/1‑149 (MQ=255) ctgcaaCCTGGAAGCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCAGGAGAGAGGAGGGTTGGTGTTAtt > 7:91016/4‑129 (MQ=255) ctgcaaCCTGGAAGCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCAGGAGAGAGGAGGGTTGGTGTTAtt < 8:91016/126‑1 (MQ=255) aatcaaCCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCAGGAGAGAGGAGGGTTGGTGTTATTTTGcta < 2:284562/135‑1 (MQ=255) aatcaaCCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCAGGAGAGAGGAGGGTTGGTGTTATTTTGcta > 1:284562/1‑135 (MQ=255) atcaaCCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTAGAGCGAAGCAGGAGAGAGGAGGGTTGGTGTTATTTTGCTACTATGACTATGCGCa > 5:262613/1‑149 (MQ=255) caaCCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCAGGAGAGAGGAGGGTTGGTGTTATTTTGCTAATATGACTATgcgc > 7:137553/1‑146 (MQ=255) aCCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCAGGAGAGAGGAGGGTTGGTGTTATTTTGctacta < 4:239933/133‑1 (MQ=255) aCCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCAGGAGAGAGGAGGGTTGGTGTTATTTTGctacta > 3:239933/1‑133 (MQ=255) ccTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCAGGAGAGAGGAGGGTTGGTGTTATTTTGCTACTATGACTATGCGCacgacg > 5:156539/1‑149 (MQ=255) gCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGGAGATTCTGTCGAGCGAAGCAGGAGAGAGGAGGGTTGGTGTTTTTTTGCTACTATGACTATGCGCACGACGGGCg > 8:4005/1‑149 (MQ=255) gCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCAGGAGAGAGGAGGGTTGGTGTTATTTTGCTACTATGACTATGCGCACGACGGGCg < 6:156539/149‑1 (MQ=255) | CCCTCGATCAGAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCAGGAGAGAGGAGGGTTGGTGTTATTTTGCTACTATGACTATGCGCACGACGGGCG > NC_002947/2166560‑2166854 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |