Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,166,709 T→G 10.4% intergenic (‑553/‑228) PP_5483 ← / → PP_1921 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,166,7090TG10.4% 136.6 / 7.7 48intergenic (‑553/‑228)PP_5483/PP_1921hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (20/23);  new base G (2/3);  total (22/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

ATCAGAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCAGGAGAGAGGAGGGTTGGTGTTATTTTGCTACTATGACTATGCGCACGACGGGCGGT  >  NC_002947/2166566‑2166856
                                                                                                                                               |                                                                                                                                                   
aTCAGAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAgg                                                                                                                                                <  3:154514/149‑1 (MQ=255)
    gAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGc                                                                                                                                            <  8:165156/149‑1 (MQ=255)
      aaGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAg                                                                                                                                          <  1:45014/149‑1 (MQ=255)
      aaGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGCATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGGCGCTGGGTGAGTGAAGCTCAATCAACCTGGAAGCCAGGTTgatt                                                                                                                                          <  6:269592/149‑4 (MQ=255)
            ctctCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGATACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgta                                                                                                                                    <  1:281324/149‑1 (MQ=255)
             tctcAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGAAGCCAGGTTgattgag                                                                                                                                       <  4:298934/145‑7 (MQ=255)
             tctcAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGAAGCCAGGTTgattgag                                                                                                                                       >  3:298934/1‑139 (MQ=255)
               tcAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgtagg                                                                                                                                  >  1:313627/1‑148 (MQ=255)
               tcAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgtag                                                                                                                                   <  2:4684/147‑1 (MQ=255)
                 aGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgtagg                                                                                                                                  <  5:151193/146‑1 (MQ=255)
                  gCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgtagggagt                                                                                                                              <  6:323793/149‑1 (MQ=255)
                    aaCCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgtagggagtag                                                                                                                            >  4:150525/1‑149 (MQ=255)
                    aaCCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgtagggagtag                                                                                                                            <  2:313627/149‑1 (MQ=255)
                            gCAGAGTGTTTAAGGATCGCCTTTGGCCATTATGTGAAACTACCCGCTCCTGGAGCGGTATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGATTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGTTCgtagggagtagtgagtag                                                                                                                     <  8:203290/148‑1 (MQ=255)
                             cAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgtagggagtagggagtagg                                                                                                                    <  3:150525/148‑1 (MQ=255)
                             cAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgtagggagtagggagtagg                                                                                                                    <  6:235285/148‑1 (MQ=255)
                             cAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgtagggagtagggagtagg                                                                                                                    >  5:235285/1‑148 (MQ=255)
                                      tAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgtagggagtagggagtagggagtaggga                                                                                                           <  8:43780/148‑1 (MQ=255)
                                      tAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgtagggagtagggagtagggagtaggga                                                                                                           <  1:115770/148‑1 (MQ=255)
                                            tCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAgggg                                                                                                    >  8:79851/1‑149 (MQ=255)
                                                   tGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGga                                                                                              <  8:6129/148‑1 (MQ=255)
                                                           aTGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGgcg                                                                                     >  1:232352/1‑149 (MQ=255)
                                                             gtgAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGcgcg                                                                                   >  7:279627/1‑149 (MQ=255)
                                                                       ccGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGgc                                                                                      <  3:310298/136‑1 (MQ=255)
                                                                       ccGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGgc                                                                                      >  4:310298/1‑136 (MQ=255)
                                                                                gAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAg                                                                                                 >  7:294047/1‑116 (MQ=255)
                                                                                      gatgatCCGAGGGCTGTGGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCa                                                          <  2:232352/149‑1 (MQ=255)
                                                                                       atgatCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGCAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCAg                                                         <  1:45993/149‑1 (MQ=255)
                                                                                              gAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCAGGagagag                                                  <  7:79851/149‑1 (MQ=255)
                                                                                                gggCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGa                                                                  <  4:172120/131‑1 (MQ=255)
                                                                                                gggCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGa                                                                  >  3:172120/1‑131 (MQ=255)
                                                                                                    tGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCAGGAGAGAGGAGGGt                                            >  2:104830/1‑149 (MQ=255)
                                                                                                           gtgtCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCAGGAGAGAGGAGGGTTGGTGtt                                     >  6:304171/1‑149 (MQ=255)
                                                                                                            tgtCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgtagggagtagggagtagggagta                                                                                                               <  7:225698/74‑1 (MQ=255)
                                                                                                            tgtCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCgtagggagtagggagtagggagta                                                                                                               >  8:225698/1‑74 (MQ=255)
                                                                                                                gCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCAGGAGAGAGGAGGGTTGGTGTTAtttt                                >  4:102772/1‑149 (MQ=255)
                                                                                                                                 caatcaaCCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCAGGAGAGAGGAGGGTTGGTGTTATTTTGCTACTATGACTATgcg               >  1:127387/1‑149 (MQ=255)
                                                                                                                                  ctgcaaCCTGGAAGCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCAGGAGAGAGGAGGGTTGGTGTTAtt                                  >  7:91016/4‑129 (MQ=255)
                                                                                                                                  ctgcaaCCTGGAAGCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCAGGAGAGAGGAGGGTTGGTGTTAtt                                  <  8:91016/126‑1 (MQ=255)
                                                                                                                                  aatcaaCCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCAGGAGAGAGGAGGGTTGGTGTTATTTTGcta                            <  2:284562/135‑1 (MQ=255)
                                                                                                                                  aatcaaCCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCAGGAGAGAGGAGGGTTGGTGTTATTTTGcta                            >  1:284562/1‑135 (MQ=255)
                                                                                                                                   atcaaCCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTAGAGCGAAGCAGGAGAGAGGAGGGTTGGTGTTATTTTGCTACTATGACTATGCGCa             >  5:262613/1‑149 (MQ=255)
                                                                                                                                     caaCCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCAGGAGAGAGGAGGGTTGGTGTTATTTTGCTAATATGACTATgcgc              >  7:137553/1‑146 (MQ=255)
                                                                                                                                       aCCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCAGGAGAGAGGAGGGTTGGTGTTATTTTGctacta                         <  4:239933/133‑1 (MQ=255)
                                                                                                                                       aCCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCAGGAGAGAGGAGGGTTGGTGTTATTTTGctacta                         >  3:239933/1‑133 (MQ=255)
                                                                                                                                        ccTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCAGGAGAGAGGAGGGTTGGTGTTATTTTGCTACTATGACTATGCGCacgacg        >  5:156539/1‑149 (MQ=255)
                                                                                                                                            gggtCCATGTTGCATGGCGTTGGGATTTGGGCGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCAGGAGAGAGGAGGGGTGGTGTTATTTTGCTACTATGACTATGCGCACGACGGGc     <  3:254822/145‑1 (MQ=255)
                                                                                                                                            gCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGGAGATTCTGTCGAGCGAAGCAGGAGAGAGGAGGGTTGGTGTTTTTTTGCTACTATGACTATGCGCACGACGGGCg    >  8:4005/1‑149 (MQ=255)
                                                                                                                                            gCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCAGGAGAGAGGAGGGTTGGTGTTATTTTGCTACTATGACTATGCGCACGACGGGCg    <  6:156539/149‑1 (MQ=255)
                                                                                                                                              ttCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCAAGAGAGAGGAGGCTTGCTGTTATTTTGCTAATATGACAATGCGCACGACGGGCgct  >  1:95889/1‑147 (MQ=255)
                                                                                                                                              ttCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGGGCAGATTGTGTCGAGCGAAGCAGGAGTGAGGAGGGTTGGTGTTATTTTGCTACTATGACTTTGCGCACGACGGGCGGt  >  4:19786/1‑149 (MQ=255)
                                                                                                                                               tCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCAGGAGAGAGGAGGGTTGGTGTTATTTTGCTACTATGACTATGCGCACGACGGGCGGt  <  8:137553/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
ATCAGAAAGTTACTCTCAGCAACCTATTGCAGAGGGTTTAAGGATCGCCGTTGGCCATTATGTGAAACGACCCGCTCCTGGAGCGGGATGATCCGAGGGCTGTTGGGGTGTCGCTGGTTGAGTGAAGCTCAATCAACCTGGCTTCCAGGTTGCAGGTCGTAGGGAGTAGGGAGTAGGGAGTAGGGAGTAGGGGCAGGGAGAGTCGGCGCGGGTGCAGATTCTGTCGAGCGAAGCAGGAGAGAGGAGGGTTGGTGTTATTTTGCTACTATGACTATGCGCACGACGGGCGGT  >  NC_002947/2166566‑2166856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑