| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 197,695 | G→C | 8.2% | T1067T (ACG→ACC) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 197,695 | 0 | G | C | 8.2% | 189.4 / 5.5 | 60 | T1067T (ACG→ACC) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base G (26/29); new base C (2/3); total (28/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
AACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACG > NC_002947/197548‑197843 | aaCCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGc < 1:310152/149‑1 (MQ=35) aCCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGcc > 6:300839/1‑149 (MQ=37) aCCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGcc > 6:25608/1‑149 (MQ=37) aTTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGc < 3:213179/149‑1 (MQ=37) ccGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCa < 4:147097/148‑1 (MQ=12) cGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGa > 6:275768/1‑149 (MQ=37) gTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGAc > 4:72465/1‑149 (MQ=37) aaGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCAc < 5:320244/148‑1 (MQ=37) aaGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACg < 2:330061/149‑1 (MQ=37) gCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGAt > 4:31138/1‑149 (MQ=12) ggCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAc > 7:31099/1‑149 (MQ=38) gCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATCaac < 5:167186/149‑3 (MQ=2) aCCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTc > 4:256369/1‑149 (MQ=39) cccAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCg < 5:25608/149‑1 (MQ=39) ccAGGGCAGCGTCGATTTCCAGACACCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCgg > 1:270742/1‑149 (MQ=255) aGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTc < 7:96732/149‑1 (MQ=39) aTTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAc < 2:205851/127‑1 (MQ=39) cccGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGa > 2:1012/1‑149 (MQ=255) ccGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGAc < 7:299696/149‑1 (MQ=255) cGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCaa < 3:72465/149‑1 (MQ=255) tCTACAACAACGGTTCGACTGTCAGCGTCAACATCGAGATCGCCACAGGCGGTAATTTCGAGCAACTTACCCCTAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGc < 4:230851/149‑1 (MQ=255) tactacAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGTCAGCACTACTGCGAc < 3:74607/128‑1 (MQ=255) aacaacaACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATATCGAGCAACTGACCCCGAACCCGACGCCGCCTCAGACCACGATCACCCACTCGGTCGACACCACTACTGCGAc > 4:74607/2‑128 (MQ=255) acaacaACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGt > 4:78200/1‑148 (MQ=255) acaacaACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGt > 8:103462/1‑148 (MQ=255) aacaacGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGAcc < 4:86002/126‑1 (MQ=40) aacaacGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGAcc > 3:86002/1‑126 (MQ=40) caacGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGAcc > 6:21816/1‑148 (MQ=255) aacGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCg > 8:310463/1‑148 (MQ=255) acGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGaa < 5:21816/149‑1 (MQ=255) cGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAg > 2:326889/1‑149 (MQ=255) ttCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAggcg > 7:18017/1‑149 (MQ=255) ttCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAggcg > 7:16543/1‑149 (MQ=255) gACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGgcggcg < 1:1012/149‑1 (MQ=255) tCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATc < 3:256369/149‑1 (MQ=255) tCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATc < 1:173784/149‑1 (MQ=255) tCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATc < 8:31099/149‑1 (MQ=255) aGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCAc < 5:275768/149‑1 (MQ=255) gTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTg > 8:136592/1‑141 (MQ=255) gTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTg < 7:136592/141‑1 (MQ=255) cACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGAcc > 4:161705/1‑109 (MQ=40) cACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGAcc < 3:161705/109‑1 (MQ=40) cATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCg > 7:42135/1‑149 (MQ=255) cATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCg > 3:171161/1‑149 (MQ=14) tCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGt > 1:126631/1‑148 (MQ=255) tCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGt > 5:16982/1‑148 (MQ=255) gagaACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGa < 4:171161/148‑1 (MQ=14) cGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGa < 5:300839/149‑1 (MQ=255) cGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGa < 8:18017/149‑1 (MQ=255) cGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGa < 2:126631/149‑1 (MQ=255) ccACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGc < 8:16543/149‑1 (MQ=255) cacaGGCGGTAATTTCGAGCAACTGCCCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGc > 7:58508/1‑148 (MQ=255) gTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGcc < 7:310463/149‑1 (MQ=255) aTTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCa < 6:16982/148‑1 (MQ=255) aaCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGt > 3:134102/1‑148 (MQ=255) aaCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGt > 2:50718/1‑148 (MQ=255) cTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAAc < 8:42135/149‑1 (MQ=255) ccGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGAc < 3:78200/149‑1 (MQ=255) gACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTaa < 2:238106/134‑1 (MQ=255) gACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTaa > 1:238106/1‑134 (MQ=255) gCCGGCTCAGACCACGCTCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACg < 2:270742/149‑1 (MQ=255) | AACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACG > NC_002947/197548‑197843 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |