| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,211,904 | C→T | 7.4% | D12D (GAC→GAT) | PP_1955 → | cytochrome P450 family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,211,904 | 0 | C | T | 7.4% | 156.8 / 2.0 | 55 | D12D (GAC→GAT) | PP_1955 | cytochrome P450 family protein |
| Reads supporting (aligned to +/- strand): ref base C (27/23); new base T (2/2); total (30/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.25e-01 | |||||||||||
GTGGCCTCTGCGGCACTCTAAGTTAAATATAGATTTCCTCAGCGTCATGTAGTGATACTTACTTGGTGGGTAGCCGCGTGCCTGA‑GGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAGCGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAACCCCTGTCTTTTTCTATGCGCCGCTAGGCGTGTGGATGGTAACGCGTCGAGAAGATGCCGAGCGCGTTTTGAGCGAGTGG > NC_002947/2211756‑2212051 | gtggCCTCTGCGGCACTCTAAGTTAAATATAGATTTCCTCAGCGTCATGTAGTGATACTTACTTGGTGGGTAGCCGCGTGCCTGA‑GGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAGCGCCATCGGAc < 3:121891/149‑1 (MQ=255)gtggCCTCTGCGGCACTCTAAGTTAAATATAGATTTCCTCAGCGTCATGTAGTGATACTTACTTGGTGGGTAGCCGCGTGCCTGA‑GGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAGCGCCATCGGAc < 5:354405/149‑1 (MQ=255)gtggCCTCTGCGGCACTCTAAGTTAAATATACATTTCCTCAGCGTCATGTAGTCATACTTACTTCCTGCGTAGCCGCGTGCCTGA‑GGAAATAATTGTTCCGAGAATACCAAAAATGGAAATACTTCATCGAGCTCAAGCGCCATCGGAc > 5:48969/1‑149 (MQ=255) gCCTCTGCGGCACTCTAAGTTAAATATAGATTTCCTCAGCGTCATGTAGTGATACTTACTTGGTGGGTAGCCGCGTGCCTGA‑GGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAGCGCCATCGGACtt < 3:62629/148‑1 (MQ=255) tGCGGCACTCTAAGTTAAATATAGATTTCCTCAGCGTCATGTAGTGATACTTACTTGGTGGGTAGCCGCGTGCCTGA‑GGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAGCGCCATCGGACTTCAATcc > 7:320914/1‑149 (MQ=255) cGGCACTCTAAGTTAAATATAGATTTCCTCAGCGTCATGTAGTGATACTTACTTGGTGGGTAGCCGCGTGCCTGA‑GGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAGCGCCATCGGACTTCAATc < 2:112870/146‑1 (MQ=255) cGGCACTCTAAGTTAAATATAGATTTCCTCAGCGTCATGTAGTGATACTTACTTGGTGGGTAGCCGCGTGCCTGA‑GGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAGCGCCATCGGACTTCAATCCGa > 5:179905/1‑149 (MQ=255) cACTCTAAGTTAAATATAGATTTCCTCAGCGTCATGTAGTGATACTTACTTGGTGGGTAGCCGCGTGCCTGA‑GGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAGCGCCATCGGATTGAAGTCCGAtgg < 1:21328/149‑2 (MQ=255) aCTCTAAGTTAAATATAGATTTCCTCAGCGTCATGTAGTGATACTTACTTGGTGGGTAGCCGCGTGCCTGA‑GGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAGCGCCATCGGACTTCAATCCGAtgag < 2:246398/149‑1 (MQ=255) aGTTAAATATAGATTTCCTCAGCGTCATGTAGTGATACTTACTTGGTGGGTAGCCGCGTGCCTGA‑GGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAGCGCCATCGGATTGAAGTCCGAtggcgct < 2:175923/146‑6 (MQ=255) aGTTAAATATAGATTTCCTCAGCGTCATGTAGTGATACTTACTTGGTGGGTAGCCGCGTGCCTGA‑GGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAGCGCCATCGGACTTCAATCCGATGAGTGAGCa < 3:118780/149‑1 (MQ=255) aaTATAGATTTCCTCAGCGTCATGTAGTGATACTTACTTGGTGGGTAGCCGCGTGCCTGA‑GGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAGCGCCATCGGACTTCAATCCGAt > 2:16566/1‑135 (MQ=255) aaTATAGATTTCCTCAGCGTCATGTAGTGATACTTACTTGGTGGGTAGCCGCGTGCCTGA‑GGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAGCGCCATCGGACTTCAATCCGAt < 1:16566/135‑1 (MQ=255) taGATTTCCTCAGCGTCATGTAGTGATACTTACTTGGTGGGTAGCCGCGTGCCTGA‑GGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAGCGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCg < 6:179905/149‑1 (MQ=255) aTTTCCTCAGCGTCATGTAGTGATACTTACTTGGTGGGTAGCCGCGTGCCTGA‑GGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAGCGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGa > 4:278946/1‑149 (MQ=255) cTCAGCGTCATGTAGTGATACTTACTTGGTGGGTAGCCGCGTGCCTGA‑GGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAGCGCCATCGGACTTCAATCCGAt < 2:243070/123‑1 (MQ=255) cTCAGCGTCATGTAGTGATACTTACTTGGTGGGTAGCCGCGTGCCAGA‑GGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAGCGCCATCGGACTTCAATCCGAt > 1:243070/1‑123 (MQ=255) tCAGCGTCATGTAGTGATACTTACTTGGTGGGTAGCCGCGAGCCTGA‑GGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAGCGCCATCGGACTTCAATCCGATCAGTGAGCAGAGTTTTCGTGATCCAg > 7:42051/1‑148 (MQ=255) aGCGTCATGTAGTGATACTTACTTGGTGGGTAGCCGCGAGCCTGA‑GGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAGCGCCATCGGACTTCAATCCGATCAGTGAGCAGAGTTTTCGTGATCCAg < 8:42051/146‑1 (MQ=255) cGTCATGTAGTGATACTTACTTGGTGGGTAGCCGCGTGCCTGA‑GGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCACAAGCGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGAACCAGCCTc > 8:226730/1‑148 (MQ=255) gTAGTGATACTTACTTGGTGGGTAGCCGCGTGCCTGA‑GGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAGCGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCg > 2:111076/1‑130 (MQ=255) gTAGTGATACTTACTTGGTGGGTAGCCGCGTGCCTGA‑GGAAATAATTGTTCGCAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAGCGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCg < 1:111076/130‑1 (MQ=255) gATACTTACTTGGTGGGTAGCCGCGTGCCTGA‑GGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAGCGCCATCGGACTTCAATCCGATGAGTGACCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCg > 5:148173/1‑149 (MQ=255) aTACTTACTTGGTGGGTAGCCGCGTGCCTGA‑GGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAGCGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGt > 5:158921/1‑149 (MQ=255) aTACTTACTTGGTGGGTAGCCGCGTGCATGAGGGAAATAATTGTTCGGAGAATAACAAAAATGAAAATACTTGAACGTCCTCAACCGCCATCGCAATTCAATCCGATGAGTGAGAAGAGTTTTCATGATCCAGAATAAAACTGCCACCg > 1:210661/1‑149 (MQ=255) acttGGTGGGTAGCCGCGTGCCTGA‑GGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAGCGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTgcgc < 2:156986/147‑1 (MQ=255) tggGTAGCCGCGTGCCTGA‑GGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAGCGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCgaaga > 7:201431/1‑148 (MQ=255) tgTAGCCGCGTGCCTGA‑GGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAGCGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGaaa < 7:233471/147‑1 (MQ=255) gTAGCCGCGTGCCTGA‑GGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAGCGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAAcc < 3:278946/149‑1 (MQ=255) gCCGCGTGCCTGA‑GGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAGCGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAACCCCt < 6:208733/149‑1 (MQ=255) cgcgTGCCTGA‑GGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAGCGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAACCCCTGt > 3:226139/1‑149 (MQ=255) gA‑GGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAGCGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGaaa < 2:290233/133‑1 (MQ=255) gA‑GGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAGCGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGaaa > 1:290233/1‑133 (MQ=255) a‑GGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAGCGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAACCCCTGTCTTTTTCt < 4:188279/147‑1 (MQ=255) ggAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAGCGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAACCCCTGTCTTTTTCTATg > 2:330637/1‑149 (MQ=255) aatTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAGCGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAACCCCTGTCTTTTTCTATgcgc > 3:117595/1‑146 (MQ=255) aatTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAGCGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAACCCCTGTCTTTTTCTATgcgc < 4:117595/146‑1 (MQ=255) atTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAGCGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTgcgcgc < 4:279560/117‑1 (MQ=255) atTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAGCGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTgcgcgc > 3:279560/1‑117 (MQ=255) ttGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAGTGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAACCCCTGTCTTTTTCTATGcgccgc > 1:245283/1‑147 (MQ=255) gagaATAACAAAAATGGAAATACTTGATCGTCCTCAAGCGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAACCCCTGTCTTTTTCTATGCGCCGCTAGGCgtgt > 7:61422/1‑149 (MQ=255) gagaATAACAAAAATGGAAATACTTGATCGTCCTCAAGCGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAACCCCTGTCTTTTTCTATGCGCCGCTAGGCgtgt < 1:330637/149‑1 (MQ=255) aaCAAAAATGGAAATACTTGATCGTCCTCAAGCGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAACCCCTGTCTTTTTCTATGCGCCGCTAGGCGTGTGGATgg > 5:131220/1‑149 (MQ=255) aaaaaTGGAAATACTTGATCGTCCTCAATCGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAACCCCTGTCTTTTTCTATGCGCCGCTAGGCGTGTGGATGGTaa < 6:148173/149‑1 (MQ=255) aaaaTGGAAATACTTGATCGTCCTCAAGCGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAACCCCTGTCTTTTTCTATGCGCCGCTAGGCGTGTGGATGGTAAc > 3:253250/1‑149 (MQ=255) aTGGAAATACTTGATCGTCCTCAAGCGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAACCCCTGTCTTTTTCTATGCGCCGCTAGGCGTGTGGATGGTAAcgcg > 4:144661/1‑149 (MQ=255) ggAAATACTTGATCGTCCTCAAGCGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAACCCCTGTCTTTTTCTATGCGCCGCTAGGCGTGTGGATGGTAACGCGTc > 1:226626/1‑149 (MQ=255) ggAAATACTTGATCGTCCTCAAGCGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAACCCCTGTCTTTTTCTATGCGCCGCTAGGCGTGTGGATGGTAACGCGTc > 3:327392/1‑149 (MQ=255) tACTTGATCGTCCTCAAGCGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAACCCCTGTCTTTTTCTATGCGCCGCTAGGCGTGTGGATGGTAACGCGTCGagaa < 6:158921/149‑1 (MQ=255) aTCGTCCTCAAGCGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAACCCCTGTCTTTTTCTATGCGCCGCTAGGCGTGTGGATGGTAACGCGTCGAGAAGATGcc > 5:47108/1‑149 (MQ=255) tCGTCCTCAAGCGCCATCGGACTTCAATCCGATGAGTGAGCAGAGATTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAACCCCTGTCTTTTTCTATGCGCCGCTAGGCGTGTGGATGGTAACGCGTCCAGAAGATGcc > 3:230460/1‑148 (MQ=255) aaGCGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAACCCCTGTCTTTTTCTATGCGCCGCTAGGCGTGTGGATGGTAACGCGTCGAGAAGATGCCGAGcgcg < 4:226139/147‑1 (MQ=255) aGCGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAGCCCCTGTCTTTTTCTATGCGCCGCTAGGCGTGTGGATGGTAACGCGTCGAGAAGATGCCGAGCGCGttt > 5:331277/1‑149 (MQ=255) tcATCGGATTGAAGTCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAACCCCTGTCTTTTTCTATGCGCCGCTAGGCGTGTGGATGGTAACGCGTCGAGAAGATGCCGAGCGCGtttt > 8:173167/2‑146 (MQ=255) tcATCGGATTGAAGTCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAACCCCTGTCTTTTTCTATGCGCCGCTAGGCGTGTGGATGGTAACGCGTCGAGAAGATGCCGAGCGCGTTTTGAg > 1:173635/2‑149 (MQ=255) tCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAACCCCTGTCTTTTTCTATGCGCCGGTAGGCGTGTGGATGGTAACGCGTCGAGAAGATGCCGAGCGCGTTTTGAGCGa < 2:226626/149‑1 (MQ=255) tCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAACCCCTGTCTTTTTCTATGCGCCGCTAGGCGTGTGGATGGTAACGCGTCGAGAAGATGCCGAGCGCGTTTTGAGc > 3:241891/1‑147 (MQ=255) aCTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAACCCCTGTCTTTTTCTATGCGCCGCTAGGCGTGTGGATGGTAACGCGGCGAGAAGATGCCGAGCGCGTTTTGAGCGAGTgg < 2:245283/149‑1 (MQ=255) | GTGGCCTCTGCGGCACTCTAAGTTAAATATAGATTTCCTCAGCGTCATGTAGTGATACTTACTTGGTGGGTAGCCGCGTGCCTGA‑GGAAATAATTGTTCGGAGAATAACAAAAATGGAAATACTTGATCGTCCTCAAGCGCCATCGGACTTCAATCCGATGAGTGAGCAGAGTTTTCGTGATCCAGCCTCAATCTGCCAGCGTGCGCGCGAAGAAACCCCTGTCTTTTTCTATGCGCCGCTAGGCGTGTGGATGGTAACGCGTCGAGAAGATGCCGAGCGCGTTTTGAGCGAGTGG > NC_002947/2211756‑2212051 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |