Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,264,120	C→T	100%	P24S (CCT→TCT)	accD →	acetyl‑CoA carboxylase carboxyltransferase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,264,120	0	C	T	100.0%	150.9 / NA	45	P24S (CCT→TCT)	accD	acetyl‑CoA carboxylase carboxyltransferase subunit beta
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/24);  total (21/24)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATAAAAGATTTTGAGCTCAAGGGCAGGGCAGGGCCGGTGACGCCGTCCCCCGCCTGCCAATACTGGAGAAAGAAAGCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGCCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTGGAAAAGACCCTGGATGTCTGCCCCAAGTGCAATCACCACATGCGCATCGGCGCACGTGCGCGCATCGACATCTTCCTTGACGCCG  >  NC_002947/2263974‑2264264                                                                                                                                                   |                                                                                                                                                 aTAAAAGATTTTGAGCTCAAGGGCAGGGCAGGGCCGGTGACGCCGTCCCCCGCCTGCCAATACTGGAGAAAGAAAGCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCt                                                                                                                                                <  2:265160/149‑1 (MQ=255)  tAAAAGATTTTGAGCTCAAGGGCAGGGCAGGGCCGGTGACGCCGTCCCCCGCCTGCCAATACTGGAGAAAGAAAGCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCt                                                                                                                                                >  6:162586/1‑148 (MQ=255)  tAAAAGATTTTGAGCTCAAGGGCAGGGCAGGGCCGGTGACGCCGTCCCCCGCCTGCCAATACTGGAGAAAGAAAGCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCt                                                                                                                                                >  3:84881/1‑148 (MQ=255)    aaaGATTTTGAGCTCAAGGGCAGGGCAGGGCCGGTGACGCCGTCCCCCGCCTGCCAATACTGGAGAAAGAAAGCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGa                                                                                                                                              <  2:103634/148‑1 (MQ=255)                 tCAAGGGCAGGGCAGGGCCGGTGACGCCGTCCCCCGCCTGCCAATACTGGAGAAAGAAAGCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAg                                                                                                                                             <  7:124971/136‑1 (MQ=255)                 tCAAGGGCAGGGCAGGGCCGGTGACGCCGTCCCCCGCCTGCCAATACTGGAGAAAGAAAGCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAg                                                                                                                                             >  8:124971/1‑136 (MQ=255)                 tCAAGGGCAGGGCAGGGCCGGTGACGCCGTCCCCCGCCTGCCAATACTGGAGAAAGAAAGCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGcac                                                                                                                                 >  7:293872/1‑148 (MQ=255)                   aaGGGCAGGGCAGGGCCGGTGACGCCGTCCCCCGCCTGCCAATACTGGAGAAAGAAAGCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAg                                                                                                                              <  8:293872/149‑1 (MQ=255)                      ggcagggcagggcCGGTGACGCCGTCCCCCGCCTGCCAATACTGGAGAAAGAAAGCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGc                                                                                                                           >  8:33795/1‑149 (MQ=255)                        cagggcagggcCGGTGACGCCGTCCCCCGCCTGCCAATACTGGAGAAAGAAAGCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGccc                                                                                                                         <  8:240560/149‑1 (MQ=255)                              agggcCGGTGACGCCGTCCCCCGCCTGCCAATACTGGAGAAAGAAAGCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGt                                                                                                                             >  8:275351/1‑139 (MQ=255)                              agggcCGGTGACGCCGTCCCCCGCCTGCCAATACTGGAGAAAGAAAGCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGt                                                                                                                             <  7:275351/139‑1 (MQ=255)                                          gCCGTACACAGCCTGCAAATACTGGATAAAGAAAGAAAGAGCAACTGGTTAGTCTACAAACTTATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCTGTGTCTGATGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCt                                                                                                       <  7:234272/149‑1 (MQ=255)                                           ccGTCCCCCGCCTGCCAATACTGGAGAAAGAAAGCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTc                                                                                                                      <  6:309241/133‑1 (MQ=255)                                           ccGTCCCCCGCCTGCCAATACTGGAGAAAGAAAGCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTc                                                                                                                      >  5:309241/1‑133 (MQ=255)                                                  ccGCCTGCCAATACTGGAGAAAGAAAGCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTc                                                                                               <  6:12557/149‑1 (MQ=255)                                                      cTGCCAATACTGGAGAAAGAAAGCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCg                                                                                             >  8:153823/1‑147 (MQ=255)                                                      cTGCCAATACTGGAGAAAGAAAGCATGAGCAACCGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCg                                                                                             >  4:94055/1‑147 (MQ=255)                                                        gCCAATACTGGAGAAAGAAAGCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAg                                                                                                                                             <  5:201900/97‑1 (MQ=255)                                                        gCCAATACTGGAGAAAGAAAGCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAg                                                                                                                                             >  6:201900/1‑97 (MQ=255)                                                             tACTGGAGAAAGAAAGCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGAGCCTGTGGCACAAGTGCCCGTCCCGCGAGGCCGTGCTGTATCGTCCGGAGCTGGaa                                                                                    <  3:129119/149‑1 (MQ=255)                                                               cTGGAGAAAGAAAGCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTGGaaa                                                                                   <  6:174639/148‑1 (MQ=255)                                                                  gagaAAGAAAGCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTGGAAAAGa                                                                                <  7:33795/148‑1 (MQ=255)                                                                     aaagaaagCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTGGAAAAGACCCt                                                                            <  8:40325/149‑1 (MQ=255)                                                                      aagaaagCATGAGCAACTTGTTAGTCGACAAACTGATACCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTGGAAAAGACCCt                                                                            <  2:66642/148‑1 (MQ=255)                                                                      aagaaagCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTGGAAAAGACCCTg                                                                           <  5:162586/149‑1 (MQ=255)                                                                                 aGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTGGAAAAGACCCTGGATGTCTGccc                                                                >  6:268518/1‑149 (MQ=255)                                                                                  gCAACCGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTGGAAAAGACCCTGGATGTCTGcccc                                                               <  3:94055/149‑1 (MQ=255)                                                                                     aCTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTGGAAAAGACCCTGGATGTCTGCCCCAAg                                                            <  4:84881/149‑1 (MQ=255)                                                                                      cTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTGGAAAAGACCCTGGATGTCTGCCCCAAGt                                                           <  5:268518/149‑1 (MQ=255)                                                                                              tCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTGGAAAAGACCCTGGATGTCTGCCCCAAGTGCAATcac                                                   <  7:153823/149‑1 (MQ=255)                                                                                                gACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTGGAAAAGACCCTGGATGTCTGCCCCAAGTGCAATcacca                                                 >  7:239405/1‑149 (MQ=255)                                                                                                 aCAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTGGAAAAGACCCTGGATGTCTGCCCCAAGTGCAATcacca                                                 >  7:138484/1‑148 (MQ=255)                                                                                                            ccTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTGGAAAAGACCCTGGATGTCTGCCCCAAGTGCAATCACCACATGCGCATCgg                                     >  7:143572/1‑149 (MQ=255)                                                                                                             cTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTGGAAAAGACCCTGGATGTCTGCCCCAAGTGCAATCACCACATgcgcgcacgt                                    >  3:198438/1‑143 (MQ=255)                                                                                                                        cGTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTGGAAAAGACCCTGGATGTCTGCCCCAAGTGCAATCACCACATGCGCATCGGCGCACGTgcgc                          <  8:138484/148‑1 (MQ=255)                                                                                                                         gTTCCGAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTGGAAAAGACCCTGGATGTCTGCCCCAAGTGCAATCACCACATGCGCATCGGCGCACGTgcgcg                         >  6:299112/1‑148 (MQ=255)                                                                                                                              gAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTGGaaaa                                                                                  <  5:18971/86‑1 (MQ=255)                                                                                                                              gAGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTGGaaaa                                                                                  >  6:18971/1‑86 (MQ=255)                                                                                                                               aGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTGGAAAAGACCCTGGATGTCTGCCCCAAGTGCAAt                                                      >  4:249710/1‑113 (MQ=255)                                                                                                                               aGGTGAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTGGAAAAGACCCTGGATGTCTGCCCCAAGTGCAAt                                                      <  3:249710/113‑1 (MQ=255)                                                                                                                                   gAAAAAGAGCTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTGGAAAAGACCCTGGATGTCTGCCCCAAGTGCAATCACCACATGCGCATCGGCGCACGTGCGCGCATCGACATCt              >  5:208961/1‑149 (MQ=255)                                                                                                                                            cTCGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTGGAAAAGACCCTGGATGTCTGCCCCAAGTGCAATCACCACATGCGCATCGGCGCACGTGCGCGCATCGACATCTTCCTTGACg     >  4:323162/1‑149 (MQ=255)                                                                                                                                              cGGTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTGGAAAAGACCCTGGATGTCTGCCCCAAGTGCAATCACCACATGCGCATCGGCGCACGTGCGCGCATCGACATCTTCCTTGACGcc   >  2:171609/1‑149 (MQ=255)                                                                                                                                               ggTGTCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTGGAAAAGACCCTGGATGTCTGCCCCAAGTGCAATCACCACATGCGCATCGGCGCACGTGCGCGCATCGACATCTTCCTTGACGCCg  <  3:323162/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                 ATAAAAGATTTTGAGCTCAAGGGCAGGGCAGGGCCGGTGACGCCGTCCCCCGCCTGCCAATACTGGAGAAAGAAAGCATGAGCAACTGGTTAGTCGACAAACTGATCCCTTCGATCATGCGTTCCGAGGTGAAAAAGAGCTCGGTGCCTGAGGGCCTGTGGCACAAGTGCCCGTCCTGCGAGGCCGTGCTGTATCGTCCGGAGCTGGAAAAGACCCTGGATGTCTGCCCCAAGTGCAATCACCACATGCGCATCGGCGCACGTGCGCGCATCGACATCTTCCTTGACGCCG  >  NC_002947/2263974‑2264264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑